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All Journal HAYATI Journal of Biosciences JRST (Jurnal Riset Sains dan Teknologi) Journal of Biomedical Sciences and Health BIOTROPIC
Alfisah, Rizal Khoirun
Program Studi Biologi, Fakultas Biologi, Universitas Jenderal Soedirman, Purwokerto, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Engineering Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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 1 
Characteristics and Activity of Anti Qourum Sensing Bacillus spp. Isolated from Penaeus vannamei Shrimp Ponds Iman Rusmana; Yuni Puji Hastuti; Rizal Khoirun Alfisah; Alina Akhdiya
HAYATI Journal of Biosciences Vol. 29 No. 1 (2022): January 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.1.97-106

Abstract

Certain strains of V. parahaemolyticus carry a gene that encodes a toxin that causes Acute hepatopancreatic necrosis disease (AHPND) in P. vannamei. AHPND attacks shrimp post larvae within 20-30 days after stocking causing up to 100% mortality. The expression of these virulent genes is controlled by the quorum sensing system. This system is inhibited by an anti-quorum sensing (AQS) mechanism. Several Bacillus strains have AQS mechanism by producing AHL-Lactonase enzyme. Therefore, this study aimed to obtain Bacillus spp. having AQS activity for controlling AHPND. The study was conducted from isolation and selection of Bacillus isolates, as well as determination of AQS activity. From 22 samples consisting of shrimp intestines, water and pond sediment samples, a total of 151 isolates of Bacillus spp. were isolated. The screening test for AQS activity obtained 11 isolates that showed AQS activity on Cromobacterium violaceum. Determination of violacein pigment in liquid cultures of C. violaceum showed the index value of the pigment formation was between 0.025-0.166 and 0.026-0.567 at 24-hour and between 48-hour incubations, respectively. The quantitative analysis of violacein production showed that there were six isolates of Bacillus could inhibit the pigment production more than 75%. The isolates were identified as Bacillus cereus (four isolates), Bacillus thuringiensis (one isolate), and Bacillus velezensis (one isolate), respectively. The molecular analysis had confirmed that the isolates have aiiA genes encoding AHL-lactonase enzyme. These Bacillus isolates have potential application for controlling AHNPD disease.
Enhanced Survival of Lactobacillus Lg71 from Mangrove Sediment Under Simulated Gastric and Intestinal Conditions Kusharyati, Dyah Fitri; Satwika, Taruna Dwi; Alfisah, Rizal Khoirun; Mariana, Afifah; Rovik, Anwar; Oedjijono, Oedjijono
Journal of Biomedical Sciences and Health Vol. 3 No. 1 (2026)
Publisher : Universitas Karya Husada Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34310/jbsh.v3.i1.291

Abstract

Background: For a probiotic to offer health benefits, it must stay viable through the tough environment of the human gastrointestinal tract. This study assesses the effectiveness of sodium alginate encapsulation in improving the survival of Lactobacillus LG71, a new strain from mangrove sediment, under simulated gastric and intestinal conditions. Objective: This study aims to evaluate the effectiveness of sodium alginate encapsulation in enhancing the viability and survival of Lactobacillus LG71, a probiotic strain isolated from mangrove sediment, during cold storage and under simulated gastric and intestinal conditions. Methods: Lactobacillus LG71 was encapsulated in sodium alginate beads. The viability of both encapsulated and free (non-encapsulated) cells was assessed over a 4-week storage period at 4 °C and during sequential exposure to simulated gastric and intestinal environments. Results: Encapsulation significantly improved survival rates compared to free cells (p < 0.05). Although both groups experienced a decline during the first week of storage, encapsulated cells maintained a high viability of approximately 107 CFU/mL, losing only 2.51 log CFU/g over four weeks. Most notably, encapsulated Lactobacillus LG71 demonstrated greater resilience during digestion, retaining populations of 2.21 log CFU/mL in gastric simulations and 1.00 log CFU/mL in intestinal simulations. In contrast, free cells were much more vulnerable to these acidic and enzymatic conditions. Conclusion: Sodium alginate encapsulation effectively protects Lactobacillus LG71 from environmental and biological stressors. These findings indicate that encapsulation is a crucial step for the commercial application of mangrove-derived probiotics, ensuring that a viable dose of living cells reaches the host's lower gastrointestinal tract to promote health benefits.
Keragaman Morfologi Bakteri Nitrifikasi Asal Kompos Kotoran Domba pada Peternakan Domba dengan Sistem Bedding Sri Martina Wiraswati; Hendro Pramono; Oedjijono; Dini Ryandini; Dyah Fitri Kusharyati; Meyta Pratiwi; Taruna Dwi Satwika; Dwiana Muflihah Yulianti; Saefuddin Aziz; Daniel Joko Wahyono; Rizal Khoirun Alfisah; Yuriza Eshananda; Budi Rustomo; Afifah Mariana
Biotropic : The Journal of Tropical Biology Vol. 7 No. 1 (2023): Biotropic, Volume 7 Nomor 1, 2023
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v7i1.1695

