<![CDATA[This study aimed to evaluate the antifungal activity of Piper aduncum secondary metabolites along with additive oils against Sclerotium rolfsii, causing stem rot on patchouli cuttings. It was conducted in two stages—an in vitro experiment using a completely randomized design with seven treatments: P. aduncum oil, combinations of P. aduncum oil with citronella grass, lemongrass, cinnamon leaf, and cloves leaf oils, a synthetic pesticide (propineb 70%), and without essential oils. The second was carried out in vivo using a factorial completely randomized design, in which the first factor was five antifungal formulations, and the second was the spraying interval. The study was done from January to December 2022 at the Laing Solok Experimental Garden, West Sumatra, Indonesia. GC–MS analysis identified four major compounds contained in P. aduncum, citronella, lemongrass, and cinnamon leaf oils, and three in clove leaf oil. All five formulations inhibited fungal growth, with the best combination being P. aduncum + citronella oil, which inhibited growth by 91.85% and biomass by 91.76%. No marked variation with synthetic pesticides as a positive comparison was observed. In the in vivo test, a combination of P. aduncum + citronella and clove leaf oils, which was applied once a week, demonstrated the maximal inhibition, resulting in 0% disease intensity. For a once-fortnight spraying interval, disease intensity increased to 33.3%–100%.]]>
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