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All Journal International Journal of Health, Engineering and Technology Journal of Pharmaceutical and Sciences. Sanitas: Journal of Health, Medical, and Psychological Studie
Rahmat Hidayat
Universitas Duta Bangsa
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Engineering Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Nursing Public Health Veterinary

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Uji Potensi Fraksi n-Heksan Daun Keji Besi (Hemigraphis repanda L.) Sebagai Antibakteri Terhadap Escherichia Coli dan Bacillus Cereus Beserta Penentuan Konsentrasi Hambat Minimum (KHM) Alfina Nurrahman; Rahmat Hidayat; Annora Rizky Amalia
Journal of Health, Medical, and Psychological Studies Vol 1 No 2 (2025): December: Sanitas: Journal of Health, Medical, and Psychological Studies
Publisher : CV SCRIPTA INTELEKTUAL MANDIRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.65310/29v9p164

Abstract

This study aimed to evaluate the antibacterial potential of the n-hexane fraction of Keji Besi leaves (Hemigraphis repanda L.) against Escherichia coli and Bacillus cereus, as well as to determine its Minimum Inhibitory Concentration (MIC). Keji Besi leaves were extracted by maceration using 96% ethanol, followed by liquid–liquid fractionation with n-hexane. The antibacterial activity was assessed using the disc diffusion method, while MIC values were determined using the broth dilution method. Phytochemical screening revealed that the n-hexane fraction predominantly contained steroid and triterpenoid compounds. The disc diffusion assay showed that the n-hexane fraction exhibited stronger inhibitory activity against B. cereus than E. coli, with the largest inhibition zone observed at an 80% concentration. MIC determination indicated that the MIC value for B. cereus was 40% (w/v), whereas E. coli required a higher concentration of 80% (w/v) to inhibit bacterial growth. These findings suggest that the n-hexane fraction of Keji Besi leaves demonstrates selective antibacterial activity, particularly against Gram-positive bacteria. Therefore, this fraction has promising potential to be developed as a natural antibacterial agent, especially for infections caused by Gram-positive pathogens.
Evaluation Of Sun Protection Factor Value Of Water, Ethyl Acetate, And N-Hexane Fractions Of Ethanolic Extract Of Crystal Guava Fruit (Psidium Guajava. L.) By UV-VIS Spectrophotometry Farida Kumalasari; Rahmat Hidayat; Tiara Ajeng Listiyani
International Journal of Health Engineering and Technology Vol. 4 No. 6 (2026): IJHET MARCH 2026
Publisher : CV. AFDIFAL MAJU BERKAH

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55227/ijhet.v4i6.661

Abstract

Excessive exposure to ultraviolet (UV) rays can cause various skin damage, so an effective and safe sunscreen preparation is needed. Natural ingredients, especially crystal guava fruit (Psidium guajava L.), are known to contain secondary metabolite compounds such as flavonoids that have the potential as natural photoprotective agents. Crystal Guava Fruit (Psidium guajava L.) contains several secondary metabolite compounds, namely flavonoids, saponins, and terpenoids. The content of secondary metabolites plays a role as a Sun Protection Factor, because these compounds are able to absorb UV A and UV B rays well. This study aims to determine the Sun Protection Factor (SPF) value of ethyl acetate, n-Hexane, and water fractions from crystal guava fruit (Psidium guajava L.) in vitro using the UV-Vis spectrophotometry method and to determine the fraction with the best sunscreen activity. The extract was prepared by maceration using 96% ethanol solvent, then the extract was fractionated in stages using n-Hexane, ethyl acetate, and water. SPF values ​​were determined in vitro using UV-Vis spectrophotometry at a wavelength of 290-320 nm. Each fraction was prepared in several concentrations: 200, 400, 600, 800, and 1000 ppm. The study showed that all fractions had sunscreen activity with SPF values ​​that increased with increasing concentration. The ethyl acetate fraction showed the highest activity with an SPF value of 24.249 at a concentration of 1000 ppm which is included in the ultra protection category. This study shows that the water, ethyl acetate, and n-Hexane fraction of crystal guava fruit (Psidium guajava L.) have the potential as SPF because they are in the range 2-4 (minimum category).
Uji aktivitas inhibisi enzim α-amilase fraksi daun benalu Cengkeh (Dendrophthoe pentandra (L.) Miq.) secara in vitro sebagai kandidat antidiabetes Husnun Hanifah; Rahmat Hidayat; Annie Rahmatillah
Journal of Pharmaceutical and Sciences JPS Volume 9 Nomor 4 (2026)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v9i4.1899

