Journal of Pharmaceutical and Sciences.
Vol 3 No 2 (2020): JPS Volume 3 Nomor 2 (2020)

Isolasi dan Uji Aktivitas Antibakteri Ekstrak Etil Asetat Jamur Endofit Kencur (Kaempferia Galanga L.)

M. Rifqi Efendi (Universitas Dharma Andalas)
Mesa Sukmadani Rusdi (Universitas Dharma Andalas)
Fitria Anisa (Universitas Dharma Andalas)



Article Info

Publish Date
11 Nov 2020

Abstract

The aim of this research was to isolate the endophytic fungi found in leaves and rhizomes of Kaempferia galanga L. and to test its antibacterial activity. Isolation of endophytic fungi is carried out by cultivating and sub-cultivating fungi that found in leaves and rhizomes of Kaempferia galanga L. Identification of endophytic fungi is done by its morphology macroscopically and microscopically. Antibacterial activity testing is carried out by using the agar diffusion method. From the leaves and rhizome of Kaempferia galanga L., five isolates of endophytic fungi were identified as Torulla sp. (KG001), Mucor sp. (KG002), Fusarium sp. (KG003), Geotricum sp. (KG004), and Drechslera sp. (KG005). Three ethyl acetate extracts of the endophytic fungi Torulla sp. (KG001), Fusarium sp. (KG003), and Drechcera sp. (KG005) had antibacterial activity against Gram positive (Staphylococcus aureus and Vibrio cholera) and Gram negative bacterias (Bacillus subtilis and Eschericia coli) at concentration of 3.75%. The ethyl acetate extract endophytic fungi that had strong antibacterial activity (10-20 mm in diameter) were Torulla sp. (KG001) and Drechslera sp. (KG005). Meanwhile, the endophytic fungi that had moderate antibacterial activity (inhibition diameter 5-10 mm) was the ethyl acetate extract Fusarium sp. (KG003).The strongest antibacterial activity was shown by the ethyl acetate extract Drechslera Sp. with an inhibitory diameter 16 mm against Vibrio cholera .

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Journal Info

Abbrev

jps

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health

Description

Journal of Pharmaceutical and Sciences (JPS) with e-ISSN: 2656-3088 is a scholarly peer-reviewed open access journal related to Pharmaceutics, Pharmacology, biotechnology, Pharmacognosy & Phytochemistry, Pharmaceutical Chemistry, Pharmaceutical Biotechnology, Pharmacy practice, Pharmacogenomics, ...