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(The Effect Of Reconstitution Temperature For Local Isolates Of Enterobacter sakazakii (Cronobacter sp.) from Powdered Infant Formula and Weaning Food) Ramadhani Meutia, Yuliasri; Dewanti-Hariyadi, Ratih; -, Estuningsih
Warta Industri Hasil Pertanian Vol 26, No 01 (2009)
Publisher : Balai Besar Industri Agro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4762.921 KB)

Abstract

Enterobacter sakazakii (recently know as novel genus Cronobacter sp.) is opportunistic bacteria which can cause severe meningitis in neonates. Eight isolates of E.sakazakii which previously isolated from powdered infant formula (PIF) and weaning food were tested foe their ability to survive during reconstitution with water having various temperatures, and their fate during hang time in comparison with 7 isolates previously described by Estuningsih and E. sakazakii ATCC 352/7. reconstitution with 100 C water decreased he number of bacteria of most isolates to undetectable level, while with 40 C and 4 C water did not reduce the bacterial number significantly. Using water of 70 C, reconstitution decreased the number of bacteria of 10 isolates to undectable levels; however 6 isolates survived the reconstitution temperatures. The hang time test showed that some bacteria which were not detected after reconstitution with 70 C water became detectable after 2 hours. Those surviving reconstitution with 70 C well during hang time for 2 to 8 hours.
Antisipasi terhadap Isu-Isu Baru Keamanan Pangan (The Anticipation of New Issues Food safety) Hariyadi, Ratih Dewanti; Hariyadi, Purwiyatno
JURNAL PANGAN Vol 21, No 1 (2012): PANGAN
Publisher : Perum BULOG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (291.288 KB) | DOI: 10.33964/jp.v21i1.97

Abstract

Kemunculan emerging dan kontaminan baru yang berkaitan dengan keamananpangan merupakan isu yang tidak bisa dihindari. E. coli enterohemoragik misalnya,merupakan contoh emerging baru yang menyebabkan kematian dan kerugian ekonomi di Eropa, sedangkan di Cina melamin adalah contoh mutakhir kontaminan pada susu formula bayi yang telah menyebabkan kematian dan kerugian perdagangan di dunia. Paper ini mengulas berbagai potensi emerging dan kontaminan baru yang mungkin muncul dalam sistem pangan. Tujuannya adalah untuk memberikan pemahaman mengenai emerging dan kontaminan baru tersebut sehingga kemunculannya bisa diantisipasi dengan tepat, baik oleh pemerintah emerging pangan maupun konsumen. Penggunaan kerangka kerja analisis risiko merupakan pendekatan logis yang perlu ditempuh oleh pemerintah untuk melahirkan kebijakan dan tindakan keamanan panganyang tepat untuk keperluan antisipasi tersebut. Sementara itu, emerging pangan perlu secara cermat mengkaji ulang praktek dan tindakan keamanan pangan, termasuk program dan rencana HACCP yang ada. Beberapa hal yang perlu dilakukan oleh emerging antara lain, mengevaluasi kembali spesifikasi ingriedien dan titik-titik kendali kritis, mengembangkan program pengambilan contoh dan pemantauan lingkungan, serta menyesuaikan program pengambilan contoh produk. Dalam kaitannya dengan pengendalian kontaminan yang sengaja ditambahkan (intentional contamination), rencana HACCP perlu mencakup juga bahaya-bahaya yang telah diidentifikasi pernah secara sengaja dimasukkan dalam sistem produksi, serta mengidentifikasi dan mengevaluasi bahaya-bahaya lain yang diketahui atau yang diduga bisa secara sengaja dimasukkan sistem produksi.Emerging pathogens and food contaminants important for food safety are inevitable issues. Enterohemorhagic E. coli, for example, is the latest one to cause fatality and economic loss in Europe while in China melamine is a very important adulaterant in powdered infant formula that has cost life and trade worldwide. This paper discusses the emergence of foodborne pathogens and contaminants in food system. The objective is to provide better understanding regarding the emerging foodborne pathogens and contaminants so that their emergence should be well anticipated by government, food industries as well as consumers. Use of risk analysis framework is the logical approach for government to produceappropriate food safety measures and policies. Meanwhile,food industry should take into precaution their existing food safety measures and revisit their HACCP plan. The most likely aspects to be reconsidered include re-evaluation of the specification for ingredients and critical control points, establishment of environmental monitoring and sampling, and adjustment of sampling plan. With respect to preventing intentional contaminations, HACCP plan should include hazards that may be intentionally introduced, identify and evaluate known or reasonably foreseeable hazards that may be associated with the facility. 
PENGARUH PEJANTAN DAN PAKAN TERHADAP GENOTIP PERTUMBUHAN ITIK TURI Dewanti, Ratih
Caraka Tani: Journal of Sustainable Agriculture Vol 25, No 2 (2010): October
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1287.861 KB) | DOI: 10.20961/carakatani.v25i2.14062

