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INDONESIA
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
Published by Pusat Litbang Daya Saing Produk dan Bioteknologi Kelautan dan Perikanan
ISSN : 20895690     EISSN : 24069272     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
Squalen publishes original and innovative research to provide readers with the latest research, knowledge, emerging technologies, postharvest, processing and preservation, food safety and environment, biotechnology and bio-discovery of marine and fisheries. The key focus of the research should be on marine and fishery and the manuscript should include a fundamental discussion of the research findings and their significance. Manuscripts that simply report data without providing a detailed interpretation of the results are unlikely to be accepted for publication in the journal.
Arjuna Subject : -
Articles 8 Documents
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Search results for , issue "Vol 17, No 2 (2022): August 2022" : 8 Documents clear
Preface Squalen Bulletin Vol. 17 No. 2 Tahun 2022 Squalen bulletin
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 17, No 2 (2022): August 2022
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

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Abstract

Characterization of Agarolytic Bacterium Microbulbifer elongatus PORT2 and Its GH16 Agarase Santi Rukminita Anggraeni; Marion B. Ansorge-Schumacher
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 17, No 2 (2022): August 2022
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.653

Abstract

Sugars from agar exhibit potential biological activities and comprise a sustainable source of  third-generation biofuel. Their provision through enzyme-assisted bioconversion of agar is considered a mild and environmentally-friendly process. This work reports a mesophilic-agarolytic bacterium characterization from  Batu Karas, West Java-Indonesia coastal seawater. Analysis 16S rRNA showed a phylogeny relationship of the bacterium to the genus Microbulbifer, especially to M. elongatus. A gene coding for agarase AgaF16A was cloned and expressed in E. coli BL21(DE3) using a genome mining approach. The protein sequence of AgaF16A showed 86.79% similarity to the catalytic domain of glycoside hydrolase (GH) 16 b-agarase from Microbulbifer thermotolerans JAMB-A94. The enzyme cleaved agarose and agar oligomers larger than neoagarooctaose into neoagarohexaose and neoagarotetraose. It also showed activity on natural agar extracted from Indonesian agarophytes. The AgaF16A was active in wide pH and temperature ranges. It retained more than 80% activity after one hour incubation at 50 °C, indicating thermostability, favorable for technical application.
A Review on The Biological, Physical and Chemical Mitigation of Harmful Algal Bloom Nur Hanisah Ibrahim; Anwar Iqbal; Normawaty Mohammad-Noor; Roziawati M.R.; Dede Heri Yuli Yanto; Lee D. Wilson; Abdul Hanif Mahadi
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 17, No 2 (2022): August 2022
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.633

Abstract

The harmful algal bloom (HABs) refers to the rapid growth of toxic or high-biomass-producing microalgae. The impact of this phenomenon can cause significant economic loss affecting many industries and causing harm to wildlife and human health. As technology develops, greater research has been conducted to monitor and reduce HABs occurrence’s impact, including mitigating agents. This review presents the advantages and disadvantages of currently used and recently developed biological, chemical, and physical approaches to tackle issues related to HABs. The present review also emphasizes the interaction between the mitigating agents and the algal cells, thus identifying the gap of knowledge that needs to be addressed. Understanding the advantages and disadvantages of the approaches and the interaction between the mitigating agents and algal cells will enable researchers to develop a better sustainable system for managing HAB.
Anticancer Potential of Three Sea Cucumber Species Extracts on Human Breast Cancer Cell Line Rameshkumar Santhanam; Nurul Shahirah Mohd Azam; Ammira Shafiqha Abdul Khadar; Ambrose Louise; Gregory Dominic; Nur Shahida Ahmad Sofian; See Wee Han; Thiruventhan Karunakaran; Thilahgavani Nagappan; Tengku Sifzizul Tengku Muhammad3; Sevakumaran Vigneswari
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 17, No 2 (2022): August 2022
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.669

