Garuda - Garba Rujukan Digital

Article Per Year (5 Year)

All

Jurnal Teknologi Laboratorium

Website

Published by Poltekkes Kemenkes Yogyakarta

ISSN : - EISSN : - DOI : -

Core Subject : Science, Education,

Chemistry Other

Arjuna Subject : -

Articles 6 Documents

Search results for , issue "Vol 6 No 2 (2017): 2017 (2)" : 6 Documents clear

Pemanfaatan Ekstrak Daun Jambu Biji (Psidium guajava Linn) Sebagai Antibakteri dan Antifungi Nuryani, Siti
Jurnal Teknologi Laboratorium Vol 6 No 2 (2017): 2017 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Guava (Psidium guajava Linn) is found throughout Indonesia. The leaves contain tannins that can be used as antibacterial and antifungal. The aims of this research are to determine the antiseptic power of guava leaf as antifungal and antibacterial.This research is pre experiments research with laboratory test to determine the inhibitory power of guava ethanol extract as anti-bacterial and anti-fungal. Guava leaves are old made 70% ethanol extract in LPPT UGM using maceration method. The extract made 3 concentrations ie 25%, 50% and 75%. Each concentration was tested for inhibitory by knowing the diameter of growth barrier to Candida albicans and Staphylococcus aureus. Each concentration is repeated 5 times, resulting in 15 data. The data were analyzed descriptively to illustrate their potential comparisons with chlorhexidine as standard materials. The extract of guava leaf using ethanol 70% in laboratory test resulted in average inhibitory zone diameter as follows: for mushroom C.albicans with extract 25%, 50%, and 75% were 13.4mm, 17.6mm, and 19.4mm. While for S. aureus is 2.2mm, 25.6mm, and 27.2mm. The effect of antifungal power of guava leaf extract (Psidium guajava Linn.) on the growth of Candida albicans fungus is smaller compared to Staphylococcus aureus bacteria

Efektivitas Variasi Garam Salmiak (NH4Cl) Dan Sentrifugasi Pada Pemeriksaan Basil Tahan Asam Penderita Tuberculosis Siregar, Saadah; Supriatin, Yati; Noor, Lanny
Jurnal Teknologi Laboratorium Vol 6 No 2 (2017): 2017 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

According to a report by WHO Indonesia, it is a case number three infected with tuberculosis. Tuberculosis disease is caused by Mycobacterium tuberculosis that belongs to acid-fast bacilli bacteria (BTA). Sputum smear examination microscopically still has many deficiencies caused by mucoprotein in sputum so that BTA cannot be separated from sputum, this is causing a false negative in reading result Microscope. The objective of this research is to know the most effective variation of concentration, speed and time of centrifugation on smear examination in tuberculosis patients. The research method used is experimental method and Design of complete Random Block design. Data were analyzed by statistic test with ANOVA test on SPSS. The result of the research is that at concentration 2% NH4Cl It can increase the number of BTA with optimal centrifugation speed / effective at 5000 rpm for 15 minutes. Suggestion for further research is to use salmaniac salt concentration with a concentration lower than 2%.

Identifikasi Gen Ctx-M Pada Esherichia Coli Penghasil Extended Spectrum Beta-Lactamases (Esbls) Di RSUD Dr. Soetomo surabaya Prasetya, Yulianto Ade
Jurnal Teknologi Laboratorium Vol 6 No 2 (2017): 2017 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Escherichia coli producing Extended-Spectrum Beta-Lactamases (ESBLs) responsible for the high number of disease and death from nosocomial infections because of an enzyme encoded by genes CTX-M. This aim of the research to identify isolates E.coli of urine patients, which is a collection of Clinical Microbiology RSUD Dr. Soetomo Surabaya on the January-February 2014. The kind of the research use is observational descriptive with molecule approachment. The sample used in clinical isolate of E.coli producing ESBLs accumulated during January-February 2014 collection Clinical Laboratory RSUD Dr. Soetomo Surabaya that is thirty isolate. The methods used for the detection of genotypic by PCR thus electrophoresis and visualized on agarose gel 1.5%. The results show that twenty-seven isolate (90%) positive containing a gen CTX-M with the highest number found on the Interna Departement. Detection Bacteria producing ESBLs in genotype important that therapy an antibiotic the patients are given more effective and efficient.

CEMARAN Staphylococcus aureus DAN Pseudomonas aerogenosa PADA STETESKOP DIRUMAH SAKIT lutpiatina, leka
Jurnal Teknologi Laboratorium Vol 6 No 2 (2017): 2017 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Medical devices that are susceptible to bacteria contaminated one of them is a stethoscope. Nosocomial infections that are problematic in the hospital include Staphylococcus aureus and Pseudomonas aerogenosa. These bacteria can cause mild skin infections to serious life-threatening infections. The purpose of this research is to know the existence of bacteria contamination of Staphylococcus aureus and Pseudomonas aerogenosa on steteskop at Banjarbaru hospital. This research uses descriptive survey method, which is a research method to describe objectively based on facts found. The samples were stethoscope in child care room, internal medicine room, delivery room, post partum room, perinatology room, and post-surgical treatment room at Banjarbaru hospital for 30 stethoscopes.The result of the research was found contamination of Staphylococcus aureus and Pseudomonas aerogenosa on steteskop. The site home condition of the research data was 66.7% cleaned daily, the storage method was placed on the table 70% and the duration of using the set home more than 1 year as much as 70%. The conclusion of stethoscope at Banjarbaru Hospital was contaminated with Staphylococcus aureus by 70% and Pseudomonas aerogenosa by 17%. The suggestion of research can be continued by knowing the existence of Staphylococcus aureus resistant antibiotic and Pseudomonas aerogenous antibiotic resistant at steteskop at Hospital.

