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Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : 24422606     EISSN : 2548611X     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
JBBI, Indonesian Journal of Biotechnology & Bioscience, is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, as well as life sciences in general. Initiated at the then Biotech Centre, the journal is published by the Laboratory for Biotechnology, the Agency for the Assessment and Application of Technology, BPPT.
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Articles 18 Documents
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Search results for , issue "Vol. 9 No. 1 (2022): June 2022" : 18 Documents clear
INHIBITORY ACTIVITY OF Trichoderma harzianum AGAINST PUTATIVELY PATHOGENIC FUNGUS ON RODENT TUBER (Typhonium flagelliforme) PLANT Rantika Silfarohana; Aji Wibowo; Nia Asiani; Zhafira Amila Haqqa; Mahmud Sugiyanto; Catur Sriherwanto
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022): June 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (562.029 KB) | DOI: 10.29122/jbbi.v9i1.5194

Abstract

Trichoderma spp. are globally considered as the most dominant biofungicide in the market. Reports on Trichoderma spp. efficacy against pathogenic fungi in commercial crops have been numerous, but much less in medicinal plants. This study aimed at testing the potential biofungicidal activity of Trichoderma harzianum in inhibiting the growth of a putatively pathogenic fungus isolated from rodent tuber (Typhonium flagelliforme) plant. The methods consisted of isolation of fungi from the plant’s surface, soil, and polybags. The isolates were then screened for their putative pathogenicity against rodent tuber before being subjected to 16S rRNA molecular identification and in vitro antagonist test using T. harzianum. Result showed that only isolate K4 showed pathogenicity on T. flagelliforme, and was molecularly identified as Lasiodiplodia theobromae, known globally as fungal pathogen attacking various plants. L. theobromae was inhibited by T. harzianum with inhibition index of 23.0 ± 4.3%, which was about twice higher than that of the positive control nystatin 100.000 IU mL (11.1 ± 0.6%). In conclusion, T. harzianum inhibited the growth of L. theobromae in vitro, hence indicating its biofungicidal potential.  Trichoderma spp. merupakan biofungisida paling dominan di pasar global. Kemampuan pengendalian menggunakan Trichoderma spp. terhadap kapang patogen pada tanaman komoditas pangan sudah banyak dilaporkan, namun belum banyak untuk tanaman obat. Penelitian ini bertujuan menguji potensi aktivitas biofungisida Trichoderma harzianum dalam menghambat pertumbuhan kapang terduga patogen yang diisolasi dari tanaman keladi tikus (Typhonium flagelliforme). Metode penelitian meliputi isolasi kapang dari permukaan tanaman, tanah, dan polibag. Penapisan dilakukan untuk mendapatkan isolat yang berpotensi patogen terhadap keladi tikus, untuk kemudian diidentifikasi secara molekuler menggunakan 16S rRNA dan diuji antagonis in vitro menggunakan T. harzianum. Hasil penelitian menunjukkan bahwa hanya isolat K4 yang bersifat patogen pada T. flagelliforme dan secara molekuler diidentifikasi sebagai Lasiodiplodia theobromae, yang dikenal sebagai jamur patogen yang menyerang berbagai tanaman. Pertumbuhan L. theobromae dihambat oleh T. harzianum dengan indeks penghambatan 23,0 ± 4,3%, atau  dua kali lebih tinggi dari kontrol positif nistatin 100.000 IU mL (11,1 ± 0,6%). Sebagai kesimpulan, T. harzianum menghambat pertumbuhan L. theobromae secara in vitro, yang menunjukkan potensi biofungisidanya.
OPTIMIZATION OF Agrobacterium-MEDIATED GENETIC TRANSFORMATION OF oshox4 GENE IN SATOIMO TARO (Colocasia esculenta var. antiquorum) Febrina Ariyanti Ismail; Aris Tjahjoleksono; N Sri Hartati
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022): June 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (447.43 KB) | DOI: 10.29122/jbbi.v9i1.3063

