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Medical Laboratory Technology Journal
Published by Poltekkes Kemenkes Banjarmasin
ISSN : -     EISSN : 24610879     DOI : https://doi.org/10.31964/mltj
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Nursing Public Health
The aim of this journal publication is to disseminate the conceptual thoughts or ideas and research results that have achieved in the area of medical laboratory sciences. This Journal particularly focuses on the main problems in the development of the sciences of medical laboratory areas. It covers the parasitology, bacteriology, virology, hematology, clinical chemistry, toxicology, food and drink chemistry, and any sciences that cover sciences of medical laboratory area.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 11 Documents
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Search results for , issue "vol. 12 no. 1 (2026): june" : 11 Documents clear
Correlatıon of Plaque Characterıstıcs wıth Degree of Stenosıs Evaluated by Coronary Angıography Usıng Computed Tomography Kurnia Arma Wijayanti; Anggraeni Dwi Sensusiati; Anita Widyoningroem
Medical Laboratory Technology Journal Vol. 12 No. 1 (2026): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v12i1.651

Abstract

Coronary artery disease remains the leading cause of global mortality, and the diagnosis of acute chest pain continues to pose a challenge in emergency care due to the limitations of conventional methods in detecting acute coronary syndrome. Coronary computed tomography angiography (CCTA) has emerged as an efficient non-invasive diagnostic tool for evaluating coronary artery plaque characteristics and stenosis severity. This retrospective study analyzed the correlation between plaque types and degrees of stenosis detected by CCTA in 54 patients presenting with chest pain at Dr. Soetomo General Hospital, Surabaya, during January–December 2023. Plaque morphology was classified into calcified, non-calcified, and mixed types based on CAD-RADS 2.0 criteria. Data were analyzed using Spearman’s correlation test. Mixed plaques were the most frequently observed (120 samples), followed by calcified (78 samples) and non-calcified (71 samples). Moderate stenosis was most commonly associated with mixed plaques, while severe stenosis was most frequently found in association with non-calcified plaques. Statistical analysis revealed a significant and very strong correlation between plaque type and stenosis severity in eight coronary segments. These findings highlight the diagnostic value of CCTA in evaluating plaque morphology and stenosis, particularly in identifying mixed plaques that are closely linked to more severe coronary obstruction. The results support the role of CCTA as a critical tool in the diagnostic workup of patients with acute chest pain.
Association between D-Dimer and CRP Levels in Confirmed COVID-19 Patients with Diabetes Mellitus Comorbidity at Jakarta Hajj Hospital Atna Permana; Adik Purnama; Warida Warida; Haitami Haitami
Medical Laboratory Technology Journal Vol. 12 No. 1 (2026): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v12i1.658

Abstract

COVID-19 may induce systemic inflammation and coagulation abnormalities, particularly in patients with Diabetes Mellitus (DM). C-Reactive Protein (CRP) is an inflammatory biomarker, while D-Dimer reflects coagulation activation and fibrinolysis. In COVID-19 patients with DM, both biomarkers may increase due to chronic inflammation, hyperglycemia, endothelial dysfunction, and a prothrombotic state. This study aimed to analyze the correlation between D-Dimer and CRP levels in confirmed COVID-19 patients with comorbid Diabetes Mellitus at Haji Hospital Jakarta. This was an observational analytic study with a cross-sectional design using secondary data from medical records of hospitalized COVID-19 patients with DM at Haji Hospital Jakarta from June to September 2021. Total sampling was applied. A total of 80 patients who met the inclusion and exclusion criteria were included in the analysis. The main variables were D-Dimer and CRP levels. Descriptive analysis was used to describe subject characteristics, while the Spearman correlation test was used to assess the correlation between D-Dimer and CRP because the data were not normally distributed. Among 80 subjects, 44 patients (55.0%) were male, and 36 patients (45.0%) were female. The mean age was 55.76 ± 12.28 years, and the mean random blood glucose level was 303.75 ± 70.56 mg/dL. The mean D-Dimer level was 0.96 ± 1.51 µg/mL with a median of 0.50 µg/mL, while the mean CRP level was 58.48 ± 28.59 mg/L with a median of 55.0 mg/L. Elevated D-Dimer levels were found in 41 patients (51.3%), while all subjects (100%) had elevated CRP levels. Spearman correlation analysis showed no significant correlation between D-Dimer and CRP levels (r_s = -0.057; p = 0.618). There was no significant correlation between D-Dimer and CRP levels in COVID-19 patients with comorbid Diabetes Mellitus at Haji Hospital Jakarta. However, both biomarkers may still provide complementary information regarding inflammatory and coagulation status abnormalities in COVID-19 patients with DM.
Tube-Tests of Eichhornia crassipes and Pistia stratiotes Extracts as Bacterial Anti-Biofilm Lia Yulia Budiarti; Farida Heriyani; Shofia Hilwa Ihsanti; Fadya Rahma Sari; Yogi Waskito Aji; Maulidia Khairada Amalia
Medical Laboratory Technology Journal Vol. 12 No. 1 (2026): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v12i1.673