Abstract

The experiment was carried out to observe the effect of sires and diets on the growth of genotype traits of Turi duck. There were five sires and 25 dams which produced 151 offspring used in the experiment. Three diets with equal nutrient balance but different concentration P1: {CP:EM= 1:145, EM 2482 kcal/kg:protein 17%}; P2 {CP:EM= 1:146 EM 2628 kcal/kg: protein 18%}; and P3 {CP:EM= 1:144 (EM 2774 kcal/kg: protein 19%} were then applied. Heritability estimation on body weight sire wise was h2: 0.059 (1st week); h2: 0.096 (2nd week), while the estimated heritability for body weigght gain was h2: 0.063 (1st week), h2: 0.239 (2nd week), h2: 0.062 (3rd week), h2: 0.156 (5th week), h2: 0.278 (6th week) and h2: 0.046 (8th week) respectively.
Penghambatan Produksi Aflatoksin dari Aspergillus flavus oleh Kapang dan Khamir yang Diisolasi dari Ragi Tempe Purwijantiningsih, Ekawati; Dewanti-Hariyadi, Ratih; Nurwitri, C. C.; Istiana, Istiana (alm.)
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (192.716 KB) | DOI: 10.24002/biota.v10i3.2874

Abstract

This research was carried out to study the inhibition of aflatoxin production of Aspergillus flavus by moulds and yeasts isolated from ragi tempe. The result showed that moulds found in the ragi tempe consisted of Rhizopus oligosporus, R. oryzae, R. cohnii, Mucor rouxii, Rhizomucor pusillus, Aspergillus niger and miselia sterilia. Yeasts found in the samples were Candida sp1, Candida sp2 and Trichosporon sp. Twelve mould, and yeast isolates were chosen to determine their ability on inhibition of aflatoxin production. Aspergillus flavus alone or in association with each of these isolates were inoculated in Potato Dextrose Broth and analysed for aflatoxin production at day 10. All combinations yielded less aflatoxin than the single culture of A. flavus. All isolates tested could inhibit aflatoxin production. The highest inhibition was shown by Candida sp1 (99,96%).
Penghambatan Ekstrak Etanol Sereh (Cymbopogon citratus (DC) Stapf ) terhadap Produksi Verotoksin Escherichia coli Verotoksigenik Cepeda, Gino Nemesio; Hariyadi, Ratih Dewanti; Supar, Supar
Jurnal Natur Indonesia Vol 13, No 1 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (58.97 KB) | DOI: 10.31258/jnat.13.1.72-76

Abstract

Citronella was a spicy plant used as seasoning in Indonesian food. It was reported to have antimicrobial activity. Itsextract in form of volatile oil prepared by distilation can inhibit the growth of some bacteria. The objective of theresearch was to know the inhibition of ethanolic extract of the citronella on growth and verotoxin production ofverotoxigenic Escherichia coli. The assay of antimicrobial activity was done by using broth dilution and verocellassay. The results indicated that minimum inhibitory concentration of extract can inhibit effectively verotoxinproduction of verotoxigenic Escherichia coli.
PEMBERDAYAAN KELOMPOK PETERNAK ITIK KABUPATEN SRAGEN MELALUI PRODUKSI KONSENTRAT ITIK SECARA MANDIRI SEBAGAI UPAYA SUSTAINABLE USAHA PEMELIHARAAN DAN PENGURANGAN KETERGANTUNGAN TERHADAP PRODUSEN PABRIK PAKAN Dewanti, Ratih; Sudiyono, Sudiyono; Rahayu, Endang Tri
SEMAR (Jurnal Ilmu Pengetahuan, Teknologi, dan Seni bagi Masyarakat) Vol 4, No 2 (2016)
Publisher : LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/semar.v4i2.4542

