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Utilization of Katuk (Sauropus androgynus L Merr) meal and extract as ration substitution to produce low chollesterol Japanese Quail product Subekti, Sri; Piliang, Wiranda G; Manalu, Wasmen; Murdiati, Tri Budhi
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 4 (2006)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (131.636 KB) | DOI: 10.14334/jitv.v11i4.534

Abstract

Katuk (Sauropus androgynus L Merr) is known to contain carotenoids, vitamin E, vitamin C, protein and phytosterol compound. This study was aimed to determine the ability of phytosterols in Sauropus androgynus (SA) leaf to obtain poultry product that has low cholesterol content. One hundred fifty female quails were raised from 2-27 weeks old, divided into three treatment diets, five replicates with 10 quails in each replicate. The treatment diets were: 1) Control group: diet without katuk leaf meal; 2) Diet with 9% SA ethanol 70% extract (SAE); 3) Diet containing 9% SA meal (SAM). Kolesterols were determined with CHOD-PAP-Method by Human, there is enzymatic Colorimetric Test for Cholesterol with Lipid Clearing Factor. The result showed that total cholesterol in the egg yolk, carcass and liver of SAE and SAM treated quails were lower (P<0.05) than that of the control-treated quails, except the cholesterol content in the serum. These findings indicated that the cholesterol concentration of female quails was decreased due to phytosterol content in katuk leaf. Key Words: Phytosterol, Katuk Leaf, Kolesterol, Quails
Responses of milk quality to roasted soybeans, calcium soap and organic mineral supplementation in dairy cattle diets ., Adawiah; Sutardi, Toha; Toharmat, Toto; Manalu, Wasmen; ., Nahrowi
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 4 (2006)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (125.959 KB) | DOI: 10.14334/jitv.v11i4.538

Abstract

Milk quality is affected by feed nutrient either macronutrient or micronutrient.  Roasted soayabeans and calcium soap were to increase supply by pas protein and fat to dairy cattle. Thus, organic mineral was to increase bioavailability of feed mineral to animal.  The objective of this study was to evaluate roasted soybean, mineral soap and organic mineral supplementation on milk quality of dairy cattle.  Twenty lactating Frisian Holstein cows (initial weight 361.4 ± 40.39 kg) were assigned into a randomized complete block design with 5 treatments and 4 blocks. The treatments were A: basal diet, B: A + roasted soybean, C: B + calcium soap of corn oil, D: C + calcium soap of corn oil, E: C + calcium soap of fish oil. The experimental diets were offered for 9 and 2 weeks preliminary. The results of the experiment showed that milk protein and lactose were not affected by diets.  Milk dry matter  of cows fed A, B, and D diets were higher (P<0.05) than those of fed C and E diets.  Milk fat of cows fed A,  B and D diets were higher (P<0.05) than those of  fed C and E diets. Milk density of cows fed B and E diets were higher (p<0.05) than those of fed A, C and D diets. Milk TPC of cows fed B diet were higher (0.05) than those of fed A, C, D, and E diets. It is concluded that milk quality especially milk protein and lactose concentration are not affected by roasted soyabeans, Ca-soap, and organic mineral. Calcium soap of fish oil and organic mineral decrease population of milk bacteria. Key Words: Calcium Soap, Organic Mineral, Roasted Soyabeans, Dairy Cattle, Quality Milk
ETHOGRAM PERILAKU ALAMI INDIVIDU TIKUS SAWAH (Rattus argentiventer Robinson and Kloss, 1916) DALAM LABORATORIUM Anggara, Agus Wahyana; Solihin, Dedy Duryadi; Manalu, Wasmen; Irzaman, Irzaman
ZOO INDONESIA Vol 24, No 2 (2015): Desember 2015
Publisher : Masyarakat Zoologi Indonesia

