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Contact Name
Prof. Dr. Ir. Komang G. Wiryawan
Contact Email
kgwiryawan@yahoo.com
Phone
+622518421692
Journal Mail Official
mediapeternakan@apps.ipb.ac.id
Editorial Address
Faculty of Animal Science Building, IPB University (Bogor Agricultural University) Jln Agatis, Kampus IPB Darmaga, Bogor 16680, Indonesia
Location
Kota bogor,
Jawa barat
INDONESIA
Tropical Animal Science Journal
ISSN : 2615787X     EISSN : 2615790X     DOI : -
ropical Animal Science Journal (Trop. Anim. Sci. J.) previously Media Peternakan is a scientific journal covering broad aspects of tropical animal sciences. Started from 2018, the title is changed from Media Peternakan in order to develop and expand the distribution as well as increase the visibility of the journal. The journal is published three times a year in April, August, and December by Faculty of Animal Science, IPB University (Bogor Agricultural University), associated with Animal Scientist's Society of Indonesia. The first edition with the new title will be published in April 2018 edition (Vol 41 No 1 2018), while the previous edition (up to 2017 edition) will still use Media Peternakan as the title and could be accessed in the old website (http://medpet.journal.ipb.ac.id/). This journal has been accredited by National Journal Accreditation (ARJUNA) Managed by Ministry of Research, Technology, and Higher Education, Republic Indonesia with First Grade (Sinta 1) since year 2018 to 2022 according to the decree No. 30/E/KPT/2018.
Arjuna Subject : -
Articles 429 Documents
The Polymorphism and Expression of CYP2E1 Gene and Its Relation to Carcass and Meat Quality of Indonesian Lamb R. S. Harahap; R. R. Noor; A. Gunawan
Tropical Animal Science Journal Vol. 44 No. 4 (2021): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2021.44.4.377

Abstract

The aim of this study was to conduct the genotyping of the single nucleotide polymorphism (SNP) in g.50657948 T>G of the CYP2E1 gene and its relation to the carcass and meat quality. A total of 200 Indonesian lambs consisted of 20 Javanese fat-tail sheep (JFTS), 37 Javanese thin-tail sheep (JTTS), 20 Garut sheep (GS), 21 Jonggol sheep (JS), 34 Garut composite sheep (GCS), 35 compass agrinac sheep (CAS), and 33 Barbados cross sheep (BCS) aged between 10-12 months old were used in this study. The polymorphism of the CYP2E1 gene was characterized using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) with NlaIII enzyme restriction. The CYP2E1 gene found the three genotypes (GG, GT, and TT) and the allele frequencies were in Hardy Weinberg equilibrium. The CYP2E1 gene was significantly associated with meat quality, i.e., pH value and tenderness, as well as fatty acid composition (p<0.05), i.e., saturated fatty acid (SFA): caprylic acid (C8:0), arachidic acid (C20:0), heneicosylic acid (C21:0), behenic acid (C:22:0), and tricosylic acid (C23:0), monounsaturated fatty acid (MUFA): elaidic acid (C18:1n9t) and paullinic acid (C20:1); polyunsaturated fatty acid (PUFA): linoleic acid (C18:2n6c) and y-linolenic acid (C20:3n6). A gene expression analysis indicated that the GG genotype had the highest expression level. It could be concluded that the SNP g.50657948 T>G of the CYP2E1 gene could be used for candidate marker-assisted selection to improve the carcass and meat quality of Indonesian lamb.
Stimulation of IgM Production in HB4C5 Cell Line and Mouse Splenocytes by Egg Yolk Extract from the Egg of Indonesian Native Chicken R. Mulyani; E. Harmayani; Nurliyani; K. Nishi; M. Ishida; T. Sugahara
Tropical Animal Science Journal Vol. 44 No. 4 (2021): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2021.44.4.511

