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All Journal Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Medika Tadulako Medical Laboratory Technology Journal JURNAL ANALIS LABORATORIUM MEDIK Tropical Health and Medical Research Journal Jurnal Rakat Sehat : Pengabdian Kepada Masyarakat Jurnal Karya Generasi Sehat

Neni Oktiyani

Medical Laboratory Technology Department, Poltekkes Kemenkes Banjarmasin, Indonesia

Author-ID : 2505544

Religion Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary Other

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Hubungan Dosis Tepung Gembili (Dioscorea esculenta) Dengan Tingkat Ekspresi Enzim Ampk-α2 pada Model Tikus Diabetes Melitus neni oktiyani; sunarti Sunarti; Prasetyastuti Prasetyastuti; Jujuk Anton Cahyono
Medical Laboratory Technology Journal Vol. 1 No. 1 (2015): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Alternative methods of controlling glucose levels in patients with diabetes is by type of food, either by utilizing yam flour. At the flour contained inulin and resistant starch that can activate the enzyme AMPK-α2. Activation of these enzymes will stimulate glucose transport in skeletal muscle and liver, thus causing a decrease in glucose production. Varying doses of flour is expected to affect the expression of AMPK-α2. This study aims to dosage relationship yam flour (Dioscorea esculenta) with tigkat-α2 AMPK enzyme expression in the nucleus skeletal muscle and liver in mouse models of diabetes mellitus. The study was a quasi-experimental design with Post Test Only Group Design. Rats were divided into 5 (five) groups, healthy mice, the mice with type 2 diabetes, and type 2 groups of diabetic rats with dietary intake yam flour addition of 1.25 g (TG-1:25),), 2.5 g (TG -2.5), 5.0 g (TG-5.0). Yam flour is mixed into the rat diet feed with varying doses. The results showed no significant correlation between the dose of yam flour with AMPK-α2 expression levels in skeletal muscle nuclei (p = 0.312) and liver (p = 0.474) in a mouse model of DM. The need for other studies using other types of food as an alternative arrangement of food for patients with diabetes

Analisis Kadar Siklamat pada Es Krim di Kota Banjarbaru Nurlailah Nurlailah; Nurhayati Aslami Alma; Neni Oktiyani
Medical Laboratory Technology Journal Vol. 3 No. 1 (2017): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Sweeteners are one of the components which are often added to foodstuffs. Synthetic sweeteners are widely circulated in the community is cyclamate. Consumption of cyclamate that exceeds the dose will lead to bladder cancer. It will also cause lung, liver, and lymph tumors. The purpose of this study was to determine the presence of cyclamate in ice cream that exceeded the required threshold. The type of this research is the descriptive survey. The sample of this research is ice cream that produced by household companies from all ice cream traders in North Banjarbaru with 11 ice cream sellers. The variables in this study were cyclamate content found in ice cream. The results showed that 11 samples of ice cream were examined, 9 samples containing cyclamate with the highest level of 7.37 g / kg as cyclamic acid. The conclusion of this study is ice cream containing cyclamate found as much as 82% (9 samples), while 18% (2 samples) others negative, from 9 samples containing cyclamate positive, 89% did not qualify the requirements of PERMENKES Number 208 in 1985 that exceeds 2 gr/Kg as cyclamate acid. More specific checks are required for the analysis of cyclamate levels by other methods such as High Performance Liquid Chromatography.

Comparison of Clinical Assessment and Adhesive-Tape Laboratory Microscopic of Sarcoptes scabiei for Scabies Diagnostic Rifqoh Rifqoh; Wahdah Norsiah; Neni Oktiyani
Medical Laboratory Technology Journal Vol. 7 No. 1 (2021): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Scabies infestation caused by Sarcoptes scabiei mite that infected the skin by making the tunnel burrow. Diagnosis of scabies infestation commonly relies on clinical assessment (CA). However, some scabies symptoms are similar to other diseases. Diagnosis of confirmed scabies can be made by identifying egg or Sarcoptes scabiei mite or scybala through skin scraping laboratory microscopic examination, which was the adhesive tape microscopic (AT) a noninvasive alternative. We aimed to compare the scabies diagnostic accuracy of CA and AT in Islamic Boarding School. This study was an analytical observational cross-sectional study. The population was 94 students of Islamic Boarding School, determining the sample base on purposive sampling technique. There were 34 samples with presumptive scabies analyzed with CA by two clinicians and AT by two trained laboratory technicians. The result showed that the number of patients who positive scabies by CA was 14 (41.18 percent) and 31 (91.18 percent) by AT. Sensitivity was 41.93 percent for CA and 92.85 percent for AT, and the difference was significantly based on Cohen's kappa (ⱪ=0.024). The number of positive cases with both techniques was 13 (38.24 percent). The number of patients positive with only CA was 1 (2.93 percent), and only AT was 18 (22.9 percent). The accuracy of CA was only 44,11 percent. The study concluded that CA is low accuracy than AT. The adhesive tape test is easy, needs no expensive equipment. It is recommended that adhesive tape test for screening purposes. The appropriate comprehensive of both methods for scabies diagnostic is highly recommended.

