<![CDATA[Temperature and relative humidity (%RH) are significant variables that affect the composition of airborne bacterial communities. The presence of airborne bacteria in a research laboratory is crucial to investigate, as it affects the laboratory safety. Therefore, the purpose of this study was to examine the effects of temperature and %RH on the structure of airborne bacterial communities in the laboratory and compare the diversity and abundance of airborne bacteria in two research laboratories with different operational characteristics. The exposure plate procedure was used to collect airborne bacterial samples. The types and numbers of bacterial colonies that grew on the media served as data for the abundance and diversity of airborne bacteria, which were evaluated with the statistical analysis software PAST4. The most dominant types of airborne bacteria in both laboratories were identified using 16S rRNA gene sequences. Temperature and %RH data were processed using the GraphPad Prism. According to the findings of this study, the Microbiology Laboratory (LM) has a higher average temperature and a lower %RH than the Biomolecular Laboratory (LB). The average temperature and %RH in the morning (LM: 27.4 oC/ 77.3% and LB: 26.6 oC/ 81.1%) and afternoon (LM: 27.6 oC /76.1% and LB: 26.9 oC/ 79.2%), were not significantly different. However, there were notable differences in the morning and afternoon temperatures and %RH between LM and LB. LM showed greater airborne bacterial diversity than LB, whereas LB exhibited higher bacterial abundance than LM. Gram-positive bacterial species predominated in the airborne bacterial populations in LM and LB. Brevibacterium and Micrococcus were the most common airborne bacteria found in LM and LB, respectively. This finding is intended to provide guidelines for biosafety management and control, particularly in research facilities]]>
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