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Abstract

Perilaku merupakan respons senso-motorik makhluk hidup terhadap beragam stimulus dan fluktuasi kondisi lingkungan. Pengamatan terhadap perilaku alami tikus sawah yang ditangkap dari lapangan dilakukan pada kondisi di dalam laboratorium. Percobaaan bertujuan untuk memetakan dan mendeskripsikan perilaku alami tikus sawah. Hasil percobaan diharapkan dapat menjadi dasar untuk menyusun metode manipulasi perilaku tikus sawah dalam rangka menyusun metode pengendaliannya. Semua aktivitas tikus percobaan dipantau kamera CCTV dan dilakukan pengamatan saksama untuk membuat ethogram. Hasil pengamatan menunjukkan bahwa aktivitas normal tikus sawah sepanjang periode aktifnya pada malam hari meliputi perilaku keluar-masuk lubang sarang, mengendus, mengawasi, menjelajah, makan dan minum, merawat diri, istirahat, dan menggali tanah. Sebagian besar aktivitas dilakukan pada pukul 17:30-22:00 WIB sehingga dapat dinyatakan bahwa puncak aktivitas tikus sawah berlangsung pada periode petang hari.Kata kunci:aktivitas harian, nokturnal, laboratorium, pengendalian
PERBANDINGAN KARAKTER MERISTIK PADA Varanus salvator macromaculatus Deraniyagala, 1944 DARI POPULASI WILAYAH SUMATERA Setyawatiningsih, Sri Catur; Arida, Evy Ayu; Solihin, Dedy Duryadi; Boediono, Arief; Manalu, Wasmen
ZOO INDONESIA Vol 24, No 2 (2015): Desember 2015
Publisher : Masyarakat Zoologi Indonesia

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Abstract

Karakter meristik (hitung sisik) merupakan karakter pendiagnosa dalam mencandra biawak, termasuk Varanus salvator complex. V. s. macromaculatus tersebar paling luas di antara anak jenis Biawak Air sehingga diduga memiliki variasi morfologi. Hal tersebut ditunjukkan adanya ketumpangtindihan hitung sisik V.s. macromaculatus dengan anak jenis lainnya. Maka hitung sisik bukan sebagai karakter pendiagnosa yang mandiri. Oleh karenya digunakan pola warna sebagai karakter pendiagnosa lainnya. Penelitian ini bertujuan untuk membandingkan karakter hitung sisik dan pola warna tubuh V.s. macromaculatus asal Pulau Sumatera dan pulau-pulau satelitnya serta Pulau Jawa untuk melihat kekonsistenan dan kemandirian karakter meristik pada anak jenis tersebut. Kami juga mengidentifikasi karakter hitung sisik yang membedakan dua anak jenis, yaitu V.s. macromaculatus dan V.s. bivittatus. Karakter hitung sisik di bagian tengah tubuh (karakter S) dan pola warna tubuh dapat digunakan untuk membedakan populasi Biawak Air asal Pulau Simeulue dengan setiap populasi yang diteliti (populasi asal Pulau Sumatera, Pulau Batam, Pulau Kundur, dan Pulau Jawa). Karakter S bersifat konsisten dan bukan sebagai karakter pendiagnosa mandiri. Hitung sisik di sekitar pangkal ekor (karakter Q) dapat membedakan anak jenis Biawak Air, yaitu V.s. macromaculatus dan V.s. bivittatus dalam penelitian ini.
Production of Superior Pigs by Injecting the Sows with Gonadotropin Prior to Mating Rayer, Debby Jacqueline Jochebed; Maheswari, Hera; Muladno, Muladno; Manalu, Wasmen
ANIMAL PRODUCTION Vol 17, No 1 (2015): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.862 KB) | DOI: 10.20884/1.anprod.2015.17.1.475

