This study aims to determine: 1) improvement of science learning outcomes of seventh grade students through the application of differentiated learning assisted by E-worksheet; 2) improvement of each indicator of science learning outcomes; and 3) significant differences in science learning outcomes before and after implementation. This research used pre-experiment method with One Group Pretest-Posttest design. The population was 34 seventh grade students of SMP IT Wahdah Islamiyah Makassar. The sample, taken by random sampling, obtained class VII A.I with 17 students. Data were collected through multiple choice tests, then analyzed using descriptive and inferential statistics. The results showed that: 1) the application of differentiated learning assisted by E-worksheet improves science learning outcomes with an N-Gain of 0.42 in the moderate category; 2) the increase in each indicator is in the moderate category with an N-Gain on C1 (Remembering) of 0.36; C2 (Understanding) 0.37; C3 (Applying) 0.52; and indicators of determining experimental variables, hypotheses, problem formulations of 0.41 each and experimental objectives of 0.47. The C4 (Analyzing) indicator has an N-Gain of 0.25 and concluding experimental results of 0.18, both in the low category; 3) there are significant differences in science learning outcomes before and after implementation.
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