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All Journal HAYATI Journal of Biosciences Indonesian Journal of Chemistry Jurnal Pharma Bhakta
Atmira Sariwati
Institut Ilmu Kesehatan Bhakti Wiyata Kediri
Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Health Professions Medicine & Pharmacology

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Phytochemical, Antibacterial, and Antioxidant Activities of Anthurium Hookerii leaves Extracts Atmira Sariwati; Inayah Fitri; Adi Setyo Purnomo; Sri Fatmawati
HAYATI Journal of Biosciences Vol. 26 No. 3 (2019): July 2019
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (267.777 KB) | DOI: 10.4308/hjb.26.3.101

Abstract

Many plants of the family of Araceae possess significant benefit as medicinal plants. Anthurium hookerii is herbaceous genus of the family of Araceae. A. hookerii leaves were extracted with five dissimilarity solvents (methanolic, water, ethyl acetate, n-hexane, and dichloromethane). The extracts were evaluated for their phytochemical, total phenolic contents, and antibacterial potential. The presences of tannins and saponins were found in all crude extracts. The steroid was only found in dichloromethane extract, whereas flavonoid was obtained in methanol and water extracts. Besides; methanol, ethyl acetate, water, and n-hexane extracts showed triterpenoid contents. Alkaloid presences in ethyl acetate, methanolic, dichloromethane, and water extracts. The total phenol content was examined by Follin-Ciocalteu assay, which varied from 9.52-76.56 mg/g GAE. The highest total phenolic was found in methanol extract. Antioxidant activity was calculated based on diphenyl picryl hydrazyl radical scavenging ability that showed the scavenging activity with range 7.24-66.11%, which the methanoilic extract have the excellent antioxidant potential (IC50 232.90 µg/ml). Antibacterial activity of leaves extracts of A. hookerii was screened based on disc diffusion method. Water extract showed the wide spectrum antibacterial potential. Klebsiella sp., Bacillus subtilis, Pripioni agnes, and Strepticoccus mutans with maximum diameter of inhibition zone 10.30, 14.20, 9.60, and 15.10 mm, respectively.
The Effect of Pseudomonas aeruginosa Addition on 1,1,1-Trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) Biodegradation by Brown-rot Fungus Fomitopsis pinicola Atmira Sariwati; Adi Setyo Purnomo
Indonesian Journal of Chemistry Vol 18, No 1 (2018)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (357.488 KB) | DOI: 10.22146/ijc.25158

Abstract

Effect of addition of Pseudomonas aeruginosa on 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) biodegradation by Fomitopsis pinicola had been investigated. P. aeruginosa was added into F. pinicola culture at 1, 3, 5, 7 and 10 mL (1 mL ≈ 1.53 x 109 P. aeruginosa bacteria cells/mL culture). The addition of 10 mL of P. aeruginosa showed the highest DDT biodegradation approximately 68% during 7 days incubation in Potato Dextrose Broth (PDB) medium, which was higher than biodegradation of DDT by F. pinicola only (42%) at the same incubation time. 1,1-dichloro-2,2-bis(4-chlorophenyl)ethane (DDD), 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene (DDE) and 1-chloro-2,2-bis(4-chlorophenyl)ethylene (DDMU) were detected as metabolites from DDT biodegradation by mixed cultures of F. pinicola and P. aeruginosa.
Metabolite Bioactive Contents of Parkia timoriana (DC) Merr Seed Extracts in Different Solvent Polarities Venty Suryanti; Atmira Sariwati; Fita Sari; Desi Suci Handayani; Hamdan Dwi Risqi
HAYATI Journal of Biosciences Vol. 29 No. 5 (2022): September 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.5.681-694

Abstract

Parkia roxburghii G.Don seeds have popular folkloric ethnomedicinal use in the treatment of many diseases especially in Indonesia. Methanol, distilled water, n-hexane, and ethyl acetate extracts from the seeds of Parkia roxburghii were assayed for secondary metabolism quantitative, antioxidant, antidiabetic and antibacterial and activities as well as determined the presence of phytochemical constituents. The extracts were investigated for antioxidant possession by DPPH free-radical (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2’ Azinobis (3-ethylbenz-thiazoline-6-sulfonic-acid) scavenging ability, Antidiabetic potential was invitro assayed by α-amylase inhibition and α-glucosyde inhibition while antibacterially by applying the disk diffusion procedure, as well as refined for the attendance of bioavailable phytochemical components. The result showed the existence of phytochemical components in diverse extracts could attribution free scavenging, antidiabetic and antimicrobial activities. The qualitative results all extracts of Parkia roxburghii seeds have expressed the presence of alkaloid, flavonoid, steroid, terpenoids, saponins and tannin whereas, methanol, distilled water, and n-hexane extracts expressed the presence of anthraquinones, The entire phenolic contents were examined as attested by the Follin-Ciocalteu methods, which varied from 43.82 - 137.42 mg GAE/g. The entire flavonoid compounds were measured with aluminum chloride colorimetric procedure, which varied from 20.42 – 45.90 mg QEDW/g. The total phenolic compound were measure Follin Ciocalteu which varied from 43.82-137.42 mg/g. The alkaloid, saponin, tanic acid, terpenoid and cardiac glycoside quantitative were measured with spectrofotometri UV-VIS which varied from 16.34 – 48.90 mg for alkaloid. The Saponin content varied 1.76 – 16.04 mg/g. Tanic acid which varied 0.21 – 7.29 mg/g. Terpenoid which varied 50.12 – 91.02 mg/g. Cardiac glycoside which varied 7.24-36.53 mg/g. The potential antioxidant were measured with ABTS and DPPH method, the methanol extract is the potential antioxidant. Antidiabetic potential were measured with alfa amylase and alfa glucosyde inhibition, the best antidiabetic is methanol extract. The potential antibacterial and antifungal was the methanol extract for Eschericia Coli and Candida Albicans. The conclusion established the tremendous perspective of the Parkia roxburghii seeds as another option fountain of food supplement, as well as drug components.
Effect of the Coconut Coir (Cocos nucifera) as a Growth Medium for Pleurotus ostreatus (Oyster Mushroom) on Mineral and Vitamin B Contents Adi Setyo Purnomo; Atmira Sariwati; Sri Fatmawati; Faradita Eka Puspitasari
HAYATI Journal of Biosciences Vol. 30 No. 1 (2023): January 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.1.95-104