Abstract

Bacterial biofilms have strong adhesion to medical devices and are resistant to disinfectants, potentially triggering nosocomial infections. One promising approach involves the use of alternative phytochemical-based disinfectants. Eichhornia crassipes (E. crassipes) and Pistia stratiotes (P. stratoites) plants are known to possess antibacterial compounds; however, their anti-biofilm potential remains to be studied. This experimental study aimed to assess the antibiofilm potential of ethanol extracts of E.crassipes and P.stratiotes using a test tube method. The combination extract (EC+PS) was prepared at concentrations of 6.25% - 100% and tested in triplicate along with a control. Its ability was tested on three ATCC bacterial strains (S. aureus, E. coli, and P.aeruginosa) that form biofilms; confirmed by the growth of black colonies on Congo Red Agar (CRA). Qualitative observations determined the Minimum Biofilm Inhibitory Concentration (MBIC), revealing effective inhibition of 12.5% ​​for E. coli and S. aureus, and 25% for P. aeruginosa. ANOVA analysis of biofilm intensity based on the Mean Gray Value (MGV) parameter showed significant variation between the EC+PS treatment and the control group (p < 0.05). Duncan's post hoc test showed the antibiofilm effect of EC+PS was comparable to chlorine: 75% for S. aureus and 100% for E. coli and P. aeruginosa (p < 0.05). In conclusion, the anti-biofilm activity of E. crassipes and P. stratoites shows promise as alternative disinfectants.
The Effect of Periwinkle Flower Extract Gel (Catharanthus roseus) on MITF and GPx in C57BL/6 Mice Exposed to Sub-chronic UVB Lidya Wulandari; Hadi Sarosa; Joko Wahyu Wibowo
Medical Laboratory Technology Journal Vol. 12 No. 1 (2026): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v12i1.702

Abstract

Ultraviolet B (UVB) exposure induces oxidative stress, which decreases antioxidant enzyme activity such as Glutathione Peroxidase (GPx) and activates the transcription factor Microphthalmia-associated Transcription Factor (MITF), a key regulator of melanocyte survival and melanogenic adaptation. Natural antioxidants such as Catharanthus roseus (Madagascar periwinkle) extract possess the potential to enhance endogenous antioxidant defences and modulate UVB-induced melanocyte responses. This study tries to determine the effect of Catharanthus roseus flower extract gel on MITF expression and GPx levels in the skin tissue and GPx levels in the serum of C57BL/6 mice exposed to subchronic UVB radiation. This experimental study employed a post-test only control group design with five groups: K1 (normal), K2 (negative control, gel base), K3 (positive control, vitamin E), K4 (Catharanthus roseus Extract gel 15%), and K5 (Catharanthus roseus Extract gel 30%). The mean GPx levels differed significantly (p=0.001): K1=10.66±0.85 ng/mL, K2=2.60±1.22 ng/mL, K3=3.70±1.00 ng/mL, K4=8.29±1.95 ng/mL, and K5=9.15±0.91 ng/mL. MITF expression also showed significant differences (p=0.003): K1=27.47±3.62%, K2=31.82±8.11%, K3=51.06±8.16%, K4=34.49±7.91%, and K5=40.49±3.74%. Based on these observations, it can be inferred that the administration of Catharanthus roseus flower extract gel significantly increased GPx levels and modulated MITF expression in UVB-exposed mouse skin. The 30% concentration demonstrated the most optimal antioxidant and protective effects, approaching physiological antioxidant levels, while maintaining controlled MITF activation, highlighting its potential as a natural photoprotective and antioxidant agent.
Antioxidant, Anti-Inflammatory, and Antibacterial Activities and Phytochemical Content of White Champaca (Michelia alba) Flower Extract I Nyoman Jirna; I Nyoman Gede Suyasa; Cok Dewi Widya Hana Sundari; Nyoman Mastra; I Nyoman Purna Purna; Aprilia Rakhmawati
Medical Laboratory Technology Journal Vol. 12 No. 1 (2026): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v12i1.708