Identifikasi Candida Albican Pada Saliva Wanita Penderita Diabetes Melitus Farizal, Jon; Abdul Rahman Serbasa Dewa, Exchagusesa
Jurnal Teknologi Laboratorium Vol 6 No 2 (2017): 2017 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Diabetes mellitus is a predisposing factor for the onset of oral candidiasis. Women with diabetes have extra sugar in the secretion of saliva. Sugar contained in saliva are stacked on the mucosa so provide food for mold growth so that the diabetic patient saliva will be found Candida albicans The aim of this study was to identify Candida albicans in saliva of women with diabetes mellitus in patient wards of Medicine Hospital Dr M.Yunus Bengkulu 2017.This type of research is conducted by using descriptive survey design. Sampling technique using Purposive Sampling, the number of samples identified as many as 27, conducted by macroscopic and microscopic examination. Data in this research using descriptive analysis.Results of univariate analysis obtained, all respondents the majority of respondents 52% positive saliva fungus Candida albicans and almost half of respondents 48% negative saliva fungus Candida albicans. The prevalence values indicate that the majority of the total population of women with DM saliva samples contained fungus the Candida albicans. Whole saliva respondents (100%) in women with diabetes is growing colonies on Media SDA (Saboraud Dextrose Agar) fungus Candida albicans

Pengembangan Mikroskop Dengan Mikrokontroler dan Cahaya Monokromatik Untuk Mendeteksi Parasit Malaria Susanti, Ida; Handayani, Sarwo; Ekowatiningsih, Riyanti; Prasetyorini, Budi; A Yusnita, Endah; Ardianto, Donni Agus; K Widjaya, Sastra
Jurnal Teknologi Laboratorium Vol 6 No 2 (2017): 2017 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Malaria still become one of major health burden in Indonesia especially in remote areas of east Indonesia. Golden standard of malaria parasite detection is still microscopic technique using polychromatic light source whether from halogen or natural light source. A microscopic technique has a lot of benefits but still have weaknesses, such as time-consuming and bias on the reading by microscopist, because of artifact in the image. Aims of this study were to designed malaria parasites detection tool that is robust, fast, convenient and clear by minimizing artifact on the slide. Design of this study was laboratory experimental which modified simple microscope into an automatic microscope with table movement and webcam recording using a microcontroller and monochromatic light source. The wavelength of the light sources was 402nm(blue), 532 nm (green) and 650 nm (red), the intensity of each source differed. The reading of the slide image was conducted by two certified microscopists, who read 60 images of a thick and thin slide with three different live stage of Plasmodium falciparum live, which wearing, trophozoite and schizont. This study showed that modification of microscope was succeeded with automatic movement and webcam recording, process time in one step movement and recording approximately 10 seconds or 17minutes for 100 fields of view as confirmation process. The monochromatic light source has proven to give a clear and contrast field of view when the intensities were higher than 40 mW and the certified microscopist able to identified Plasmodium falciparum parasites. Data analysis of microscopist reading used nonparametric statistic Friedman by SPSS showed that correlation between images using monochromatic and polychromatic lights have meaningless differences in a thick and thin slide. However, hemozoin as a marker of Plasmodium falciparum parasite was less detected by monochromatic light used in this study.

Page 1 of 1 | Total Record : 6

Filter by Year

2017 2017

Filter By Issues

All Issue Vol 10 No 1 (2021): inpress Vol 9 No 1 (2020): 2020 (1): Special Edition "COVID-19" Vol 9 No 2 (2020): 2020 (2) Vol 9 No 1 (2020): 2020 (1) (inpress) Vol 9 No 1 (2020): 2020 (1) Vol 9 No 2 (2020): (In Progress) Vol 8 No 2 (2019): 2019 (2) Vol 8 No 1 (2019): 2019 (1) Vol 7 No 2 (2018): 2018 (2) Vol 7 No 1 (2018): 2018 (1) Vol 6 No 2 (2017): 2017 (2) Vol 6 No 1 (2017): 2017 (1) Vol 5 No 2 (2016): 2016 (2) Vol 5 No 1 (2016): 2016 (1) Vol 3 No 2 (2014): 2014 (2) Vol 3 No 1 (2014): 2014 (1) More Issue

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name -

Contact Email -

Phone -

Journal Mail Official -

Editorial Address -

Location Kota yogyakarta, Daerah istimewa yogyakarta INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA