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All Journal Saintika Medika: Jurnal Ilmu Kesehatan dan Kedokteran Keluarga. Folia Medica Indonesiana Indonesian Journal of Cancer Universa Medicina Journal Of Vocational Health Studies Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) Jurnal Medik Veteriner journal of Basic Medical Veterinary Current Biomedicine

Widjiati Widjiati, Widjiati

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Author-ID : 596972

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary Other

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Red fruit oil increases trophoblast cells and decreases caspase-9 expression in placenta of lead exposed mice Sumarsono, Portia; Widjiati, Widjiati; Susilowati, Suherni
Universa Medicina Vol 35, No 2 (2016)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2016.v35.110-118

BACKGROUNDLead is able to pass through the placental barrier and interfere with fetal development. Red fruit has high antioxidant activity, due to carotenoids (pro-vitamin A), tocopherols (vitamin E) and unsaturated fatty acids. This study aims to examine the effect of red fruit oil towards placenta in pregnant mice before lead exposure.METHODSThis was a laboratory experimental post test only study, using 20 pregnant mice (Mus musculus) that were randomized into five groups. The negative control group was not exposed to lead; intervention group 0 (P0) was exposed to lead at 0.011 mg/20 g BW/day on days 6-15 of gestation. The intervention groups P1-P3 were given red fruit orally at 0.3 mL/20 g BW/day, 0.8 mL/20 g BW/day, 0.9 mL/20 g BW/day, respectively, before lead exposure. Normal trophoblast cell count and caspase-9 expression of trophoblasts were calculated. One-way ANOVA, Kruskal-Wallis and Mann Whitney tests were used to analyze the data.RESULTSThe intervention groups P2 and P3 given red fruit at 0.8 mL/20 g BW/day and 0.9 mL/20 g BW/day showed a significant increase in normal trophoblast cell count of 88.2% (p=0.024) and a decrease in caspase-9 expression of 70.8% compared with P0 to which was not given red fruit (p=0.020). CONCLUSIONRed fruit increased normal trophoblast cell count by 88.2% and decreased caspase-9 expression by 70.8% in pregnant mice before lead exposure. It should be noted that red fuit may prevent oxidative damage to cells.

Effects of Curcumin on Vascular Endothelial Growth Factor Expression on Rattus norvegicus Cervical Cancer Xenograft Model Muninggar, Laili; Widjiati, Widjiati; Yuliati, Indra; Askandar, Brahmana; Hartono, Poedjo
Indonesian Journal of Cancer Vol 12, No 3 (2018): July-September
Publisher : National Cancer Center - Dharmais Cancer Hospital

Objective: To analyze the effect of curcumin in VEGF expression on Rattus norvegicus cervical cancer cell xenograft model.Methods: An experimental study with randomized post test only control group design. The subjects were Rattus norvegicus (Sprague Dawley), inoculated with He-la cervical cancer cells from American Type Culture Collection (ATCC) processed in stem cell laboratory Institute of Tropical Disease (ITD) Airlangga University. 5x106 of He-La cells were injected subcutaneously in dorsal flank area of Rattus norvegicus. After 30 days of observation we performed histopathological examination of xenograft tissue and randomized into 2 groups which were given curcumin orally 1000 mg/kg (curcumin group) vs. no therapy (control group). After another 30 days the xenograft tissue was dissected and underwent immunochemistry examination for VEGF expression.Results: 32 samples of Rattus norvegicus were divided into 2 groups, In curcumin group the VEGF median expression was 2,2 (0,3-7,6) and in control group the VEGF median expression was 6,6 (1,2-12). There was a statistically significant difference with p value =0,009 with Mann Whitney test (p<0,05).Conclusion: VEGF expression in Rattus norvegicus xenograft model of cervical cancer was suppressed by giving Curcumin 1000 mg/kgBB orally.