Abstract

Michelia alba is a member of the Magnoliaceae family known to be rich in secondary metabolites with various biological activities. Multiple studies on related plants have shown that the flowers contain flavonoids, terpenoids, and phenolics that have potential as anti-inflammatory and antibacterial agents. The objectives of this study were to evaluate the phytochemical content of Michelia alba flower extracts and to determine their anti-inflammatory and antibacterial activities through an in vitro approach. Extraction was performed using the ethanol maceration method, followed by qualitative and quantitative phytochemical tests; quantitative phytochemical testing was performed using a UV-Vis spectrophotometer. Anti-inflammatory activity was tested using a UV-Vis spectrophotometer at a wavelength of 560 nm based on the membrane stabilization method, while antibacterial activity against Staphylococcus aureus was assessed using the disk diffusion method. The results showed that the extract contained flavonoids, phenolics, tannins, and saponins qualitatively, with a total phenolic content of 80.53 mg GAE/g , total tannin content of 122.32 mg TAE/g, and a total flavonoid content of 84.14 mg QE/g. Antioxidant test showed a content of 54.64 ppm (strong), anti-inflammatory test showed a potential with an average  98.90% (very strong), and antimicrobial potential in vitro with an inhibitory power of <15 mm with a variation of 5 mm - 10.25 mm. There was no difference in various concentrations (55%, 70%, 85%, and 100%) of white Michelia alba flower extract in inhibiting the growth of S. aureus, with a sig value = 0.095. The complex metabolite profile allows for synergistic effects between compounds, so that even though the inhibition zones are moderate, the biological activity remains pharmacologically relevant. Overall, this study shows that Michelia alba flower extract has significant potential as a candidate natural anti-inflammatory and antibacterial ingredient for the development of phytochemical-based products.
Bacterial Profile and Multiplex PCR Detection of SHV, CTX-M, and mecA Genes in Diabetic Ulcer Infections Amellya Octifani; Siti Nur Azizah Binti Fandik; Yulianto Ade Prasetya
Medical Laboratory Technology Journal Vol. 12 No. 1 (2026): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v12i1.716

Abstract

Diabetes mellitus is a chronic disease with complications such as diabetic ulcers, which can lead to amputation. High blood glucose facilitates a mix of aerobic and anaerobic bacterial growth in ulcers. Prolonged antibiotic use risks resistance. This study aims to identify bacterial species and the presence of resistance genes (SHV, CTX-M, and mecA) in isolates from diabetic ulcer patients at Wahidin Hospital, Mojokerto, using multiplex PCR. The study design was a descriptive, observational study to identify bacterial patterns and detect the presence of SHV, CTX-M, and mecA genes in bacterial samples from ulcers of diabetic patients using a multiplex molecular genetic method. Bacterial identification of 32 ulcer swab samples from DM patients revealed Staphylococcus aureus (63%), Escherichia coli (28%), and Klebsiella pneumoniae (9%). The results of the detection of multiplexed genes encoding resistance in the identified bacteria were SHV 3%, CTX-M 19%, mecA 22%, SHV & mecA 3%, and CTX-M & mecA 3%. The multiplex PCR method was successfully used to detect genes encoding antibiotic resistance in bacteria infecting ulcers of patients with DM.
Antioxidant Activity and Sun Protection Factor (SPF) of Various Fractions of Ethanol Extract of Kelakai Roots (Stenochlaena palustris Bedd) Muhammad Ikhwan Rizki; Anna Khumaira Sari; Satrio Wibowo Rahmatullah
Medical Laboratory Technology Journal Vol. 12 No. 1 (2026): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v12i1.738

