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All Journal Adi Widya : Jurnal Pengabdian Masyarakat Bioscientist : Jurnal Ilmiah Biologi Jurnal Laboratorium Khatulistiwa
Inayah, Mazidah Noer
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Other

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PENINGKATAN KOMPETENSI MENULIS BAGI MAHASISWA POLITEKNIK YAKPERMAS BANYUMAS MELALUI PELATIHAN PENULISAN ARTIKEL ILMIAH Inayah, Mazidah Noer; Budiarti, Suci Indah; Widyayanti, Oksita Asri; Nurcahyanti, Khusnul Khotimah Arum; Rahmansyah, Ilham; Alifah, Assha Luthfianie
Adi Widya : Jurnal Pengabdian Masyarakat Vol 9 No 2 (2025): Adi Widya: Jurnal Pengabdian Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33061/awpm.v9i2.13081

Abstract

Menulis artikel ilmiah seringkali menjadi tantangan tersendiri bagi mahasiswa. Beberapa kesulitan yang dihadapi mahasiswa dalam menulis diantaranya membuat parafrase kalimat, menyajikan dan menjelaskan data hasil penelitian, terbatasnya referensi, hingga rendahnya motivasi dalam menulis. Oleh karena itu, diperlukan kegiatan pelatihan penulisan artikel ilmiah bagi mahasiswa dengan tujuan memberikan pengetahuan dan pemahaman (edukasi) serta melatih keterampilan  menulis artikel ilmiah dengan baik agar menghasilkan publikasi ilmiah yang berkualitas serta bermanfaat. Kegiatan pelatihan penulisan artikel ilmiah bagi mahasiswa tingkat akhir di Politeknik Yakpermas Banyumas diselenggarakan dalam 2 hari. Hari pertama yaitu penyampaian materi tentang artikel ilmiah, kemudian dilanjutkan hari kedua yaitu asistensi penulisan artikel ilmiah. Pelatihan ini diikuti oleh 85 mahasiswa tingkat akhir yang berasal dari tiga program studi yaitu D-III Keperawatan, D-III Rekam Medis dan Informasi Kesehatan, dan D-IV Teknologi Laboratorium Medis. Sebanyak 5 orang peserta dengan artikel ilmiah terbaik diberikan apresiasi berupa penerbitan artikel pada Journal of Nursing and Health.
Isolasi dan Identifikasi Molekuler Bakteri Salmonella Asal Tempe Dage Berdasarkan Gen 16S rRNA Inayah, Mazidah Noer; Budiarti, Suci Indah; Wulan, Rahayu
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18200

Abstract

This study aimed to isolate Salmonella from empe dage, particularly products sold in traditional markets in the Banyumas region, Central Java, and to identify the isolates through molecular analysis of the 16S rRNA gene. Isolation of Salmonella from dage samples was conducted using culture-based methods on several types of selective media specific for Salmonella. Identification was performed through biochemical characterization using the API 20E system and molecular analysis based on PCR amplification of the 16S rRNA gene. The resulting 16S rRNA sequences were subsequently analyzed using the BLAST tool available on the NCBI platform, and phylogenetic relationships were reconstructed with MEGA X. The study successfully obtained one bacterial isolate (TD.1), exhibiting transparent colonies with black centers on SSA medium. Biochemical tests including catalase activity, sugar fermentation, H₂S production, and citrate utilization showed positive results. Further identification using the API 20E system confirmed that isolate TD.1 belonged to the genus Salmonella. Molecular analysis of the 16S rRNA gene identified isolate TD.1 as Salmonella enterica. In conclusion, isolate TD.1, recovered from tempe dage was identified as Salmonella enterica. The presence of Salmonella enterica in dage highlights a potential health risk and underscores the need for improved food hygiene in traditional markets.
Kandungan Formalin dan Jumlah Total Bakteri pada Produk Sosis di Pasar Tradisional Moga Kabupaten Pemalang Jawa Tengah Alimi, Ramadan Nur; Ambarwanty Mohtar, Cici Farhana; Inayah, Mazidah Noer
Jurnal Laboratorium Khatulistiwa Vol 9, No 1 (2025): November 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i1.2091

Abstract

One sort of frozen food that is made from processed meat is sausage. Formalin is one of preservative that is frequently abused, which can found in market-sold sausages. Health issues can arise from consuming foods containing formalin for an extended period of time. The filthy market environment, dust, and touch between customers' and sellers' hands can all lead to microbial contamination of food that sold in traditional markets. There has never been any prior research on the formalin content and bacterial contamination of sausage products sold in Pemalang Central Java's traditional markets.  Therefore, the purpose of this study was to figure out the amount of formalin and the quantity of bacteria contaminating sausage products that are sold at Moga traditional market in Pemalang, Central Java.  There were 6 samples of sausage were chosen randomly from several suppliers. A formalin test kit was used to qualitatively analyze the formalin, and the TPC method was employed for counting the bacteria. The result showed that formalin was absent from every sausage products. However, the TPC indicated that 4 of 6 sausages were contaminated with bacteria, S01, A02, S03, and S06, with counts of 1,2 × 10⁴ CFU/mL, 8.6 × 10⁴ CFU/mL, 8.7 × 10⁴ CFU/mL, and 3 × 10⁴ CFU/mL, respectively. Fortunately, the overall bacterial count remained below the acceptable level for human consumption.
Co-Authors Alifah, Assha Luthfianie Alimi, Ramadan Nur Ambarwanty Mohtar, Cici Farhana Budiarti, Suci Indah Nurcahyanti, Khusnul Khotimah Arum Rahmansyah, Ilham Widyayanti, Oksita Asri WULAN, RAHAYU