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Abstract. An experiment was conducted to produce superior pigs with improved growth phenotypes and survival during postnatal growth by injecting the sows with gonadotropin prior to mating.  The experiment consisted of 2 stages.  In the first stage, 12 sows were divided into 2 groups i.e., sows injected with NaCl0.95% as a control (NSO) and sows injected with PG600 (SO) prior to mating.  Parameters measured were growth phenotypes and survival at birth and during pre-weaning period.  In the second stage, 24 of weaned pigs (age 8 weeks) from the first stage were selected (6 males and 6 females from NSO group and 6 males and 6 females from SO group) to be used for measurement of growth performance.  The experimental pigs were raised and observed until the age of 28 weeks (7 month).  The body weights were measured monthly.  The results of the experiment showed that improvement of uterine environment by gonadotropin injection of the sows prior to mating dramatically improved birth weight with a very homogenous birth weight within litter size.  Improved birth weight and within-litter variation of birth weight improved pre-weaning growth performance and survival that finally dramatically increased weaning weight and total weight of weaned pigs per sow.  After weaning, pigs born to SO sows grew faster and had around 10 kg higher body weight as compared to control at the age of 7 month.   It was concluded that superior pigs could be produced by improving endogenous secretion of pregnant hormone during pregnancy. Key words: Birth weight, growth phenotypes, survival, prenatal growth, postnatal growth Abstrak. Penelitian ini dilakukan untuk menghasilkan anak-anak babi unggul dengan fenotipe pertumbuhan dan daya hidup yang lebih baik selama pertumbuhan pascalahir dengan cara menyuntik induk babi dengan gonadotropin sebelum pengawinan.  Penelitian terdiri atas dua tahapan.  Penelitian tahap pertama, 12 ekor induk babi dikelompokkan ke dalam 2 kelompok, 1) kelompok kontrol, yaitu induk yang disuntik dengan NaCl 0.95% (NSO) dan 2) kelompok yang disuntik dengan PG600 sebelum pengawinan (SO).  Parameter yang diukur ialah fenotipe pertumbuhan dan daya hidup anak pada saat lahir dan selama periode prasapih.  Tahap kedua, 24 anak babi lepas sapih dari percobaan tahap pertama (umur 8 minggu) dipilih (6 jantan dan 6 betinadari kelompok NSOdan 6 jantan dan 6 betina dari kelompok SO) untuk digunakan dalam pengukuran kinerja pertumbuhan pascasapih.  Babi percobaan dibesarkan dan diamati sampai umur 28 minggu (7 bulan).  Bobot badan diukur setiap bulan.  Hasil penelitian menunjukkan bahwa perbaikan lingkungan uterus dengan cara penyuntikan induk dengan gonadotropin sebelum pengawinan secara dramatis memperbaiki bobot lahir anak dengan bobot lahir per induk yang lebih seragam.  Perbaikan bobot lahir dan keseragaman bobot lahir memperbaiki kinerja pertumbuhan prasapih dan kelangsungan hidup anak yang pada akhirnya secara dramatis meningkatkan bobot sapih dan total bobot anak yang disapih per ekor induk.  Setelah penyapihan, anak babi yang dilahirkan oleh induk yang disuntik gonadotropin tumbuh lebih cepat dengan bobot badan yang lebih tinggi sekitar 10 kg dibandingkan dengan kontrol pada umur 7 bulan.  Disimpulkan bahwa anak babi unggul dapat dihasilkan melalui perbaikan sekresi endogen hormon kebuntingan selama kebuntingan. Kata kunci: Bobot lahir, fenotipe pertumbuhan, daya hidup, pertumbuhan prenatal, pertumbuhan pascalahir
POTENSI EKSTRAK PEGAGAN (Centella Asiatica) DAN KUNYIT (Curcuma longa) UNTUK MENINGKATKAN AKTIVITAS ENZIM GLUTATION PEROKSIDASE (GSH Px) PADA JARINGAN HATI TIKUS [Potency of Centella asiatica and Curcuma longa Extracts in Increasing Glutathione Peroxidase (GSH-Px) Enzyme Activities in The Liver Tissue of Rats] Aswani, Tuti; Manalu, Wasmen; Suprayogi, Agik; Rahminiwati, Min
BERITA BIOLOGI Vol 14, No 3 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (714.572 KB) | DOI: 10.14203/beritabiologi.v14i3.1832

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 This experiment was designed to study the effect of pegagan (Centella asiatica) and turmeric (Curcuma longa) extracts on the activities of glutathione peroxidase (GSH- Px) in liver of white Spraque Dawley rat aged 2 months old with average weight of 200 grams. Pegagan was extracted using infuse method, and turmeric was extracted using dekokta method. The activity of GSH-Px was measured using Flohe and Gunzler’s methods. Extract pegagan and turmeric were assumed to have both preventive and curative treatments. For the preventive treat- ment, extract pegagan and turmeric at concentrations of 22.05 mg/ml : 184.1 mg/ml could increased the activity of GSH-PX enzyme, i.e. 232.60 ± 21.40 mU / mgprotein. This was approximately 79% than the levels of GSH- Px enzyme in normal liver without paracetamol which was 190.78 ± 9.28 mU/mgprotein. For the curative treatment, extract pegagan and tumeric at concentration of 22.05 mg/ml : 184.1 mg/ml could improved GSH- Px enzyme activity (i.e. 239.01 ± 47.40 mU/ mgprotein). This figure was about 92% higher than the levels of GSH-Px enzyme in the liver normal without paracetamol which was 190.78 ± 9.28 mU/mg protein. Pegagan and turmeric extracts with a high concentration could increased the activity of GSH-Px enzyme, thus potential as curative treatment.
PERUBAHAN SEBARAN SEL-SEL ASIDOFIL DAN BASOFIL HIPOFISA PENGARUH PEMBERIAN PASAK BUMI (Eurycoma longifolia Jack) Pratomo, Hurip; Supriatna, Iman; Manalu, Wasmen; Winarto, Adi
Jurnal Matematika Sains dan Teknologi Vol 12 No 2 (2011)
Publisher : LPPM Universitas Terbuka