MOZART MUSIC INCREASES THE NUMBER OF GLIAL CELLS COMPARED TO INDONESIA POP AND RELIGIOUS MUSIC Rahayu, Pipit Sri Estuning; Hermanto, Hermanto; Widjiati, Widjiati
Saintika Medika: Jurnal Ilmu Kesehatan dan Kedokteran Keluarga Vol 15, No 2 (2019): December 2019
Publisher : Universitas Muhammadiyah Malang

Music stimulation is an important component for prenatal fetal development. Both pop and religious music are easy to listen and widely accepted in Indonesia. This study was to analyze the effect of Mozart, pop, and religious music exposure during pregnancy to the number of glial cells in the brain of Rattus norvegicus offspring. The samples were divided into three groups based on the exposure for each group, namely Mozart, pop, and religious music, duration of 60 minutes with 65dB intensity, initiated on the 10th day of pregnancy for 9 days in the soundproof chamber. Three brains of the offsprings were dissected and prepared for Hematoxylin-Eosin staining counted on 5 fields of view and 400 magnification strength.Different glial cells number of Rattus norvegicus brain between groups were observed. Mozart music (28,29) showed a highest mean and pop music (18,67) showed the lowest mean. Significant difference of the number of brain glial cells between Mozart music compared to pop and religious music groups were observed, with p value <0,005.The number of brain glial cells of Rattus norvegicus offsprings in the Mozart group were significantly higher than those in pop and religious groups.

Synapsis Expression Increased By Mozartâ€™s Music Stimulation Compared With Indonesian Pop And Religious Music Amila, Ukhti Mukminah ilmi; Joewono, Hermanto Tri; Widjiati, Widjiati
Saintika Medika: Jurnal Ilmu Kesehatan dan Kedokteran Keluarga Vol 16, No 1 (2020): June 2020
Publisher : Universitas Muhammadiyah Malang

Classical music stimulation is a proven method that affect intelligence as well as brain function. Mozartâ€™s classical music is classical music that could stimulate the growth of synaptic networks. To analyze the difference of synapsin expression of the brain of Rattus norvegicusÂ offspring on Mozartâ€™s, pop, religious and without music stimulation during pregnancy. Experimental. Treatments were subjected to Rattus norvegicusas a subject organism, initiated on the 10th day of pregnancy, in 1 hour duration in a soundproof chamber, intensity of 65 dB. On the 19th day of pregnancy the subjects were sacrificed and three offsprings were chosen. The head of the chosen Rattus norvegicusoffsprings were decapitated and the brain subsequently sectioned to calculate synapsin expression by immunohistochemistry method with 400 magnification strength microscope on the 5 field of view. Significant difference of synapsin expression of the brain of Rattus norvegicus offspring was observed between Mozartâ€™s and control group. Significant differences of synapsin expressions were observed in the cerebellum of the newly born Rattus novergicus between Mozart and pop music groups (p = 0,007), Mozart and religious music groups (p = 0,008), Mozart and without music groups (p = 0,004).Synapsin expression of Rattus norvegicus brain offsprings were higher in Mozart music stimulation compared with pop and religious music stimulation

Hubungan teknik pemerahan dengan jumlah Escherichia coli pada susu segar dari peternakan sapi perah di KUD Kertajaya, Kabupaten Kediri, Jawa Timur Rahadyan, Raihan Alif; Tyasningsih, Wiwiek; Puspitasari, Yulianna; Permatasari, Dian Ayu; Widjiati, Widjiati
Current Biomedicine Vol. 1 No. 1 (2023): January
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.1.1.19-24

Background: The coliform bacteria group is a group of bacteria used as an indicator of food sanitation, one of which is in dairy products. Objective: This study aimed to determine the relationship of milking techniques with the number of Escherichia coli in raw milk compared to the bacterial contamination limit set by the National Standardization Agency. Methods: The design of this study was a descriptive study. Raw milk samples were taken from Lucky Farm and several traditional dairy farms in the KUD Kertajaya area, Kediri Regency, East Java. This study uses the most probable number (MPN) test. Results: The results showed 11 (73.3%) of raw milk samples using conventional milking techniques exceeding the maximum limit of E. coli bacterial contamination determined by the National Standardization Agency (< 3 MPN/mL). However, all 15 samples, or 100% of raw milk samples using machine milking techniques, were negative for E. coli and met the maximum bacterial contamination limit requirements. Conclusion: The milking techniques affect E. coli contamination limits in raw milk. The milk samples taken using machine milking techniques had better results with MPN values < 3 MPN/mL than those taken using conventional milking techniques.

