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Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : 24422606     EISSN : 2548611X     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
JBBI, Indonesian Journal of Biotechnology & Bioscience, is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, as well as life sciences in general. Initiated at the then Biotech Centre, the journal is published by the Laboratory for Biotechnology, the Agency for the Assessment and Application of Technology, BPPT.
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Articles 18 Documents
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Search results for , issue "Vol. 7 No. 2 (2020): December 2020" : 18 Documents clear
PENGARUH METODE PROSES SINTESIS ANALOG KURKUMIN ASIMETRIS TERHADAP EFEK HEPATOPROTEKTIF MENCIT (Mus musculus L.) Imanuel Berly Delvis Kapelle; Wasmen Manalu
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 2 (2020): December 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1337.743 KB) | DOI: 10.29122/jbbi.v7i2.4206

Abstract

Effect of Synthesis Process Method of Asymmetric Curcumin Analog on the Hepatoprotective Effect of Mice (Mus musculus L.) Asymmetric curcumin analogs (ACA) can be synthesized from cullilawan oil. ACA products can be synthesized using conventional methods and microwaves. Synthesis methods can affect physical properties and pharmacological effects. The purpose of this study was to determine the effect of the process method on the hepatoprotective ability of ACA and to determine the effective dose. ACA products were tested in vivo in mice (Mus musculus L.) with CCl4-induced liver damage. The parameters observed were biochemical liver enzymes (AST and ALT) and histological analysis. The results showed that animals treated with ACA-k products had better weight gain, lower AST and ALT levels, and fewer histological signs of liver damage at higher ACA doses than those observed in animals that were untreated or treated with ACA-m products. Thus, asymmetric curcumin analog products that were processed by conventional method were more hepatoprotective. Keywords: ACA, culilawan oil, hepatoprotection, in vivo, product diversification ABSTRAK Analog kurkumin asimetris (AKAS) dapat disintesis dari minyak kulit lawang. Produk AKAS dapat disintesis menggunakan metode konvensional dan microwave. Metode sintesis dapat mempengaruhi sifat fisik dan efek farmakologis. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh metode proses pada kemampuan hepatoprotektif AKAS dan untuk menentukan dosis efektif. Produk AKAS diuji in vivo pada mencit (Mus musculus L.) dengan kerusakan hati yang diinduksi CCl­4. Parameter yang diamati adalah biokimia enzim hati (AST dan ALT) dan analisis histologis. Hasil penelitian menunjukkan bahwa hewan yang diobati dengan produk AKAS-k memiliki kenaikan berat badan yang lebih baik, tingkat AST dan ALT yang lebih rendah, dan lebih sedikit tanda histologis kerusakan hati pada dosis AKAS yang lebih tinggi daripada yang terlihat pada hewan yang tidak diperlakukan atau diobati dengan produk AKAS-m. Dengan demikian, produk analog kurkumin asimetris yang diproses dengan metode konvensional lebih hepatoprotektif.
TELAAH KOMPREHENSIF DIABETES MELITUS: KLASIFIKASI, GEJALA, DIAGNOSIS, PENCEGAHAN, DAN PENGOBATAN: A Comprehensive Review of Diabetes Mellitus: Classification, Symptoms, Diagnosis, Prevention, and Treatment Dudi Hardianto
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 2 (2020): December 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340.815 KB) | DOI: 10.29122/jbbi.v7i2.4209

