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Jurnal Bios Logos
Published by Universitas Sam Ratulangi
ISSN : 20889569     EISSN : 26563282     DOI : https://doi.org/10.35799/jbl.11.2.2020.28153
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
JURNAL BIOS LOGOS is the journal published by Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University. The aims of the journal are to publish original research papers and article review in biology science i.e. botany, zoology, molecular biology, microbiology, ecology, diversity and conservation, taxonomy and biogeography. BIOS LOGOS is published two times per year (February and August)
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 5 Documents
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Search results for , issue "Vol 5, No 1 (2015): JURNAL BIOSLOGOS" : 5 Documents clear
Fungi Tanah Perkebunan Kopi dan Potensinya sebagai Agen Antagonis (The Soil Fungi of The Coffee Plantation And Its Potential as Antagonistic Agent) Samingan, Samingan
JURNAL BIOS LOGOS Vol 5, No 1 (2015): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.5.1.2015.9318

Abstract

Abstrak Fungi tanah dari perkebunan kopi telah diisolasi untuk mendapatkan fungi antagonistik sebagai pengendali hayati untuk patogen busuk akar kopi. Sampel diambil dari 3 kecamatan di Kabupaten Bener Meriah Provinsi Aceh. Isolasi fungi dilakukan dengan metode pengenceran. Hasil penelitian menunjukkan bahwa fungi yang diisolasi dari perkebunan kopi Kabupaten Bener Meriah tidak menunjukkan adanya perbedaan indeks keragaman dan kemerataan. Ditemukan tiga kelompok fungi yang dominan ditemukan di 3 lokasi pengambilan sampel, yaitu Aspergillus, Penicillium dan Trichoderma. Hasil uji antagonistik menunjukkan bahwa Trichoderma sp2 memiliki kemampuan antagonistik yang lebih tinggi terhadap fungi patogen busuk akar kopi. Kata-kata kunci: fungi antagonis, fungi patogen, fungi tanah, busuk akar kopi   Abstract Soil fungi of the coffee plantation was isolated to find the antagonistic fungi as biocontrol agent for pathogenic fungi of coffee root rot. Samples were obtained from three subdistrict of Bener Meriah Regency in Aceh Province. Fungi isolates were obtained by the dilution method. The results showed that the fungi isolates from coffee plantation in Bener Meriah Regency were not different in diversity and evenness indices. There were three groups of dominant fungi in these 3 sampling locations, i.e. Aspergillus, Penicillium and Trichoderma. Based on antagonistic test, Trichoderma sp2 was able to inhibit the pathogenic fungi of coffee root rot. Key words: antagonistic fungi, pathogenic fungi, soil fungi, coffee root rot
Kepadatan Cacing Tanah pada Lahan Pertanian Tomat Terpapar Pestisida di Desa Ampreng, Kecamatan Langowan Barat - Provinsi Sulawesi Utara (Earthworm Density in Tomato Farming Exposed to Pesticides at Ampreng Village, Langowan Barat Sub District – North S Tribrata, Yulita; Siahaan, Ratna; Pelealu, Johanis J; Mambu, Susan M
JURNAL BIOS LOGOS Vol 5, No 1 (2015): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.5.1.2015.7045