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Abstract

Pasak bumi is popular as an aphrodisiac in the traditional medical herb. Boiled drink water of the root powder of pasak bumi is believed can increase the secondary reproduction characters such as libido and the duration of erection. This article is part of the research report that focus on: the distribution of hypophysis acidophil and basophil cells caused of the pasak bumi optimal dose treatment. Research carried out in an experiment using Hemaktosilin Eosin(HE) staining method to study micromorphological distribution of the acidophil and basophil cells of the male rat hypophysis after pasak bumi treatment (the dose of 18 mg pasak bumi/200 g body weight during three days, and the control of 1 ml aquadest). Result of the research showed that: Basophyl cells increased significantly in the third days of pasak bumi administered. Meanwhile acidophyl cells decreased in the third days of pasak bumi administered.
Profil Hematologis Tikus Putih (Rattus novergicus sp.) Betina Setelah Pemberian Jamu Kesuburan Rosdianto, Aziiz Mardanarian; Manalu, Wasmen; Maheshwari, Hera
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 8, No 1 (2016)
Publisher : Indonesian Research Gateway

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Abstract

ABSTRACT: The use of herbal preparations to improve fertility were empirically beneficial for reproductive health, however its use needs to be supported by scientific evidence. In this study we observed the effects of herbal preparations on haematological parameters, liver function and kidney function of female rats. The estrus cycle of the experimental female rats were previously synchronized by injection with prostaglandin twice in two days interval. The rats were randomly assigned into eight groups i.e., two negative control groups, two positive control groups (injected with pregnant mare serum gonadotropin with effective and double of effective doses), two groups were given herbal preparation A (with effective and double of effective doses), and two groups were given herbal preparation B (with effective and double of effective doses). The treatments were given for 3 consecutive estrous cycles. Parameters measured were hematological parameters (erythrocyte, leukocyte, and hemoglobin), liver functions (SGOT and SGPT concentrations), and renal function (serum ureum concentration). The results showed that the administration of herbal preparation for fertility improved erythrocyte, leukocyte, and hemoglobin parameters, improved liver functions, and optimized renal functions. It was concluded that the administration of herbal preparation for fertility for 3 consecutive estrous cycles did not disturb physiological functions, instead its improved hematological parameters as well as liver and renal functions.Keywords: hematological, herbal, liver function, renal function, female rats  ABSTRAK: Penggunaan jamu kesuburan secara empiris bermanfaat untuk kesehatan reproduksi, namun penggunaannya perlu didukung oleh data ilmiah. Dalam penelitian ini dilakukan pengamatan efek pemberian jamu kesuburan terhadap parameter hematologis, fungsi hati dan fungsi ginjal dari tikus betina. Penelitian ini diawali dengan sikronisasi siklus estrus tikus percobaan dengan penyuntikan prostaglandin dua kali dalam selang waktu dua hari. Tikus-tikus percobaan secara acak dibagi ke dalam delapan kelompok, yaitu dua kelompok kontrol negatif yang hanya diberi pelarut persiapan jamu kesuburan, dua kelompok kontrol positif yang disuntik pregnant mare serum gonadotropin untuk dosis efektif dan 2x dosis efektif, dua kelompok diberi jamu kesuburan A dengan dosis efektif dan 2x dosis efektif, dan dua kelompok yang diberi jamu kesuburan B untuk dosis efektif dan 2x dosis efektif. Perlakuan diberikan selama 3 siklus estrus berturut-turut. Parameter yang diukur adalah status hematologi (eritrosit, hemoglobin, dan leukosit), fungsi hati (kadar SGOT dan SGPT), dan fungsi ginjal (kadar ureum). Hasil penelitian menunjukkan bahwa pemberian jamu kesuburan memperbaiki parameter hematologis, memperbaiki fungsi hati, dan mengoptimalkan fungsi ginjal. Dari hasil penelitian disimpulkan bahwa pemberian jamu kesuburan selama tiga siklus estrus berturut-turut tidak mengganggu fungsi fisiologis tubuh bahkan terbukti memperbaiki parameter hematologis, fungsi hati, dan fungsi ginjal. Kata kunci: Jamu kesuburan, parameter hematologis, fungsi ginjal, fungsi hati, tikus betina 
Profil Hematologis Tikus Putih (Rattus novergicus sp.) Betina Setelah Pemberian Jamu Kesuburan Rosdianto, Aziiz Mardanarian; Manalu, Wasmen; Maheshwari, Hera
Jurnal Farmasi Indonesia Vol 8, No 1 (2016)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (413.351 KB) | DOI: 10.35617/jfi.v8i1.377