Abstract

Excessive sun exposure in tropical regions can cause oxidative stress that triggers premature aging and skin cancer. Kelakai root (Stenochlaena palustris Bedd.) is a potential natural ingredient that contains various secondary metabolites with the ability as an antioxidant and sunscreen properties. This research aims to determine the antioxidant capacity and Sun Protection Factor (SPF) value of various fractions of ethanol extract of kelakai roots. Kelakai roots are washed with clean water, then dried in a drying cabinet, and then powdered. Extraction is carried out using ethanol as a solvent using a maceration method. The extract was fractionated using n-hexane, ethyl acetate, and aqueous. Phytochemical screening was performed using specific reagents. Antioxidant capacity was determined using the DPPH method based on the Inhibitory Concentration 50 (IC50) parameter. The ability as a sunscreen is measured based on the Sun Protection Factor (SPF) parameter with a UV-Vis spectrophotometer. The results of the identification of compound groups showed that the ethyl acetate fraction contained phenolic, flavonoid, tannin, alkaloid, and saponin compounds. Antioxidant testing showed that the ethyl acetate fraction had an IC50 of 16.67 ppm (very strong), followed by the aqueous fraction IC50 of 73.92 ppm (strong) and the n-hexane fraction IC50 of 95.29 ppm (strong). At the highest concentration of 500 ppm, it is known that the n-hexane, ethyl acetate, and aqueous fractions have SPF values ​​of 12.34, 34.62, and 15.71, respectively. The aqueous fraction is classified as medium protection, while the ethyl acetate fraction is classified as high protection, and n-hexane is classified as low protection. This study concluded that the ethyl acetate fraction of kelakai roots has strong potential as a source of antioxidants and natural sunscreen agents.
The Relationship between ABO Blood Type, Knowledge Level, Behavior, and Hypertension Status in Young Adults on Campus Neni Oktiyani; Tini Elyn Herlina; Ricko Dharmadi Utama
Medical Laboratory Technology Journal Vol. 12 No. 1 (2026): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v12i1.700

Abstract

Hypertension is a public health problem increasingly found among young adults, including college students. This study aims to analyze the relationship between ABO blood type, knowledge level, behavior, and hypertension status in young adults on campus. This study used an analytical survey method with a cross-sectional design. The study population consisted of all active students in the Medical Laboratory Technology Study Program at the Poltekkes Kemenkes Banjarmasin, with a sample size of 99 respondents selected using a total sampling technique. The researchers collected data through online questionnaires and blood pressure measurements. Data analysis used the Chi-Square test with a significance level of p<0.05. The results showed that the majority of respondents were female (80.8%), had normal blood pressure (67.7%), had good knowledge (69.7%), and had good behavior (58.6%). Statistical test results showed that gender (p=0.533), ABO blood type (p=0.916), and behavior (p=0.231) were not significantly associated with hypertension status. Conversely, knowledge level was significantly associated with hypertension status (p=0.044). This study concluded that knowledge level was associated with hypertension status, while ABO blood type and behavior showed no significant association. These findings highlight the importance of improving health literacy as part of hypertension prevention efforts in young adults.
Prevalence of Anemia among Blood Donors in Benin City: Cross-Sectional Study of Iron Status and Hematological Parameters Fidelis Ohiremen Oyakhire; Adebukola Adisa Ekoh-Jolly; Babatunde Ishola Gabriel Adejumo; Samson Efenarhua; Osamwonyi Ernest Igbinovia
Medical Laboratory Technology Journal Vol. 12 No. 1 (2026): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v12i1.726