Abstract

Abstrak Penggunaan pestisida dimaksudkan untuk mempertahankan produksi pertnaian tomat namun hal ini dapat mempengaruhi kepadatan cacing tanah. Penelitan bertujuan menganalisis kepadatan cacing tanah pada lahan pertanian tomat terpapar pestisida di Desa Ampreng, Kecamatan Langowan Barat – Provinsi Sulawesi Utara. Hasil menunjukkan bahwa di lahan pertanian ada dua jenis cacing tanah (Pontoscolex sp. dan Amynthas sp.) dan di lahan pekarangan ada empat jenis (Pontoscolex sp., Amynthas sp., Pheretima sp. dan Perionyx sp). Kepadatan relatif cacing tanah di lahan pertanian yaitu Pontoscolex sp. (66, 49 %) dan Amynthas sp. (33,51 %) dan pekarangan yaitu Pontoscolex sp. (68, 10%), Amynthas sp. (29,31 %), Pheretima sp. (1,72 %) dan Perionyx sp. (0,86 %). Kepadatan relatif cacing tanah yang lebih rendah di lahan pertanian dibandingkan lahan pekarangan dapat disebabkan oleh residu pestisida mankozeb (0,035 mg/kg) dan propineb (0,014 mg/kg). Faktor lain yang turut mempengaruhi yaitu serasah yang lebih sedikit di lahan pertanian tomat dibandingkan di lahan pertanian. Kata kunci: cacing tanah, kepadatan relatif, pestisida, tomat, Sulawesi Utara   Abstract The application of pesticides is intended to maintain the production of tomato however this may affect the density of earthworms. The aim of research was to analyze the density of earthworms in tomato farming applied pesticide at Ampreng Village, Langowan West Sub-District - North Sulawesi. The results showed that earthworms in the tomato farming were two taxa i.e Pontoscolex sp.and Amynthas sp and in the front yards were four taxa i.e. Pontoscolex sp Amynthas sp., Pheretima sp. and Perionyx sp). The relative density of earthworms in tomato farming were Pontoscolex sp (66, 49%) and Amynthas sp (33.51%) and front yards were Pontoscolex sp (68, 10%), Amynthas sp (29.31%), Pheretima sp (1, 72%) and Perionyx sp (0.86%). The relative density of earthworms in tomato farming was lower than front yards caused by residue of mancozeb (0.035 mg/kg) and propineb (0,014 mg/kg) and the litter factor. Tomato farming have smaller litter than front yard that reduced the density of earthworm. Keywords: earthworm, relative density, pesticide, tomatoes, North Sulawesi
Induksi Mutasi Kromosom dengan Kolkisin Pada Bawang Putih (Allium sativum L.) Kultivar ‘Kesuna Bali’ (Induced Chromosome Mutation Using Colchicine in Garlic (Allium sativum Linn.) Cultivar ‘Kesuna Bali’) Pharmawati, Made; Wistiani, Ni Luh Ayu Jami
JURNAL BIOS LOGOS Vol 5, No 1 (2015): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.5.1.2015.9317

Abstract

Abstrak Tanaman bawang putih (Allium sativum L.) adalah tanaman holtikultura yang memiliki banyak manfaat terutama umbinya yang umumnya digunakan sebgai bumbu dan obat. Salah satu kultivar bawang putih yang ditanam di Bali adalah ‘Kesuna Bali’ yang hanya memiliki satu siung.  Salah satu cara untuk memperbaiki karakter tanaman adalah dengan cara induksi mutasi kromosom dengan kolkisin. Penelitian ini bertujuan menganalisis pengaruh perlakuan kolkisin terhadap indeks stomata dan jumlah kromosom dari tanaman ‘Kesuna Bali’. Penelitian menggunakan Rancangan Acak Kelompok dengan enam ulangan. Perlakuan kolkisin yang digunakan adalah 5%, 10% dan 20%. Hasil penelitian menunjukkan bahwa perlakuan kolkisin menurunkan indeks stomata dan meningkatkan jumlah kromosom. Kromosom triploid (2n=3x=24) dihasilkan pada perlakuan kolkisin 20%. Kata kunci : Allium sativum L., ’Kesuna Bali”, kolkisin, mutasi, sitologi Abstract Garlic (Allium sativum L.) is a horticultural crop that has many benefits, especially as spice and traditional medicine.  One of garlic cultivars planted in Bali is ‘Kesuna Bali’ which only has one clove. To improve characters of ‘Kesuna Bali’, modification of ‘Kesuna Bali’ properties can be done by means of induced mutation using colchicine.  This research aims to analyse the effect of colchicine on stomata index and the number of chromosomes of 'Kesuna Bali'. This research used randomized block design with six replicates. In this experiment the concentration of colchicine used were 5%, 10% and 20%. The results of this study showed that colchicine treatment of 20% resulted in the lowest stomata index and there was an increase in chromosome number. Colchicine at concentration of 20% resulted in triploid chromosome set (2n = 3x = 24). Keywords: Allium sativum L., ’Kesuna Bali”, cholchicine, mutation, cytology
Penentuan Ukuran Kelompok dan Densitas Tangkasi (Tarsius tarsier) di Cagar Alam Tangkoko Batuangus, Kota Bitung, Sulawesi Utara (Group Size and Density Determination of Tankasi (Tarsius tarsier) in The Nature Reserve Tangkoko Batuangus, Bitung, North Su Lumente, Ahmadin Awal; Saroyo, Saroyo; Wahyudi, Lalu; Papu, Adelfia
JURNAL BIOS LOGOS Vol 5, No 1 (2015): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.5.1.2015.8237

