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INDONESIA
SAINS DAN MATEMATIKA
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Articles 5 Documents
Search results for , issue "Volume 19 Issue 4 Year 2011" : 5 Documents clear
Purifikasi DNA Kromosom Geobacillus sp. dYTae-14 Menggunakan Kolom Silika dengan Denaturan Urea Ayu, Budi Putri; Sarjono, Purbowatiningrum Ria; Aminin, Agustina L. N.
JURNAL SAINS DAN MATEMATIKA Volume 19 Issue 4 Year 2011
Publisher : JURNAL SAINS DAN MATEMATIKA

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Studi pemurnian DNA kromosom Geobacillus sp. dYTae-14 menggunakan silika gel dengan denaturan urea telah dilakukan. Pemurnian dilakukan dengan metode adsorpsi silika tanpa kolom dan dengan kolom untuk menentukan metode yang lebih efisien dalam pemurnian DNA kromosom. Metode adsorpsi silika dengan kolom dilakukan dengan sentrifugasi dan tanpa sentrifugasi. Variasi konsentrasi 6 - 9 M urea dengan 25 mg silika gel digunakan untuk mempelajari profil kapasitas urea dalam pemurnian DNA kromosom. Pengukuran tingkat kemurnian dan konsentrasi DNA dianalisis dengan metode spektrofotometri. Hasil menunjukkan bahwa metode adsorpsi silika yang efisien dalam pemurnian DNA kromosom Geobacillus sp. dYTae-14 adalah metode kolom silika dengan sentrifugasi. DNA yang diperoleh pada urea konsentrasi 6 – 9 M memiliki nilai kemurnian sekitar 1,2. Namun, urea 8 M merupakan konsentrasi optimum untuk pemurnian DNA kromosom karena diperoleh kadar DNA yang paling tinggi, yaitu sebesar 811 µg/mL   Keywords: pemurnian DNA kromosom, kolom silika, denaturan, urea.
Fauna Kalajengking (Arachnida : Scorpiones) Di Pemukiman Jatiluhur Jatingaleh Semarang The scorpion fauna ( Arachnida : Scorpiones ) in Semarang Jatingaleh Jatiluhur Settlement Hadi, Mochamad; Rohman, Arief Fatkhu
JURNAL SAINS DAN MATEMATIKA Volume 19 Issue 4 Year 2011
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Lingkungan urban merupakan habitat kompleks yang dikembangkan manusia dari lokasi alami atau lahan pertanian yang secara berangsur-angsur dan sepenuhnya telah mengubah kondisi alami menjadi kondisi tidak alami. Lingkungan urban dan sub-urban menyediakan habitat yang sesuai untuk sejumlah kecil fauna kalajengking. Kalajengking sebagai hewan pemangsa berperan dalam  menjaga keseimbangan ekosistem dan jaring makanan, namun demikian kontak dengan manusia menjadikan keberadaan kalajengking dalam lingkungan urban menjadi dilema tersendiri. Penelitian bertujuan untuk mengkaji keberadaan dan karekteristik habitat kalajengking di lingkungan urban di Jatiluhur, Jatingaleh, Semarang. Teknik pengamatan dilakukan dengan observasi secara langsung. Data pengamatan berupa karakteristik habiitat dijelaskan secara deskriptif. Hasil penelitian telah ditemukan dua spesies kalajengking yaitu Chaerilus variegatus dan Isometrus maculatus. Habitat kalajengking berupa pekarangan rumah, dengan mikrohabitat bebatuan, tumpukan genteng, sisa beton bangunan.  Kata kunci : kalajengking, Chaerilus, Isometrus.  Urban environment is a complex habitat that humans developed from natural or agricultural land locations that gradually and completely has changed the natural conditions into unnatural conditions . Urban environment and sub-urban provide suitable habitat for a small number of scorpion fauna . Scorpions as predators play a role in maintaining the balance of ecosystems and food webs , however, make the existence of human contact with scorpions in the urban environment into a dilemma . The study aims to assess the existence and characteristics of scorpion habitats in urban environments in Jatiluhur , Jatingaleh , Semarang . Engineering observations were made by direct observation . Observational data in the form of descriptive characteristics described habiitat . The results have been found in two species of scorpion that is Chaerilus variegatus and Isometrus maculatus . Keywords : scorpion , Chaerilus , Isometrus
Deposisi Nanopartikel Titanium Dioksida (Tio2) di atas Gelas Transparan Konduktif dan Aplikasinya sebagai Elektroda Kerja pada Sel Surya Berbasis Dye (DSSC) Wahyudi, Bayu; Widiyandari, Hendri
JURNAL SAINS DAN MATEMATIKA Volume 19 Issue 4 Year 2011
