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INDONESIA
Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia)
Published by Perkumpulan Obstetri dan Ginekologi Indonesia
ISSN : 23386401     EISSN : 23387335     DOI : -
Core Subject : Health,
Health Professions Medicine & Pharmacology Public Health
The Indonesian Journal of Obstetrics and Gynecology is an official publication of the Indonesian Society of Obstetrics and Gynekology. INAJOG is published quarterly.
Arjuna Subject : -
Articles 8 Documents
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Search results for , issue " Volume. 34, No. 1, January 2010" : 8 Documents clear
High Calcium and Vitamin D Fortified in Enhancing Postmenapausal Bone Turnover Arifin, Zainal
Indonesian Journal of Obstetrics and Gynecology Volume. 34, No. 1, January 2010
Publisher : Indonesian Socety of Obstetrics and Gynecology

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Abstract

Objective: To compare the effect of consuming vitamin D fortified high calcium milk (HCM) with placebo milk supplementation’s effect to bone formation and resorption’s marker to post-menopausal women in Jakarta, Indonesia, in four months duration. Methods: This is a double blind randomized control trial to know the effect of high calcium (1200 mg/day) milk consumption compared to low calcium milk consumption to the change of bone turnover’s chemical marker in post-menopausal women in Indonesia. Participants are women aged more than 55 years, which are patients of Yasmin Menopause and Osteoporosis Clinic, RSCM. Subject of study is classified into two groups, the first group was given two portion (60 g) of high calcium milk (HCM), and other group as control was given two portion (60 g) of low calcium milk, each day for 16 weeks. A comparison between the level of bone markers (CTelopeptide (CTX), Peptide Procollagen-I (P1NP), Osteocalcin (OC)) in week 0, 2, 8 and 16 was done. Post intervention, the result was statistically calculated with ANOVA and the mean result comparison to subject was analyzed with Tukey-Kramer test. Results: There are no significant differences between two groups on age, Body Mass Index (BMI), Bone Mineral Density (BMD) and calcium intake characteristics. There is CTX (CTelopeptide) reduce to 34% in two weeks of supplementation to HCM group cohort. OC (Osteocalcin) was also reduced in 16 weeks supplementation to subject group, reduced to 10% on week 2, 18% on week 8, and 32% on week 16. Peptide Procollagen-I (P1NP) was reduced to 15% on week 8, and 28% on week 16 in subject group. High calcium and vitamin D fortified milk that was used, is proven could improve vitamin D state, reduce PTH level and reduce bone turnover significantly. Conclusion: High calcium and vitamin D fortified milk supplementation gives better result compared to low calcium milk supplementation in reducing bone turnover in post-menopausal women. [Indones J Obstet Gynecol 2010; 34-1:31-8] Keywords: post-menopausal women, high calcium and vitamin D fortified milk, C-Telopeptide, Peptide Procollagen-I, Osteocalcin, vitamin D state
Distribution of Regulatory T-Cell (Cd4+, Cd25+) in the Peritoneal Fluid of Endometriosis Patients Rusdi, Gazali
Indonesian Journal of Obstetrics and Gynecology Volume. 34, No. 1, January 2010
Publisher : Indonesian Socety of Obstetrics and Gynecology

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Abstract

Objectives: To observe the distribution of Regulatory T-cell (CD4+, CD25+) in the peritoneal fluid of endometriosis patients, as related to the local immune system of the peritoneum. Method: A cross-sectional descriptive research Perioneal fluid was taken from endometriosis patients and non-endometriosis patients who underwent laparoscopic or laparotomy surgery at Raden Saleh Reproductive Health Clinic and Central Operating Theatre between June 2008 to March 2009. Total leukocyte and its differential count was counted manually. Regulatory T-cell was labelled with monoclonal antibodies for CD45 (PerCP-347464), CD4 (SIPC- 340133) dan CD25 (PE-341009) and counted by Flowcytometry (Facscalibur) using cellquest pro software. Data were collected and analyzed with SPSS ver 11 software. Result: Thirty-three patients were participated in this study, 12 non-endometriosis and 21 endometriosis patients were analyzed. 10 endometriosis patients were classified as minimal-mild, while 11 others were in moderate-severe stage. Higher median level of Regulatory T-cells were found in endometriosis patients (0.52%) compared to non-endometriosis cases (0.12%), even it was not significantly different (p 0.403). The median level of Regulatory T-cell in minimal-mild endometriosis (0.46%) was not significantly different with moderate severe endometriosis (0.52%) (p 0.981). Conclusion: The percentage and total number of Regulatory Tcell in the peritoneal fluid did not show significant difference between endometriosis and non-endometriosis patients. Endometriosis patients shows tendency of higher percentage and total number of Regulatory T-cell than non-endometriosis group. The stage of endometriosis apparently does not show any significant difference in terms of Regulatory T-cell in peritoneal fluid. [Indones J Obstet Gynecol 2010; 34-1:19-23] Keywords: Regulatory T-cells, CD4 CD 25, peritoneal fluid, endometriosis
Not only embryo quality but also Endometrial Thickness Contributes to IVF outcome: a retrospective study of all IVF cycles in Yasmin Clinic, Jakarta, Indonesia Wiweko, Budi
Indonesian Journal of Obstetrics and Gynecology Volume. 34, No. 1, January 2010
Publisher : Indonesian Socety of Obstetrics and Gynecology

