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Berkala Bioteknologi
Published by Universitas Diponegoro
ISSN : -     EISSN : -     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
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Articles 5 Documents
 1 
Search results for , issue "Vol. 5, No. 1, April 2022" : 5 Documents clear
Pengaruh Iradiasi Sinar Gamma TerhadapInduksi Kalus dan Seleksi Tingkat Toleransi Padi (Oryza sativa L.) terhadap Cekaman Salinitas secara In-Vitro Lisdyayanti, Novita Dwi; Anwar, Syaiful; Darmawati, Adriani
Berkala Bioteknologi Vol. 5, No. 1, April 2022
Publisher : Berkala Bioteknologi

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Abstract

The use of saline for rice cultivation needs to be support bytolerant rice varieties in saline land. The rice variety can be obtained through plant breeding programs, one of which is by physical mutation methods using gamma rays.. Physical mutation is random mutation, so we need to do selection after that treatment. Selection to get rice mutants that are tolerant of salinity stress can be done by planting on culture media that have been modified witt addition of NaCl.The perpose of this research is to examine the effect of gamma rays irradiation doses on rice callus induction and its tolerance level in saline media. This research used 2 designs, a monofactor complete randomize design at callus induction stage with a faktor in the form of gamma rays irradiation doses (0, 100, and 200 Gy) and factorial complete randomize designat the regeneration stage with a factor in form of gamma rays irradiation doses (0, 100 and 200 Gy) and the second factor is NaCl concentration (0 and 50 mM). The steps of this research were irradiation of Ciherang variety rice seed, making media for tissue culture, sterilization of rice seed explants, initiation of explants in callus induction media, subculture to regenerated media that had been modified with the addition of NaCl according to treatment, and then observation. The results showed that gamma rays irradiation could inhibit the growth of callus diameter and reduce the grofth of callus from explants of ciherang variety. All callus subcultured into regeneration media are unable to formbuds and turn black or die.
Deteksi Gen DXS dan Penentuan Jalur Biosintesis Karotenoid pada Chlorella pyrenoidosa Monalita, Ramadhebi; Kusumaningrum, Hermin Pancasakti; Budiharjo, Anto
Berkala Bioteknologi Vol. 5, No. 1, April 2022
Publisher : Berkala Bioteknologi

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Abstract

Natural carotenoid synthesis has never exceeded synthetic products on a commercial scale. Lack of understanding of the microbiological and ecophysiological aspects of carotenoid-producing isolates leads to misidentification of species. One local isolate of green algae is used as a natural food source of carotenoids in the food industry, namely Chlorella pyrenoidosa. Carotenoid accumulation of the nonMVA pathway in green algae is determined by the enzyme D-1-deoxyxylulose 5-phosphate synthase, which is encoded by the D-1-deoxyxylulose 5-phosphate synthase (DXS) gene. The main purpose of this study was to detect the DXS gene as a carotenoid biosynthetic key enzyme encoder in C. pyrenoidosa whether or not to follow the non-MVA pathway for carotenoid biosynthesis or not. Morphological and ecophysiological characterization methods are carried out based on periodic observations and DXS gene detection using the guide Kuzuyama (2000). The results of the analysis of the similarity of the C. pyrenoidosa DXS gene in sustainable areas show that it can detect partial DXS genes from Chlamydomonas reinhardtii. The absence of growth inhibition in C. pyrenoidosa with lovastatin shows a non-MVA pathway that is the pathway used in carotenoid biosynthesis.
Potensi Rizobakteri Pembentuk Endospora Dari Tanaman Padi Sebagai Biokontrol Fitopatogen Xanthomonas oryzae Sumarno, Maerani; Budiharjo, Anto; Pujiyanto, Sri
Berkala Bioteknologi Vol. 5, No. 1, April 2022
Publisher : Berkala Bioteknologi