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Untuk mengantisipasi krisis energi beberapa dekade ke depan ini, sel surya tersensitasi zat pewarna (dye) telah dipertimbangkan sebagai salah satu sumber energi terbarukan yang sangat potensial. Dalam penelitian ini, telah berhasil dibuat elektroda aktif dari nanopartikel TiO2 yang dideposisikan diatas gelas transparan konduktif TCO dengan metode Doctor blade. Nanopartikel TiO2 yang telah dideposisikan kemudian dikarakterisasi menggunakan SEM (scanning electron microscopy) untuk mengetahui sruktur morfologi permukaan. Performa fotovoltaik dari sel surya DSSC telah diukur berdasarkan nilai voltase sirkuit terbuka (Voc) dan dikorelasikan terhadap rapat arus sirkuit pendek (Jsc) . Dari hasil pengujian terhadap performa DSSC diperoleh efisiensi maksimum dengan Voc = 0.68 V dan Jsc= 4.34 mA/cm2. Kata kunci: DSSC, nanopartikel TiO2, metode Doctor blade, fotovoltaik, efisiensi konversi
Diatom Epipelik sebagai Bioindikator Kualitas Perairan Danau Rawa Pening Soeprobowati, Tri Retnaningsih; Hidayat, Jafron Wasiq; Baskoro, Karyadi
JURNAL SAINS DAN MATEMATIKA Volume 19 Issue 4 Year 2011
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Diatom is a unicellular micro algae that had an important role in the food web and major contributor of oxygen in the water. The short life cycle, rapid reproduction, cosmopolite, wide spread distribution, variation in population, most are sensitive to the environmental changes, easily handle samples and identification, low cost of  sampling and data analysis may promote diatoms as a powerful bioindicator of water quality. The unique siliceous frustules make diatoms able to preserve in the sediment. Rawa Pening is a semi natural lake that administratively surrounded by four districts that consist of 27 villages. The main problem of this lake is erosion in the upstream and sedimentation in the downstream area as well as uncontrolled of aquatic plant growth, particularly water hyacinth (Eichornia crassipes) that induce lake shallowness. For people who live around Rawa Pening, this lake had been used for agricultural irrigation, fisheries, electricity power and tourism. To conserve the lake, as 3rd World Water Forum in Tokyo, March 2003 and 2006 – 2009 National Research Agenda there is a need of limnological research on the environmental changes. This research was conducted in order to study the potential used of epipelic diatom as bioindicator of lenthic ecosystem, particularly Rawa Pening Lake. Water and sediment samples were taken from 27 sites from inlet, outlet and water body of Rawa Pining Lake. There were 254 diatom species that consist of 8 Centrophycidae species and 246 Pennatophycidae species. The population varied between 6,989 and 3,781,000 individual/gram. Based on diversity indices of diatom, some part of Rawapening Lake was unstable, whereas the others were stable based on the diversity indices. Based on epipelic diatom, Rawa Pening Lake and its catchments area might be divided into 3 groups: agricultural lotic ecosystem, settlement lotic ecosystem and lenthic ecosystem. The high population of Synedra ulna, Nitzschia palea and Aulacoseira indicated that the lake is eutrophic that tent to be hypereutrophic. It was supported by high concentration of total nitrogen and phosphorous. The concentration of heavy metals Cadmium, Chromium, Copper and Lead were very high in sediment. However, the government of Indonesia has not yet set up sediment quality criteria. The following research would be proposed on this matter. Key words: diatom, bioindicator, water quality, Rawapening
16S rRNA Gene Analysis of Chlorate Reducing Thermophilic Bacteria from Local Hot Spring Aminin, Agustina L. N.; Katulistiwasari, Puri; Mulyani, Nies Suci
JURNAL SAINS DAN MATEMATIKA Volume 19 Issue 4 Year 2011
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Chlorates waste remediation by biological processes has been the object of current research. Strain CR, the chlorate reducing bacteria was isolated from Gedongsongo hot spring using minimal medium broth containing chlorates and acetate at 55oC. The determination of chlorate reduction from medium was carried out using turbidimetric method. CR isolate showed reducing ability 18% after four days of incubation. The phenotypic character of CR isolate including rod-shaped cells, gram-positive bacteria and facultative anaerobes. On the basis of 16S rRNA analysis, CR isolate was closely related to Bacillus pallidus, Aeribacillus E3, Geobacillus pallidus and Geobacillus sp.D64. The similarity of nucleotide sequences of 16S rRNA gene was 97%, suggested as a novel species.   Keyword: thermophilic bacteria, chlorate reducing, 16S rRNA gene

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