Abstract

Diabetes mellitus is a metabolic disorder characterized by hyperglycemia. In general, diabetes is classified into type 1 diabetes mellitus (T1DM), type 2 diabetes mellitus (T2DM), gestational, and other specific diabetes. The causes of diabetes are genetic disorders and environmental. Common symptoms of diabetes include: polydipsia, polyphagia, glycosuria, polyuria, dehydration, fatigue, weight loss, reduced vision, cramps, constipation, and candida infection. Test for diagnosis of diabetes include: fasting plasma glucose test, plasma glucose test after 2 hours of 75 g oral glucose administration, the glycated hemoglobin test (HbA1C), and random blood glucose test. Prevention of T1DM is still difficult because of the limited knowledge of metabolic, genetic, and immunological processes in the development of T1DM. T2DM is prevented by lifestyle and medical intervention. Insulin is the only drug for T1DM, whereas T2DM is treated with metformin as drug’s primary choice for reducing blood glucose levels. Diabetes melitus merupakan penyakit kelainan metabolisme yang ditandai dengan hiperglikemia. Secara umum, diabetes diklasifikasikan menjadi: diabetes melitus tipe 1 (DMT1), diabetes melitus tipe 2 (DMT2), gestasional, dan diabetes spesifik lain. Penyebab diabetes adalah kelainan genetik dan lingkungan. Gejala umum diabetes antara lain: polidipsia, polifagia, glikosuria, poliuria, dehidrasi, kelelahan, penurunan berat badan, daya penglihatan berkurang, kram, konstipasi, dan infeksi candida. Pemeriksaaan untuk diagnosis diabetes meliputi: pemeriksaan glukosa plasma saat puasa, pemeriksaan glukosa plasma setelah 2 jam pemberian glukosa oral 75 g, pemeriksaan hemoglobin terglikasi (HbA1C), dan pemeriksaan glukosa darah acak. Pencegahan DMT1 masih sulit karena terbatasnya pengetahuan proses metabolisme, genetik, dan imunologi pada perkembangan DMT1. DMT2 dicegah dengan intervensi gaya hidup dan intervensi medis. Insulin merupakan satu-satunya obat untuk DMT1, sedangkan DMT2 diobati dengan metformin sebagai pilihan utama dan non obat untuk menurunkan kadar glukosa dalam darah.
CONFIRMATION OF ENDOPHYTIC MICROBES CAUSING CONTAMINATION IN WATER SPINACH (Ipomoea aquatica Forssk.) TISSUE CULTURE Resa Sri Rahayu; Indriati Ramadhani; Masrukhin Masrukhin; Indira Riastiwi; Apriliana Dyah Prawestri; Yeni Yuliani
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 2 (2020): December 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1416.379 KB) | DOI: 10.29122/jbbi.v7i2.4381

Abstract

Konfirmasi Mikroba Endofit Penyebab Kontaminasi pada Kultur Jaringan Kangkung (Ipomoea aquatica Forssk.) Tanaman kangkung secara alami bersimbiosis dengan mikroba endofit, yang berpotensi menjadi kontaminan pada kultur jaringan kangkung karena berada di dalam jaringan dan sulit dijangkau saat proses sterilisasi eksplan. Tujuan penelitian ini adalah mengonfirmasi mikroba endofit penyebab kontaminasi pada kultur jaringan kangkung ‘Tetraploid’ sehingga dapat menjadi informasi awal untuk metode sterilisasi yang efektif. Sebanyak 14 sampel kontaminan pada media tanam kultur jaringan kangkung diisolasi dan diidentifikasi secara molekuler berdasarkan gen 16S rDNA untuk bakteri, daerah D1/D2 dari gen LSU rRNA untuk khamir, dan daerah ITS dari gen rDNA untuk jamur. Keragaman jenis mikroba yang teridentifikasi dibandingkan dengan keragaman jenis mikroba endofit dari jaringan tanaman kangkung yang ditanam pada media kultur jaringan tidak terkontaminasi mikroba, media kultur jaringan terkontaminasi mikroba, media tanam campuran tanah steril dan tidak steril, serta kangkung yang didapatkan dari pasar. Hasil penelitian ini mengonfirmasi bahwa khamir endofit dari kelompok Ustilaginaceae (basidiomycetous yeast) yang berasal dari jaringan tanaman kangkung sama dengan jenis kontaminan yang mengontaminasi media kultur jaringan kangkung ‘Tetraploid’. Khamir dari kelompok Ustilaginaceae (basidiomycetous yeast) tersebut juga merupakan mikroba paling dominan yang mengontaminasi media tanam kultur jaringan kangkung ‘Tetraploid’. Keywords: Ipomoea aquatica, kultur jaringan, mikroba endofit, morfologi, pohon filogeni Water spinach in nature lives in symbiosis with endophytic microbes, which have the potential to become contaminants in water spinach tissue culture because they are difficult to eliminate during the explant sterilization process. This study aimed to confirm endophytic microbes that cause contamination in the tissue culture of 'Tetraploid' water spinach so that it can provide initial information for an effective sterilization method. Fourteen contaminant samples in water spinach tissue culture media were isolated and identified molecularly based on the 16S rDNA gene for bacteria, the D1/D2 region of the LSU rRNA gene sequences for yeast, and ITS region of the rDNA gene for mold. The diversity of microbial species identified was compared with the diversity of endophytic microbial types from water spinach plant tissue grown on sterile tissue culture media, microbially contaminated tissue culture media, sterile and non-sterile soil mixed planting media, and water spinach obtained from the market. The results confirmed that endophytic yeast from Ustilaginaceae group (basidiomycetous yeast) derived from water spinach plant tissue was the same type of microbe that contaminated the 'Tetraploid' water spinach tissue culture media. The results gave new information that yeast from the Ustilaginaceae (basidiomycetous yeast) group was the most dominant microbe contaminating water spinach ‘Tetraploid’ tissue culture media. This group is endophytic yeast that lives within Ipomoea aquatica tissues.
DAYA REGENERASI KALUS DAN TUNAS IN VITRO PADI VARIETAS TARABAS PADA BERBAGAI KONSENTRASI 2,4-D Wahyu Indra Duwi Fanata; Dalliyah Hadrotul Qudsiyah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 2 (2020): December 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (953.92 KB) | DOI: 10.29122/jbbi.v7i2.4404

