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INDONESIA
Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : 24422606     EISSN : 2548611X     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
JBBI, Indonesian Journal of Biotechnology & Bioscience, is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, as well as life sciences in general. Initiated at the then Biotech Centre, the journal is published by the Laboratory for Biotechnology, the Agency for the Assessment and Application of Technology, BPPT.
Arjuna Subject : -
Articles 12 Documents
 1   2 
Search results for , issue " Vol 2, No 2 (2015): December 2015" : 12 Documents clear
PRODUKSI BIOETANOL DARI BATANG TUA KELAPA SAWIT DENGAN AIR PANAS BERTEKANAN DAN BALL MILL ., Suyanto; Matsunaga, Kotetsu; Inoue, Hiroyuki; Sakanishi, Kinya
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 2, No 2 (2015): December 2015
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (618.712 KB) | DOI: 10.29122/jbbi.v2i2.508

Abstract

Bioethanol from oil palm trunk is one important source of energy to reduce greenhouse gas emissions and to avoid competition between the use for food and for energy as happens in other commodities. Pre-treatment of lignocellulosic biomass using sulfuric acid has high efficiency but requires high cost to neutralize the byproducts formed. Pre-treatment using hot compressed water and ball mill can be used to break down the structure and composition of lignocellulose so as to increase the rate of enzymatic hydrolysis and increase the sugar yield. Hydrolysis using an enzyme cocktail with hot compressed water and ball mill can each produce 210 mg of glucose at a temperature of 220°C and 200 mg of glucose for 120 minutes at room temperature per gram of oil palm trunk. Both of these processes are environmentally friendly and have a high efficiency in the production of bioethanol from oil palm trunks.Keywords: bioethanol, oil palm trunk, ball mill, hot compressed water, hydrolysis  ABSTRAKBioetanol dari batang kelapa sawit adalah salah satu sumber energi yang penting untuk mengurangi emisi rumah kaca dan menghindari konflik kepentingan antara kebutuhan bahan pangan dan penggunaan energi sebagaimana terjadi pada komoditas lain. Pengolahan awal biomassa lignoselulosa menggunakan asam sulfat memiliki efisiensi yang tinggi tetapi membutuhkan biaya yang tinggi guna menetralisisr produk samping yang terbentuk. Pengolahan awal menggunakan air panas bertekanan dan ball mill dapat digunakan untuk memecah struktur dan komposisi lignoselulosa sehingga dapat meningkatkan laju hidrolisis enzim dan meningkatkan hasil gula. Hidrolisis menggunakan multienzim dengan air panas bertekanan dan ball mill masing-masing dapat menghasilkan 210 mg glukosa pada suhu 220°C dan 200 mg glukosa selama 120 menit pada suhu kamar per gram batang kelapa sawit. Kedua proses tersebut ramah lingkungan dan memiliki efisiensi yang tinggi pada produksi bioetanol dari batang kelapa sawit.Kata Kunci: bioetanol, batang kelapa sawit, ball mill, air panas bertekanan, hidrolisis 
KAJIAN ELONGASI PADA TANAMAN IN VITRO GAHARU (Aquilaria beccariana van Tiegh) Fauzan, Yusuf Sigit Ahmad; Sandra, Edhi; Mulyono, Daru
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 2, No 2 (2015): December 2015
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (737.168 KB) | DOI: 10.29122/jbbi.v2i2.511

Abstract

The population density of natural agarwood (Aquilaria beccariana) in Indonesia decreased to less than one tree per hectare. Efforts have been carried out on ex situ conservation of agarwood despite facing many obstacles. In vitro propagation is one alternative to speed up the recovery of natural agarwood populations. The purpose of this study was to obtain optimal elongation media for in vitro culture with addition of auxin and cytokinin, namely IBA, BAP and kinetin. The results showed that the best auxin-cytokinin combination was IBA 0.1 mg/L and BAP 0.05 mg/L. This combination increased the height and number of segments of A. beccariana with an average height of 1.64 cm and average number of sections of 6.40. It is suggested that this combination of IBA and BAP was the most effective compared to the other treatments. In addition, the combination of IBA 0 mg/L and BAP 0.03 mg/L gave rise to the best response to increase the number of shoots with an average of 1.91 shoots.Keywords: Aquilaria beccariana, shoot, elongation, auxin, cytokinin ABSTRAKKepadatan populasi gaharu (Aquilaria beccariana) alam di Indonesia kurang dari satu pohon per hektar. Upaya pelestarian gaharu ex situ telah banyak dilakukan tetapi masih banyak kendala. Perbanyakan gaharu in vitro merupakan salah satu cara alternatif untuk mempercepat pemulihan populasi gaharu alam. Tujuan penelitian ini adalah untuk memperoleh media elongasi yang optimal pada kultur in vitro gaharu dengan penambahan kombinasi zat pengatur tumbuh auksin dan sitokinin. Pada penelitian ini digunakan auksin IBA, serta sitokinin BAP dan Kinetin. Hasil penelitian elongasi diperoleh kombinasi auksin dan sitokinin terbaik yaitu, IBA 0,1 mg/L dan BAP 0,05 mg/L. Kombinasi ini meningkatkan tinggi dan jumlah ruas Aquilaria beccariana dengan tinggi rata-rata sebesar 1,64 cm dan jumlah ruas rata-rata sebesar 6,40 ruas. Pada kombinasi dan taraf ini diduga mekanisme kerja IBA dan BAP paling efektif dibanding perlakuan yang lain. Sedangkan kombinasi IBA 0 mg/L dan BAP 0,03 mg/L memberikan respon terbaik terhadap peningkatan jumlah tunas dengan rata-rata sebanyak 1,91 tunas.Kata Kunci: Aquilaria beccariana, tunas, elongasi, auksin, sitokinin

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