Utilization of Activated Corn Cob (Zea Mays) as an Improved Adsorbent for Reducing Chemical Oxygen Demand (COD) Value from Waste of the Sasirangan Industry Erfan Roebiakto; Noor Hikmah Damayanti; Neni Oktiyani; Nurlailah Nurlailah
Medical Laboratory Technology Journal Vol. 7 No. 1 (2021): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Liquid waste from sasirangan industrial activities has a high enough Chemical Oxygen Demand pollutant power. If it is directly discharged into water bodies, it can damage the environment and harm health. One of the first processes needs to be done by using activated corn cobs (Zea mays). This study aimed to analyze the ability of corn cobs charcoal to reduce levels of Chemical Oxygen Demand and increase the pH of sasirangan waste so that the results of this study can be an alternative to natural-based sasirangan waste treatment. This type of research is a pure experiment with a research design in One Group Pretest Posttest Design. The research material used was sasirangan industrial waste in Manarap Village, Kertak Hanyar District, Banjar Regency, South Kalimantan, Indonesia. Chemical Oxygen Demand levels were determined by the closed reflux titrimetric method. The results showed that the addition of the highest dose of activated corncob charcoal (50 g) reduced the largest turbidity by 35 percent, increased the pH by 72 percent, and reduced the color intensity by 33 percent. The conclusion is that the addition of corncob-activated charcoal at a dose of 30gr, 40gr, 50gr can reduce levels of Chemical Oxygen Demand, respectively, namely 24 percent, 35 percent, and 33 percent. An increase in pH was found at the same dose of 46 percent, 62 percent, and 72 percent, respectively. There is an effect of increasing the mass of activated charcoal from corn cobs on the Chemical Oxygen Demand levels in the sasirangan industrial waste with a significance value of 0.007. It is suggested to use corn cobs-activated charcoal for the pretreatment stage of sasirangan industrial waste treatment.

Evaluation of the Diagnosis of Typhoid Fever Using the Widal Test and the Anti Salmonella typhi IgM Test Wahdah Norsiah; Neni Oktiyani
Medical Laboratory Technology Journal Vol. 6 No. 2 (2020): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Typhoid fever is a systemic infectious disease caused by Salmonella enteric serotype typhi. Laboratory tests are essential because the clinical symptoms of typhoid fever are not specific. The definitive diagnosis of typhoid fever make by culture isolation of Salmonella typhi, but this test is expensive and takes a long time. Other supporting tests that can do quickly are the Widal and Salmonella typhi IgM tests. This study aimed to determine the suitability between the results of the Widal test and the results of the anti-Salmonella typhi IgM test. This type of research is an observational analytic study determining the sample based on the accidental sampling technique. The number of samples taken was 42 samples. The results showed that the Widal Test in typhoid patients was 76% positive and 24% negative. Based on the anti-Salmonella typhi IgM test results in typhoid patients, 38% were positive, and 62% were negative. The study concluded a fragile agreement between the Widal test results and the results of the anti-Salmonella typhi IgM test. It recommended that the Widal test carried out for the initial examination and a quick community survey. The Tubex test highly recommends for diagnosis.

Akurasi Hitung Jumlah Eritrosit Metode Manual dan Metode Otomatis Neni Oktiyani; fahriyan fahriyan; Akhmad Muhlisin
Medical Laboratory Technology Journal Vol. 3 No. 2 (2017): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

