Penetapan Kadar Alkaloid Ekstrak dari Etanolik Bunga Kembang Sepatu (Hibiscus rosa-sinensis L.)

Murrukmihadi, Mimiek ( Fakultas Farmasi Universitas Gadjah Mada Yogyakarta)
Wahyuono, Subagus ( Fakultas Farmasi Universitas Gadjah Mada Yogyakarta)
Marchaban, . ( Fakultas Farmasi Universitas Gadjah Mada Yogyakarta)
Martono, Sudibyo ( Fakultas Farmasi Universitas Gadjah Mada Yogyakarta)

JFIOnline
Vol 6, No 3 (2013)

Kembang sepatu flower (Hibiscus rosa-sinensis L.) was fractionally used as expectorant. Based on Bioassay Guided fractionation, an active fraction was separated, and the fraction was identified is Alkaloid was the major compound based on TLC analysis. Viscosity value measured by viscometer was used as a Bioassay model of expectorant activity in vitro and acetyl cysteine was used as positive control. Alkaloid content determination of the ethanolic extract was measured by TLC-Densitometric compared with standard curve of isolated alkaloid as the selected marker (Y=12,1360X+2901,4474). The alkaloid content in the ethanolic extract was determined as 2.35±0,67%.Keywords : alkaloid, ethanolic extract, Hibiscus rosa-sinensis L.