The Phenolic Compound from Kalanchoe blossfeldiana (Crassulaceae) Leaf and Its Antiplasmodial Activity against Plasmodium falciparum 3D7

Yenny Febriani Yun (Department of Chemistry, Padjadjaran University, Jl. Bandung-Sumedang Km 21, Jatinangor, Sumedang 45363 Department of Chemistry, Jenderal Achmad Yani University, Jl. Terusan Jendral Sudirman PO BOX 148, Cimahi - West Java)
Faizal Hermanto (Department of Pharmacy, Jenderal Achmad Yani University, Jl. Terusan Jendral Sudirman PO BOX 148, Cimahi - West Java)
Lilis Siti Aisyah (Department of Chemistry, Jenderal Achmad Yani University, Jl. Terusan Jendral Sudirman PO BOX 148, Cimahi - West Java)
Tri Reksa Saputra (Department of Chemistry, Padjadjaran University, Jl. Bandung-Sumedang Km 21, Jatinangor, Sumedang 45363)
Arif Rahman Hakim (Department of Chemistry, Padjadjaran University, Jl. Bandung-Sumedang Km 21, Jatinangor, Sumedang 45363)
Ade Kania Ningsih (Department of Chemistry, Jenderal Achmad Yani University, Jl. Terusan Jendral Sudirman PO BOX 148, Cimahi - West Java)
Tati Herlina (Department of Chemistry, Padjadjaran University, Jl. Bandung-Sumedang Km 21, Jatinangor, Sumedang 45363)
Euis Julaeha (Department of Chemistry, Padjadjaran University, Jl. Bandung-Sumedang Km 21, Jatinangor, Sumedang 45363)
Achmad Zainuddin (Department of Chemistry, Padjadjaran University, Jl. Bandung-Sumedang Km 21, Jatinangor, Sumedang 45363)
Unang Supratman (Department of Chemistry, Padjadjaran University, Jl. Bandung-Sumedang Km 21, Jatinangor, Sumedang 45363)

Indonesian Journal of Chemistry
Vol 16, No 2 (2016)

Various species of Kalanchoe plants have been widely used as raw materials in traditional medicines. This study was the continuation of the researches on secondary metabolites from Indonesia Kalanchoe plants, focused on Kalanchoe blossfeldiana. Fresh K. blossfeldiana leaf was extracted with methanol at room temperature to obtain the concentrated extract. The concentrated methanol extract was dissolved in water and then partitioned successively with n-hexane and ethyl acetate. The methanol, n-hexane, ethyl acetate extracts were tested using antiplasmodial assay against Plasmodium falciparum 3D7. The IC50 of methanol, n-hexane, and ethyl acetate extract were 13.002; 2.807, and 11 nM, respectively. Ethyl acetate extract was separated by the combination of chromatography on silica and ODS. This process produced the yellow solid. The chemical structure of the compound was determined based on UV, IR, MS, 1H-NMR, and 13C-NMR analyses and the comparison of data obtained from the literature and identified as phenolic compound, namely 3,3',4',5,7-pentahydroxyflavone or quercetin (1), and displayed antiplasmodial activity with IC50 3.97x10-2 nM.