The marble goby (Oxyeleotris marmorata) is a high-value freshwater fish whose increasing exploitation from natural waters may threaten resource sustainability, particularly when undersized individuals are harvested. Domestication through controlled rearing and improved feed management is therefore needed to support conservation while increasing the economic value of captured fish. This study evaluated the growth performance and feed utilization of marble goby fed trash fish enriched with effective microorganisms (EM4) at different doses. The experiment was conducted for 56 days using 20 wild-caught marble goby weighing less than 500 g and reared in four tarpaulin ponds with four feed treatments: 0, 10, 15, and 20 mL EM4 kg⁻¹ trash fish. Growth, feed intake rate, feed conversion ratio, specific growth rate, survival rate, and water quality were evaluated descriptively. The 10 mL kg⁻¹ treatment produced the most balanced response, with the highest weight gain, cumulative specific growth rate, and 100% survival, whereas 15 mL kg⁻¹ produced the greatest length gain. The 20 mL kg⁻¹ treatment did not improve performance proportionally and showed lower weight gain and survival. Thus, 10 mL EM4 kg⁻¹ trash fish is the most promising treatment under the experimental conditions and provides an initial basis for conservation-oriented domestication of marble goby
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