This study aimed to develop an Augmented Reality Pop-Up Diorama as an interactive ecosystem learning medium to enhance students’ biodiversity literacy. The research method used was research and development with the ADDIE model, consisting of the analysis, design, development, implementation, and evaluation stages. The results showed that the medium was categorized as highly valid with an average score of 93.75%. The teacher response reached 95.00%, while the student response reached 90.70%. The students’ average score increased from 51.23 on the pretest to 85.77 on the posttest, with an N-Gain score of 0.71, which was categorized as high. This study concludes that the Augmented Reality Pop-Up Diorama is valid, practical, and effective in supporting ecosystem learning and improving students’ biodiversity literacy. Keywords: Augmented Reality, Biodiversity Literacy, Ecosystem Learning, Interactive Learning Media, Pop-Up Diorama
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