Pengaruh Paparan Uap Rokok Elektrik Terhadap Gambaran Histopatologi Organ Pulmo Tikus Putih (Rattus norvegicus) Ardy, Carolina; Hamid, Iwan Sahrial; Yudhana, Aditya; Widjiati, Widjiati
Jurnal Medik Veteriner Vol. 3 No. 1 (2020): April
Publisher : Universitas Airlangga

Studi ini bertujuan untuk mengidentifikasi pengaruh paparan uap rokok elektrik terhadap gambaran histopatologi organ pulmo. Tikus putih yang digunakan sebanyak 20 ekor yang terbagi menjadi 5 kelompok, kontrol (K) adalah yang tidak diberikan paparan uap rokok elektrik dan kelompok perlakuan yang diberikan paparan uap dengan dosis yang berbeda, kelompok Perlakuan I (0,3 mg/ml), P2 (3 mg/ml), P3 (12 mg/ml), P4 (36 mg/ml). Setelah 14 hari perlakuan, tikus putih dieuthanasia dan dinekropsi serta organ pulmo diawetkan untuk dilakukan pembuatan preparat histopatologi dengan menggunakan pewarnaan Hematoxilin-Eosin. Hasil menunjukkan terdapat kerusakan pada gambaran histopatologi pulmo yang diamati pada perbesaran 400x. analisis data menggunakan Kruskal-Wallis dan dilanjutkan dengan uji Mann Whitney menunjukkan perbedaan yang signifikan (p<0.05). Rata-rata kerusakan organ pulmo tikus putih adalah kontrol (K)=3.00; (P1)=6.00; (P2)=12.00; (P3)=13.00 dan (P4)= 18.50 dan dosis minimal dari nikotin adalah 3 mg/ml. Kesimpulan, paparan uap rokok elektrik dapat menyebabkan kerusakan yang signifikan pada organ pulmo tikus putih.

Protective Effects of Apis Dorsata Honey Supplementation on Kidney Histopathology in Mice with Monosodium Glutamate Exposure Rao, Sherbrina Bai Seenivasa; Widjiati, Widjiati; Lokapirnasari, Widya Paramita; Triakoso, Nusdianto; Safitri, Erma; Kuncorojakti, Suryo; Proboningrat, Annise
Jurnal Medik Veteriner Vol. 6 No. 3 (2023): Special Issue: International Conference on Veterinary Medicine and Health Scien
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss3.2023.63-69

This study was conducted to investigate the protective effects of Apis dorsata honey on the histopathological changes of the kidney in mice exposed to monosodium glutamate. This study used 25 male mice as the experimental animal which were divided into five groups with five replication, respectively i.e. (C-) was administered basal diet, (C+) was administered 4 mg/g BW MSG, (T1) was administered A. dorsata honey of 53.82 mg/20g BW followed by MSG 4 mg/g BW, (T2) was administered A. dorsata honey of 107.64 mg/20g BW followed by MSG 4 mg/g BW, and (T3) was administered A. dorsata honey of 161.46 g/20g BW followed by MSG 4 mg/g BW. All treatments were carried out orally for 52 days. This study was observed tubular epithelial hydropic degeneration, epithelial necrosis, and glomerular necrosis. Data were analyzed using Kruskal-Wallis test followed by the Mann-Whitney test. As a result, for the epithelial and glomerular necrosis variables, the T3 group showed significant results when compared with C+, T1, and T2 groups. Moreover, T3 was not significantly different from the C- group. It can be concluded that A. dorsata honey in T3 group could protect mice kidneys from the damaging effect of MSG.