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Abstract

Objective: To study the endometrial thickness and embryo quality during In Vitro Fertilization (IVF) cycles in predicting IVF outcome. Methods: This retrospective study involved 206 infertile patients undergoing 218 IVF cycles in Yasmin Clinic, Dr. Cipto Mangunkusumo National Referral Hospital, Jakarta, Indonesia. All IVF cycles were performed from January 2005 until May 2009. The outcome of this study is the clinical pregnancy following IVF cycles. The endometrial thickness was measured on the day of human chorionic gonadotrophin (hCG) administration. The number of embryos that developed ≥ 8 cells on cleavage II represented as the embryo quality. Results: There were 51 among 218 cycles (23.4%) resulted in pregnancy. The endometrial thickness on day of hCG administration was significantly higher in pregnant group compared to non-pregnant group (11.49±1.97 mm versus 10.13±1.93mm; p
Ovarian reserve Loho, Maria Flavia
Indonesian Journal of Obstetrics and Gynecology Volume. 34, No. 1, January 2010
Publisher : Indonesian Socety of Obstetrics and Gynecology

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Abstract

Objective: To asses ovarian reserve (OR) is important in infertility management that will undergo Assisted Reproductive Technology (ART) programs. using some methods, eg. physical measurements: ovarian volume, Antral follicle Count (AFC); Biomarker assay, indirect and direct methods, Day 3 FSH, Anti-Mullerian Hormone (AMH), Inhibin B. Method: Literature review in published studies which assessment the Ovarian Reserve. Conclusions: Assessment of ovarian reserve (OR) is very important to predict the outcome of ART programs. There were several methods to assessing the OR, but none as a single predictor. The best candidate for a single biomarker is AMH. [Indones J Obstet Gynecol 2010; 34-1:43-6] Keywords: predictor of ovarium reserve, assisted reproductive technology
Increment of placental Caspase 3 expression in preeclampsia and its effect on birth weight and blood pressure Teguh, Mintareja
Indonesian Journal of Obstetrics and Gynecology Volume. 34, No. 1, January 2010
Publisher : Indonesian Socety of Obstetrics and Gynecology

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Abstract

Objective: To determine the placental apoptosis process in preeclampsia. Methods: This study was an observational analytical study using cross sectional design. This study was performed at Dr. Hasan Sadikin Hospital, during April to June 2008. Immunohistochemical analysis was used to examine the placental expression of kaspase 3. Results: Placental expression kaspase 3 was significantly higher in preeclampsia group (21,9%) than in the normal pregnancies (0) with p=0,006. There was significantly correlation between placental kaspase 3 with birth weight in preeclampsia on the otherhand, there was no significant difference of placental expression kaspase 3 with birth weight in normal pregnancies. There was no significant difference of placental expression kaspase 3 with mother’s blood pressure in the two group. Conclusion: there is a significant increase of placental expression of kaspase 3 in preeclampsias. [Indones J Obstet Gynecol 2010; 34-1:3-6] Keywords: caspase 3, preeclampsia, apoptosis.
Early Experience of Laparoscopic Radical Hysterectomy and Lymphadenectomy Aziz, Mohammad Farid
Indonesian Journal of Obstetrics and Gynecology Volume. 34, No. 1, January 2010
Publisher : Indonesian Socety of Obstetrics and Gynecology