Abstract

Satoimo (Colocasia esculenta var. antiquorum) can be considered as an alternative food to support food diversification. The aim of this research was to obtain an optimum condition of the genetic transformation method of Satoimo taro through Agrobacterium-mediated genetic transformation using binary vector harboring gene construct of oshox4 and hpt gene as a selectable marker to produce transgenic plants. Plant materials that were used in this study were meristem, root, leaf, petiole, and basal stem. Transformation procedures were carried out using three different co-cultivation periods (1, 2, and 3 days) and different optical densities of A. tumefaciens cells (0,5 and 0,7). Furthermore, callus induction was performed on MS medium containing 2.4-D, cefotaxime, hygromycin and then regenerated on MS medium containing thidiazuron and hygromycin. The putative transgenic plantlets were selected on MS medium containing hygromycin and analyzed using PCR. The results showed that putative transgenic plants derived from the basal stem could survive on a selection medium containing hygromycin and based on PCR analysis some of them contained the oshox4 and hpt genes. Satoimo (Colocasia esculenta var. antiquorum) dapat dijadikan sebagai alternatif pangan untuk mendukung penganekaragaman pangan. Tujuan penelitian ini adalah mendapatkan kondisi optimal untuk transformasi genetik talas Satoimo menggunakan gen oshox4 yang dimediasi oleh Agrobacterium tumefaciens. Transformasi genetik dilakukan dengan menggunakan vektor biner yang mengandung gen oshox4 dan gen hpt. Bahan tanaman yang digunakan dalam penelitian ini adalah meristem, akar, daun, tangkai daun dan pangkal batang talas Satoimo. Transformasi dilakukan dengan menggunakan tiga perlakuan waktu ko-kultivasi (1, 2, dan 3 hari) serta dua optical density (0,5 dan 0,7). Selanjutnya dilakukan induksi kalus pada media MS yang mengandung 2.4-D, cefotaksim, hogromisin dan diregenerasikan pada media MS yang mengandung thidiazuron dan higromisin. Plantlet putatif transgenik diseleksi menggunakan media MS yang mengandung higromisin serta dianalisis menggunakan PCR. Hasil penelitian menunjukkan bahwa tanaman putatif transgenik yang berasal dari pangkal batang dapat bertahan hidup pada media seleksi yang mengandung higromisin dan berdasarkan analisis PCR, beberapa di antaranya mengandung gen oshox4 dan gen hpt.
MICROBIAL BIOCONVERSION TO PRODUCE NUTRACEUTICAL AND PHARMACEUTICAL BIOACTIVE COMPOUNDS Siti Mahyuni
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022): June 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (336.623 KB) | DOI: 10.29122/jbbi.v9i1.4754

Abstract

The bioconversions of bioactive health supporting compounds using certain microorganisms have long been known, however it has not been widely applied in industrial scale to produce nutraceutical and products of high economic value. This review article will discuss the group of steroids, carotenoids and statins which were produced by microbial bioconversion using the various substrates and conditions. The information presented in this article were collected from scientific books and articles in national and international journals which can be validated and traced back to their sources through many sites such as google.com, google scholar, NCBI and Science Direct. From the information compiled in this article, it can be concluded that the bioconversion of bioactive compounds by microorganisms has shown positive results and potential to be further optimized and applied in an industrial scale to produce natural bioactive compounds as basic ingredients for nutraceutical and pharmaceutical products with high economic value. Senyawa-senyawa bioaktif penunjang kesehatan hasil biokonversi oleh mikroorganisma telah lama dikenal namun dimanfaatkan secara maksimal pada industri nutraseutik dan farmaseutik untuk menghasilkan produk-produk bernilai ekonomi tinggi. Pada artikel ini akan ditinjau produk-produk nutraseutikal dan farmaseutikal dari golongan steroid, golongan karetinoid, dan golongan statin yang dihasilkan dari proses biokonversi oleh berbagai mikroorganime. Informasi-informasi yang disajikan didapatkan dengan metoda studi lieratur mengacu pada berbagai tulisan ilmiah nasional dan internasuonal yang dapat divalidasi dan dilacak kembali sumbernya melalui situs-situs google.com, google scholar, NCBI dan Science Direct. Dari artikel tinjauan ini dapat disimpulkan bahwa biokonversi senyawa bioaktif oleh mikroorganisma menunjukkan hasil-hasil positif dan berpotensi untuk dioptimasi dan diterapkan lebih lanjut pada skala industri untuk menghasilkan senyawa-senyawa sebagai bahan dasar produk nutraseutik dan farmasetik yang bernilai ekonomi tinggi.
SEGREGATION ANALYSIS OF MORPHOLOGICAL CHARACTER AND BIOACTIVE GENOTYPE OF RICE PLANT F3 (CEMPO SALAMET/IR64) POPULATION Indri Gita Lestari; Mohammad Ubaidillah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022): June 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (409.844 KB) | DOI: 10.29122/jbbi.v9i1.4859

