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INDONESIA
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
Published by Pusat Litbang Daya Saing Produk dan Bioteknologi Kelautan dan Perikanan
ISSN : 20895690     EISSN : 24069272     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
Squalen publishes original and innovative research to provide readers with the latest research, knowledge, emerging technologies, postharvest, processing and preservation, food safety and environment, biotechnology and bio-discovery of marine and fisheries. The key focus of the research should be on marine and fishery and the manuscript should include a fundamental discussion of the research findings and their significance. Manuscripts that simply report data without providing a detailed interpretation of the results are unlikely to be accepted for publication in the journal.
Arjuna Subject : -
Articles 363 Documents
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Physicochemical Characteristics of Sodium Alginate Extracted from Turbinaria sp. and Sargassum sp. Rinta Kusumawati; Jamal Basmal; Bagus Sediadi Bandol Utomo
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 2 (2018): August 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i2.297

Abstract

Characterization of sodium alginate extracted from two species of brown seaweeds (Turbinaria sp. and Sargassum sp.) harvested from Binuangeun Beach, Banten, has been conducted. The aim of the study was to evaluate physico-chemical characteristics (moisture, whiteness, viscosity, and functional groups) of sodium alginates extracted from Turbinaria sp. and Sargassum sp. Extraction was conducted in acid solution with the following steps: washing, acid extraction, bleaching, alginic acid conversion, sodium alginate conversion, dehydration, and drying. Each extraction was conducted in duplicate using approximately 1 kg of the dry raw materials (Turbinaria sp. and Sargassum sp.). Results of the analysis showed that the yield of sodium alginate powder extracted from Sargassum sp. was 24.56+0.56% (w/w) with moisture content of 12.69+1.24%; whiteness degree of 43.80+1.71%; and viscosity of 143.43+3.25 cPs, while the yield of sodium alginate powder extracted from Turbinaria sp. was 22.69+2.12% (w/w) with moisture content of 14.77+2.55%; whiteness degree of 23.77+0.68%; and viscosity of 133.67­+4.04 cPs. Meanwhile, the commercial sodium alginate was identified to have moisture content of 16.07+0.09%, whiteness degree of 29.37+0.55% and viscosity of 102.67+4.04 cPs. This indicates that physicochemical characteristics of sodium alginate extracted from Sargassum sp. is better than those extracted from Turbinaria sp. and commercial alginate since it had lower moisture content as well as higher whiteness degree and viscosity.
Potency of Actinomycetes from Deepsea Sediment of Makassar Strait for Producing Antimicrobial Substances Ariani Hatmanti; Puspita Lisdiyanti; Jaka Widada; Subagus Wahyuono
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 2 (2018): August 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i2.351

Abstract

A study on isolation of actinomycetes from sediments of Makassar Strait have been conducted with regard to research project called Widya Nusantara Exploration (EWIN) in May-June 2013 and November 2014. The objectives of this research were to screen antimicrobial activity of 36 actinomycetes from sediments of Makassar Strait, characterized the potential isolates, and determined the metabolites produced by the selected isolate. The antimicrobial screening was conducted using agar diffusion method, while characterization of the best five of actinomycetes were using APIZYM Kit, Scanning Electron Microscope, and FTIR. Five isolates retrieved from this research had ability to inhibit the growth of four microbial testing: Escherichia coli, Bacillus subtilis, Staphyllococcus aureus and Candida albicans. The highest capability was shown by the MACMK-43 isolate that had 16S rRNA gene sequence similarity of 97.85% to Streptomyces violacens. The result shows that, the active fraction contained of 4-amino-5-cyano-6-(4’methoxyphenyl)-1-methyl-2.3-dihydropyrrolo [2,3-B] pyridine, which is commercially used for bactericide and antihistamine.
Preface Squalen Bulletin Vol. 13 No. 2 Tahun 2018 Squalen Bulletin
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 2 (2018): August 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i2.359

Abstract

Probabilistic Health Risk Assessment Due to Natural Formaldehyde Intake Through Opah Fish (Lampris Guttatus) Consumption in Indonesia Ajeng Kurniasari Putri; Umi Anissah; Farida Ariyani; Singgih Wibowo
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 2 (2018): August 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i2.354