Abstract

Background: Diabetes mellitus is a metabolic disorder characterized by hyperglycemia. One strategy for controlling postprandial blood glucose levels is through the inhibition of the α-amylase enzyme. Clove mistletoe leaves (Dendrophthoe pentandra (L.) Miq.)  are known to contain flavonoids that have the potential to act as natural α-amylase inhibitors. This study aimed to identify the secondary metabolites, evaluate the α-amylase inhibitory activity, and determine the clove mistletoe leaf fraction with the highest inhibitory activity. Method: The extract was obtained by ultrasonic-assisted extraction using 70% ethanol and subsequently fractionated with n-hexane, ethyl acetate, and water. The α-amylase inhibitory assay was performed using the starch–iodine method and analyzed by UV-Vis spectrophotometry at a wavelength of 575 nm with concentrations of 100, 250, 500, 750, and 1000 ppm. Results: Phytochemical screening revealed that the ethyl acetate fraction contained flavonoids, tannins, alkaloids, and terpenoids. The ethyl acetate fraction exhibited the highest α-amylase inhibitory activity with an IC₅₀ value of 28.794 ppm (very strong), followed by the ethanol extract with an IC₅₀ of 46.756 ppm (very strong), the aqueous fraction with an IC₅₀ of 155.560 ppm (moderate), and the n-hexane fraction with an IC₅₀ of 383.263 ppm (weak), and the metformin with an IC₅₀ of 6,61 ppm (very strong). Conclution: These findings suggest that the ethyl acetate fraction of clove mistletoe leaves has promising potential as an antidiabetic candidate through the inhibition of the α-amylase enzyme.
Uji aktivitas antikolesterol ekstrak serta fraksi daun benalu cengkeh (Dendrophtoe pentandra (L.) Miq.) secara in vitro Cindhan Bingar Widyaswara; Rahmat Hidayat; Danang Raharjo
Journal of Pharmaceutical and Sciences JPS Volume 9 Nomor 4 (2026)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v9i4.1902

Abstract

Background: Increased consumption of foods high in saturated fat and cholesterol can lead to hypercholesterolemia. Although statins are effective in treating hypercholesterolemia, their use may cause adverse effects, creating a need for alternative therapies derived from natural products. Clove mistletoe leaves (Dendrophthoe pentandra (L.) Miq.) contain bioactive compounds, particularly flavonoids, which have potential anti-cholesterol activity. Methods: Extraction was carried out using the sonication method with 70% ethanol as the solvent. The extract was fractionated using three solvents: water, ethyl acetate, and n-hexane. Phytochemical screening was performed using qualitative tube tests, followed by an in vitro anticholesterol assay using the Liebermann-Burchard method. The IC50 value was calculated using linear regression analysis of the relationship curve of concentration versus percentage of cholesterol reduction. Result: The results showed that the ethyl acetate fraction had the highest anticholesterol activity with an IC50 value of 18,043 ppm, followed by the water fraction (22,910 ppm), ethanol extract (41,645 ppm), and n-hexane fraction (85,510 ppm). The IC50 value of the ethyl acetate fraction was the closest to the IC50 value of simvastatin (7,703 ppm) as a positive control. Conclusions: The ethyl acetate fraction showed the highest activity with an IC50 value of 18,043 ppm, while the n-hexane fraction showed the lowest activity with an IC50 value of 85,510
Co-Authors Alfina Nurrahman Amalia, Annora Rizky Annie Rahmatillah Cindhan Bingar Widyaswara Danang Raharjo Farida Kumalasari Husnun Hanifah Tiara Ajeng Listiyani