Abstract

Kampung-chicken, as a local wisdom, has always been considered superior in terms of health benefits compared to commercial chicken. Therefore, this study was designed to evaluate the immunostimulatory effects of Kampung-chicken eggs using in vitro approaches. The physical (interior and exterior quality) and chemical characteristics of Kampung-chicken eggs were firstly analyzed; the commercial chicken egg was used as a comparison. The effects of water extract of Kampung-chicken egg yolk (KEYWE) and water extract of commercial-chicken egg yolk (CEYWE) on IgM production and the expression of IgM-gene on HB4C5 cells were analyzed. In addition, the stimulatory effects of both samples on IgA, IgG, and IgM production by mouse primary splenocytes were analyzed as well. To isolate the active substance, the water extract from egg yolk was partially purified by salting out using (NH4)2SO4. Data analysis of the comparison between Kampung-chicken egg and the commercial-chicken egg was performed by t-test; meanwhile, the results for in vitro studies were performed by one-way ANOVA, and Tukey’s multiple comparison test was used for posthoc analysis. The result showed that KEYWE increased IgM production in HB4C5 cells by 5.91 fold as compared with control. In addition, KEYWE increased IgA, IgG, and IgM productions in mouse primary splenocytes by 5.20, 2.54, and 3.1 fold, respectively. Moreover, the immunoglobulin (Ig) production-stimulating activities of KEYWE on HB4C5 cells and mouse primary splenocytes were higher than those of CEYWE. The active substance in Kampung-chicken egg yolk was precipitated at 80% saturation of (NH4)2SO4. Altogether, it is expected that the Kampung-chicken egg has more effective immunostimulatory activity than the commercial chicken egg.
Somatic Cell and Cheesemaking Variables of WAD Goat Milk: Influence of Parity and Lactation Stage A. T. Yusuff; A. A. Badmos; E. V. Awofadeju; A. A. Akintunde; O. I. Alli; V. O. Chimezie; T. R. Fayeye
Tropical Animal Science Journal Vol. 44 No. 4 (2021): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2021.44.4.502

Abstract

The suitability of any goat breed for dairy purposes depends on its assessment of milk-related traits. This study evaluates the influence of parity and lactation stage on somatic cell count (SCC) and cheese-making variables of West African Dwarf (WAD) goat milk. A total of 48 lactating WAD does of three different parities (the 1st, 2nd, and 3rd) were milked over a period of time-phased into three stages of lactation (early, mid, and late). The milk was subjected to SCC and cheese-making during which coagulation time, cheese yield (CY), whey volume, and whey pH were recorded. Collected data were subjected to analysis of variance and Pearsons’ correlation. The result revealed that the milk SCC of the does in the 3rd parity (2.16 x 105 cells) was significantly (p<0.05) higher than 1.89 x 105 and 1.87 x 105 cells in the milk of the 1st and the 2nd parity does respectively. There was a similar variation trend of SCC as lactation progresses in each of the parities. The CY significantly increased (p<0.05) as lactation progresses in the 1st parity (early – 16.95%; mid – 17.21%; late – 20.85%) while, the milk of the 2nd and the 3rd parity from mid to late lactation stage yielded statistically similar cheese that was comparable with late lactation CY of the 1st parity does. The lactation stage and SCC were positively correlated with CY. The volume and pH of the whey were neither affected by the lactation stage nor parity. The study concludes that parity uncovers the effect of lactation stage on the milk SCC of WAD does while the milk obtained from mid to late lactation stage yields more cheese.
Synthetic Gene-Based Heterologous Expression, Proteolytic, and Structural Characterization of Caseinolytic Protease of Lactobacillus plantarum IIA-1A5 M. Yusuf; C. Budiman; I. I. Arief; C. Sumantri
Tropical Animal Science Journal Vol. 44 No. 4 (2021): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2021.44.4.520

Abstract

Genome sequence of Indonesian probiotic of Lactobacillus plantarum II1A5 contains a gene encoding a proteolytic subunit of caseinolytic protease, designated as ClpP_LP. This study aims to express the Clp gene heterological and apply its proteolytic activity to some livestock products. To address this, the gene encoding ClpP_LP was optimized in silico by improving its Codon Adaptation Index and GC content to 0.94 and 53.62%, respectively. The optimized gene was then inserted into pET28a, transformed into Escherichia coli BL21(DE3), and over-expressed by induction of 1 mM Isopropyl β-D-1-thiogalactopyranoside at 37°C. The result showed that ClpP_LP was successfully over-expressed in a fully soluble form with the specific activity towards milk casein was 7739.89 AU mg-1. This activity was significantly greater than that of chymotrypsin. Further, the three-dimensional model of ClpP_LP was built using SWISS MODEL, which showed that this protein formed a homo-tetradecameric (14-mer) structure with each monomer consisting of 7 α-helix and 10 β-sheets. The identification of the active side showed that the active side of ClpP_LP is Ser-97, His-122, Asp-171, and forms a substrate-binding cavity with a size of about 29.5 Ǻ. Overall, our approach can serve as an appropriate platform for the production of ClpP_LP in a large-scale production for various applications in dairy products and derivatives.
Molecular Discrimination between Organic and Conventional Liquid Milk Products in Thailand Using ¹H-NMR Metabolomics Approach T. Phuenpong; M. Kongboonkird; K. Duangmal; W. Lerdvorasap; M. Suksawwawimon; W. Mekboonsonglarp; J. Nuamchit; N. Chantaprasarn; S. Settachaimongkon
Tropical Animal Science Journal Vol. 44 No. 4 (2021): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2021.44.4.478