Abstract

Opah fish (Lampris guttatus) is one of the bycatch products of Tuna fish catch. The fish currently has become one of the major exported commodities of Indonesia. However, due to its natural deterioration, the fish contained high formaldehyde up to 200 ppm which can be one of health risk when consumed. The aim of this study is to obtain probabilistic health risk assessment data due to consumption of opah fish that contaminated with natural formaldehyde. The study was initiated with analyzing the formaldehyde content of the opah fish (Lampris guttatus) stored in freezing temperature for less than three months. To evaluate the population risk associated with consumption of formaldehyde-contaminated fish, a probabilistic exposure assessment was conducted in several categories based on individual characters who are likely to consume opah fish. Exposures are grouped by sex (male and female) and age (children and adults). To identify the best fit distribution in the probabilistic exposure analysis, fish consumption, body weight and natural occurrence of formaldehyde concentration in Opah fish were fitted in the @Risk®7.0 for Microsoft Excel 2010. Risk exposure analysis was conducted based on the formaldehyde content in Opah fish (real concentration) and two other scenarios of  2 and 4 times of the Opah fish real formaldehyde content. The result showed that Opah fish caught in Indonesian waters and stored in freezing temperature for less than three months, due to deterioration process, could naturally produce formaldehyde ranged from 4,62 ± 0,00 mg/kg to 58,10 ± 0,46 mg/kg. Consequently, based on the probabilistic exposure assessment, female children in Jakarta and Surabaya were considered as in health risk problems. Extremely, the further simulations using two and four times of formaldehyde concentration exposure revealed the health risk  for both gender and all age groups  of Jakarta and Surabaya residents. Therefore, the stakeholders i.e. government and policy makers should take some priorities in formulating a proper risk management strategy on the basis of knowledge of endogenous formaldehyde present in Opah fish as well as risk management strategies for the fish consumer in Indonesia.
Influence of Salinity on Growth and Phycoerythrin Production of Rhodomonas salina Endar Marraskuranto; Tri J Raharjo; Rina S Kasiamdari; Tri R Nuringtyas
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 3 (2018): December 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i3.365

Abstract

Microalgae is a photoautotroph organism capable of producing various photosynthetic pigments with diverse beneficial properties. Rhodomonas salina, a Cryptophyte cell, contains only phycoerythrin as its phycobiliprotein pigment. The effects of salinity on growth and phycoerythrin concentration were investigated. Microalgae R. salina were grown in natural sea water with salinity of 33‰ and 50‰.The microalgae was batch-cultured in f/2 medium at light irradiation of 1100 lux, temperature of 24–26 oC, and photoperiode of 12 h : 12 h. The microalgae cell density was directly calculated using haemacytometer. The concentration of phycoerythrin was determined by spectrophotometric method. The cell density and phycoerythrin concentration were monitored every 4 days for 20 days of cell growth. Results showed that salinity did not affect significantly both on growth and phycoerythrin concentration extracted from R. salina biomass (p0.05; a = 0.05). At both salinity, maximum phycoerythrin concentration were reached on day 8. There was a positive correlation between cell density and phycoerythrin concentration from day 1 to day 8 of cell growth. Microalgae R. salina which was grown in natural seawater with salinity of 33‰ achieved the highest cell density of 8.4 x 105 cells/mL and the phycoerythrin concentration of 0.19 mg. 10-5 cell on day 8 of the culture. The highest phycoerythrin concentration was obtained on day 16 of the culture i.e 0.27 mg. 10-5 cell.Keywords: cell density, growth media, phycoerythrin, Rhodomonas salina, salinity
Method Comparison of DNA Isolation and Quantification for Fish and Seafood Authenticity Determination Dwiyitno Dwiyitno; Stefan Hoffman; Koen Parmentier; Chris Van Keer
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 3 (2018): December 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i3.370

