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Biotika: Jurnal Ilmiah Biologi
Published by Universitas Padjadjaran
ISSN : 14124297     EISSN : 26214180     DOI : https://doi.org/10.24198/biotika
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
BIOTIKA Jurnal Ilmiah Biologi adalah Jurnal terbuka yang diterbitkan oleh Departemen Biologi Universitas Padjadjaran yang berdiri sejak tahun 2002. Artikel yang diterbitkan di Jurnal Biotika meliputi semua artikel penelitian asli (original article) yang relevan dengan bidang Biologi dan akan ditelaah secara tertutup oleh mitra bestari. Dalam era interdisipliner ini, Biotika berperan sebagai media komunikasi ilmiah untuk bidang Biologi dan aplikasi terapannya yang relevan, seperti mikrobiologi, genetika dan molekuler, biologi struktur, biologi fungsi, biologi lingkungan maupun biologi terapan. Biotika juga berperan dalam menerbitkan hasil penelitian yang berkualitas dari peneliti muda untuk dapat dijadikan informasi ilmiah bagi pengembangan ilmu pengetahuan dan penelitian. Para kontributor Jurnal Biotika terbuka untuk peneliti dari bidang-bidang terkait, akademisi dan mahasiswa berbagai strata (S1, S2, dan S3). Jurnal BIOTIKA diterbitkan setiap 6 bulan sekali yaitu bulan Juni dan Desember.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 7 Documents
 1 
Search results for , issue "Vol 1, No 1 (2002): Biotika Juni 2002" : 7 Documents clear
FEEDING ACTIVITY AND GROWTH EFFICIENCY OF COMMON CARP (CYPRINUS CARPIO LIN.) EXPOSED TO SYNTHETICS DETERGENT (LAS) Sunardi .. .; Darmadi Goenarso
BIOTIKA Jurnal Ilmiah Biologi Vol 1, No 1 (2002): Biotika Juni 2002
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v1i1.147

Abstract

A study on feeding activity and growth efficiency of common carp (Cyprinus carpio) exposed to synthetics detergent Linear Alkyl Sulphonate (LAS) has been conducted. The period of the experiment was 30 days with 6-day observation interval. Fishes were tested in three different concentrations of LAS i.e. 0.5, 1.5, and 4.5 ppm. Feeding rate (FR), absorption efficiency (AE), production rate (PR) and growth efficiency (GE) were measured. The results showed that the increased of LAS concentration decreased FR from 51.74 (control) to 40.27 mg.g bw-1.d-1 (22.17 %) in treated fish. LAS also reduced AE from 94.88% to 87.84% (6.89%). In addition, LAS decreased PR from 2.70 to 1.03 mg.g bw-1.d-1 (61.85%0 and GE from 5.21 to 2.58% (50.48%).
KEJADIAN PERDARAHAN DAN KELAMIN GANDA PADA EMBRIO PENYU HIJAU (Chelonia mydas) YANG DIDEDAHKAN TERHADAP HERBISIDA PARAKUAT DIKLORIDA Yetty Yusri Gani; Pipit Pitaloka
BIOTIKA Jurnal Ilmiah Biologi Vol 1, No 1 (2002): Biotika Juni 2002
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v1i1.159

Abstract

This experiments had been carried out in our laboratory to learn wether paraquat herbisides were responsible to disrupt the developmental profile and sex differentiation of green turtle (Chelonia mydas) embryos. Paraquat dichlorides which were 22; 31; 42; 58 mg/kg of sand weight sprayed to the incubation sand covering fertile eggs for the incubation period between the day 19th (Stage 18) and 43th (Stage 24). Tap water without paraquat also sprayed as control. On the observable embryos were significantly found hemorrhages and gonadal intersex status. Hemorrhages embryos were  60% to 100% occurred in all treated paraquat, while 20% in the control. Statisticaly the hemorrhages were significant (p £ 0,05) in 22; 31; and 42 mg/kg, and highly significant (p £ 0,01) in 58 mg/kg of paraquat, were showed these percentage increasing depend in the given concentration (r = 0,9387). The gonadal intersex of green turtle embryos were signated by well developed both in cortical and medullary as ovary and testicle anlage, and occurred in 31; 42; and 58 mg/kg of paraquat. Since the presence of paraquat had been detected in the allantoic fluid of embryos, ensure that paraquat absorbed into embryos and this herbicides might have lead to the disruption of embryos directly. Key words : Paraquat dichlorides, Green turtle (Chelonia mydas) embryos, hemorrhages, intersex. 
MUTAGENISITAS PARAKUAT DIKLORIDA PADA SEL SPERMATID MENCIT SWISS-WEBSTER (Mus musculus) DENGAN UJI MIKRONUKLEUS Cucu Hadiansyah; Siskasari Polianti
BIOTIKA Jurnal Ilmiah Biologi Vol 1, No 1 (2002): Biotika Juni 2002
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v1i1.153

Abstract

Research on the mutagenicity of paraquat to the spermatid of Swiss-Webster mice had been  studied  by micronucleus assay.  The doses of paraquat, which were  17;  25,5;  34 mg/kg bw, physiological saline as negative control, and 40 mg/kg bw of cyclophosphamide as positive control, each administered intraperitoneally at six consecutive times to male Swiss-Webster mice. The spermatid samples taken 16 days later since the first treatment by cell masseration method in trypsin and DNase solution, to formed  germ cells suspension.  Cells  suspension  were  filtered  (mesh  no. 325) and centrifuged (100 rpm, 5 minutes) then the pellet were smeared and stained by Giemsa staining. The micronucleus in spermatid (MN-spermatid) were observed in these preparation as a dark blue spherical or ellipsoidal node with size among 1/5 – 1/3 to the nucleus diameter and counted per 500 spermatid per animal. The frequencies of MN-spermatid observed after having induced by paraquat was significant (p<0.05) at the dose of 25.5 mg/kg bw and highly significant (p<0.01) at the dose of 34 mg/kg bw to the negative control.  The induction of micronucleus by paraquat had a positive correlation  to  the given doses. On this research, 34 mg/kg bw of paraquat induced 12.0 ± 2.45‰ were similar (p<0.01) to the induction of 40 mg/kg bw of cyclophosphamide (15.6 ± 2.00‰), these indicated that paraquat had a mutagenic effect to the mice spermatid as same as a level of cyclophosphamide mutagenicity.Research on the mutagenicity of paraquat to the spermatid of Swiss-Webster mice had been  studied  by micronucleus assay.  The doses of paraquat, which were  17;  25,5;  34 mg/kg bw, physiological saline as negative control, and 40 mg/kg bw of cyclophosphamide as positive control, each administered intraperitoneally at six consecutive times to male Swiss-Webster mice. The spermatid samples taken 16 days later since the first treatment by cell masseration method in trypsin and DNase solution, to formed  germ cells suspension.  Cells  suspension  were  filtered  (mesh  no. 325) and centrifuged (100 rpm, 5 minutes) then the pellet were smeared and stained by Giemsa staining. The micronucleus in spermatid (MN-spermatid) were observed in these preparation as a dark blue spherical or ellipsoidal node with size among 1/5 – 1/3 to the nucleus diameter and counted per 500 spermatid per animal. The frequencies of MN-spermatid observed after having induced by paraquat was significant (p<0.05) at the dose of 25.5 mg/kg bw and highly significant (p<0.01) at the dose of 34 mg/kg bw to the negative control.  The induction of micronucleus by paraquat had a positive correlation  to  the given doses. On this research, 34 mg/kg bw of paraquat induced 12.0 ± 2.45‰ were similar (p<0.01) to the induction of 40 mg/kg bw of cyclophosphamide (15.6 ± 2.00‰), these indicated that paraquat had a mutagenic effect to the mice spermatid as same as a level of cyclophosphamide mutagenicity.
DISTRIBUSI DAN DAERAH JELAJAH LUTUNG (Trachypithecus auratus sondaicus) DI TAMAN WISATA ALAM PANGANDARAN Teguh Husodo; Erri Noviar Megantara
BIOTIKA Jurnal Ilmiah Biologi Vol 1, No 1 (2002): Biotika Juni 2002
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v1i1.161

Abstract

ABSTRACTThe study distribution and population of lutung(Trachypithecus auratus sondaicus) in Pangandaran NaturalRecreational Park (TWAP) was conducted in January 2001through census method.  Broad inventory observation wasapplied to identified plant community and dominant plantspecies. There are 12 lutung groups and evenly distributed  in 37,7 ha area of TWAP,  thus the  density  of  lutung  is rangebetween 35-36 groups/km2. Population average on groups as13,1-13,5, 20-21 are maximized and  5-6 are minimizingpopulation.  Composition  of  sex  ratio  average  on  eachgroup  was  1,9 adult  male/group,  6,9-7,5 adult female/group,  2 subadult/group,   2,7-3,2 young/group and1 infant/group. Lutung habitat on TWAP were secondaryforest, with dominance vegetation are laban (Vitexpubescens), kisegel (Dillenia exelsa) and marong(Cratoxylum formosum), nyamplung (Callophylluminophylum), ketapang (Terminalia catappa), waru (Hibiiscustiliaceus) and butun (Barringtonia asiatica). Recorded 40 of 60species have 1 % frequency index were lutung food.  Duringthe studies  members  splitter  recorded  from  their  group, like A, B and C groups  to  A1-1, A1-2, B1, B2, C2-1 and C2-2.  Average  of  territory area  of all groups were 2,78 ha, 6,67ha are maximized and 2,78 ha are minimized.Keyword :Lutung, distribution, homerange, population, PananjungPangandaran Nature Reserve.
PENGARUH SUHU DAN EKSTRAK BIJI NIMBA (Azadirachta indica A. Juss) TERHADAP JUMLAH HEMOSIT JENGKERIK (Gryllus mitratus Burm.) Hikmat Kasmara; Ahmad Ridwan; Darmadi Goenarso
BIOTIKA Jurnal Ilmiah Biologi Vol 1, No 1 (2002): Biotika Juni 2002
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v1i1.155

