Bioma : Jurnal Ilmiah Biologi
Vol. 15 No. 2 (2026): October 2026

EFFECT OF DRYING TEMPERATURE ON ANTIOXIDANT ACTIVITY, TOTAL PHENOLIC CONTENT, AND TOTAL FLAVONOID CONTENT OF Chrysanthemum morifolium L. Flowers

Ambar Pratiwi (Universitas Ahmad Dahlan)
Lulu Fitriani (Laboratorium Struktur Fisiologi Tumbuhan, Fakultas Sains dan Teknologi Terapan, Univeritas Ahmad Dahlan)
Novi Febrianti (Program Studi Pendidikan Biologi, Fakultas Keguruan Ilmu Pendidikan, Universitas Ahmad Dahlan Jl. Ringroad Selatan, Tamanan, Bnguntapan, Bantul, Yogyakarta)
Eri Eryati (Laboratorium Struktur dan Fisiologi Hewan, Fakultas Sains dan Teknologi Terapan, Univeritas Ahmad Dahlan)



Article Info

Publish Date
08 Oct 2026

Abstract

This study explores the effects of drying temperatures (40, 50, and 60°C) on the extraction yield, antioxidant potential, total phenolic content, and total flavonoid content of Chrysanthemum flower simplicia. Four sample groups were evaluated: fresh flowers and flowers dried at 40, 50, or 60°C. The antioxidant activity was analysed by the DPPH (2,2-diphenyl-1-picrylhydrazyl) method, with IC₅₀ values reported. The Folin- Ciocalteu assay measured total phenolic content and was reported as mg gallic acid equivalents per gram of extract (mg GAE/g). Flavonoids per gram of extract were reported in mg quercetin equivalents (mg QE/g), measured using the AlCl₃ colourimetric technique. The data were analysed statistically through one-way ANOVA, followed by Duncan’s Multiple Range Test, with a significance level of p < 0.05. The fresh control produced a wet-basis extract yield of 0.82%, whereas the dried samples produced dry-basis yields of 3.0%, 3.2%, and 5.1% at 40, 50, and 60 °C, respectively. The highest antioxidant activity occurred in the 40°C group, with the lowest IC₅₀ value of 17.83 ppm. The total phenolic content peaked at 60°C, indicating a value of 124.0 ± 1.59 mg GAE/g extract. TFC differed significantly across the four sample groups because the fresh control was lower than all dried groups; however, the 40, 50, and 60°C drying treatments did not differ significantly from one another.

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Journal Info

Abbrev

bioma

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology

Description

Jurnal Ilmiah Biologi focuses on the publication of result’s research related with the major of biology and biology education. For the major of biology, including systematics, physiology and metabolism, ecology, bioconservation, cell and developmental biology, microbiology, genetics, molecular ...