Abstract

Red rice contains high anthocyanin and bioactive antioxidant compounds that prevent free radical reactions. Cempo Salamet has potential as an antioxidant source, and the characteristics are red colored grains, 4–5 months old, 169 cm plant height, 7 productive tillers per plant, and resistance to blast disease. IR64 had been developed with the following characteristics: 3 months old, 85 cm plant height, 20–35 productive tillers per plant, resistance to brown leafhoppers pigment. This study aimed to obtain information on the segregation of the F3 population from crosses between the Cempo Salamet and IR64 varieties. Research methods included preparation and maintenance with genotype analysis. PCR analysis was conducted using SSR markers with primer RM346, RM316, RM228, and RM339. The segregation in F3 plants was 50% for >130 cm plant height, 51% for 10–19 tillers per plant, 67% for 2.2 g/100-grain weight, and 33% strong red for colour intensity. The findings demonstrated that SSR markers RM346, RM339, and RM228 could validate Cempo Salamet, IR64, and F3 DNA bands. However, RM316 could not validate all DNA bands in the research sample. Beras merah mengandung antosianin yang tinggi dan senyawa bioaktif antioksidan yang mampu mencegah terjadinya reaksi radikal bebas. Cempo Salamet berpotensi sebagai sumber antioksidan yang memiliki karakteristik biji berwarna merah, umur 4–5 bulan, tinggi tanaman 169 cm, anakan produktif 7 batang, tahan terhadap penyakit blas. IR64 telah banyak dibudidayakan dengan karakteristik umur 3 bulan, tinggi tanaman 85 cm, anakan produktif 20–35 batang, tahan terhadap wereng coklat, akan tetapi tidak mengandung pigmen. Penelitian ini bertujuan mendapatkan informasi segregasi populasi F3 hasil persilangan antara varietas Cempo Salamet dan IR64. Metode penelitian meliputi persiapan dan pemeliharaan tanaman serta analisis genotipe bioaktif. Analisis PCR menggunaan marka SSR dengan primer RM 346, RM 316, RM 228, dan RM 339. Terjadi segregasi karakter morfologi pada populasi F3 yaitu diperoleh tanaman dengan tinggi >130 cm (50%), 10–19 anakan (51%), bobot 100 bulir dengan 2,2 g (67%), dan intensitas warna bulir dengan merah kuat (33%). Penelitian menunjukkan marker SSR yang dapat memvalidasi pita-pita DNA yaitu RM346, RM339, and RM228, sedangkan RM316 tidak dapat memvalidasi keseluruhan pita-pita DNA pada sampel penelitian.
THE APPLICATION OF Fe AND Cr(III) IN GROWING MEDIA AND ITS EFFECT ON PLANT GROWTH AND Cr(III) OXIDATION ON Tagetes erecta Penina Intansari; Sri Kasmiyati; . Sucahyo
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022): June 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (766.085 KB) | DOI: 10.29122/jbbi.v9i1.4865