POTENSI KANDUNGAN ANTIOKSIDAN RUMPUT KEBAR (Biophytum Petersianum Klotzsch) TERHADAP APOPTOSIS DAN GAMBARAN HISTOPATOLOGIS TESTIS PADA MENCIT (MUS MUSCULUS) YANG DIPAPAR 2,3,7,8-Tetrachlorodibenzo-p-dioxin Dewita, Dewita; Widjiati, Widjiati; Rimayanti, Rimayanti; Suprayogi, Tri Wahyu; Hariadi, Mas'ud; Plumeriastuti, Hani
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19986

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is the most toxic compound in the dioxin group. This compound is a pollutant for the environment and very harmful to human health and enter the body through the mucous membranes in the mouth and the respiratory tract and can be transmitted through the placenta and lactation. The aim of this study was to know the antioxidantpotency of to resolve reproduction disturbance caused by TCDD exposure.Thirty Balb/C male mice were divided into five different groups, the negative control group, a positive control group exposed to TCDD at a dose of 7 Î¼g/kg BW, P1 group of groups exposed to TCDD doses of 7 Î¼g / kg BW and given Biophytum petersianum extract 0.05mg/gBB/day, group P2 group exposed to TCDD doses of 7Î¼g/kgBW and given 0.080mg/gBB/day, and group P3 were exposed to TCDD dose of 7Î¼g/kgBB and given the extract of Biophytum petersianum 0.135mg/gBB/day during day 2 to day 55. On the 56th day the mice were sacrificed and apoptotic examination and spermatogenic cell histopathological features were performed on the testis. The results showed that: P2 (0,433 ± 0,497; p<0,05) and P3 (0,200 ± 0,000 p<0,05) groups were the most effective group in decreasing spermatogenic cell apoptosis compared to positive control group (2,933 ± 1,5832 p<0,05). The Johnsen score result showed that P2 (9,400 ± 0,420) and P3 (9,800 ± 0,253) groups improving the histopathologic picture of spermatogenic cells in seminiferous tubules compared to positive control group (7,20 ± 0,400) p<0,05. Conclusion of this study were Biophytum petersianum is effective to solve reproduction disturbances caused by exposure of TCDD and the P3 group is the most effective group.

DESCRIPTION OF HEMATOLOGICAL VALUE IN LEOPARD GECKO (Eublepharis macularius) Wiranata, Try Ardhi; Widjiati, Widjiati; Wahyuni, Retno Sri; Hidajati, Nove; Poetranto, Emmanuel Djoko; Yuliani, M. Gandul Atik
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19990

The aim of this research was to explore the hematological value of leopard gecko (Eublepharis macularius). Total of 20 samples were examined, its consists of 10 males and 10 females, blood samples were taken from vena ventral abdominal and deposited into EDTA tubes, fresh blood leaves little to make blood smear, total blood examination conducted with automatic hematological analyzer ABX MICROS 60 and for total leukocyte and differential leukocyte count used standard manual method. The mean result of hematological value of leopard gecko in this research are WBC (x103/mm3) 7,12 male, 8,09 female, Lymphocyte (%) 46,2 male, 38,4 female, Monocyte (%) 17 male, 17,3 female, Heterophil (%) 18,3 male, 21,8 female, Eosinophil (%) 10,8 male, 13,9 female, Basofil (%) 7,7 male, 8,6 female, RBC (106/mm3) 0,64 male, 0,65 female, HGB (g/dl) 8,95 male, 9,46 female, Hematocrit (%) 10,55 male and 9,26 female. In all parameters showed that hematology value of male gecko leopard was lower than female gecko except on two parameters which are hematocrit and lymphocyte which showed male gecko has the higher value than females, but the hematology value difference of both sexes leopard gecko is not significant except in heterophil value.

