The Effect of Pseudomonas aeruginosa Addition on 1,1,1-Trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) Biodegradation by Brown-rot Fungus Fomitopsis pinicola

Atmira Sariwati (Department of Chemistry, Faculty of Science, Institut Ilmu Kesehatan Bhakti Wiyata Kediri, Jl. KH Wahid Hasyim No. 65, Kediri 64114, Indonesia)
Adi Setyo Purnomo (Department of Chemistry, Faculty of Science, Institut Teknologi Sepuluh Nopember, Jl. Kampus ITS Sukolilo, Surabaya 60111, Indonesia)

Indonesian Journal of Chemistry
Vol 18, No 1 (2018)

Effect of addition of Pseudomonas aeruginosa on 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) biodegradation by Fomitopsis pinicola had been investigated. P. aeruginosa was added into F. pinicola culture at 1, 3, 5, 7 and 10 mL (1 mL ≈ 1.53 x 109 P. aeruginosa bacteria cells/mL culture). The addition of 10 mL of P. aeruginosa showed the highest DDT biodegradation approximately 68% during 7 days incubation in Potato Dextrose Broth (PDB) medium, which was higher than biodegradation of DDT by F. pinicola only (42%) at the same incubation time. 1,1-dichloro-2,2-bis(4-chlorophenyl)ethane (DDD), 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene (DDE) and 1-chloro-2,2-bis(4-chlorophenyl)ethylene (DDMU) were detected as metabolites from DDT biodegradation by mixed cultures of F. pinicola and P. aeruginosa.