Abstract

Abstrak Tangkasi (Tarsius tarsier) merupakan primata primitif (Prosimi) endemik di Pulau Sulawesi dan beberapa pulau satelitnya. Mereka menempati beberapa tipe habitat, antara lain hutan primer dan sekunder, semak, mangrove, dan pertanian. Organisasi sosialnya ialah keluarga monogami hingga multimale-multifemale. Penelitian ini dilaksanakan dengan tujuan untuk menentukan ukuran kelompok tangkasi. Penelitian dilaksanakan di Cagar Alam Tangkoko Batuangus pada bulan Juli sampai Agustus 2012. Metode yang digunakan ialah survei pada sarang tangkasi dan dilakukan penghitungan secara sensus. Luas area pengamatan ialah 100 ha yang dibagi menjadi 100 plot berukuran 100 m x 100 m. Waktu pengamatan ialah pada pagi hari pada jam 05.00-06.00 untuk menentukan lokasi sarang pada satu plot, yang selanjutnya dilakukan sensus pada sore harinya. Hasil penelitian menunjukkan bahwa di dalam 100 ha ditemukan 41 sarang atau tempat tidur tangkasi, rata-rata ukuran kelompok ialah 4,95 ± 2,26 ekor dengan modus 4. Kata kunci: ukuran kelompok, tangkasi (Tarsius tarsier), Cagar Alam Tangkoko Batuangus Abstract Tangkasi (Tarsius tarsier) is a primitive species of Sulawesi primates that is distributed in Sulawesi Island and some satellite islands. This species occupies in several habitat types, such as primary and secondary forest, shrub, mangrove, and farming area. Their social organization is monogamous family to multi-male/multi-female group. This study was conducted to determine the group size of tangkasi in the Tangkoko Batuangus Nature Reserve from July to August 2012. The survey method was used in the tangkasi nests and the census measurement was applied to the total of individuals at each nest of the 100 ha area. The 100-ha-survey area was divided into 100 plots and the size of each plot was 100 m x 100 m. The observation was carried out to determine the nest location in each plot every morning (05.00-06.00 am) and followed by the census in the afternoon. The results showed that there were 41 groups of tangkasi in the 100 ha area, the average of group size was 4,95 ± 2,26 and the modus was 4. Keywords: group size, tangkasi (Tarsius tarsier), Tangkoko Batungus Nature Reserve
Optimasi Konsentrasi DNA dan MgCl2 pada Reaksi Polymerase Chain Reaction-Random Amplified Polymorphic DNA untuk Analisis Keragaman Genetik Tanaman Faloak (Sterculia quadrifida R.Br) (Optimization of DNA and MgCl2 Concentrations in Polymerase Chain Reacti Uslan, Uslan; Pharmawati, Made
JURNAL BIOS LOGOS Vol 5, No 1 (2015): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.5.1.2015.9316

Abstract

Abstrak Faloak  merupakan tanaman yang tumbuh di lahan kritis. Sebagai upaya mendukung pemuliaan dan konservasi tanaman faloak diperlukan informasi keragaman genetiknya. Salah satu metode analisis keragaman genetik adalah menggunakan penanda DNA yang berbasis PCR. Untuk itu diperlukan kondisi PCR (Polymerase Chain Reaction) yang tepat sehingga diperoleh hasil yang dapat dianalisis lebih lanjut. Penelitian ini bertujuan menentukan kondisi optimum PCR-RAPD (Polymerase Chain Reaction-Random Amplified Polymorphic DNA) tanaman faloak. Ekstraksi DNA dilakukan dengan metode CTAB. Optimasi dilakukan dengan menggunakan beberapa konsentrasi DNA cetakan dan MgCl2. Kondisi optimum PCR-RAPD tanaman faloak yang menghasilkan pita produk PCR yang jelas diperoleh  menggunakan 50 ng/ul DNA, 3 mM MgCl2 serta jumlah siklus termal 45 x. Kata kunci : PCR-RAPD, optimasi, tanaman faloak Abstract Faloak is a plant that grows on critical lands. In an effort to support breeding and conservation of faloak, information about its genetic diversity is required. One of the methods of genetic diversity analysis is using PCR-based DNA markers. For that purpose, proper PCR conditions is needed in order to obtain results that can be further analyzed. This study aimed to determine the optimum conditions for PCR-RAPD of faloak plants. DNA extraction was conducted using CTAB. Optimization was done by using several concentrations of DNA templates and MgCl2. The optimum conditions of PCR-RAPD of faloak plants that produce clear band of PCR products were obtained using 50 ng/ ul DNA, 3 mM MgCl2 and 45x thermal cycles Keywords : PCR-RAPD, optimization, faloak plant

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