Abstract

ABSTRACT: The use of herbal preparations to improve fertility were empirically beneficial for reproductive health, however its use needs to be supported by scientific evidence. In this study we observed the effects of herbal preparations on haematological parameters, liver function and kidney function of female rats. The estrus cycle of the experimental female rats were previously synchronized by injection with prostaglandin twice in two days interval. The rats were randomly assigned into eight groups i.e., two negative control groups, two positive control groups (injected with pregnant mare serum gonadotropin with effective and double of effective doses), two groups were given herbal preparation A (with effective and double of effective doses), and two groups were given herbal preparation B (with effective and double of effective doses). The treatments were given for 3 consecutive estrous cycles. Parameters measured were hematological parameters (erythrocyte, leukocyte, and hemoglobin), liver functions (SGOT and SGPT concentrations), and renal function (serum ureum concentration). The results showed that the administration of herbal preparation for fertility improved erythrocyte, leukocyte, and hemoglobin parameters, improved liver functions, and optimized renal functions. It was concluded that the administration of herbal preparation for fertility for 3 consecutive estrous cycles did not disturb physiological functions, instead its improved hematological parameters as well as liver and renal functions.Keywords: hematological, herbal, liver function, renal function, female rats  ABSTRAK: Penggunaan jamu kesuburan secara empiris bermanfaat untuk kesehatan reproduksi, namun penggunaannya perlu didukung oleh data ilmiah. Dalam penelitian ini dilakukan pengamatan efek pemberian jamu kesuburan terhadap parameter hematologis, fungsi hati dan fungsi ginjal dari tikus betina. Penelitian ini diawali dengan sikronisasi siklus estrus tikus percobaan dengan penyuntikan prostaglandin dua kali dalam selang waktu dua hari. Tikus-tikus percobaan secara acak dibagi ke dalam delapan kelompok, yaitu dua kelompok kontrol negatif yang hanya diberi pelarut persiapan jamu kesuburan, dua kelompok kontrol positif yang disuntik pregnant mare serum gonadotropin untuk dosis efektif dan 2x dosis efektif, dua kelompok diberi jamu kesuburan A dengan dosis efektif dan 2x dosis efektif, dan dua kelompok yang diberi jamu kesuburan B untuk dosis efektif dan 2x dosis efektif. Perlakuan diberikan selama 3 siklus estrus berturut-turut. Parameter yang diukur adalah status hematologi (eritrosit, hemoglobin, dan leukosit), fungsi hati (kadar SGOT dan SGPT), dan fungsi ginjal (kadar ureum). Hasil penelitian menunjukkan bahwa pemberian jamu kesuburan memperbaiki parameter hematologis, memperbaiki fungsi hati, dan mengoptimalkan fungsi ginjal. Dari hasil penelitian disimpulkan bahwa pemberian jamu kesuburan selama tiga siklus estrus berturut-turut tidak mengganggu fungsi fisiologis tubuh bahkan terbukti memperbaiki parameter hematologis, fungsi hati, dan fungsi ginjal. Kata kunci: Jamu kesuburan, parameter hematologis, fungsi ginjal, fungsi hati, tikus betina 
Kajian Fisiologis Status Kalsium Puyuh (Coturnix Coturnix Japonica) setelah Pemberian Cahaya Monokromatik Kasiyati, Kasiyati; Kusumorini, Nastiti; Maheshwari, Hera; Manalu, Wasmen
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 18, No 1 (2010): Vol. XVIII, No. 1, Maret 2010
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (425.765 KB) | DOI: 10.14710/baf.v18i1.2588

