<![CDATA[Central Kalimantan is a significant pineapple-growing area in Indonesia, where cultivated pineapples exhibit morphological differences across production sites. This research analyzed sequence variation in local pineapple (Ananas comosus) utilizing the chloroplast rbcL marker. Six samples were gathered from Central Kalimantan and East Java, and two A. comosus sequences from Johor, Malaysia, were retrieved from GenBank for comparison. Genomic DNA was extracted, the rbcL region was amplified and sequenced, and the obtained sequences were aligned and examined using pairwise p-distance and Neighbor-Joining (NJ) analysis with the Kimura 2-parameter (K2P) distance model and 1,000 bootstrap replications. Pairwise distances among the samples created in this research were around 0.00–0.01%, while distances between the study samples and the Malaysian reference sequences were roughly 0.06%. The alignment indicated that the rbcL region was very conserved, exhibiting minimal nucleotide variation and an indel found in a single Malaysian reference sequence (AC7_NCBI). The sequences produced in this research grouped tightly, suggesting minimal rbcL sequence variation among the collected cultivated pineapples. Since the Malaysian sequences belong to the same species as the study samples and no external taxon was utilized as an outgroup, the resulting tree is viewed as an unrooted sequence-clustering analysis instead of a rooted phylogeny. The findings suggest that rbcL by itself has restricted ability to differentiate local pineapple accessions and needs to be enhanced with more variable markers, like matK and/or nuclear ITS, along with wider sampling.]]>
Copyrights © 2026