Abstract

The oxidation of Cr(III) to Cr(VI) in the environment has a detrimental impact because it can change the form of non-toxic Cr(III) to Cr(VI), which is toxic to organisms. The study aimed to examine the effect of the application of iron (Fe) and trivalent chromium (Cr(III)) compounds in Tagetes erecta growing media on growth and Cr(III) oxidation. Concentrations of Cr(III) 0, 100, and 500 mg/L and Fe 0, 3, 15, and 30 mg/L were applied to the growing media of T. erecta as the model plant. The growth and accumulation of Cr(VI) in plants were measured to determine the effect of Fe and Cr(III) treatment on growth and Cr(III) oxidation. The accumulation of Cr(VI) in the roots and shoots of T. erecta increased significantly due to the addition of Fe in the growing media treated with Cr(III). The highest accumulation of Cr(VI) in the roots and shoots of T. erecta found in the treatment of Cr(III) 500 mg/L and Fe 30 mg/L, were respectively 0.092 g/L and 0.070 g/L. The addition of Fe in growing media containing Cr(III) increased plant height, root length, and shoot dry weight but decreased leaf number and root dry weight. T. erecta root biomass was more affected by the toxic impact of Fe than Cr(III). On the other hand, the inhibition of leaf formation was caused by the toxic effect of Cr(III) rather than Fe. Oksidasi Cr(III) menjadi Cr(VI) di lingkungan mempunyai dampak merugikan, karena dapat mengubah bentuk Cr(III) dari tidak toksik menjadi bentuk Cr(VI) yang toksik bagi organisme. Tujuan penelitian adalah untuk mengkaji efek aplikasi senyawa besi (Fe) dan kromium trivalen (Cr(III)) dalam media tanam Tagetes erecta terhadap pertumbuhan dan oksidasi Cr(III). Konsentrasi Cr(III) 0, 100 dan 500 mg/L dan Fe 0, 3, 15 dan 30 mg/L diaplikasikan dalam media tanam T. erecta sebagai tanaman uji. Pertumbuhan dan akumulasi Cr(VI) pada tanaman diukur untuk mengetahui efek perlakuan dan mendeteksi terjadinya oksidasi Cr(III). Akumulasi Cr(VI) pada akar dan pucuk T. erecta mengalami peningkatan secara nyata akibat penambahan Fe dalam media tanam yang diberi perlakuan Cr(III).  Akumulasi Cr(VI) pada akar dan pucuk T. erecta tertinggi dijumpai pada perlakuan Cr(III) 500 mg/L dan Fe 30 mg/L berturut-turut adalah 0,092 g/L dan 0,070 g/L. Penambahan Fe dalam media tanam mengandung Cr(III) meningkatkan pertumbuhan tinggi tanaman, panjang akar dan berat kering pucuk, namun menurunkan jumlah daun dan berat kering akar. Biomassa akar T. erecta lebih dipengaruhi oleh efek toksik Fe dibandingkan Cr(III), sebaliknya penghambatan pembentukan daun lebih disebabkan oleh efek toksik Cr(III) daripada Fe.
MOLECULAR IDENTIFICATION AND MORPHOLOGICAL CHARACTERIZATION OF PATCHOULI (Pogostemon sp.) FROM BATANG REGENCY, CENTRAL JAVA PROVINCE Aeldo Yudifian; Anto Budiharjo; Rejeki Siti Ferniah; Hermin Pancasakti Kusumaningrum
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022): June 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (378.961 KB) | DOI: 10.29122/jbbi.v9i1.4884