Abstract

Deposited calsium makes of the eggshell is influnce of the light. The objective of the study was evaluate exposed of monochromatic light to obtain basic information of the physiology aspect of calcium satus in the quail which direct relation with quality of the eggshell. Two hundred and seventy quails were divided into nine treatments of light, with ten replications and three quails in each replication. The treatments were without light, controls with 15 and 25 W, red, green, and blue lights with intensities of 15 and 25 lux. Control treatment used incandescent bulb. The red, green, and blue lights were provided by light emitting diodes (LED). All lights treatment were given for 14 h daily, started from 17.00 to 07.00. Parameters measured were serum calcium concentrations, calcium contents in bone and the eggshell, weights and thichness of the eggshell. The data obtained were analyzed by analysis of variance (ANOVA), and the differences between treatments groups were subjected for Duncan’s Multiple Range Test (DMRT). All of the data analyzed were done GLM (general linear model) procedure in the SAS program. Quails exposed to monochromatic light had higher serum calcium concentrations and calcium contents in bone (P<0,05). Blue light could be used to increase quality of the eggshell.
Co-Authors - Hernawati . Safrida ., Chairul A Yani ABADI SUTISNA Abdul Zahid Adawiah . Ade Sunarma Ade Sunarma Adi Winanto Adi Winarto Adi Winarto Adi Winarto Agik Suprayogi Agus Oman Sudrajat Agus Wahyana Anggara Agus Wahyana Anggara Agus Wahyana Anggara, Agus Wahyana Ahmad Subhan Ahmad Yani ahmad yani Aisjah Girindra Alfred O. M. Dima Alifiana Fitrianingrum Amrozi Andi Mu’nisa Andri Yanto Andriyanto Andriyanto Andriyanto Andriyanto . Andriyanto A Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Anita Esfandiari Anuraga Jayanegara Arief Boediono Aryani Satyaningtijas Aswani, Tuti Aulia Andi Mustik Aulia Andi Mustika Aulia Andri Mustika Awaludin Azam B. Zaidy B P Purwanto B. P. Purwanto Bagus Priyo Purwanto Bambang Gunadi Bambang Gunadi Bambang Gunadi Bambang Kiranadi Bambang Kiranadi BAMBANG KIRANADI Bambang Pontjo Priosoeryanto Benny Heltonika Bondan Achmadi Cece Sumantri Chairul - Chairul Chairul Chairun Nisa Christina Clarice Leksono Cut Dara Dewi Damiana Rita Ekastuti Debby Jacqualine Jochebed Rayer Dedy D. Solihin Deni Noviana Deni Radona, Deni Desrial DEWI APRI ASTUTI Dewi Ratih Agung Priyono Dewi Ratih Agungpriyono Dewi Ratih Agungpriyono Dewi Ratih Agungpriyono Diah Nugrahani Pristihadi Diky Yuliansah Diky Yuliansah Dwi Gunadi Dwi Setyaningsih Dwi Utari Rahmiati Dyah Iswantini Edwin Ligia Sastra Ekowati Handharyani Elpita Tarigan Elpita Tarigan Elpita Tarigan Elpita Tarigan Elpita Tarigan Erli Chandra Etih Sudarnika Evy Ayu Arida EVY AYU ARIDA Fachriyan H. Pasaribu Firda Agustin Firda Agustin Firda Agustin, Firda Fitra Aji Pamungkas Fitra Aji Pamungkas Fitra Aji Pamungkas Fitria Senja Murtiningrum Friska Mery Montolalu Ganjar Maulana Nugraha HADI SUNARYO Hamdika Yendri Hamdika Yendri Hamdika Yendri Hamdika Yendri Hamdika Yendri Putra Hamdika Yendri Putra Hamdika Yendri Putra Hamdika Yendri Putra Hamdika Yendri Putra Hamny Sofyan Henni Syawal HERA MAHESHWARI Hera Maheswari, Hera Hernawati Hernawati Hesti Wahyuningsih Huda Shalahudin Darusman Hurip Pratomo Hurip Pratomo