In a laboratory, an erythrocyte is done by using hemocytometer and microscope. The task is to measure and assess the size and shape of the erythrocyte. But this procedure is time consuming, complex and tedious. As a solution to this problem, to provide an automated, cost-effective and efficient alternative to detection and counting of erythrocyte, hematology analyzers are used. However, false results related either to erythrocyte or other parameters from complete blood count may be observed in several instances. The objective study was to compare the accuracy of erythrocyte count results of automatic hematological analysis by the manual method. The study is an analytic survey with a cross-sectional design. Erythrocyte counting is done by using three types of control blood, namely high, normal and low, with 9 repetitions. In high control blood, the mean erythrocytes count by the manual method was 7.08 million/μl with a bias value of 1.4%, while that by the automated method was 7.03 million/μl with a bias value of 0.7%. The mean erythrocyte count in normal control blood by the manual method was 4.50 million/μl with a bias value of 0.9%, while that by the automated method was 4.4 million/μl with a bias value of 2.4%. And in low control blood, the mean erythrocyte count by the manual method was 1.72 million/μl with 4.4% bias value, while that the automated method was 1.67 million/μl with 1.2% bias value. Statistical analysis showed no significant difference in accuracy between the erythrocyte count of manual methods with automated methods.

Effects of Dioscorea esculenta and Eubacterium rectale on insulin receptor substrate 1 (Irs1) Expression in skeletal muscle and homeostatic model assessment-insulin resistance (HOMA-IR) in diabetic rats . Sunarti; Tri Setyawati; Neni Oktiyani; Rio Jati Kusuma
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 47, No 3 (2015)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (666.589 KB)

Low expression of insulin receptor substrate 1 (Irs1) is associated with insulin resistance and type 2 diabetes mellitus (type 2 DM). This study was performed to evaluate the effects of Dioscorea esculenta and Eubacterium rectale on the Irs1 expression in the skeletal muscle and the homeostatic model assessment-insulin resistance (HOMA-IR) of diabetic rats. Twenty-five male Wistar rats were divided into five groups i.e. non diabetic rats Group 1; diabetic rats as Group 2; diabetic rats + D. esculenta as Group 3; diabetic rats + E.rectale as Group 4 and diabetic rats + both E. rectale and D. esculenta as Group 5. Rats were made diabetic with induction of intraperitoneally injection of nicotinamide and streptozotocin. After four weeks of the interventions, the blood and skeletal muscles were taken. The Irs1 expression was analyzed with immunohistochemical staining, plasma glucose levels was analyzed using a spectrophotometer, and insulin was analyzed using ELISA methods. All intervention groups reduced plasma glucose levels and HOMA-IRs (p<0.001) and increased Irs1 expression. The greatest reduction of plasma glucose levels and increase of Irs1 expression in the skeletal muscle were found in Group 4, however, the lowest of HOMA-IR was seen in Group 5. These results suggested that D.esculenta, E.rectale, and the combination reduced plasma glucose levels and HOMA-IR by increasing Irs1 expression in skeletal muscle.

Lactic Acid Levels Yogurt Red Beans with Addition of Honey Trigona sp Ruri Widyati; Neni Oktiyani; Haitami Haitami
Tropical Health and Medical Research Vol. 1 No. 1 (2019): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Red beans (Phaseolus vulgaris L.) are grains that are rich in nutrients and useful as a substitute for vegetable milk which can be processed into yogurt using Streptococcus thermophillus and Lactobacillus bulgaricus. Carbohydrates in red beans consist of a group of oligosaccharides that have little ability as an energy source for bacteria to produce lactic acid. Therefore, the making of red bean yogurt needs another source of sugar by adding Trigona sp. Honey. The purpose of this study was to determine the effect of adding Trigona sp honey by 0%, 1.9%, 3.8%, 5.7%, 7.6%, 9.5% to the total level of lactic acid in red bean yogurt incubated for 24 hours at 37oC. This type of research is an experiment with the Postest Only With Control Group Design research design using the alkalimetry titration method. The sampling technique used was purposive sampling technique, namely Trigona sp honey bee taken at Trigona sp bee farm in Tambangan, Tanah Laut. Indonesia. The results showed that there was an effect of adding Trigona sp honey to total lactic acid levels in red bean yogurt with the addition of Trigona sp honey 1.9% - 9.5% fulfilling the requirements of SNI 01.2981-2009. The highest average value of total lactic acid in red bean yogurt with the addition of Trigona sp honey 9.5% which is equal to 0.6672%. It is recommended for future researchers to use other types of carbohydrate sources and additives in the form of proteins. For the community, it is recommended to use alternative sources of other sugar and optimize the addition of Trigona sp. Honey.