Abstract

Patchouli (Pogostemon cablin Benth.) is an essential oil-producing plant from Batang Regency that has excellence in patchouli alcohol contents and highly survives in any condition. Molecular identification has been done in the ITS region because DNA sequences in the ITS rRNA region evolved faster than in other areas. This study aimed to get the molecular and morphological identity of patchouli (Pogostemon sp.) from BPP Kabupaten Batang. The study consisted of sample preparation, DNA isolation, amplification, electrophoresis, sequence data analysis, and phylogenetic analysis using MEGA X. All parts of plant were morphologically identified and compared with patchouli from Sidikalang, Aceh, Java, and China. Extraction DNA produced 301.7 ng ?L–1 concentration and 1.93 purity. Amplification of ITS fragment patchouli produced a 670 bp-sized single band. Phylogenetic analysis showed patchouli BPP related to Pogostemon cablin (KR608752.1) with 98% coverage identity. BPP patchouli showed 62,5% morphological similarity with Sidikalang patchouli compared to Java patchouli. In conclusion, BPP patchouli is a Sidakalang patchouli P. cablin that has undergone environmental adaptation. Nilam (Pogostemon cablin Benth.) adalah tanaman atsiri dari Kabupaten Batang dengan keunggulan berupa kandungan patchouli alcohol dan ketahanan hidup yang tinggi. Identifikasi molekuler dilakukan pada daerah ITS karena sekuens DNA pada daerah ITS rRNA berevolusi lebih cepat dibandingkan daerah gen lain sehingga akan bervariasi pada setiap spesies. Penelitian ini bertujuan memperoleh identitas molekuler dan morfologi tanaman nilam (Pogostemon sp.) dari BPP Kabupaten Batang. Penelitian ini terdiri dari penyiapan sampel, isolasi DNA, amplifikasi fragmen ITS, elektroforesis, sekuensing, analisis data sekuens, analisis filogenetik menggunakan MEGA X. Morfologi seluruh bagian tanaman diidentifikasi dan dibandingkan dengan nilam dari Sidikalang Aceh, Jawa, dan Cina. Isolasi DNA nilam menghasilkan konsentrasi 301,7 ng µL–1 dan kemurnian 1,93. Amplifikasi fragmen ITS nilam menghasilkan pita tunggal 670 bp. Hasil identifikasi molekuler diikuti dengan analisis filogenetik menunjukkan nilam BPP Kabupaten Batang berkerabat dekat dengan P. cablin (KR608752.1) sebesar 98%. Hasil identifikasi morfologis nilam BPP memperlihatkan 62,5% persamaan morfologi dengan nilam Sidakalang dibandingkan dengan nilam Jawa. Kesimpulan, nilam BPP merupakan nilam Sidakalang P. cablin yang telah mengalami adaptasi lingkungan.
MITOCHONDRIAL DNA DIVERSITY IN FOUR POPULATIONS OF INDONESIAN FRESHWATER GIANT PRAWN (Macrobrachium rosenbergii) Ratu Siti Aliah; Sutanti; Fetrilisa Silitonga
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022): June 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (489.034 KB) | DOI: 10.29122/jbbi.v9i1.4921

Abstract

Mitochondrial DNA has been widely applied to analyze fish genetic diversity, especially the cytochrome oxidase subunit I (COI) gene, which can be used as a marker of typical variation patterns, both intraspecific and interspecific crossbreeding. Haplotype diversity was observed in four populations of giant freshwater prawns (Macrobrachium rosenbergii) originating from Peureulak River in Aceh, Tabuk River in South Kalimantan, Bengawan Solo River in East Java, and the Siratu strain. Haplotype diversity was observed in the mitochondrial DNA COI gene of 1516 bp and restricted using 5 enzymes, namely AvaII, MspI, RsaI, HaeIII, and TaqI. The diversity of mtDNA COI haplotypes in the population of Peureulak River, Tabuk River, Bengawan Solo River, and Siratu were 0.8183, 0.6222, 0.7461, and 0.8044, respectively. The dendrogram of genetic distance showed that the Bengawan Solo River populations and the Siratu strain were in the same cluster, while Peureulak River and Tabuk River populations were in another group. The four populations can be used as genetic sources in crossbreeding activities based on haplotype data for each population, haplotype diversity, and genetic distance. DNA Mitokondria telah banyak diaplikasikan untuk analisa keragaman genetik ikan, terutama gen cytochrome oxidase subunit I (COI) yang dapat digunakan sebagai penanda pola variasi yang khas baik pada persilangan intraspesifik maupun interspesifik. Keragaman haplotipe diamati pada empat populasi udang galah (Macrobrachium rosenbergii) yang berasal dari Sungai Peureulak di Aceh, Sungai Tabuk di Kalimantan Selatan, Sungai Bengawan Solo di Jawa Timur dan strain Siratu. Keragaman haplotipe diamati pada gen COI DNA mitokondria yang berukuran 1516 bp dan direstriksi menggunakan 5 enzim, yaitu AvaII, MspI, RsaI, HaeIII, dan TaqI. Keragaman haplotipe mtDNA COI pada populasi Sungai Peureulak, Sungai Tabuk, Sungai Bengawan Solo dan Siratu masing-masing adalah 0,8183; 0,6222; 0,7461 dan 0,8044. Dendrogram jarak genetik menunjukkan bahwa populasi Sungai Bengawan Solo dan galur Siratu berada dalam 1 kluster yang sama, sedangkan populasi Sungai Peureulak dan Sungai Tabuk berada pada kluster lainnya. Berdasarkan data haplotipe untuk setiap populasi, keragaman haplotipe dan jarak genetik keempat populasi tersebut dapat dijadikan sebagai sumber genetik dalam kegiatan persilangan.
ETHANOL EXTRACT OF Moringa oleifera INCREASED THE NUMBER OF SPERMATOZOA AND IMPROVED SPERM MORPHOLOGY OF OLD Rattus norvegicus Luh Putu Widiastini; I Gusti Agung Manik Karuniadi; Made Tangkas
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022): June 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (403.426 KB) | DOI: 10.29122/jbbi.v9i1.5021

