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INDONESIA
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
Published by Pusat Litbang Daya Saing Produk dan Bioteknologi Kelautan dan Perikanan
ISSN : 20895690     EISSN : 24069272     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
Squalen publishes original and innovative research to provide readers with the latest research, knowledge, emerging technologies, postharvest, processing and preservation, food safety and environment, biotechnology and bio-discovery of marine and fisheries. The key focus of the research should be on marine and fishery and the manuscript should include a fundamental discussion of the research findings and their significance. Manuscripts that simply report data without providing a detailed interpretation of the results are unlikely to be accepted for publication in the journal.
Arjuna Subject : -
Articles 363 Documents
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PENANGANAN DAN DIVERSIFIKASI PRODUK OLAHAN KERANG HIJAU Murdinah Murdinah
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 4, No 2 (2009): August 2009
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v4i2.149

Abstract

Kerang hijau merupakan salah satu komoditas dari kelompok kekerangan (shellfish) yang sudah dikenal masyarakat, selain kerang darah (Anadara granosa),  kijing Taiwan (Anodonta sp), dan kerang bulu (Anadara inflata). Kerang hijau telah berhasil dibudidayakan dan dapat dipanen setelah 6–7 bulan. Potensi kerang hijau di Indonesia cukup tinggi dan tersebar di beberapa perairan di Indonesia. Kerang hijau mengandung protein sekitar 16,7–21,9%, kaya akan asam amino esensial (arginin, leusin, lisin) dan mengandung mineral kalsium, fosfat, yodium, tembaga. Kerang hijau mengandung daging sekitar 30% dari  bobot keseluruhan dan mempunyai nilai gizi yang tinggi, dengan demikian kerang hijau berpotensi sebagai sumber protein hewani yang relatif murah dibanding ikan. Penanganan terhadap kerang hijau agar aman dikonsumsi dapat dilakukan dengan cara pengurangan kandungan logam berat dengan perendaman dalam larutan kitosan 1,5% selama 3 jam dan teknik depurasi untuk menurunkan kandungan bakteri kerang hijau. Diversifikasi  produk olahan merupakan salah satu cara untuk meningkatkan konsumsi kerang hijau di masyarakat dalam rangka meningkatkan asupan protein dan meningkatkan pendayagunaan hasil perikanan untuk diolah menjadi produk baru sebagai makanan bernilai gizi tinggi, enak, murah, dan mudah didapat. Produk inovatif olahan kerang hijau diantaranya kamaboko, kerupuk,  kerang  rebus  dengan  pewarna  alami, hidrolisat protein, dan pasta condiment. Produk inovatif kerang hijau tersebut mempunyai peluang dikembangkan menjadi komoditi ekspor.
Evaluation of Antibacterial Assays for Screening of Marine Invertebrate Extracts Nurrahmi Dewi Fajarningsih; Ifah Munifah; Dewi Seswita Zilda
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 1 (2018): May 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i1.294

Abstract

Marine environment continuously produces pharmacologically active compounds. To screen marine natural products as a source of potential antibiotics, it is very important to select an accurate and efficient antibacterial assay. This study aims to find out the best assay for screening of antibacterial activity of marine invertebrate extracts by comparing the performance of 3 methods, i.e. the colorimetric resazurin microtiter assay (REMA), disc diffusion assay and spectrophotometric microdilution assay (SMA). Five marine invertebrate extracts, i.e. Stylissa sp., Theonella sp., Lobophytum sp., Sarcophyton sp., and Aaptos sp., were tested using those 3 different methods. The best method obtained was then further tested for its performance to screen 126 marine invertebrate extracts against 2 bacterial strains i.e. Escherichia coli (ATCC®25922TM) and Staphylococcus aureus (ATCC®25923TM). The study showed that, resazurin microtiter assay (REMA) was simpler, produced quicker result and able to generate reliable data for antimicrobial activity screening compared to the other methods. Moreover, REMA assay is regarded as a suitable assay platform to be implemented for screening of marine invertebrate extracts antibacterial activity since it requires only a small amount of extracts. Based on the MIC values, amongst 126 marine invertebrate extracts screened, 59; 36; 25 and 6 extracts tested against E. coli and 69; 36; 18; and 3 extracts tested against S. aureus were respectively categorized as not active, moderately potential, potential and very potential sample worth to be analyzed further. The ability of the REMA to generate accurate MIC value, which is comparable to the existing antibiotic drug MIC value, will empower researchers to decide whether the extracts worth to be examined further or not. 
Endophytic Fungi Isolated from Rhizophora mucronata and Their Antibacterial Activity Kustiariyah Tarman; Dwi Safitri; Iriani Setyaningsih
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 8, No 2 (2013): August 2013
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v8i2.88

