<![CDATA[Background: Increased consumption of foods high in saturated fat and cholesterol can lead to hypercholesterolemia. Although statins are effective in treating hypercholesterolemia, their use may cause adverse effects, creating a need for alternative therapies derived from natural products. Clove mistletoe leaves (Dendrophthoe pentandra (L.) Miq.) contain bioactive compounds, particularly flavonoids, which have potential anti-cholesterol activity. Methods: Extraction was carried out using the sonication method with 70% ethanol as the solvent. The extract was fractionated using three solvents: water, ethyl acetate, and n-hexane. Phytochemical screening was performed using qualitative tube tests, followed by an in vitro anticholesterol assay using the Liebermann-Burchard method. The IC50 value was calculated using linear regression analysis of the relationship curve of concentration versus percentage of cholesterol reduction. Result: The results showed that the ethyl acetate fraction had the highest anticholesterol activity with an IC50 value of 18,043 ppm, followed by the water fraction (22,910 ppm), ethanol extract (41,645 ppm), and n-hexane fraction (85,510 ppm). The IC50 value of the ethyl acetate fraction was the closest to the IC50 value of simvastatin (7,703 ppm) as a positive control. Conclusions: The ethyl acetate fraction showed the highest activity with an IC50 value of 18,043 ppm, while the n-hexane fraction showed the lowest activity with an IC50 value of 85,510]]>
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