Abstract

Aging in men can lead to decreased semen volume, abnormal sperm morphology, and decreased sperm motility. This study aims to determine the effect of ethanol extract of moringa (Moringa oleifera) leaf on the number and morphology of spermatozoa in old Wistar rats (Rattus norvegicus). This study used healthy old rats, 18–19 months old, with a bodyweight of 200–250 g and no physical disabilities. The 36 rats were divided into 2 groups, i.e. the treatment group (fed with ethanol extract of moringa leaves 50 mg/kgBW/0.5 mL CMC 0.5% every day) and the control group (0.5 mL CMC 0.5% every day) for 30 days. The results showed that moringa leaf ethanol extract increased the number of spermatozoa (p-value 0.000) and improved spermatozoa morphology (p-value 0.000). It can be concluded that the ethanol extract of moringa leaves significantly increased the number and improved the morphology of the spermatozoa in the aged rats.  Penuaan pada pria dapat menyebabkan penurunan volume semen, morfologi yang abnormal dan penurunan motilitas sperma. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh pemberian ekstrak etanol daun kelor (Moringa oleifera) terhadap jumlah serta morfologi spermatozoa tikus putih (Rattus norvegicus) galur Wistar usia tua. Penelitian ini menggunakan tikus tua yang berusia 18–19 bulan dengan berat badan 200–250 g, kondisi sehat dan tidak cacat fisik. Sebanyak 36 ekor tikus dibagi menjadi 2 kelompok, yaitu kelompok perlakuan (ekstrak etanol daun kelor 50 mg/kgBB/0,5 mL CMC 0,5% per hari) dan kelompok kontrol (CMC 0,5% 0,5 mL per hari) selama 30 hari. Hasil penelitian menunjukkan bahwa ekstrak etanol daun kelor meningkatkan jumlah spermatozoa (nilai p 0,000) dan memperbaiki morfologi spermatozoa (nilai p 0,000). Dapat disimpulkan bahwa pemberian ekstrak etanol daun kelor secara signifikan meningkatkan jumlah dan kualitas morfologi spermatozoa tikus putih usia tua.
RESPONSE OF SEED GERMINATION AND GROWTH OF Nepenthes gymnamphora Nees IN VITRO TO THE CONCENTRATION OF MS MINERAL SALT, PEPTONE AND THIDIAZURON Fella Suffah Meinaswati; Nintya Setiari; Yulita Nurchayati; Sri Widodo Agung Suedy
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022): June 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (346.874 KB) | DOI: 10.29122/jbbi.v9i1.5049