Abstract

Mangrove plants are known as source of traditional medicines. Endophytic fungi have an important economic potential for antibacterial, enzyme and secondary metabolites that are useful especially in pharmaceutical industry. The purpose of this research was to determine the antibacterial activity of the endophytic fungal extracts from mangrove plant Rhizophora mucronata against diarrhea causing bacteria. This research included isolation, characterization, cultivation, extraction, and antibacterial activity test. The study yielded 5 types of endophytic fungi with different morphology. Endophytic fungus DS1 was the selected isolate based on antagonism test. The growth pattern showed the stationary phase of isolate DS1 was on the 15th day. Culture broth extract showed considerable inhibition on Pseudomonas aeruginosa with diameter of inhibition zone was 18.5 mm ± 3.32. Extract of mycelium inhibited the growth of Staphylococcus aureus (2 mm). The culture broth extract showed more potent antibacterial activity in comparison with the mycelium extract.
Optimum Age of Starter for Microbial Transglutaminase (MTGase) Production Produced by Streptomyces thioluteus TTA 02 SDS 14 and Characterization of Crude Enzyme Dewi Seswita Zilda; Yusro Nuri Fawzya; Lia Siti Nur'amaliyah; Hana Nurullita Prestisia; Nisa Rachmania Mubarik; Puspita Lisdiyanti
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 1 (2017): May 2017
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v12i1.283

Abstract

The condition of starter plays important role in enzyme production. Optimum starter will lead to the optimum yield of enzyme production. The experiments which were aimed to obtain the optimum  age for starter of Streptomyces thioluteus TTA 02 SDS 14  from solid as well as liquid medium had been carried out which would be used for transglutaminase production. Medium used for culture maintenance, starter optimization and enzyme production was described by Bahrim. The result showed that S. thioluteus TTA 02 SDS 14 was ready to be used as starter after being cultured  on solid medium continued in liquid medium each  for 6 days. The enzyme production in a bioreactor using optimized starter produced enzyme with the highest activity after being fermented for 2 days with 150 rpm agitation. The crude enzyme active optimally at 45-50 oC, pH of 6 and  no effect of metal ion and inhibitor on enzyme activity.  
The potency of finding a new antimalarial drug from Indonesian sea Murtihapsari Murtihapsari; Ekowati Chasanah
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 5, No 3 (2010): December 2010
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v5i3.51

Abstract

Malaria is deadly disease killing 1.5-3 million peoples on the globe a year. In Indonesiaespecially Papua, malaria is a number one killer disease. Finding resources of new drug malariais seriously needed due to resistance to many Chloroquin by Plasmodium. About 65% of marinePorifera from 12 classes in the taxa have recently contributed to our knowledge of biomedicalmaterial. Sponges are abundantely inhabiting Indonesian waters. In recent discovery reportedthat marine sponge Xestospongiafrom Papua has possessed a great ability against Plasmodiumfalciparum.These evidence thus stated Indonesian water is home of the potential marine drug formalaria. For sustainable reason, production live specimen throughout sea culture should to behighlighted. This review presents a development of malaria disease and the potential of Indonesianmarine biota as biomedical material especially for malaria disease.
Influence of Anthropogenic Pressures on the Bioactivity Potential of Sponges and Soft Corals in the Coral Reef Environment Hedi Indra Januar; Ekowati Chasanah; Dianne M. Tapiolas; Cherie A. Motti; Catherine H. Liptrot; Anthony D. Wright
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 10, No 2 (2015): August 2015
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v10i2.108