Abstract

In Vitro Callus and Plant Regeneration Rate of Tarabas Rice on Several Concentrations of 2,4-D The Agricultural Research and Development Agency and the West Java Provincial Government are developing new superior varieties with Japonica rice standards, namely the Tarabas variety. However, the equivalence of somatic embryogenesis ability of Tarabas rice with original Japonica variety has not been reported. In this study, the frequency of callus regeneration of Tarabas vs Hwayoung rice varieties was compared. Induction of callus from mature embryos with several concentrations of 2,4-D showed the same extent of callus formation in both rice varieties. Callus induced by 1 ppm of 2,4-D showed the higher rate of shoot formation. On the other hand, percentage of callus formation of Tarabas rice was not affected by the increase of 2,4-D concentrations and was able to show 100% regeneration rate  at the fourth week in the regeneration medium, although the shoot growth was not as fast as those from medium with 1 ppm 2,4-D. Therefore, these results suggest that Tarabas variety has a somatic embryogenesis capacity equivalent to that of japonica rice and has the potential as research objects in the field of biotechnology. Badan Penelitian dan Pengembangan Pertanian serta Pemerintah Provinsi Jawa Barat mengembangkan varietas unggul baru dengan standar padi Japonica yaitu varietas Tarabas. Namun, kesetaraan varietas Tarabas dengan varietas Japonica asli dalam kemampuan embriogenesis somatik belum dilaporkan. Penelitian ini membandingkan respons kultur jaringan antara beras Tarabas dan padi Japonica varietas Hwayoung. Induksi kalus dari embrio matang dengan beberapa konsentrasi 2,4-D menunjukkan respons pembentukan kalus yang sama pada kedua varietas padi. Kalus yang diinduksi 1 ppm 2,4-D menunjukkan laju pembentukan tunas yang lebih tinggi. Di sisi lain, kalus Hwayoung yang diinduksi konsentrasi 2,4-D yang lebih tinggi menunjukkan penghambatan dalam pembentukan tunas. Di lain pihak, pembentukan kalus padi Tarabas tidak terpengaruh oleh peningkatan konsentrasi 2,4-D dan mampu menunjukkan 100% laju regenerasi tanaman pada minggu keempat di media regenerasi walaupun pertumbuhan tunas tidak secepat pada perlakuan 1 ppm 2,4-D. Karena itu, hasil penelitian ini menunjukkan bahwa varietas Tarabas memiliki kapasitas embriogenesis somatik yang setara dengan padi japonica dan padi Tarabas mempunyai potensi sebagai obyek penelitian di bidang bioteknologi.
Back Cover JBBI Vol 7, No 2, December 2020 .
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 2 (2020): December 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

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Abstract

Front Cover JBBI Vol 7, No 2, December 2020 .
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 2 (2020): December 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

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Appendix JBBI Vol 7, No 2, December 2020: Keyword Index and Author Index .
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 2 (2020): December 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

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Preface JBBI Vol 7, No 2, December 2020: Foreword and Acknowledgement .
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 2 (2020): December 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

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