Abstract

IBM's activities implemented in Nusupan Wetan, Celep, Kedawung, Sragen with partners “Rejeki Agung” Ducks Farmer Group Suhardi and KTI Mandiri. The purpose of activities is to improve the income and welfare of farmers through the ability of farmers in the manufacture of concentrate ducks reducing dependency fromFeedmill.The problems is high price of duck concentrates, has not mastered the preparation of feed,not support equipment manufacture concentrate and lack of knowledge of managerial and bookkeeping. The method used is counseling, mentoring, training, making concentrate, pellet , maintenance practices ducks and mentoring activities. In this activity given mixer machine, pellet machine, oven, concentrate packaging sacks, bamboo cages and feed materials making up the duck concentrate.Results showed dedication that these activities can increase ability and skill of group farmers and productivity of duck concentrate. The day could produce 2000 kg / 10 hours with savings of not buying the concentrate from feedmill Rp.4.460.000/day. Introductions mixer and pellet machines run well. Equipment can be applied properly.Keywords: Animal group Ducks, Concentrate, Sragen
Pemberdayaan Guru Taman Pendidikan Al-Quran Al-Ardhi dan Nurul Jadid di Kabupaten Sragen Melalui Ketrampilan Beternak Ayam Kampung Secara Intensif Dewanti, Ratih; Sudiyono, Sudiyono
SEMAR (Jurnal Ilmu Pengetahuan, Teknologi, dan Seni bagi Masyarakat) Vol 7, No 2 (2018)
Publisher : LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/semar.v7i2.43133

Abstract

Kegiatan PKM dilaksanakan di TPA Al-Ardhi dan Nurul Jadid Kabupaten Sragen. Metode yang digunakan adalah dengan memberikan penyuluhan, pelatihan, dan pendampingan pemeliharaan ayam kampung, penetasan, menyediakan peralatan lengkap, pelatihan pemasaran dan pembukuan sederhana serta pengolahan kotoran. Target luaran yang diharapkan adalah membentuk ustad dan ustadah TPA yang mandiri secara ekonomis dan breeding ayam kampung yang berkelanjutan. Dalam kegiatan ini di introduksikan kandang ren berpelataran, tempat pakan dan minum, mesin tetas full otomatis, eggtray, brooder, ayam kampung 44 ekor dan pakan. Hasil quisioner, terjadi peningkatan hasil sesudah penyuluhan dibandingkan sebelum penyuluhan (pengetahuan meningkat) sesudah penyuluhan yaitu 119 point dibandingkan sebelum penyuluhan 82 point. Puncak produksi telur pada umur ayam 35 minggu sebesar 76%. Penjualan telur perbutir dengan harga Rp.2000-Rp.2500. Penetasan yang dilakukan memiliki daya tetas sebesar 74%. Generasi F1 dijual umur kurang lebih 3 bulan dengan harga Rp.100.000/3 ekor. BEP harga produksi Rp.18.616/ekor. Melalui kegiatan ini, kemampuan dan ketrampilan ustad ustadah TPA Al-Ardhi dan Nurul Jadid mengalami peningkatan dan diharapkan mandiri secara ekonomis.
PEMBERDAYAAN ANAK PANTI ASUHAN AN-NAHL DAN YAUMIHA DI KABUPATEN SRAGEN MELALUI KETRAMPILAN BETERNAK AYAM RAS PETELUR Dewanti, Ratih; Sudiyono, Sudiyono
SEMAR (Jurnal Ilmu Pengetahuan, Teknologi, dan Seni bagi Masyarakat) Vol 6, No 1 (2017)
Publisher : LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/semar.v6i1.20874

