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ANNALES BOGORIENSES

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Published by Lembaga Ilmu Pengetahuan Indonesia

ISSN : 05178452 EISSN : 24077518 DOI : -

Core Subject : Health, Science, Agriculture, Engineering,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology

The Annales Bogorienses (ISSN: 0517-8452, E-ISSN: 2407-7518) is a peer-reviewed Journal that is published biannually. First published in 1955, it is now one of the oldest scientific journal in the nation. The Annales Bogorienses publishes original articles in basic and applied research as well as critical reviews and short communication in the fields of life sciences with the emphasis in biotechnology, molecular biology, and biochemistry.

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Articles 540 Documents

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Growth and Proline Content of Irradiated in Vitro Shoots of Ubi Kuning Cassava Genotype Cultured at Different Temperatures Supatmi Supatmi; Nurhamidar Rahman; N Sri Hartati
ANNALES BOGORIENSES Vol 22, No 1 (2018): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Cassava (Manihot esculenta) is an important crop to food security under climate change due to its various tolerance mechanism under stress conditions. However, the sustainable growth of cassava in the field depends on many factors especially temperature. The objective of the research was to investigate the growth performances and proline contents of irradiated Ubi Kuning at dosage of 10 Gy, cultured in Murashige Skoog (MS) hormone-free solid medium for 4 weeks at three different temperature treatments i.e 25oC, 28oC and 30oC. Each treatment consisted of 3 clone explants with 5 replicates. Results show that growth performances of irradiated plantlets were better compared to that of non-irradiated plantlets in terms of plant height and number of leaves at all temperature tested. The best growth performances were obtained from irradiated plantlets grown under 30oC. The proline content of irradiated Ubi Kuning was high when they were grown under 25 oC and 30oC, implying that these plantlets had the possibility to tolerant to lower and higher-temperature condition. This study is initially useful to find out the growth ability of irradiated Ubi Kuning in response to lower and higher temperature.

Isolation of Endophytic Microbes from Gunung Halimun National Park, West Java, Indonesia and Bioassay Their Potency for Eradicating Microbial Crops Pathogen Endang Sukara; Ruth Melliawati
ANNALES BOGORIENSES Vol 17, No 1 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Gunung Halimun National Park (GHNP) – West Java, Indonesia is the largest preserved primary forest in West Java. Diversity of plants and animals of this park have been studied intensively during the 15 years years. Diversity of endophytic microorganisms, however, has never been reported. Endophytic microbes are those who reside in the interspatial tissues of plants, have a capacity to produce array of secondary metabolites. This paper illustrates the occurrence of endophyte microbes in diverse flowering plants of GHNP. Total of 160 bacteria and 337 fungus were isolated from 86 plants species in the area. Out of that, 159 bacterial isolates and 320 fungus isolates survived in our collection and tested against four major microbial crops pathogen namely Xanthomonas campestris, Pseudomonas solanacearum, Colletotrichum glocosporoides and Fusarium oxysporum cubense. Plate Agar Test Assay method reveals that 51 among 159 bacterial isolates and 62 among 320 endophytic fungal isolates have an ability to inhibit the growth of microbial crop pathogens. Endophytic bacteria can inhibit more microbial crops pathogen but the inhibition ability is less compare to that of endophytic fungi. From our preliminary study clearly shows that endophytic microbes of GHNP should have potential value in developing biological control agent to combat microbial crop pathogens and eventually reduce the use of synthetic chemicals.