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Abstract

Xanthomonas oryzae is phytopathogen causing bacterial leaf blight which decreases in agricultural product reaching 20-70 % in Asia. Bacterial leaf blight symptoms is characterized by the formation of lines in the leaf blade turnings yellow, then white, causing the plant to wither and die. Endospore-forming rhizobacteria are soil microbes potential as biocontrol to inhibit phytopathogen growth. The aims of this study were to isolate endospore-forming rhizobacteria from rice plant and determine its ability as biocontrol against X. oryzae. The methods used consisted of isolation, antibacterial activity test, molecular identification, and biochemical characterization. Twenty isolates of endospore-forming rhizobacteria were obtained from the isolation of the rice crop. Isolate P-10 had the greatest ability against X. oryzae with inhibition zone of 18.89 mm. Molecular identification using 16S rRNA gene showed that isolates P-10 had 98 % homology with Bacillus pumilus. Biochemical characterization showed the isolate P-10 had a rod- shaped with center of endospores, gram-positive, catalase positive, are motile, negative in starch hydrolyze, not forming gas on glucose, these characteristics fitted with B. pumilus character.
Ekstraksi Dan Uji Stabilitas Zat Warna Daun Jambu Biji (Psidium guajava L.) Pardede, Lasria; Kusdiyantini, Endang; Budiharjo, Anto
Berkala Bioteknologi Vol. 5, No. 1, April 2022
Publisher : Berkala Bioteknologi

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Abstract

Guava leaves is known as a traditional medicine to treat various diseases, such as diarrhea, dengue fever, etc. Along with the development of technology, guava leafs is now used as a color contributor on textiles. This study aims to extract on the solvent distilled water and ethanol and to test the stability of guava leaf color pigment against the influence of storage temperature, time span of the addition of an oxidant and pH. Leaves extracted by soaked for 24 hours in distilled water solvent that has been preheated to 30°C, 50ºC, 70º C and 90ºC and ethanol with a concentration of 20%, 40%, 60%, 80% and 96%. Absorbance measurements of guava leaf extract is using spectrophotometer at a wavelength of 525 nm. The results showed that guava leaf extract has the optimum absorbance value on distilled water solvent at 90ºC and 20%ethanol. Stability test is done by storage temperature effect, oxidizing agents adding, and pH treatment. Stability test of guava leaf extract showed that extracted guava leaf color pigment is stable on 9°C storage temperature
Isolasi dan Identifikasi Molekuler Bakteri Pelarut Fosfat dari Gua Gamelan di Kawasan karst Kiskendo Kendal, Jawa Tengah Mahmudah, Hawari Rosdiana; Suprihadi, Agung; Budiharjo, Anto
Berkala Bioteknologi Vol. 5, No. 1, April 2022
Publisher : Berkala Bioteknologi

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Abstract

Phosphate solubilizing bacteria is a group of bacteria that dissolves unavailable phosphate into the form that can be absorbed by plants. This research aimed at isolation and moleculer identification of phosphate solubilizing bacteria based on 16s rRNA from bat cave guano and potential microorganisms which were able to dissolved phosphate. Several stages of this research are, isolation PSB in Pikovskayaand NBRIP agar, Gram staining, and molecularidentification. The isolation result obtained two isolates phosphate solubilizing bacteria, which are G4-1 and G5. Isolate G5 is the highest on solubilizing phosphate with a diameter of clear zone 22.01 mm, and Isolate G4-1 exhibited diameter of clear zone 19.8 mm. Isolates G4-1 and G5 are the gram-negative bacteria. DNA amplification of these bacteria employing universal primers 27 F (5’-AGAGTTTGATCMTGGCTCAG-3’) dan Primer 1492 R (5’-GGTTACCTTGTTACGACTT-3’) generated the 1520 bp and 1235 bp PCR product. The result of the analysis of 16s rRNA gene sequence showed that isolate G4-1 has 80% similarity with Acinetobacteriwofii strain-JCM partial sequence, and isolate G5 has 92% similarity with Serratia marcesens strain-NBRC.

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