Abstract

IbM activity was held at the An-Nahl and YAUMIHA orphanage Sragen. The method were counseling, training, and mentoring maintenance layer, providing complete equipment, marketing and management administration and composting. The target of outcomes are resulting child in orphanage which economically independent, and sustainable of layer egg production. There were 96 bateray cages, place of feed, nipple drinker, eggtray, 96 Brown Leghorn pullet and layer feed given to orphanage. From the results of quisioner counseling increased the value of correct answers (knowledge increased) after counseling was 219 points compared before the 118 point extension. Peak production of eggs at 30 weeks of layer age of 98%. Sales of perkilo eggs with the price of Rp.17.000-Rp.20.000/kg. Business analysis with a net profit of Rp. 23.000,-per day. Through these activities, students' abilities and skills An-Nahl and YAUMIHA orphanage have increased and are expected to be economically self-sufficient. The next plan is for both the partners increase the population of layer by buying pullet in order to expand the business.Keywords: Layer, Farming Skills, Orphanage, An-Nahl and YAUMIHA
SURVIVAL OF Cronobacter sakazakii IN SKIM MILK DURING SPRAY DRYING, STORAGE AND RECONSTITUTION [Ketahanan Hidup Cronobacter sakazakii dalam Susu Skim selama Proses Pengeringan Semprot, Penyimpanan dan Rekonstitusi] Ratih Dewanti Hariyadi; Fenny Larasati; Lilis Nuraida
Jurnal Teknologi dan Industri Pangan Vol. 23 No. 2 (2012): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (403.966 KB) | DOI: 10.6066/jtip.2012.23.2.186

Abstract

Cronobacter sakazakii is an emerging pathogen known to survive dry conditions and its presence in powder infant formula (PIF) has been linked to several outbreaks. In Indonesia, isolation of this bacterium from various foods have been reported. The objective of this study was to determine the effect of spray drying and storage humidity on the survival of C. sakazakii YRc3a in skim milk and their viability upon reconstitution. The survival of Cronobacter during spray drying was determined by comparing the number of bacteria before and after drying. The viability of Cronobacter in spray dried skim milk (SDSM) during storage was observed at weeks 1 to 8 and 12. At the same intervals, SDSM containing the pathogens was reconstituted at either 27°C or 50°C and the survivors were enumerated. The data were plotted to yield survival curves. Spray drying caused 4.19 log CFU/g reduction of Cronobacter and the bacteria experiencing drying were less sensitive to reconstitution at 50°C. During storage, the water activity of SDSM reached equilibrium at week 2 and afterwards, they started to decrease when stored at 50% or 90% RH, but maintained its viability at 70% RH. Storage at 50% and 90% RH accelerated the death rate of C. sakazakii YRc3a, resulting in the decline of the viable counts for 3 log cycles. At 50% RH, C. sakazakii Yrc3a decreased significantly, but the survivors exhibited increased heat resistance with the lowest reduction upon reconstitution at 50°C (0.16 log CFU/ml).
APLIKASI MUTAN BERFLUORESENS UNTUK MEMPELAJARI KETAHANAN HIDUP, KOLONISASI DAN PENETRASI ISOLAT Cronobacter sakazakii SELAMA PENGERINGAN JAGUNG [Use of GFP Mutant to Study the Survival, Colonization and Penetration of Cronobacter sakazakii Isolates Duri Siti Nurjanah; Maggy T. Suhartono; Ratih Dewanti- Hariyadi; Sri Estuningsih
Jurnal Teknologi dan Industri Pangan Vol. 24 No. 2 (2013): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (616.174 KB) | DOI: 10.6066/jtip.2013.24.2.184