Editor's Preface AB Vol 20 No 2 (2016) Muhamad Dzikri
ANNALES BOGORIENSES Vol 20, No 2 (2016): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Rapid Assessment of Diverse Trichodermal Isolates of Indonesian Origin for Cellulase Production Fahrurrozi Fahrurrozi; Shanti Ratnakomala; Trisanti Anindyawati; Puspita Lisdayanti; Endang Sukara
ANNALES BOGORIENSES Vol 14, No 1 (2010): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Trichoderma is a well -known candidate to be promoted as cellulase producer for the hidrolysis of lignocelluloses that contain in woody biomass. The number of trichodermal isolates in our laboratory collected from diverse ecosystem types in Indonesia increases significantly during the last 5 years. It is our aim to assess the cultures for its ability in producing cellulase. Sixty-six trichodermal isolates used in this experiment are obtained from Biotechnology Culture Collection (BTCC), Research Center for Biotechnology, Indonesian Institute of Sciences(LIPI) The 31 isolates were isolated from District of Liwa (South Sumatra, Indonesia) and the 35 isolates from District of Maros (South Sulawesi, Indonesia). The isolates were screened qualitatively, 7 isolates from Liwa and 12 isolates from Maros showed cellulolytic activity. From the results of quantitative test, two strains (ID08-T004 and ID08-T63) showed the higher cellulolytic activity among the selected strains, 133.5 and 133.5 U/ml, respectively. These extracellular enzymes were characterized their temperature and pH optimum. The temperature optimum for both enzymes was the same, 50C, with activity 213.6 U/ml for enzyme extracted from ID08-T004 and 197.3 U/ml for enzyme from ID08-T0063. The pH optimum was pH 5 of ID08-T004 with activity137.7 U/ml and pH 6 for ID08-T063 with activity 75.0 U/ml. The enzymes from ID08-T004 and ID08-T063 were stable in their temperature and pH optimal condition even after 90 minutes incubation with activity 179.0 U/ml and 86.7 U/ml, respectively. The enzyme stability was approximately 150 minutes for both enzymes in the temperature and pH optimum. Key Words: Trichoderma, cellulase, Culture Collection

In Vitro Induction Of Tetraploid Pummelo ’Nambangan’ (Citrus maxima (Burm.) Merr.) By Colchicine Treatment Using Germinated Seed, Shoot Tip And Cotyledonary Node As Explants Dyah Retno Wulandari; Agus Purwito; Slamet Susanto; Ali Husni; Tri Muji Ermayanti
ANNALES BOGORIENSES Vol 19, No 1 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Tetraploid citrus are important for interploidal hybridization to create triploid seedless citrus. Colchicine is the most commonly used as antimitotic agent to induce polyploid plants. Tetraploid induction by colchicine in Pummelo ‘Nambangan’ was conducted in vitro using different types of explants. The aim of this research was to induce tetraploid pummelo ‘Nambangan’ by colchicine treatment using germinated seed, shoot tip and cotyledonary node as explants. Tetraploid shoot induction was conducted by soaking germinated seeds, shoot tips and cotyledonary nodes in 0.1% colchicine for 1, 3 and 5 h. Regenerant shoots were grown on MS medium and their growth was observed after four weeks in culture. Ploidy level was determined using flow cytometry analysis. Stomata density, length and width of stomatal guard cell were also recorded. The results showed that shoot elongation was inhibited by colchicine treatment. Soaking of shoot tip explants in 0.1% colchicine for 1 h resulted in 66.66% of putative tetraploid shoots. Compared to diploid shoots, tetraploids had lower stomata density but bigger in guard cell size. Keywords: colchicine, tetraploid, pummelo (Citrus maxima (Burm.) Merr.), flow cytometry, stomata,

Editorial Boards AB Vol 15 No 2 (2011) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 15, No 2 (2011): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Laccase and Manganese Peroxidase (MnP) Activities in the White-Rot Fungus Trametes hirsuta in Response to Aromatic Compounds Isa Nuryana; Zidny Ilmiah; Ade Andriani; Yopi Yopi
ANNALES BOGORIENSES Vol 23, No 2 (2019): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

The current interest in exploring white-rot fungi has been concentrated in increasing their ligninolityc enzyme production such as laccase and manganese peroxidase (MnP) due to the great value in industrial application. The presence of appropiate inducers can enhance the enzyme production. Hence, the aim of the study was to investigate the activity of laccase and MnP in response to various aromatic compounds. The fungus Trametes hirsuta was cultured in Glucose Yeast Peptone (GYP) broth (pH 5.0) with the addition of 0.5% of sorghum biomass as substrate. The cultures were then incubated on rotary shaker at 150 rpm at 27oC. The 7-day-old cultures were then supplemented with different aromatic compounds, namely caffeic acid, gallic acid, syringic acid, trans-cinnamic acid, vanillin, and veratryl alcohol. Our results demonstrated that veratryl alcohol, gallic acid and vanillin gave an inductive effect on the value of laccase and MnP activities, with vanillin showing the highest induction. On the contrary, the level of laccase and MnP activities remained low in the presence of aromatic compound such as syringic acid, trans-cinnamic acid and caffeic acid. To conclude, our study reveals that aromatic compounds can be potential as inducers and may contribute to the improvement of laccase and MnP productivity by T. hirsuta.

