Pengaruh pemberian serbuk daun salam (Syzygium polyanthum) terhadap kadar hemoglobin pada tikus wistar model anemia

Rhisma Mutiara Devi (Universitas Sebelas maret)
Rizky Alfiqi Nuriza (Departemen Gizi, Poltekkes Kemenkes Surabaya)
Dany Permana (Sekolah Tinggi Ilmu Kesehatan Cianjur)
Baarizah Febriana Badri (Universitas Kusuma Husada Surakarta)
Azzahra Nadya Putri (Poltekkes Kemenkes Bandung)
Suryo Ediyono (Universitas Sebelas Maret)

Holistik Jurnal Kesehatan
Vol. 20 No. 7 (2026): Volume 20 Nomor 7

Background: Anemia remains a major global public health problem, particularly among women of reproductive age. The prevalence of anemia in this population ranges from 27% to 32.8%. In Indonesia, anemia prevalence remains high, affecting 48.9% of adolescent girls and 46.9% of pregnant women. Iron deficiency due to inadequate dietary iron intake and impaired iron absorption is the primary cause of anemia. Indonesian bay leaf (Syzygium polyanthum) powder is a locally available food resource containing iron and has the potential to be developed as an alternative nutritional intervention to improve hemoglobin levels. Purpose: To evaluate the effect of Syzygium polyanthum leaf powder supplementation on hemoglobin levels in anemic female Wistar rats. Method: A pretest–posttest controlled experimental design conducted at the Integrated Research and Testing Laboratory, Universitas Gadjah Mada, and the Integrated Laboratory of Universitas Sebelas Maret from August to October 2023. Six-week-old female Wistar rats (Rattus norvegicus) weighing approximately 150 g were obtained from UD Wistar, Yogyakarta. During the acclimatization period, the rats were fed commercial feed and an iron-deficient AIN-93M diet. Anemia was induced by administering an iron-deficient diet for 15 days. Subsequently, Syzygium polyanthum leaf powder was orally administered for 28 days at three different doses: P1 (18.6 mg/200 g body weight), P2 (36 mg/200 g body weight), and P3 (55.6 mg/200 g body weight). Hemoglobin levels were measured using the cyanmethemoglobin method. Results: Administration of Syzygium polyanthum leaf powder for 28 days significantly increased hemoglobin levels. The greatest improvement was observed in the P1 group (18.6 mg/200 g body weight), with a mean hemoglobin increase of 1.16 ± 1.34 g/dL. Conclusion: Supplementation with Syzygium polyanthum leaf powder at a dose of 18.6 mg/200 g body weight significantly improved hemoglobin levels in anemic female Wistar rats. Syzygium polyanthum leaf powder has potential as an alternative intervention for anemia; however, further studies are required to confirm its effectiveness.   Keywords: Anemia; Hemoglobin; Rattus norvegicus; Syzygium polyanthum; Wistar Rats.