Abstract

The application of compost bedding system on sheep farm is a technology to minimize the negative impact of sheep manure waste toward environment. The microorganisms which inhabit the compost bedding, especially nitrifying bacteria are expected to have significant role in odor emission reduction which become environmental problem around the sheep farm. This study aims to analyze the abundance and diversity of nitrifying bacteria of compost bedding obtained from sheep farm which have important role in reducing odor emission of ammonia. The 12 days, 1 and 1,5 months old of compost bedding samples were obtained from sheepfold, while 3 and 4 months old of compost samples were obtained from compost pile. Furthermore, nitrifying bacteria were isolated from compost samples by pour plate method using specific media for Nitrosomonas and Nitrobacter. The results indicated that compost bedding samples with different composting time duration displayed different abundance and diversity of nitrifying bacteria. The current study was successfully isolated 39 and 47 nitrifying bacterial isolates using specific media for Nitrobacter and Nitrosomonas, respectively. The highest diversity of nitrifying bacteria was gained from 12 days old compost bedding sample. Nitrifying bacterial isolates from compost bedding samples have significant role in odor emission declining as well as manure composting at sheep farm. The obtained bacterial isolates are also potentially to develop as bio-activator for compost bedding.
Soil-Derived Endospore-Forming Bacillus Bacteria Producing Protease Mazidah Noer Inayah; Suci Indah Budiarti; Rizal Khoirun Alfisah
JRST (Jurnal Riset Sains dan Teknologi) Volume 9 No. 2 September 2025: JRST
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/jrst.v9i2.26600

Abstract

Proteases are widely utilized in many industries, including waste treatment, textiles, detergents, food processing, and medicines. This encourages researchers to discover novel sources of these enzymes. Exploration and isolation of putative proteolytic bacteria from the soil is one promising approach. The purpose of this study is expected providing the bacterial isolates that will produce superior proteases. Proteolytic bacteria were isolated from soil samples using the spread plate technique and selective skim milk agar media. The formation of clear zones and the determination of the proteolytic index were the starting point for a qualitative analysis of protease activity. Protease activity was determined quantitatively. The substrate is 1% casein dissolved in a 0.2 M tris-HCl buffer at pH 7.5, while the standard is tyrosine. The quantitative measurement of protease activity was carried out concurrently with the bacterial growth curve determination. The proteolytic bacteria P02 was successfully isolated in this research, having a protease enzyme activity of 0.867 U/mL and a proteolytic index of 0.8 ± 0.1. Protease activity peaked when the bacterial growth was in the logarithmic phase. Proteolytic bacterial P02 was Gram-positive and possesses the ability to form endospores and has a rod-shaped cell morphology. According to the outcomes of biochemical physiological testing, the bacterial P02 is considered to be the genus of Bacillus sp. The proteolytic bacteria P02 has potential to provide a sustainable and renewable source of protease, which is widely employed in industry and biotechnology.
Studi Komposisi Bakteri dan Mikroalga pada Sistem Akuakultur Air Tawar Meyta Pratiwi; Atang; Aulidya Nurul Habibah; Rizal Khoirun Alfisah
Biotropic : The Journal of Tropical Biology Vol. 10 No. 1 (2026): Biotropic, Volume 10 Nomor 1, 2026
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v10i1.2527

Abstract

Freshwater aquaculture is still in demand by fish farmers to meet consumer demand for animal protein needs. Fish farming uses an intensive system to produce adequate amounts of fish, but it is followed by metabolite waste problems that can affect the quality of life of fish. Handling fish metabolite waste can be started by conducting a study of microbes that play a role in the decomposition of fish metabolite waste. The method used to determine the microbes is the survey method, by isolating culturable bacteria using nutrient agar (NA) and Winogradsky media, identifying microalga found in fish farming ponds, measuring the physico-chemical parameters of aquaculture pond water. The results obtained were five heterotrophic bacteria (106 cfu/mL), five nitrifying bacteria (105 cfu/mL), four phylum microalgae, and physico-chemical conditions of water (temperature 27 °C, TDS 76-78 mg/L, nitrates 2-2.7 mg/L, and pH 6.5-6.7). The microbes obtained are widely known to help the process of decomposing metabolite waste in fish farming ponds, so that they can be used as promised probiotic agents for aquaculture organisms in the future.
Co-Authors Afifah Mariana Alina Akhdiya Atang Aulidya Nurul Habibah Budi Rustomo Daniel Joko Wahyono Dini Ryandini Dwiana Muflihah Yulianti Dyah Fitri Kusharyati Dyah Fitri Kusharyati Hendro Pramono Iman Rusmana Inayah, Mazidah Noer Mariana, Afifah Meyta Pratiwi Meyta Pratiwi Oedjijono Oedjijono Oedjijono, Oedjijono Rovik, Anwar Saefuddin Aziz Satwika, Taruna Dwi Sri Martina Wiraswati Suci Indah Budiarti Taruna Dwi Satwika Yuni Puji Hastuti Yuriza Eshananda