Abstract

The oyster mushroom (Pleurotus ostreatus) is the most cultivated mushroom which its growth performance and nutritional composition depend on substrate types. Thus, this study investigated the effect of coconut coir on minerals and vitamins B contents in the oyster mushroom cultivation medium. The composition of coconut coir and Sengon’s sawdust as growth medium were variated at ratios of 4:0 (F1), 3:1 (F2), 2:2 (F3), 1:3 (F4), 0:4 (F5) (w/w). Furthermore, the mineral and vitamin B contents of the harvested oyster mushrooms were analyzed by ICPMS and LCMS, respectively. The highest minerals contents of potassium 26,909 mg/kg, 1,136 mg/kg of phosphorus, 313 mg/kg of magnesium, 4,346 mg/kg of calcium, 15.4 mg/kg for zinc, 2.07 mg/kg of copper, 0.623 mg/kg of Selenium were identified at F1, whereas the highest manganese was at F2 (15.3 mg/kg). Potassium was the highest mineral content in oyster mushrooms in all growth media, and low toxic minerals content of cadmium was detected between 0.015-0.058 mg/kg in all variant substrates. Lead (0.525 mg/kg) and mercury (0.012 mg/kg) was only detected at F1, while arsenic was detected at F4 (0.002 mg/kg) and F5 (0.029 mg/kg). LCMS analysis showed that vitamin B1 (Thiamine), B3 (niacin), B6 (pyridoxine) and B12 (cyanocobalamin) were detected in all growth media, while vitamin B5 (pantothenic acid) was only detected at F5. These results indicated that the P. ostreatus cultivation on coconut coir become valuable nutritional resources to alleviate malnutrition and help dispose of coconut coir in an environment-friendly manner.
PENETAPAN KADAR BENZOAT PADA SAMPEL MAKANAN SIAP SAJI DAN MIE INSTAN YANG BEREDAR DI WILAYAH KEDIRI Atmira Sariwati
JURNAL PHARMA BHAKTA Vol 1 No 2 (2021): Desember 2021
Publisher : FAKULTAS FARMASI, INSTITUT ILMU KESEHATAN BHAKTI WIYATA

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Latar belakang: Perkembangan industri pengolahan pangan di Indonesia saat ini cukup berkembang dengan cepat seiring dengan pengunaan zat tambahan pangan yang beragam jenisnya. Salah satu yang sering ditambahkan adalah bahan pengawet atau natrium benzoat. Natrium benzoat disebut juga senyawa anti mikroba, tujuan penambahan bahan ini untuk mengurangi tingkat pertumbuhan bakteri, jamur dan khamir pada makanan. Pengunaan natrium benzoat yang berlebihan dapat menimbulkan penyakit lupus (Systemic Lupus Eritematous/SLE), edema (bengkak) akibat dari retensi atau karena naikknya tekanan darah,serta dapat menyebabkan kanker. Tujuan: Mengetahui kadar benzoat pada sampel makanan siap saji dan mie instan yang beredar di Kota Kediri. Metode: Titrasi Asam Basa secara Alkalimetri. Sampel yang di uji adalah pentol bakso, sarden, sosis dan berbagai merk mie yang beredar di kota Kediri. Hasil: Sampel SM 38,88 ppm, IDM 25,92 ppm, SPM 4,94 ppm, SDP 6,28 ppm, SYG 51,84 ppm,CBA 38,88 ppm, BRD 81,22 ppm, PME 29,37 ppm, MGS 58,72 ppm, BHN 27,07 ppm,Sarden 0,08 ppm, Sosis 0,08 pm, dan Pentol bakso 0,12 ppm Simpulan:  Dari 14 sampel yang diuji, sebanyak 3 sampel tidak melebihi ambang batas dan 11 sampel melebihi ambang batas Standar Nasional Indonesia nomor 01-354-1994untuk Natrium benzoat sebesar 1000 mg/kg atau 1 ppm.
Co-Authors Adi Setyo Purnomo Desi Suci Handayani Faradita Eka Puspitasari Hamdan Dwi Risqi Inayah Fitri Sari, Fita Sri Fatmawati Sri Fatmawati Venty Suryanti