Abstract

A B S T R A C T   The effect of temperature and neem seed extract (Azadirachta indica A. Juss) on the haemocyte number of cricket (Gryllus mitratus Burm.) has been studied. Temperatures applied in this experiment were 25o ± 1o C, 30o ± 1o C and 35o ± 1o C. Neem seed extract was injected in volumes of 10 ml/g body weight into the ventral abdomen intersegment between segment 5 and 6 of the insect body. The concentrations of neem seed extract were 0.0 %, 2.5 %, 5.0 %, 10.0 % and 20.0 %. Controls without injection were also analysed. Hemolymph was taken by sterile needle from the prothorax. The experiment design was randomized block design (RBD) factorial pattern. In this experiment, four types of haemocyte were observed e.g. prohaemocyte (PR), plasmatocyte (PL), coagulocyte (CO) and granulocyte (GR). At 25o C, treatment of 0.0 %, 2.5 % and 5.0 % neem’s seed extract to increased the haemocyte number during 3 hours exposure, at 10.0 % neem seed extract the haemocyte number started to decrease, while at 20.0 % the haemocyte number returned to the same level as the control. The same pattern was obtained for the treatment at 30o and 35o C. When the exposure was increased to 24 hours, at 25o C, application of neem seed extract 0.0 %, 2.5 %, 5.0 %, 10.0 % and 20.0 % increased haemocyte number compared to control. The differences of the haemocyte number between control and treatment at 30o C, only occurred on the 5.0 % neem seed extract. At 35o C, the haemocyte number of the 0.0 %, 2.5 % and 5.0 % neem seed extract were equal to control, while on the 10.0 % and 20.0 %, the haemocyte number were lower than control. The lowest haemocyte number was obtained at 35o C, 20.0 % neem seed extract and 24 hours exposure. Key words : temperature, neem seed extract, haemocyte, cricket
FEEDING DETERRENT ACTIVITY OF ANDROGRAPHOLIDE AGAINST COTTON BOLLWORM , HELICOVERPA ARMIGERA HüBNER (LEPIDOPTERA : NOCTUIDAE) Wawan Hermawan
BIOTIKA Jurnal Ilmiah Biologi Vol 1, No 1 (2002): Biotika Juni 2002
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v1i1.143

Abstract

In laboratory choice and no-choice tests, andrographolide was tested against the 4thinstar larvae of the cotton bollworm (CBW), Helicoverpa armigera using cabbage leaf disc. Each test was repeated 5 times. No-choice test showed that andrographolide was less effective in feeding suppression against CBW. In choice test, andrographolide significantly suppressed feeding at high dose of 400mg/leaf disc. Key words : Helicoverpa armigera, andrographolide, feeding deterrent.
ISOLASI PROTOPLAS TANAMAN KACANG HIJAU (Vigna radiata (L.) Wilczek) SECARA ENZIMATIK DENGAN KOMPOSISI ENZIM BERBEDA Mohamad Nurzaman; Tia Setiawati
BIOTIKA Jurnal Ilmiah Biologi Vol 1, No 1 (2002): Biotika Juni 2002
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v1i1.149

Abstract

The effects of different enzyme compositions and age of explants on protoplasts isolation  of mungbean (Vigna radiata (L) Wilczek) had been carried out. Protoplasts were isolated from the first leaves of 5, 6, 7, and 8 days old of mungbean seedling, which were incubated in 5 types enzyme composition for 4 hours. The results showed that the yield of protoplasts more than 106 protoplas/g leaf for all combinations. The highest yield of  protoplasts (3,4 x 107 protoplasts/g leaf) were obtained from 7 days old seedling using a combination of 4% Meicelase P-1, 1% Macerozyme R-10 and 0.5 M mannitol. From these results, it can be concluded that the type of enzyme compositions and age of explants influent the yield of protoplasts isolation

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