Abstract

The aims of this study were to characterize and compare non-volatile polar metabolite profiles of organic and conventional liquid milk products using a non-targeted proton nuclear magnetic resonance (1H-NMR) metabolomics approach. Pasteurized plain-liquid milk products from 10 different brands available in Thai marketplace were analyzed for their major chemical compositions and 1H-NMR derived metabolome data. Results demonstrated no specific trend for differentiation between organic and conventional milk samples based on their pH, fat, protein, lactose, and milk solid-not-fat compositions. A total of 45 non-volatile polar metabolites in milk samples were identified by 1H-NMR technique. The chemometric analysis allowed discrimination between organic and conventional milk samples based on their 1H-NMR metabolite profiles. Changes in the relative concentration of formate, betaine, dimethyl sulfone, 2-oxoglutarate, creatine, pyruvate, butyrate, proline, acetoacetate, alanine, glycerophosphocholine, carnitine, and hippurate were statistically identified as potential biomarkers accountable for the discrimination between organic and conventional milk samples in this study. Variations of these compounds might be the reflections of animal diets, rumen fermentation, and physiological adaptation of the cows raised in organic dairy farming systems. Our findings provide new insights and support the effectiveness of using a non-targeted 1H-NMR combined with chemometrics to investigate the molecular authenticity of organic food products.
Tracking of Resistant Salmonella Species in Poultry Farms: New Method of Control Using Essential Oils Nano-Emulsion Conjugated with Antimicrobial Agents H. S. H. Salam; A. N. Mohammed; A. R. Hosni; A. A. E. Shehata
Tropical Animal Science Journal Vol. 44 No. 4 (2021): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2021.44.4.489

Abstract

This work was designed to monitor and track Salmonella spp. in the different internal organs (heart, liver, spleen, and caecum) of 247 bird species (chickens n=176, chicks n=47, ducks n=15, and ducklings n=9) with variable ages in two governorates; El-Fayoum and Beni-Suef, Egypt. Besides assessing the antimicrobial activity of antibacterial agents, essential oils, oils nano-emulsion, and their interactions with each other against salmonellae isolates for their control at the farm level. All samples were collected aseptically for further microbiological and serological investigations. Moreover, the efficiency of essential oils and oils nano-emulsion (thymol, carvacrol, basil, and cinnamon) against recovered Salmonellae were tested using the agar dilution method. A total of fourteen Salmonella serotypes were detected from different investigated internal organs (heart, liver, and spleen), and the three most predominant serovars were S. virchow (17.14%), S. infantis (11.43%), and S. anatum (11.43%). The resistance profile of Salmonella spp. referred to 47.14%, 40.0%, 31.43%, 25.71%, 21.43%, 21.43%, and 21.43% against ampicillin, chloramphenicol, gentamicin, aztreonam, cefazolin, cefotaxime, and tobramycin, respectively. The ability of essential oils (carvacrol oil 0.01%, basil 0.1%, cinnamon 0.01%, and thymol oil 0.01%) to inhibit the growth of Salmonellae differed significantly at 34.29%, 17.14%, 11.43%, and 1.43%, respectively (p<0.05). Oppositely, essential oils nano-emulsion (thymol 0.01%, carvacrol 0.001%, basil 0.1%, and cinnamon 0.01%) showed no inhibitory effect on the growth of Salmonella species. In conclusion, the interactive action between essential oils and antimicrobial agents approved the ability to enhance the susceptibility of the resistant Salmonella isolates against gentamicin, tobramycin, chloramphenicol, and cefazolin. In addition, the interactive action between essential oils nano-emulsion and antimicrobial agents on resistant Salmonella isolates revealed a complete enhanced effect against cefotaxime and variable enhancement against aztreonam.
Ruminal Degradability and Bypass Nutrients of Alkaline or Steam-Treated Cassava Chip and Corn Grain W. Srakaew; C. Wachirapakorn; A. Cherdthong; C. Wongnen
Tropical Animal Science Journal Vol. 44 No. 4 (2021): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2021.44.4.451