Abstract

Despite the importance of blood donation in ensuring a constant supply of blood for emergency situations and surgical procedures, frequent blood donation leads to iron depletion and anemia, posing significant health risks to donors. The study aimed to evaluate the iron status and hematological parameters among blood donors in Benin City. A cross-sectional study involving 100 blood donors was conducted across blood donation facilities in Benin City. Hematological parameters and ferritin levels were analyzed using an automated hematology analyzer (BK3100, China) and enzyme-linked immunosorbent assay (ELISA), respectively. Data were analyzed using SPSS version 26.0. Chi-square, one-way ANOVA, and Pearson correlation tests were applied, with significance set at p < 0.05. Results of the participants, 64.0% were males and 36.0% females. The self-reported prevalence of anemia was 1.0%, while all participants had hemoglobin values below WHO reference cutoffs (p<0.05). Male donors showed significantly higher hemoglobin (12.37±0.23 vs 11.05±0.17 g/dL), packed cell volume (37.43±0.60 vs 33.20±0.48%), and ferritin levels (180.23±12.79 vs 117.48±17.43 ng/mL) compared to females (p < 0.05). Conversely, white blood cell and platelet counts were significantly higher in females (p < 0.05). No significant differences were observed across age groups and donation frequencies. Blood donation frequency showed weak negative, non-significant correlations with hemoglobin, packed cell volume, and ferritin levels (p > 0.05). Conclusion Despite the prevalence found in this study, these findings underscore the need to monitor hematological parameters and ferritin levels in blood donors, particularly among women, to ensure donor health and maintain a safe and adequate blood supply.
Impact of Malaria on Hematological Profiles, Iron Indices, and G6PD Activity in Pregnant Women Fidelis Ohiremen Oyakhire; Patricia Ejenawome Dele-Ochie; Juliana Edusola Olaniyan; Valentine Ikalumhe; Babatunde Ishola Gabriel Adejumo; Samson Efenarhua; Osamwonyi Ernest Igbinovia; Emmanuel Onosetale Afeikhena; Adebukola Adisa Ekoh-Jolly
Medical Laboratory Technology Journal Vol. 12 No. 1 (2026): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v12i1.739

Abstract

The global prevalence and impact of malaria on pregnancy highlight a significant public health challenge that demands enhanced preventive measures and targeted interventions. The study aimed to assess the hematological, iron, and G6PD status among pregnant women infected with Plasmodium falciparum. We performed a comparative cross-sectional study among two hundred (200) pregnant women infected with Plasmodium falciparum, who attended Edo Specialist Hospital, Benin City, to assess the hematological, iron, and G6PD status compared with fifty (50) Plasmodium falciparum-free pregnant women as controls. Hematological parameters, iron, TIBC (total iron binding capacity), and G6PD activity were assessed using a hematology auto analyzer and spectrophotometric methods, respectively. Data obtained were analyzed using SPSS (IBM v26).  Independent t-test and one-way analysis of variance (ANOVA) were used for continuous variables, and chi-square was used for categorical variables. Pregnant women infected with Plasmodium falciparum had 8.70%, 9.42%, 14.44%, 8.1%, 20.17%, 16.72%, 11.31%, and 27.8% reduction in red blood cell, hemoglobin, iron, packed cell volume, G6PD, total iron binding capacity, monocyte, and lymphocyte levels, respectively, compared to Plasmodium falciparum-free pregnant women as controls (p<0.05). In addition, the levels of white blood cell, platelets, and neutrophils witness 15.58%, 10.85%, and 24.02% increase in pregnant women infected with Plasmodium falciparum compared with plasmodium falciparum free pregnant women as controls (p<0.05). No significant differences were observed in serum iron and G6PD levels across gestational ages (p>0.05). The findings underscore the need of regular monitoring of hematological and biochemical parameters in pregnant women infected with Plasmodium falciparum in order to enhance policy on malaria prevention and screening in endemic regions.

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