Abstract

Fish and seafood products has been commonly targeted for fraudulent activities. For that reason, authentication of fish and seafood products is important to protect consumers from fraudulent and adulteration practices, as well as to implement traceability regulation. From the viewpoint of food safety, authenticity is beneficial to protect public from serious food poisoning incidents, such as due to ingestion of toxic species. Since DNA based identification depends on the nucleic acid polymerase chain reaction (PCR), the quantity and quality/purity of DNA will contribute significantly to the species authentication. In the present study, different DNA extraction and purification methods (3 classical methods and one commercial kit) were compared to produce the better isolated DNA for PCR amplification. Additionally, different methods for the estimation of DNA concentration and purity which is essential for PCR amplification efficiency were also evaluated. The result showed that classical DNA extraction methods (based on TNES-Urea) yielded a higher amount of DNA (11.30-323.60 ng/g tissue) in comparison to commercial kit/Wizard Promega (5.70-83.45 ng/g tissue). Based on the purity of DNA extract (A260/280), classical DNA extraction method produced relatively similar on DNA quality to the commercial kit (1.79-2.12). Interestingly, all classical methods produced DNA with A260/280 ratio of more than 2.00 on the blue mussel, in contrast with commercial kit. The commercial kit also produced better quality of DNA compared to the classical methods, showing the higher efficiency in PCR amplification. NanoDrop is promising as cheap, robust and safe UV-spectrophotometer method for DNA quantification, as well as the purity evaluation.Keywords: seafood authenticity, DNA isolation, polymerase chain reaction, NanoDrop, Picogreen
Characteristics of Solid Waste Agar Industries Ifah Munifah; Hari Eko Irianto
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 3 (2018): December 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i3.292

Abstract

Agar processed from red seaweed Gracilaria sp. in Indonesia can be found in the form of sheet and powder. The abundance of cellulose in agar solid waste can be used as an alternative source of carbon for microorganism growth. The purpose of this study was to determine the component of agar solid waste and to characterize the cellulose. The agar solid waste (limbah industri agar-agar, LIA) was undergone physical separation process into agar, fiber cellulose, and celite. The result showed that agar solid waste consisted of 53.53% fiber; 37.33% agar and 8.60% celite. LIA was characterized for its components including ash, lignin, extractive substances, cellulose, hemicellulose, and holocellulose using Technical Association of the Pulp and Paper Industry Method (TAPPI). The TAPPI analysis revealed that solid waste generated from seaweed Gracilaria sp processing had 28.19% cellulose, 38.83% holocellulosa, 10.63% hemicellulose, 8.27% ash, 3.54% insoluble acid ash, 11.23% water, and 1.62% extractives substances. The lignin content of the solid waste was low (2.08%), therefore it has potential to be utilized as biomass (bio fertilizer, alternative carbon source). The components in solid waste of agar was determined using Fourier Transform Infra Red (FTIR). The LIA sample had high content of celite indicated by the absorption peak which appears at wave length 2343.45 cm-1 for the -Si-H bond and at the wave length 772.99 cm-1 for the bond -Si- O-. Infra-red spectra showed that celite still exist in solid waste of agar. The study indicated that there was still a large amount of cellulose in the solid waste of agar. Keywords: solid waste, cellulose, agar, lignin, celite
Front Cover Squalen Bulletin Vol. 13 No. 3 Tahun 2018 Squalen Squalen
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 3 (2018): December 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i3.371

Abstract

Metabolite Profiles and Antioxidant Activity of Caulerpa racemosa with Different Handlings sihono, Sihono; Tarman, Kustiariyah; Madduppa, Hawis; Januar, Hedi Indra
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 3 (2018): December 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i3.355

Abstract

 Metabolite profiles and antioxidant activity of Caulerpa racemosa extract with different handlings were investigated. Three different handlings during transportation were applied, namely samples chilled with ice, stored in liquid nitrogen and soaked in seawater. The different handling significantly affected the yield of ethanolic crude extracts and inorganic fractions but insignificantly to organic fractions. Different handlings resulted in differences of major fractions of C. racemosa extracts. Major fractions of the sample that was handled with chilling temperature contained low polar fractions (K10, K11, K12, and K13), while seawater handling extract contained very polar (K1, K2 and K3), polar (K6, K7, and K8) and low polar (K13) fractions. The extract of the sample handled in liquid nitrogen contained balanced fractions. Chilling temperature handling produced highest antioxidant activity (IC50 below 2,000 ppm) in ethanolic extract of C. racemosa. Keywords: antioxidant activity, Caulerpa racemosa, ethanolic extract,handlings, IC50
Back Cover Squalen Bulletin Vol. 13 No. 3 Tahun 2018 Squalen, Squalen
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 3 (2018): December 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i3.373

Abstract

Page 18 of 37 | Total Record : 363

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