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Jurnal Penelitian Pendidikan IPA (JPPIPA)
Published by Universitas Mataram
ISSN : 24602582     EISSN : 2407795X     DOI : -
Core Subject : Science, Education, Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Materials Science & Nanotechnology Physics
Science Educational Research Journal is international open access, published by Science Master Program of Science Education Graduate Program University of Mataram, contains scientific articles both in the form of research results and literature review that includes science, technology and teaching in the field of science. The Science Educational Research Journal is published twice in a year in January and July editions. The editors receive writing in Indonesian or English, either from the university or from outside the university.
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Articles 31 Documents
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Search results for , issue "Vol. 7 No. 3 (2021): July" : 31 Documents clear
The Use of Tungsten in Potentiometry to Detect Pospat Baffer and Urease Enzyme Victor H Sihombing; Abd Hakim S
Jurnal Penelitian Pendidikan IPA Vol. 7 No. 3 (2021): July
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v7i3.699

Abstract

This study aims to characterize phosphate buffer and urease enzymes through the absorbance spectrum of UV-Vis and FTIR using tungsten as the indicator electrode. The method used in this research is the biosensor potentiometric method carried out in the Laboratory of the Faculty of Mathematics and Natural Sciences, State University of Medan and the Beacukai Belawan Medan laboratory. The absorbance characterization of electrolyte solutions in various compositions using UV-Vis showed that phosphate buffer solution 0.001 M pH 7.5+KCl 0.001 M + urea 0.001 M+3 drops urease enzyme had the highest absorbance compared to electrolyte solutions with phosphate buffer and urea content. Likewise, the FTIR results showed the same thing where phosphate buffer solution was 0.001 M pH 7.5 + KCl 0.001 M + urea 0.001 M + 3 drops urease enzyme had the highest% T (transmission) pattern of phosphate buffer solution and urea. The urease enzyme in this study functions as a catalyst. Based on UV-Vis and FTIR characterization, it was concluded that the phosphate buffer solution of 0.001 M pH 7.5+KCl 0.001 M + urea 0.001 M + 3 drops of urease enzyme was the best.

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