Background: Menstruation is an important physiological process for young women who need adequate knowledge to maintain reproductive hygiene and health. This study aims to analyze the difference in the proportion of knowledge about menstruation between adolescent girls in rural and urban areas. Method : This comparative cross-sectional research was conducted at SMPN 1 Simo (urban) and SMPN 1 Kemusu (rural) by involving 150 students selected through clustered sampling techniques. Data was collected using a closed-ended questionnaire that had been tested for validity and reliability, with total scores categorized based on the mean value. Univariate analysis was used to describe respondent characteristics, while bivariate analysis used chi square test with continuity correction to compare the proportion of knowledge. Result : The results showed that 59.3% of respondents had good category knowledge, with the proportion of good knowledge higher in urban areas (75.3%) than rural areas (42.5%) and statistically significant differences (p=0.001; OR = 4.1). Implication : These findings show the important role of access to information, health literacy and social environment in shaping menstrual knowledge in rural and urban areas Novelty : This study is one of the comparative studies that specifically compares the proportion of menstrual knowledge between adolescent girls in urban and rural areas in the SMPN 1 Simo and SMPN 1 Kemusu areas using clustered sampling techniques and chi square tests with continuity correction
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