Abstract

Cronobacter sakazakii is a Gram-negative emerging pathogen regarded as causative agent of meningitis and necrotizing enterocolitis in certain groups of infants. In the previous research, thirty-two local isolates of C. sakazakii were obtained from various dried food products such as from corn starch, suggesting that they are able to survive drying. Some of the isolates were toxic. Green Fluorescent Protein (GFP) have been inserted to C. sakazakii and used as a marker for selective enumeration due to the ability of this protein to fluoresce under UV and to tolerate in ampicillin containing media. The objective of this study was to evaluate the survival, colonization and penetration of two isolates of C. sakazakii from dried food product during maize drying. The maize was challenged with mutants at a concentration of 105-106 CFU/g before drying. Maize drying was performed at temperature of 40ºC, 45ºC and 50ºC for 4, 6 and 8 days until the moisture content reached 14%. The totals of resistant drying mutants were counted every day onto ampicillin containing media by observing under UV light. The survival rate of C. sakazakii during drying was determined by the slope of linier regression from C. sakazakii survival curve. Isolates of FWHd16, the toxic strain of C. sakazakii, were more resistant to heat treatments in comparison to isolates of YRt2a, or the non toxic strain of C. sakazakii. Following fluorescence and scanning electron microscope observation, it is concluded that both isolates were colonizing on maize surface. These mutants were able to penetrate to the inner side of the grain by entering injured surface or pores at the tip cap of maize.
Co-Authors - Suliantari Abd. Rahman As-syakur Achmad Poernomo Achmad Poernomo Achmad Poernomo Amiroh Amiroh Amiroh Amiroh, Amiroh Arimah Arimah Ayu Intan Sari Aziz, Kholifatul Azura Ulfa, Azura Budi Nurtama C. C. Nurwitri Cahyadi, Muhammad Cynthia Cynthia Cynthia CynthiaCynthia David Yudianto Dedi Fardiaz Ekawati Purwijantiningsih Ekawati Purwijantiningsih, Ekawati Eko Hari Purnomo Endang Tri Rahayu, Endang Tri Ermi Sukasih Estuningsih - Estuningsih - Fadiyah Hanifaturahmah Fenny Larasati Firman Fajar Perdhana Gathot Winarso, Gathot Gino Nemesio Cepeda Harsi D. Kusumaningrum Hesty Nur Fadia Istiana (alm.) Istiana Istiana, Istiana (alm.) Joko Riyanto Karina Nola Sinamo Komala, Kemal Lendrawati Lendrawati Lilis Nuraida Maggy T. Suhartono Maggy Thenawidjaja Suhartono Mala Nurilmala Muhammad Nur Mutaqin, Muhammad Zakki Nandika, Muhammad Rizki Nanin Anggraini, Nanin nFN Setyadjit Ni Gusti Ayu Made Widyatari Asthiti Ni Made Vina Citanirmala Nugroho Indrotristanto Nur Richana Nur Wulandari Nur Wulandari Nuri Andarwulan Nurwitri, C. C. Oryssa Sathalica Pradianti Pawestri, Wari Prasetio, Wiji Pratiwi Yuniarti Martoyo Pratiwi Yuniarti Martoyo Purwiyatno Hariyadi Rahadina Praba Melati Rahmawati Rahmawati Raini Panjaitan Ramadhani Meutia, Yuliasri Ratu Ayu Dewi Sartika Rika Puspitasari MZ Rinto . Sari, Ratna Nurmalita SEDARNAWATI YASNI Shanti Emawati Siti Nurjanah Siti Nurjanah Soegiarto, Salma Rachmanda Sri Estuningsih Suardana, A. A. Md. Ananda Putra Suci Apsari Pebrianti Sudiyono Sudiyono Sukarno Sukarno Supar, Supar Sutrisno Hadi Purnomo Tri Isti Rahayu Ulfah Amalia Uswatun Hasanah Vanessa Len Cahya Agustine Wati, Ari Kusuma Wijaya, Agung Dwi Wilis, Nabila Sekar Winiati P Rahayu Winiati P Rahayu Winiati P. Rahayu Winiati Pudji Rahayu Wiweka, Toh Jaya Yati Maryati Yati Maryati Yesica M. R. Sinaga Yesica Marcelina Romauli Sinaga Yuli Yanti Yuliasri Ramadhani Meutia Yusma Yennie Yusma Yennie