Abstract

The wealth of marine sponges and soft corals in Indonesian waters represents a rich source of natural products. However, anthropogenic pressures potentially decrease diversity in coral reefs. Presented here are trends for tropical sponge and soft coral biodiversity and their bioactivity potential under the influence of increasing anthropogenic pressures. Samples were collected along transects (near, mid, and far) at Karimunjawa and Seribu Islands Marine National Parks and environmental parameters (salinity, pH, dissolved oxygen (DO), phosphate, nitrate, and ammonia), sponge and soft coral biodiversity, and the bioactivity potential of those organisms (50% Growth Inhibition (GI50) of cancer cell lines H460-Lung, MCF7-Breast, and SF268-CNS) are compared. The environmental conditions and biodiversity were found to be significantly different between groups of sampling sites (P0.05). Canonical Discriminant Analysis (CDA) revealed DO was the discriminant factor driving the separation between groups (90.1%). Diversity tended to be higher in the Far group with strong and significant relation to DO (R= 0.611, P0.05) and ammonia (R = -0.812, P0.05). The CDA also showed that an increase in bioactivity (low % GI50) of sponge and soft coral extracts was related to a canonical function (57.21%) consisting of high DO, high pH, and low nutrients. These findings indicate the production of bioactive compounds is related to diversity and complexity of coral reef systems. Therefore, strategies for marine protection by mitigating the impacts of anthropogenic pressures needs to be optimized in order to conserve the overall environment and sustain its natural bioactivity potential indefinitely.
Risk Profile and Semi Quantitative Risk Probability of Aflatoxin B1 from Aspergillus flavus in a Dried Salted Fish in Several Regions of Java Hidayah, Izhamil; Hermana, Irma; Kusmarwati, Arifah
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 2 (2018): August 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i2.356

Abstract

This study presents a semi-quantitative risk analysis, which determines the probability of aflatoxin B1 exposure from Aspergillus flavus in dried salted fish from the results of research conducted by Indriati about  the prevalence of aflatoxin  B1 in commercial dried fish from some regions of Java. Samples were randomly collected from retailers in Java, such as Banten, DKI Jakarta, West Java, Central Java and East Java, to obtain an approximate level of aflatoxin B1 exposure into Indonesian consumers. The occurrence of the probability of aflatoxin B1 risk from Aspergillus flavus was calculated by statistical, probability approach in @risk version 7.0 software with Monte Carlo simulation. The results of this study showed that the consumption of salted fish was about 3.7 g/capita/day. Hence  there are risks of 7.74 cfu/g A. flavus exposure and 0.7291 ppb aflatoxin B1 exposure in 1 g of a salted fish taken from sampling locations. However this value is still categorized as low risk level.
Front Cover Squalen Bulletin Vol. 13 No. 2 Tahun 2018 Squalen, Bulletin
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 2 (2018): August 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i2.357

Abstract

Back Cover Squalen Bulletin Vol. 13 No. 2 Tahun 2018 Bulletin Squalen
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 2 (2018): August 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i2.358

Abstract

Angiotensin Converting Enzyme (ACE) Inhibitory Activity of Crude and Fractionated Snakehead Fish (Channa striata) Fillet Extract Setyani Budiari; Ekowati Chasanah; Maggy Thenawidjaja Suhartono; Nurheni Sri Palupi
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 2 (2018): August 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i2.345

Abstract

The existence of endogenous bioactive protein or peptide with angiotensin-converting enzyme (ACE) inhibitory activity in snakehead fish fillet is promising to be investigated. The purposes of this research were to extract ACE inhibitory endogenous protein or peptide from snakehead fish fillet and to fractionate the active compounds using ultrafiltration. The extraction employed two solvents, i.e. aquadest and 50% ethanol. Fractionation was conducted using ultrafiltration membranes of 10,000; 5,000 and 3,000 Molecular Weight Cut Off  (MWCO) to separate the protein or peptide into the sizes of 10 kDa, 5-10 kDa, 3 -5 kDa and 3 kDa. The parameters observed were protein and peptide content, ACE inhibitory activity (in vitro) and also protein and peptide profiles. The result revealed that the snakehead fish fillet contained ACE inhibitory endogenous bioactive protein or peptide. The 50% ethanol was more effective in extracting peptide of 10 kDa than the aquadest. Yet, the aquadest was better in extracting higher molecular weight protein of 10 kDa than the 50% ethanol. The fraction of 3 kDa by aquadest had the highest ACE inhibitor activity per g protein (7.85% inhibition of ACE per g protein). Thus, the fraction of 3 kDa aquadest is the most promising option for further research and development of natural anti-hypertension compound. From the result, snakehead fish fillet was potential to be utilized as a functional food as well as functional ingredient to fight hypertension.

Page 17 of 37 | Total Record : 363

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