Abstract

Nepenthes gymnamphora Nees is a Java's rare endemic species. Ex-situ conservation of this endangered species can be done through in vitro culture technique. The aims of this study were to determine (1) the mineral salt concentration of MS basal media and addition of peptone (P) on N. gymnamphora seed germination and seedling emergence and (2) the effects of TDZ in ½MS medium on seedling growth. Seeds were surface sterilized and cultured on four media formulations (½MS, MS, ½MS+P, MS+P) for 8 weeks. In the second experiment, ten-week-old seedlings, 0,25 cm in length were cultured on ½MS supplemented with 0, 0,5, 1,0, or 1,5 mg/L TDZ. Seedling growth was recorded at 8 weeks of culture. Results of this experiment showed that ½MS was the best medium for N. gymnamphora seed germination as indicated by the highest percentage of germination, the tallest seedling, and the fastest seedling emergence. Moreover, the best growth of N. gymnamphora was found on ½MS without TDZ. Nepenthes gymnamphora Nees. merupakan spesies endemik Pulau Jawa yang tergolong langka, sehingga perlu upaya konservasi. Konservasi ex situ spesies ini dapat dilakukan dengan teknik kultur jaringan. Penelitian ini bertujuan untuk mengetahui konsentrasi garam mineral media MS dan pepton yang dapat mendukung perkecambahan biji dan menentukan konsentrasi TDZ untuk pertumbuhan kecambah N. gymnamphora in vitro.  Pada percobaan I, biji N. gymnamphora disterilisasi dan ditabur di 4 kombinasi media, yaitu MS, ½MS, dengan dan tanpa penambahan 2g/L pepton. Pada percobaan II, kecambah berukuran ± 0,25 cm dengan penambahan beberapa konsentrasi TDZ (0; 0,5; 1; 1,5 ppm) pada media ½MS. Hasil penelitian menunjukkan bahwa media ½MS menghasilkan persentase perkecambahan biji tertinggi (56%) dengan tinggi kecambah kecambah terbaik. Media ½MS tanpa TDZ menghasilkan pertumbuhan kecambah terbaik yang ditunjukkan oleh waktu tercepat munculnya daun, Media ½MS merupakan konsentrasi garam mineral terbaik untuk perkecambahan biji N. gymnamphora, tanpa TDZ.
ANALYSIS OF BBM, LEC, AND SERK EXPRESSIONS IN CALLUS OF SUGARCANE (Saccharum officinarum L.) AT SOMATIC EMBRYOGENESIS DEVELOPMENT STAGES Lisa Prastuti Anggraeni; Firdha Narulita Alfian; Laily Ilman Widuri; Parawita Dewanti
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022): June 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (459.997 KB) | DOI: 10.29122/jbbi.v9i1.5223

Abstract

Callus formed in somatic embryogenesis is divided into two types, embryogenic and non-embryogenic callus. Embryogenic callus can be distinguished by the expression of genes as markers related to somatic embryogenesis which are expected to be used as markers to detect callus that has embryogenic capabilities in sugarcane. The aim of this study was to determine the expressions of somatic embryogenesis-related genes in sugarcane. The genes analysis was carried out using somatic embryogenesis callus and using a kit for RNA analysis. Results showed that there were expressions of Baby Boom (BBM), Leafy Cotyledon (LEC), dan Somatic Embryogenesis Receptor Like-Kinase (SERK) gene with specific primer of those three genes, which were collected from embryogenic callus in mass, globular, scutelar, and coleoptilar pre-embryo stages, meanwhile there were no expressions of those genes collected from non-embryogenic callus. Amplification result from PCR product of cDNA using three gene primers detected were in 500 bp for BBM, 400 bp for LEC, and 700 bp for SERK gene. Kalus yang terbentuk pada embriogenesis somatik terbagi menjadi 2 jenis, yatu kalus embriogenik dan non embriogenik. Kalus embriogenik dapat dibedakan dengan adanya ekspresi gen sebagai penanda karena adanya proses somatik embriogenesis yang nantinya diharapkan dapat digunakan sebagai marka untuk mendeteksi kalus yang memiliki kemampuan embriogenik pada tanaman tebu. Tujuan dari penelitian ini yaitu untuk mengetahui ekspresi gen yang diduga terkait proses embriogenesis somatik pada tanaman tebu. Analisis gen terkait proses embriogenesis tersebut dilakukan dengan menggunakan kalus hasil embriogenesis somatik, dan penggunaan kit untuk analisis RNA. Hasil penelitian menujukkan bahwa terdapat ekspresi gen Baby Boom (BBM), Leafy Cotyledon (LEC), dan Somatic Embryogenesis Receptor Like-Kinase (SERK) dengan primer spesifik ketiga gen tersebut, yang diambil dari sampel kalus embriogenik fase pre-embryo mass, globular, skutelar, dan koleoptilar, namun tidak adanya ekspresi ketiga gen tersebut pada kalus non embriogenik. Hasil amplifikasi sampel produk PCR dari cDNA menggunakan ketiga primer gen yang terdeteksi memiliki ukuran sebesar 500 bp untuk gen BBM, 400 bp untuk gen LEC, dan 700 bp untuk gen SERK.

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