Abstract

Modification of starches in high-energy feeds using various treatment methods led to the diverse effects on ruminal degradation and bypass protein and starch. Thus, the objectives of the present experiment were to investigate the effects of modification methods applied to high-starch energy feed sources on ruminal degradability and bypass nutrients determined using in situ and in vitro gas production techniques. This experiment was designed using a 2×4 factorial arrangement in a completely randomized design (CRD). The first factor was high-starch energy feed consisted of two levels, i.e., corn grain and cassava chips. The second factor was the treating method consisted of four levels, i.e., no treatment, steam treatment, NaOH treatment, and steam with NaOH treatment. Chemical composition, ruminal in situ disappearance, bypass of crude protein (CP) and non-fiber carbohydrate (NFC), and gas production characteristics were measured in pre-treated and post-treated samples. The results showed that significant interactions (p<0.01) between high-starch energy feed sources and treating methods were observed with respect to CP, ether extract (EE), and NFC, dry matter degradability (DMD), effective degradation (ED), and effective gas production (EP). Cassava chips had lower CP and EE but higher NFC, DMD, ED, and EP (p<0.01) compared with corn grain. Treating methods have no influence on CP and EE (p>0.05) in cassava chips and corn grain. DMD, ED, and EP of treated cassava chips were lower, while bypass CP and bypass NFC of treated cassava chips were higher than untreated cassava chips (p<0.05). On the contrary, treated corn grain led to higher DMD, ED, and EP, but lower bypass CP and bypass NFC than untreated corn grain did (p<0.01). DMD and gas production characteristics in cassava chips and corn grain showed significant correlations between in situ nylon bags and in vitro gas production techniques within each high-starch energy feed source. In conclusion, steam and NaOH treatments are two alternative methods that can modify the starches of high-starch energy feeds to alter ruminal degradation by decreasing solubility and degradability in cassava chips but increasing degradability in corn grain.
Growth Performance, Blood Profile, and Carcass Characteristics of Weaned Pigs Fed Low Crude Protein Diets Supplemented with Lysine T. K. Ojediran; E. O. Babatunde; S. O. Olokun; O. K. Adigun; B. B. Ajao; F. Emaye; M. D. Shittu; F. A. Adejoro
Tropical Animal Science Journal Vol. 44 No. 4 (2021): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2021.44.4.434

Abstract

Supplementing low crude protein (CP) diets for pigs with exogenous amino acid may help improve growth performance, minimize the environmental impact associated with nitrogen loss and improve the sustainability of pork production. A total number of thirty (30) Large white x Landrace weaned pigs between the age of 8-10 weeks and an average weight of 11.5 ± 0.39 kg were used to evaluate the effect of lysine supplementation of a low CP diet on growth performance, blood profile, and carcass characteristics of weaner piglets. Weaned pigs were acclimatized for seven days, randomly allotted to five (5) dietary treatments with six weaners per treatment and fed a weaner diet based on maize-soybean while wheat bran and dried cassava peel were added to balance the diet nutrients. The animals were allocated to either a control diet containing 22.6% CP or a diet with 2.5%, 5.0%, 7.5%, and 10% reduction in diet CP. Each animal consumed the respective diet for six weeks during which growth performance was monitored. Afterward, 5 mL of blood was sampled for analysis, and all the pigs were slaughtered for carcass analysis. Average daily gain, total weight gain, and average daily intake were not significantly different across the groups (p>0.05). While feed cost per kg was lower with the 7.5% and 10% diet CP reduction (p<0.05), feed cost per weight gain was not different across the animals. Most haematological and serum biochemical variables were not affected by CP reduction, while carcass weight was equally not affected by diet CP (p>0.05). However, the abdominal fat percentage of carcass reduced slightly with the reduced diet CP. Therefore, up to 10% reduction in diet CP from 22.6%, with 0.1% lysine supplementation, had no negative impact on growth without compromising the immunity indicators of weaned pigs. Furthermore, there was no negative consequence on carcass characteristics and the primal cuts.
Clinical Signs and Blood Variables of Pregnancy Toxemia Goats during Late Gestation and Postpartum A. A. Affan; A. Salleh; M. Zamri-Saad; A. Jayanegara; H. A. Hassim
Tropical Animal Science Journal Vol. 45 No. 1 (2022): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2022.45.1.84

