<![CDATA[Objective: To compare β estrogen receptor on HUVEC endothelial
for each Genistein dose addition with β-estradiol physiologic
dose.
Methode: Experimental study performed in Physiology Laboratorium,
Medical Faculty of Brawijaya University Malang, HUVEC
culture exposed by glucose as a dysfunction endothelial model. Endothel collected from female newborn umbilicus was cultured to
make a confluent culture. The culture was divided into 7 groups: 1.
HUVEC without treatment, 2. HUVEC + glucose 33 mM, 3. HUVEC
+ glucose 33 mM + 17β estradiol , 4. HUVEC + glucose 33
mM+ Genistein 2.5 μm. 5. HUVEC + Glucose 33 mM + Genistein
5 μm, 6. HUVEC + Glucose 33 mM + Genistein 7,5 μm, 7. HUVEC
+ Glucose 33 mM + Genistein 10 μm. Culture then was incubated
in 37°C and stained with immunohistochemistry, using primary antibody of β estrogen receptor. The expression was observed on the
-15, 30, 60, 120 and 240th minute. The datas from observation result was analysed with ANOVA test and correlation test.
Result: Estrogen receptor comparison between group 1 was significantly different with group 2, 3, 6, 7 and not significantly different with groups 4, 5. Estrogen receptor between group 2 was significantly different with group 4, 5 but not significantly different
with groups 3, 6, 7. Estrogen receptor comparison between group 3
was significantly different with group 4, 5 and not significantly different with groups 6, 7. Estrogen receptor comparison between
group 4 was significantly different with group 6, 7 and not significantly different with groups 5. Estrogen receptor comparison between group 5 was significantly different with group 6, 7. Estrogen
receptor comparison between group 6 was not significantly different
with group 7. Isoflavon Genistein dose increase on 30, 60 dan 120
minutes will reduce β estrogen receptor expression on endothelial
HUVEC and on 15 and 240 minutes shows that there was no significant correlation with β estrogen receptor expression on endothel
HUVEC.
Conclusion: There was significant expression distinction of ERβ
in glucose induced HUVEC exposed by 17β estradiol compared with
those exposed by Genistein but not in Genistein dose 7.5 and 10 μm.
The lower of Isoflavon Genistein dose in the -30, 60 dan 120 minute
will cause expression of β estrogen receptor in HUVEC endothelial
cell that tends to be higher.
[Indones J Obstet Gynecol 2010; 34-1:24-30]
Keywords: β estrogen receptor, HUVEC, genistein, estradiol.]]>