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Abstract

I have had a basic laparoscopy skill during my residency in the Department of Obstetrics and Gynecology University of Indonesia back in 1974. At that time this procedure only for diagnostic purpose especially to determine the patency of the fallopian tube. Time goes on and laparoscopy now becomes very popular surgical procedure as a minimal invasive surgery in almost all of the surgical procedure not only in gynecologic field but also has expanded to digestive surgery, orthopedic, ENT, thorax surgery. Prof. Joo-Hyun Nam, MD (Prof. Nam) from Korea suggested me to develop this kind of surgery. Gynecologic oncologist should have competency for doing this minimal invasive surgery such radical surgery in cervical cancer, endometrium and early ovarian cancer or just for surgical staging includes paraaortic lymphadenectomy and omentectomy. Before I involved deeply in laparascopic gynecologic oncology surgery, I have learned much form dr. Wachyu Hadisaputra, as the chairman of the gynecologic endoscopy working group from POGI (Indonesian Society of Obstetrician and Gynecology), in laparoscopic gynecologic non oncology surgery. In late 1990 I attended a symposium Laparoscopy in Gynecologic Oncology in Philadelphia organized by the late Prof. Dargent under IGCS (International Gynecology Cancer Society). In 2005 I followed a workshop for radical hysterectomy and lymphadenectomy on unbalmed cadaver (fresh cadaver) in Florida during Annual Meeting Society of Gynecologic Oncology, and I joined for the next year workshop. The trainer came from prominent countries such as USA, Germany and France. Since then I practiced total hysterectomy with laparoscopic surgery, even in small number of cases. In early 2009 I had an opportunity to visit Prof. Nam hospital in Seoul and watched him doing live radical surgery in the operating theatre. In the same year I practiced paraaortic lymphadenectomy on swine in Shanghai. Back from Seoul I and dr Chamim started doing radical surgery at Fatmawati Hospital and months later I followed an unbalmed cadaver Laparoscopic Symposium in Oncology in Taichung Taiwan. Another case done at Omni Hospital to fullfil dr. Boy Busmar’s invitation. From what I had been experienced I can suggest that we have set a solid team which is very important and supported by good equipment such colpotomy device, bipolar dissection, scissor, hormonic and ligaclip are very helpful if possible but if is available enough with bipolar dissection. The first step to assess the internal genital, if there is a massive adhesion it would prolong the surgery time. We then opened or incised the peritoneum between round ligamentum and fimbria and extended medially and laterally to exposed psoas muscle and ureter which cross the common iliac artery. The round ligament should stay intact to ease the surgery and prevent the uterus not to distort. Vesico-uterine fold was opened and we made a space such as paravesical and pararectal spaces. Then the procedure was done medially to extract fat and node ventral to common iliac until the wall of the artery was noted and we did lymphadenectomy along the external iliac artery. By doing this procedure, the iliac vein, internal iliac and uterine artery will be exposed and obturator nerve as well. The nodes was then put in the plastic bag made of plastic drug so it is very cheap instead of special bag sold by the supplier. Ureter was disected and pushed aside and ureteric canal was opened. Vagina was amputated by direction of colpotomy device and top of the vagina sutured through the vagina as suggested by Prof. Nam. By doing this if we think that vagina cut was inadequate, we can cut it more. The beginning of the procedure took more than 4 hours and as mentioned by the literatures that the learning curve will decrease by the amount of surgery. We have done 5 cases, 1 of those with serosal laceration of the sigmoid and repaired the lacerated serosal with few stitches and 1 case with iliac vein and the bleeding could be controlled using ligaclip. I hope that this minimal invasive surgery will enrich our modality in handling the malignancy in gynecologic surgery and we can positioned to the level of developed countries in Asia. The Asian Society of Gynecologic Oncology has planned to train Young Gynecologic Oncologist in this kind of surgery. In July 2010 there will be a workshop laparoscopy in gynecologic Oncology in Seoul and Indonesian Society of Gynecologic Oncology (Himpunan Onkologi Ginekologi Indonesia) is asked to propose candidates. Accommodation and transportation while in Korea will be covered by ASGO. I hope, this invitation can be responded well by our young gynecologic oncologist.
Comparison of Estrogen β Receptor Expression and the Addition of Various Dose of Geistein to HUVEC Endothel Cell which Exposed to Oxidative Stress Handayani, Vera
Indonesian Journal of Obstetrics and Gynecology Volume. 34, No. 1, January 2010
Publisher : Indonesian Socety of Obstetrics and Gynecology

