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All Journal International Journal of Health, Engineering and Technology Sanitas: Journal of Health, Medical, and Psychological Studie
Rahmat Hidayat
Universitas Duta Bangsa
Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Dentistry Engineering Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Mechanical Engineering Medicine & Pharmacology Nursing Public Health Veterinary

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Uji Potensi Fraksi n-Heksan Daun Keji Besi (Hemigraphis repanda L.) Sebagai Antibakteri Terhadap Escherichia Coli dan Bacillus Cereus Beserta Penentuan Konsentrasi Hambat Minimum (KHM) Alfina Nurrahman; Rahmat Hidayat; Annora Rizky Amalia
Journal of Health, Medical, and Psychological Studies Vol 1 No 2 (2025): December: Sanitas: Journal of Health, Medical, and Psychological Studies
Publisher : CV SCRIPTA INTELEKTUAL MANDIRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.65310/29v9p164

Abstract

This study aimed to evaluate the antibacterial potential of the n-hexane fraction of Keji Besi leaves (Hemigraphis repanda L.) against Escherichia coli and Bacillus cereus, as well as to determine its Minimum Inhibitory Concentration (MIC). Keji Besi leaves were extracted by maceration using 96% ethanol, followed by liquid–liquid fractionation with n-hexane. The antibacterial activity was assessed using the disc diffusion method, while MIC values were determined using the broth dilution method. Phytochemical screening revealed that the n-hexane fraction predominantly contained steroid and triterpenoid compounds. The disc diffusion assay showed that the n-hexane fraction exhibited stronger inhibitory activity against B. cereus than E. coli, with the largest inhibition zone observed at an 80% concentration. MIC determination indicated that the MIC value for B. cereus was 40% (w/v), whereas E. coli required a higher concentration of 80% (w/v) to inhibit bacterial growth. These findings suggest that the n-hexane fraction of Keji Besi leaves demonstrates selective antibacterial activity, particularly against Gram-positive bacteria. Therefore, this fraction has promising potential to be developed as a natural antibacterial agent, especially for infections caused by Gram-positive pathogens.
Evaluation Of Sun Protection Factor Value Of Water, Ethyl Acetate, And N-Hexane Fractions Of Ethanolic Extract Of Crystal Guava Fruit (Psidium Guajava. L.) By UV-VIS Spectrophotometry Farida Kumalasari; Rahmat Hidayat; Tiara Ajeng Listiyani
International Journal of Health Engineering and Technology Vol. 4 No. 6 (2026): IJHET MARCH 2026
Publisher : CV. AFDIFAL MAJU BERKAH

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55227/ijhet.v4i6.661

Abstract

Excessive exposure to ultraviolet (UV) rays can cause various skin damage, so an effective and safe sunscreen preparation is needed. Natural ingredients, especially crystal guava fruit (Psidium guajava L.), are known to contain secondary metabolite compounds such as flavonoids that have the potential as natural photoprotective agents. Crystal Guava Fruit (Psidium guajava L.) contains several secondary metabolite compounds, namely flavonoids, saponins, and terpenoids. The content of secondary metabolites plays a role as a Sun Protection Factor, because these compounds are able to absorb UV A and UV B rays well. This study aims to determine the Sun Protection Factor (SPF) value of ethyl acetate, n-Hexane, and water fractions from crystal guava fruit (Psidium guajava L.) in vitro using the UV-Vis spectrophotometry method and to determine the fraction with the best sunscreen activity. The extract was prepared by maceration using 96% ethanol solvent, then the extract was fractionated in stages using n-Hexane, ethyl acetate, and water. SPF values ​​were determined in vitro using UV-Vis spectrophotometry at a wavelength of 290-320 nm. Each fraction was prepared in several concentrations: 200, 400, 600, 800, and 1000 ppm. The study showed that all fractions had sunscreen activity with SPF values ​​that increased with increasing concentration. The ethyl acetate fraction showed the highest activity with an SPF value of 24.249 at a concentration of 1000 ppm which is included in the ultra protection category. This study shows that the water, ethyl acetate, and n-Hexane fraction of crystal guava fruit (Psidium guajava L.) have the potential as SPF because they are in the range 2-4 (minimum category).
Co-Authors Alfina Nurrahman Amalia, Annora Rizky Farida Kumalasari Tiara Ajeng Listiyani