Effect of bone marrow mesenchymal stem cells transplantation on BMP-15 expression and Graafian follicle count in mice model of endometriosis

Ratriana Via Parasti (Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Airlangga, Dr Soetomo Hospital, Surabaya)
Widjiati Widjiati (Department of Veterinary Obstetrics, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya)
Sri Ratna Dwiningsih (Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Airlangga, Dr Soetomo Hospital, Surabaya)

Majalah Obstetri dan Ginekologi
Vol. 26 No. 3 (2018): December

Objectives: To determine the effect of bone marrow mesenchy-mal stem cells (BMSCs) on BMP-15 expression and Graafian follicle count in endometriosis mice.Material and Methods: This study was a laboratory randomized clinical trial on Mus musculus. The object of the study was 42 mice which were divided into 3 groups, the control, endome-triosis, and endometriosis + BMSCs groups. Comparison of BMP-15 expression and Graafian follicle count between groups was evaluated.Results: Immunohistochemical analysis showed that BMP-15 expression in control, endometriosis, and endometriosis + BMSCs groups had p=0.551, p=0.446 and p=0.917 with ANOVA test p=0.273, indicating no statistically significant differences between groups . Graafian follicular count in the three groups had p=0.31, p=0.001, and p=0.006, with the Kruskal-Wallis test p=0.001. Graafian follicles in the endometriosis + BMSCs group were higher than those in control and endometriosis groups.Conclusion:In the endometriosis mouse model with bone mar-row stem cell transplantation the BMP-15 expression in each group did not show a difference, but a significant difference was found in the number of Graafian follicles.