Article Per Year (5 Year)
p-Index From 2021 - 2026
0.659
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences BIOTROPIC The Journal of Tropical Biology Journal of Biomedical Sciences and Health
Alfisah, Rizal Khoirun
Program Studi Biologi, Fakultas Biologi, Universitas Jenderal Soedirman, Purwokerto, Indonesia
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
Characteristics and Activity of Anti Qourum Sensing Bacillus spp. Isolated from Penaeus vannamei Shrimp Ponds Iman Rusmana; Yuni Puji Hastuti; Rizal Khoirun Alfisah; Alina Akhdiya
HAYATI Journal of Biosciences Vol. 29 No. 1 (2022): January 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.1.97-106

Abstract

Certain strains of V. parahaemolyticus carry a gene that encodes a toxin that causes Acute hepatopancreatic necrosis disease (AHPND) in P. vannamei. AHPND attacks shrimp post larvae within 20-30 days after stocking causing up to 100% mortality. The expression of these virulent genes is controlled by the quorum sensing system. This system is inhibited by an anti-quorum sensing (AQS) mechanism. Several Bacillus strains have AQS mechanism by producing AHL-Lactonase enzyme. Therefore, this study aimed to obtain Bacillus spp. having AQS activity for controlling AHPND. The study was conducted from isolation and selection of Bacillus isolates, as well as determination of AQS activity. From 22 samples consisting of shrimp intestines, water and pond sediment samples, a total of 151 isolates of Bacillus spp. were isolated. The screening test for AQS activity obtained 11 isolates that showed AQS activity on Cromobacterium violaceum. Determination of violacein pigment in liquid cultures of C. violaceum showed the index value of the pigment formation was between 0.025-0.166 and 0.026-0.567 at 24-hour and between 48-hour incubations, respectively. The quantitative analysis of violacein production showed that there were six isolates of Bacillus could inhibit the pigment production more than 75%. The isolates were identified as Bacillus cereus (four isolates), Bacillus thuringiensis (one isolate), and Bacillus velezensis (one isolate), respectively. The molecular analysis had confirmed that the isolates have aiiA genes encoding AHL-lactonase enzyme. These Bacillus isolates have potential application for controlling AHNPD disease.
Keragaman Morfologi Bakteri Nitrifikasi Asal Kompos Kotoran Domba pada Peternakan Domba dengan Sistem Bedding Sri Martina Wiraswati; Hendro Pramono; Oedjijono; Dini Ryandini; Dyah Fitri Kusharyati; Meyta Pratiwi; Taruna Dwi Satwika; Dwiana Muflihah Yulianti; Saefuddin Aziz; Daniel Joko Wahyono; Rizal Khoirun Alfisah; Yuriza Eshananda; Budi Rustomo; Afifah Mariana
Biotropic : The Journal of Tropical Biology Vol. 7 No. 1 (2023): Biotropic, Volume 7 Nomor 1, 2023
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v7i1.1695

Abstract

The application of compost bedding system on sheep farm is a technology to minimize the negative impact of sheep manure waste toward environment. The microorganisms which inhabit the compost bedding, especially nitrifying bacteria are expected to have significant role in odor emission reduction which become environmental problem around the sheep farm. This study aims to analyze the abundance and diversity of nitrifying bacteria of compost bedding obtained from sheep farm which have important role in reducing odor emission of ammonia. The 12 days, 1 and 1,5 months old of compost bedding samples were obtained from sheepfold, while 3 and 4 months old of compost samples were obtained from compost pile. Furthermore, nitrifying bacteria were isolated from compost samples by pour plate method using specific media for Nitrosomonas and Nitrobacter. The results indicated that compost bedding samples with different composting time duration displayed different abundance and diversity of nitrifying bacteria. The current study was successfully isolated 39 and 47 nitrifying bacterial isolates using specific media for Nitrobacter and Nitrosomonas, respectively. The highest diversity of nitrifying bacteria was gained from 12 days old compost bedding sample. Nitrifying bacterial isolates from compost bedding samples have significant role in odor emission declining as well as manure composting at sheep farm. The obtained bacterial isolates are also potentially to develop as bio-activator for compost bedding.
Enhanced Survival of Lactobacillus Lg71 from Mangrove Sediment Under Simulated Gastric and Intestinal Conditions Kusharyati, Dyah Fitri; Satwika, Taruna Dwi; Alfisah, Rizal Khoirun; Mariana, Afifah; Rovik, Anwar; Oedjijono, Oedjijono
Journal of Biomedical Sciences and Health Vol. 3 No. 1 (2026)
Publisher : Universitas Karya Husada Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34310/jbsh.v3.i1.291

Abstract

Background: For a probiotic to offer health benefits, it must stay viable through the tough environment of the human gastrointestinal tract. This study assesses the effectiveness of sodium alginate encapsulation in improving the survival of Lactobacillus LG71, a new strain from mangrove sediment, under simulated gastric and intestinal conditions. Objective: This study aims to evaluate the effectiveness of sodium alginate encapsulation in enhancing the viability and survival of Lactobacillus LG71, a probiotic strain isolated from mangrove sediment, during cold storage and under simulated gastric and intestinal conditions. Methods: Lactobacillus LG71 was encapsulated in sodium alginate beads. The viability of both encapsulated and free (non-encapsulated) cells was assessed over a 4-week storage period at 4 °C and during sequential exposure to simulated gastric and intestinal environments. Results: Encapsulation significantly improved survival rates compared to free cells (p < 0.05). Although both groups experienced a decline during the first week of storage, encapsulated cells maintained a high viability of approximately 107 CFU/mL, losing only 2.51 log CFU/g over four weeks. Most notably, encapsulated Lactobacillus LG71 demonstrated greater resilience during digestion, retaining populations of 2.21 log CFU/mL in gastric simulations and 1.00 log CFU/mL in intestinal simulations. In contrast, free cells were much more vulnerable to these acidic and enzymatic conditions. Conclusion: Sodium alginate encapsulation effectively protects Lactobacillus LG71 from environmental and biological stressors. These findings indicate that encapsulation is a crucial step for the commercial application of mangrove-derived probiotics, ensuring that a viable dose of living cells reaches the host's lower gastrointestinal tract to promote health benefits.
Co-Authors Afifah Mariana Alina Akhdiya Budi Rustomo Daniel Joko Wahyono Dini Ryandini Dwiana Muflihah Yulianti Dyah Fitri Kusharyati Dyah Fitri Kusharyati Hendro Pramono Iman Rusmana Mariana, Afifah Meyta Pratiwi Oedjijono Oedjijono Oedjijono, Oedjijono Rovik, Anwar Saefuddin Aziz Satwika, Taruna Dwi Sri Martina Wiraswati Taruna Dwi Satwika Yuni Puji Hastuti Yuriza Eshananda