Husna, Hanifati I Ketut Mudite I Komang Gede Wiryawan Iman Hernaman Iman Supriatna Ira Agustina Dewi Gandasari Irfan Nurhidayat Irma Herawati Suparto Irzaman, Irzaman Isdoni Bustaman Jakaria Jakaria Jihan Fadilah Rachman Nurullah Jola J.M.R. Londok Joni Haryadi, Joni Kapelle, Imanuel B. D. Kardiyo Praptokardiyo Karin katina Hasya Kasiyati Kasiati Kharisma Mardathilah Kharisma Mardathilah Kiranadi Bambang koekoeh santoso Koekoeh Santoso Koekoeh Santoso Koeswinarning Sigit Komariah Komariah Komariah Krido Brahmo Putro Kusdiantoro Mohamad Kusumorini Nastiti La Eddy La Jumadin Leliana Nugrahaning Widi Leliana Nugrahaning Widi Leliana Nugrahaning Widi Leliana Nugrahaning Widi Leliana Nugrahaning Widi Leliana Nugrahaning Widi Leliana Widi Leliana Widi Lendrawati Lendrawati Leo Sapelani Soinbala Lies Parede Lina Noviyanti Sutardi Lis Rosmanah Lis Rosmanah Livana Dethris Rawung Lucia Johana Lambey Lynette Ong Huey M. Zairin Junior M.Y Sumaryadi Mas Yedi Sumaryadi Masriani . Meillisa Carlen Mainassy Mien Th. R. Lapian Mohamad Hasil Tamzil Mohamad Yamin Mohamad Yogie Hendrawan Mohammad Miftahurrohman Mokhamad Fakhrul Ulum, Mokhamad Muhaimin Hamzah MUHAMMAD AGUS SUPRAYUDI Muhammad Darjat Darulfalah Muhammad Junior Zairin Muhammad Zairin Jr Muhammad Zairin Jr. Muhammad Zairin Junior Murniaty Simorangkir Nabila Martha Ludi Miftahurahma Nahrowi Nahrowi Nastiti Kusomorini Nastiti Kusumorini Nastiti Kusumorini NASTITI KUSUMORINI NASTITI KUSUMORINI Nelda Fliza Zora Ni Made Ria Isriyanthi Nur Bambang Priyo Utomo Nur Bambang Priyo Utomo Nurhidayat - Nurhidayat, Nurhidayat Odang Carman Pollung Hasiholan Siagian PONIMAN PONIMAN Pudji Achmadi Purwantiningsih Sugita Putut I. Pudjiono R G Sianturi Raden Roro Sri Pudji Sinarni Dewi Rahminiwati, Min Rarah Ratih Adjie Maheswari Resi Milna Revolson Mege Riasari Gail Sianturi Riasari Gail Sianturi Riasari Gail Sianturi Ridi Arif RIDWAN AFFANDI Rindy Fazni Nengsih Rindy Fazni Nengsih Rindy Fazni Nengsih Rindy Fazni Nengsih Rindy Fazni Nengsih Risna Anggraeni Ronald Tarigan Ronald Tarigan Ronald Tarigan Ronny Rachman Noor Rosdianto, Aziiz Mardanarian Rosdianto, Aziiz Mardanarian Rudi Priyanto Rudi Priyanto Rudy Priyanto Rukmiasih Rukmiasih Ruqiah Ganda Putri Panjaitan Sabri Mustafa Safrida Safrida SATA YOSHIDA SRIE RAHAYU Sattabongkoch Maneewong Sela Septima Mariya Sharon Aurelia Sharon Aurelia Sharon Aurellia Silmy Kamila Widyanti Silmy Kamila Widyanti Sitti Wajizah Sri Catur Setyawatiningsih Sri Catur Setyawatiningsih Sri Nuryati Sri Subekti Subangkit, Mawar Suhendro, Ikhsan Sumiati Sumiati Sumiati Sumiati Sumiaty Aiba Sunarno . Sunarno s Sunarno Sunarno Sutiastuti Wahyuwardani Sutiastuti Wahyuwardani SYAHRUN HAMDANI NASUTION TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN TATI NURHAYATI Tiltje Andretha Ransaleleh TOHA SUTARDI Toto Toharmat Tri Budhi Murdiati Tri Budhi Murdiati Tuti Aswani Tutik Wresdiyati Umi Cahyaningsih Uus Saepuloh Wahyuni, Sri Wida Lesmanawati Widanarni Widanarni WIDANARNI WIDANARNI Wiranda G Piliang Wiranda G Piliang Wiranda G. Piliang Wiwin Winarsih Y. Hadiroseyani Yayuk Sri Rahayu Yetmaneli, Yetmaneli Yudha Trinoegraha Adiputra Yudha Trinoegraha Adiputra Yulia Yellita Yulvian Sani yuri, Nurasmi yuri Yusa Irarang Yusa Irarang Yusa Irarang Yusa Irarang Yusa Irarang Yusfiati Yusfiati Yuvensius Yuvensius Zulfa Zakiah