PENGARUH PEMBERIAN EKSTRAK RUMPUT KEBAR (Biophytum petersianum Klotzsch) TERHADAP JUMLAH SEL SERTOLI MENCIT (Mus musculus) YANG DIPAPAR 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN Berlina, Cyrcilia Relita; Eliyani, Hana; Sami, Abdul; Widjiati, Widjiati; Mulyati, Sri; Anwar, Chairul
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19991

This study was aimed to know the effect of Kebar grass extract on amount of mice Sertoli cell exposed by TCDD. This study was experimental study with completely randomized design. Thirty adult male mice of Mus musculus strain Balb/C, age 11 weeks and weight 25 - 30 ~were used. Mice were divided into five groups, that were: K- was control (aquadest 0) mL); K+ was injected with TCDD at dose of 7 µg/kg BW; Pl was injected with TCCD and administrated with Kebar extract (0,045 mg/ g BW/day); P2 was injected with TCCD and administrated with Kebar extract (0,080 mg/ g BW/day); P3 was injected with TCDD and administrated with Kebar extract (0,135 mg/ g BW/day). Kebar grass extract was administrated in 53 days. Mice were sacrificed and right testis organs were taken. Then, histology preparat with HE staining were made and Sertoli cells were counted. Data were analyzed by One Way ANOV A followed by Duncan test (a = 0,05). The result of this study showed that administration of Kebar grass extract affected amount of mice sertoli cells. K+ (2,92a±0,109) showed significance difference (p<0,05) compared to Pl (5,00b±0,374), P2 (7,64c±0,409) and P3 (9,68ct±0,363). Meanwhile, amount of Sertoli cells of P3 at the highest dose did not show significance difference (p>0,05) with K- (10,16<l±0,829). The conclusion of this study was administration of Kebar grass extract per oral in 53 days could maintain amount of mice Sertoli cell exposed by TCDD.

Co-Authors Achmad Salman Faridz, Achmad Salman Agung Budianto Achmad, Agung Budianto Amila, Ukhti Mukminah ilmi Annise Proboningrat Ardy, Carolina Arimbi Askandar Tjokroprawiro Bambang Poernomo Berlina, Cyrcilia Relita Budi Wicaksono Chairul Anwar Dewita Dewita Eighty Mardiyan K, Eighty Eka Pramyrtha Hestianah, Eka Pramyrtha Erma Safitri Erry Gumilar Dachlan, Erry Gumilar Gandul Atik Yuliani Hana Eliyani Hani Plumeriastuti Hariadi, Mas'ud Hermanto Hermanto Hermanto Tri Joewono Hidajati, Nove Hidayatullah, Amiruddin IMAM MUSTOFA INDRA YULIATI, INDRA Iwan Sahrial Hamid Jeanita, Winny Jojor Sihotang Kuncorojakti, Suryo Lilik Maslachah Luqman, Epy Muhammad Mafruchati, Maslichah Meles, Dewa Ketut Muninggar, Laili Ngakan Made Rai Widjaja Nurbayatin, Anita Nursadida, Milla Nusdianto Triakoso Pardede, Sabrina Melisa Pardede, Sabrina Melisa Paulus Sugianto, Paulus Permatasari, Dian Ayu Poedjo Hartono Poetranto, Emmanuel Djoko Portia Sumarsono, Portia Rahadyan, Raihan Alif Rahayu, Pipit Sri Estuning Rahmaniabdi, El Roy Achmad Rao, Sherbrina Bai Seenivasa Relly Yanuari Primariawan, Relly Yanuari Rimayanti Rimayanti Rochmah Kurnijasanti Rusyawardani, Aldis Ingrid Sami, Abdul Shabira, Zahra Sri Mulyati Sri Pantja Madyawati, Sri Pantja Suherni Susilowati Sulistiawati Sulistiawati Suzanita Utama Tita Damayanti Lestari Tri Wahyu Suprayogi Tyasningsih, Wiwiek Viski Fitri Hendrawan Wahyuni, Retno Sri Widya Paramita Lokapirnasari Wiranata, Try Ardhi Yeni Dhamayanti Yudhana, Aditya Yudiwati, Rina Yulianna Puspitasari

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