A Highly Abundant Lectin Protein in Arabidopsis thaliana Confers Resistance Against Pathogens Maria prihtamala Omega; Skye Thomas Hall; Peer Schenk; Bostjan Kobe
ANNALES BOGORIENSES Vol 12, No 1 (2008): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Lectins are glycoproteins that recognize and bind to specific carbohydrates. they are involved in a range of biological functions, such as plant defence, storage proteins seed germination and plant microbe interactions. Lectin 3.1 (At3g 15356) is a protein in plant model , Arabidopsis thaliana, that has been shown to be up-regulated in all defence pathways, especially in responce to methyl ester jasmonate (MJ) . All thal was known about the gene was that it had good homology to the beta domain of legume lectins. That aim of this project was to characterize the structure and function of the Iectin protein using CD spectra and X-ray crystallography. A TDNA insertion line for the lectin gene and a number of 35S over-expression lines that had varying levels of expression had been generated, but none of these showed any obvious phenotype. Two protein bands were observed on Comassie stained SDS-PAGE gels in the over-expression lines and in MJ induced wild-type (WT) . The two protein bands represented two isoforms of the lectin 3.1 protein ; in a glycsylation assay the larger protein band was shown to be heavily glycosylated . A nematode (M. incognita) disease assay djscovered that the lectin over-expression lines had less nematode eggs compared to that of the WT and that the insertion line had more nematode eggs than the WT. This data provides evidence that lectin 3. 1 improves. plant resistance against M. incognita infection. interestingly, the nematode gut lining contains fucose with which Iectin 3.1 binds to. Keywords: Arabidopsis thaliana, lectin’s structure and function, pathogen resistance

Improvement of Endoglucanase Activity in Penicillium oxalicum ID10-T065 Mutated by Ultra Violet Irradiation and Ethidium Bromide Asnany Caniago; Wibowo Mangunwardoyo; Sukma Nuswantara; Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 19, No 2 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Penicillium sp. is known as filamentous fungi that produce complete cellulase. Cellulase. This study aims to improve endoglucanase activity of Penicillium oxalicum ID010-T065 by mutated with ultra violet irradiation (with dose of 0.1 J/cm2, 15 cm), ethidium bromide (10 µg/mL, 1 hour) and combination of both mutagens. The endoglucanase activity of all mutants was higher than that of the wild type (1.03 U/mL). Mutant UVEB-42 exposed to combine mutation showed the highest endoglucanase activity (2.76 U/mL) with a 2.70 fold increase. Mutant EB-45 (1.83 U/mL) exposed to ethidium bromide solution showed a 1.8 fold increase. Mutant UV-13 (1.72 U/mL) exposed to UV irradiation for 3 minutes showed a 1.7 fold increase. All mutants have optimum endoglucanase activity at 50 °C. Mutant UVEB-53 showed the highest thermostability by retaining 86 % of endoglucanase activity at 90 °C. The gene analysis of the endoglucanase I gene showed 3 bases mutated at mutant UV-13 and UVEB-53 that changed proline to serine. Mutant EB-45 showed 4 bases mutated that changed valine to glysine and proline to serine. Two bases mutated at Mutant UVEB-53 changed proline to serine. Bases mutated in eg1 gene could influenced the enhance of enzym activity in mutant.

Application of Microwave Heating in Biomass Hydrolysis and Pretreatment for Ethanol Production Euis Hermiati; Djumali Mangunwidjaja; Ttiti C Sunarti; Ono Suparno; Bambang Prasetya
ANNALES BOGORIENSES Vol 14, No 1 (2010): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Recently, due to depletion of fossil derived energy stock in the world, there are growing interests in utilizing biomass sources of bioethanol. There are basically two types of biomass that are usually used or converted to ethanol, starchy and lignocellulosic biomass. The conversion of starchy and lignocellulosic materials are widely explored, however, there are still some drawbacks, such as high enzyme cost and intensive energy needed. Therefore, lower cost as well as energy and time efficient process technology in biomass conversion to ethanol is important in enhancing the use of biomass to substitute fossil fuel. Microwave heating offers some advantages to overcome these drawbacks, especially due to its quick heat transfer and its heating selectivity. In conventional heating the heat was transferred through conduction or convection process which took longer time. Thus, by using microwave, degradation of starchy and lignocellulosic biomass could be completed in shorter time than by using conventional heating method. The roles of microwave heating in the degradation of biomass, especially starchy and lignocellulosic biomass and its relation to the hydrolysis and pretreatment of that particular biomass for ethanol production are reviewed and discussed. Keywords: biomass, pretreatment, ethanol, microwave

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