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Abstract

Objective: To compare β estrogen receptor on HUVEC endothelial for each Genistein dose addition with β-estradiol physiologic dose. Methode: Experimental study performed in Physiology Laboratorium, Medical Faculty of Brawijaya University Malang, HUVEC culture exposed by glucose as a dysfunction endothelial model. Endothel collected from female newborn umbilicus was cultured to make a confluent culture. The culture was divided into 7 groups: 1. HUVEC without treatment, 2. HUVEC + glucose 33 mM, 3. HUVEC + glucose 33 mM + 17β estradiol , 4. HUVEC + glucose 33 mM+ Genistein 2.5 μm. 5. HUVEC + Glucose 33 mM + Genistein 5 μm, 6. HUVEC + Glucose 33 mM + Genistein 7,5 μm, 7. HUVEC + Glucose 33 mM + Genistein 10 μm. Culture then was incubated in 37°C and stained with immunohistochemistry, using primary antibody of β estrogen receptor. The expression was observed on the -15, 30, 60, 120 and 240th minute. The datas from observation result was analysed with ANOVA test and correlation test. Result: Estrogen receptor comparison between group 1 was significantly different with group 2, 3, 6, 7 and not significantly different with groups 4, 5. Estrogen receptor between group 2 was significantly different with group 4, 5 but not significantly different with groups 3, 6, 7. Estrogen receptor comparison between group 3 was significantly different with group 4, 5 and not significantly different with groups 6, 7. Estrogen receptor comparison between group 4 was significantly different with group 6, 7 and not significantly different with groups 5. Estrogen receptor comparison between group 5 was significantly different with group 6, 7. Estrogen receptor comparison between group 6 was not significantly different with group 7. Isoflavon Genistein dose increase on 30, 60 dan 120 minutes will reduce β estrogen receptor expression on endothelial HUVEC and on 15 and 240 minutes shows that there was no significant correlation with β estrogen receptor expression on endothel HUVEC. Conclusion: There was significant expression distinction of ERβ in glucose induced HUVEC exposed by 17β estradiol compared with those exposed by Genistein but not in Genistein dose 7.5 and 10 μm. The lower of Isoflavon Genistein dose in the -30, 60 dan 120 minute will cause expression of β estrogen receptor in HUVEC endothelial cell that tends to be higher. [Indones J Obstet Gynecol 2010; 34-1:24-30] Keywords: β estrogen receptor, HUVEC, genistein, estradiol.
The use of elastic stocking to reduce the risk of varicose vein Sastrawinata, Ucke Sugeng
Indonesian Journal of Obstetrics and Gynecology Volume. 34, No. 1, January 2010
Publisher : Indonesian Socety of Obstetrics and Gynecology

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Abstract

Objective: To reduce the risk of varicoses in primigravida by wearing elastic stockings with mechanical compression. Method: A prospective study was performed in a randomized single blinded trial with analysis of variance and repeated measures. The study was done at The Obstetrics and Gynecology Department Immanuel Hospital Bandung, with subjects obtained from the Obstetrics outpatient clinic during the period of May 2008 to March 2009. This study consisted of a population of 66 primigravidas randomly allocated in to 2 groups, 34 women in control group and 32 women in study group. Women in the study group were instructed to wear elastic stockings, while the control group was not. Measurements of veins dilatation were carried out using a Color Duplex Ultrasound. Results: The 12 week examination didn’t show any significant change on left femoral veins diameters in both groups. Examination at 34 weeks gestational age showed significant changes on left femoral veins in study group and control group (subsequently 0,83 ± 0,07 cm and 1,02 ± 0,35 cm). It also happened on examination at 2 weeks post-partum. The incidence of reflux was found only in the study group at sapheno-femoral junction in 6 of 34 women. Conclusion: Mechanical compression provide protective effect from the development of varicoses shown by the reduced incidence in developing reflux in blood flow in saphenous-femoral junction, in addition to minimal dilatation of the lower limbs and pelvic veins. [Indones J Obstet Gynecol 2010;34-1:7-11] Keywords: varicoses; pregnancy; endothelial injury; saphenousfemoral junction; reflux; mechanical compression

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