Inhibition Strength of Rosella (Hibiscus sabdariffa L.) Boiled Water on Salmonella typhi in vitro Ayu Sutiany; Ratih Dewi Dwiyanti; Neni Oktiyani
Tropical Health and Medical Research Vol. 1 No. 2 (2019): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Rosella (Hibiscus sabdariffa L.) is a plant that can use as herbal medicine. Roselle calyx contains active compounds flavonoids, saponins, and tannins. These compounds can use as an antibacterial against Salmonella typhi. This study aims to determine the Minimum Inhibitory Concentration and Minimum Bactericidal Concentration and the effect of rosella cooking water concentration on Salmonella typhi in vitro. Experimental research with posttest only controls group design through dilution method. The results of the Minimum Inhibitory Concentration (MIC) study showed clarity at concentrations of 60%, 70%, 80%, 90%, and 100%. Minimum Bactericidal Concentration (MBC) results obtained the number of colonies at a concentration of 60% by one colony, at a level of 70%, 80%, 90%, and 100% showed no colony growth. Based on the results of the study concluded that there was an influence of rosella cooking water concentration on the growth of Salmonella typhi in vitro.

The Potential at Phytochemical Compounds from the Indonesian Kalimantan Plant as Covid-19 Immunomodulators Chairana Fadya; Ahmad Luthfi Haqiqi; Annisa Miladdiannur; Monika Yati; Ummu Salamah Az Zahra; Neni Oktiyani; Wahdah Norsiah
Tropical Health and Medical Research Vol. 4 No. 1 (2022): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35916/thmr.v4i1.60

Herbal plants are alternative medicine and immunity boosters related to the Covid-19 pandemic. Typical Kalimantan plants that have the potential are dayak onion (Eleutherine americana Merr.), karamunting (Rhodomyrtus tematosa (Aiton) Hassk), pasak bumi (Eurycoma longifolia Jack), and tigaron (Crataeva adansonii DC). This study aims to determine the potential of phytochemical compounds from Indonesian Kalimantan plants as Covid-19 immunomodulators. Descriptive research with cross-sectional study design by testing the typical plants of Kalimantan, which were extracted first through the maceration method and then carried out qualitative and quantitative phytochemical tests. Qualitative and quantitative results showed that the four positive plants contained phytochemical alkaloids, flavonoids, saponins, and tannins in certain levels. Quantitative results on dayak onion (Eleutherine americana Merr.) obtained alkaloid content; flavonoids; saponins; tannins were 74.540%; 22.125 mg/mL; 58.350%; 0.342 mg/mL. karamunting fruit (Rhodomyrtus tematosa (Aiton) Hassk) 72.815%; 20,500 mg/mL; 78.225%; 0.541 mg/mL. pasak bumi (Eurycoma longifolia Jack) 68.575%; 12,250 mg/mL; 53.930%; 0.383 mg/mL; and tigaron (Crataeva adansonii DC) 82.330%; 40,750 mg/mL; 75.395%; 0.609 mg/mL. The four phytochemical compounds have the potential as Covid-19 immunomodulators. This study concludes that the typical plant from Kalimantan that has been studied has the potential. This research can be continued by testing the effectiveness of phytochemicals as Covid-19 immunomodulators in experimental animals.

Co-Authors Ahmad Luthfi Haqiqi Ahmad Muhlisin Ahmad Muhlisin Ahmad Muhlisin Aima Insana Akhmad Muhlisin AMALIA PUTRI, NADIA Annisa Miladdiannur Anny Thuraidah, Anny Ayu Sutiany Chairana Fadya Erfan Roebiakto Erfan Roebiakto Erfan Roebiakto, Erfan fahriyan fahriyan Haitami Haitami haitami haitami, haitami Jujuk Anton Cahyono Jujuk Anton Cahyono Jujuk Anton Cahyono, Jujuk Anton Kusuma, Rio Jati Mahpolah Mahpolah Meilinda, Intan Monika Yati Noor Hikmah Damayanti Nurhayati Aslami Alma Nurlailah Nurlailah Nurlailah Nurlailah NURUL HIDAYAH Prasetyastuti Prasetyastuti Rahmatus Sholehah Rahmawati Rahmawati Rahmawati Ratih Dewi Dwiyanti Rif'ah, Puteri Amalia Rifqoh Rifqoh Rizky Arsyanti Ruri Widyati Sunarti Sunarti Sunarti, Sunarti Sutiany, Ayu Tini Elyn Herlina Tony Adi Setiawan Tri Setyawati Ummu Salamah Az Zahra Wahdah Norsiah wahdah norsiah Wahdah Norsiah, Wahdah Widyati, Ruri Yanti, Marini Aprida Yayuk Kustiningsih yayuk kustiningsih, yayuk

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