Abstract

Pregnancy toxemia is one of the common metabolic diseases in ruminant, which has caused a huge economic impact on the dairy industry. Thus, this study aimed to describe the clinical and blood changes in pregnant goats following induction of pregnancy toxemia. Twelve pregnant goats were divided into control (n=3) and treatment (n=9) groups. The control was fed a diet with adequate energy while the treatment group was exposed to 50% reduction in the energy intake to induce pregnancy toxemia. Blood samples were collected at weekly intervals for biochemical analyses, which included glucose, beta-hydroxybutyrate (BHBA), free fatty acid (FFA), calcium, sodium, potassium, chloride, cortisol, and insulin. On days 20 (PK20) and 40 (PK40) post-induction, 3 induced and 1 control goat was slaughtered while the remaining 3 induced goats were provided with a normal balanced diet to allow recovery (PKRD). The induction resulted in acute pregnancy toxemia after 20 days with clinical signs including weakness, loss of body condition, and recumbency. At 40 days, chronic pregnancy toxemia resulted in signs such as incoordination and abortion. There was significant (p<0.05) decrease in the glucose, insulin, calcium, and potassium levels in the induced goats while the concentrations of BHBA, FFA, and cortisol were significantly (p<0.05) higher. Furthermore, the blood profiling was significantly (p<0.05) different between the PK20, PK40, and PKRD groups and was strongly associated with the presence of clinical signs and ketone bodies in the urine. The 50% reduction in energy intake resulted in acute pregnancy toxemia after 20 days and chronic pregnancy toxemia after 40 days. In conclusion, serum biochemical profile is a potential biomarker to assess the mild and severe pregnancy toxemia in does during the late gestation and postpartum period through changes in blood profiling.
Molecular Detection and Antibiogram of ESBL-Producing and Carbapenem-Resistant Escherichia coli from Rabbit, Swine, and Poultry in Malaysia M. H. Chai; M. Z. Sukiman; N. Jasmy; N. A. Zulkifly; N. A. S. Mhd Yusof; N. M. Mohamad; S. M. Z. Ariffin; M. F. Ghazali
Tropical Animal Science Journal Vol. 45 No. 1 (2022): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2022.45.1.16

Abstract

The emergence of multidrug-resistance Enterobacteriaceae such as extended-spectrum β-lactamase (ESBL) producing Escherichia coli (E. coli) and carbapenem-resistant E. coli (CREC) has become an urgent veterinary and public health threat. These multidrug-resistant microorganisms are frequently associated with diseases that have high mortality with limited treatment options. This study aims to investigate the prevalence of ESBL producing E. coli and CREC from the rabbit, swine, and poultry and to determine the antibiogram profile of these E. coli isolates. In this study, 400 fecal swab samples were collected from rabbits, swine, and poultry from several selected animal farms in Malaysia. After incubation and isolation processes, suspected E. coli isolates were subjected to a PCR test to confirm the identity of the bacteria. The antibiogram of the E. coli isolates was determined via the Kirby Bauer disk diffusion method. A total of 212 (53%) E. coli isolates were isolated from rabbits (51 isolates), poultry (110 isolates), and swine (51 isolates). Screening of antimicrobial resistance genes revealed twelve ESBL producing E. coli (3%; 12/400). Two ESBL producing E. coli were also carrying carbapenemase gene (BlaNDM), indicating ESBL producing and carbapenem-resistant E. coli (ESBL-CREC) in poultry fecal swab samples. The bacteria isolates were found to show resistance against nine antibiotics, including ertapenem, ampicillin, and amoxicillin-clavulanate. A total of 3.3% (7/212) of the E. coli isolates were found to be multidrug-resistance. This study demonstrated the presence of ESBL-producing E. coli and ESBL-producing CREC from poultry fecal swabs in Malaysia.

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