Abstract

Sea cucumber has long been utilized as a treatment for a variety of ailments, including antibacterial, antifungal, immunomodulatory, and wound healing. As for the first time, the extracts of three sea cucumber species’ i.e, Actinopyga lecanora, Holothuria atra, and Stichopus vastus, were compared and tested on the cytotoxicity of cancer cells using MTT and Annexin V/Propidium Iodide assays. This study investigates the protection of sea cucumber extracts against the breast cancer cell line (T-47D). All three extracts were found to be actively declining the cancer cell progression, with the IC50 values of 6.25±0.50 µg/ml (A. lecanora), 7.5 ± 1.39 µg/ml (H. atra) and 3.25 ± 0.53 µg/ml (S. vastus). LC-MS/MS analysis was used to identify chemical compunds in the extracts. The 1,1-diphenyl-2-picrylhydrazyl (DPPH), total phenolic and flavonoid contents, and anti-collagenase activity were also assessed in all the three extracts. The results demonstrated the absence of antioxidant and flavonoid chemicals in S. vastus, A. leconara and H. atra extracts. However, H. atra contained phenolic compounds (0.4794 mgGAE/g DW). Furthermore, all tested extracts showed significant anti-collagenase activity, which supported the reduction of cancer cell proliferation. However, more research into the mechanism of action of the extract is needed before sea cucumbers therapeutic characteristics may be used to combat breast cancer.
Front Cover Squalen Bulletin Vol. 17 No. 2 Tahun 2022 Squalen Bulletin
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 17, No 2 (2022): August 2022
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

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Abstract

Prioritization of Food-Chemical Hazard Pairs of Indonesian Fishery Products Based on Export Rejection Year 2017-2019 Nugroho Indrotristanto; Nuri Andarwulan; Dedi Fardiaz; Ratih Dewanti-Hariyadi
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 17, No 2 (2022): August 2022
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.636

Abstract

Necessary approaches are needed to reduce the impact of rejected exported Indonesian seafood due to chemical contamination. This study aims to prioritize food and chemical pairs in the rejected fishery products. The rejection data from three major export destinations: the United States, the European Union, and Japan in 2017-2019 were used. Combinations of food and chemicals were developed and screened, followed by constructing a risk matrix to prioritize the pairs based on health and economic impact. Based on the health impact, a tuna–histamine pair was considered medium risk along with other commodities pairing with heavy metals. Tuna is the most exported seafood and suffers from the most loss; hence it has the highest score for severity and likelihood of economic impact. The combination of health and economic-based prioritization suggested that tuna–histamine was the top priority for immediate mitigation. The second priority consisted of shark–mercury, swordfish–mercury, octopus–cadmium; mahi-mahi–histamine was the third priority. This prioritization can assist risk managers in determining the order of commodities be acted upon based on health and economic considerations to enhance global market access.
Back Cover Squalen Bulletin Vol. 17 No. 2 Tahun 2022 Squalen Bulletin
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 17, No 2 (2022): August 2022
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

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Abstract

Antibacterial Activity of Carotenoid Pigments Produced by Heterotrophic Bacteria from Seawater in Krakal Coastal Area, Yogyakarta, Indonesia Rina Septriani Sidin; Endah Retnaningrum
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 17, No 2 (2022): August 2022
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.648

Abstract

Investigating natural pigments resulting from marine bacterial secondary metabolites is important because of their broad benefits in the food, pharmaceutical, cosmetic, and textile industries. In this study, 52 isolates of pigmented bacteria were isolated from seawater in the Krakal coastal area, Yogyakarta, Indonesia. Seven bacterial isolates showed high pigment stability. Profiling of pigment extracts using a mixture of acetone and methanol with UV–Vis spectrophotometric reading showed that the compounds produced were carotenoids. The highest total carotenoid content with UV–Vis spectrophotometric reading at 480 nm was produced by the Kral-3 isolate (1800 mg/g), followed by Kral-15 (1698.9 mg/g) and Kral-25 (797.2 mg/g) isolates. Analysis of the antibacterial activity of the carotenoid extracts of Kral-3, Kral-15, and Kral-25 isolates using the agar well diffusion method revealed a very strong inhibition score against Escherichia coli. In addition, the carotenoid extract of the Kral-3 isolate showed a strong inhibition score against Staphylococcus aureus, whereas the Kral-15 and Kral-25 isolates showed moderate inhibition scores. Based on the 16S rRNA gene analysis, the Kral-3, Kral-15, and Kral-25 isolates were identified as Kocuria rhizophilla, Calidifontibacter sp., and Rhodococcus ruber, respectively. Based on the findings, the novel bacterial strains are a potential bioresource for the commercial production of natural carotenoids.

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