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All Journal Jurnal Biologi Udayana Biotika: Jurnal Ilmiah Biologi Indonesian Journal of Applied Sciences AL KAUNIYAH
Tia Setiawati
Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Materials Science & Nanotechnology

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ISOLASI PROTOPLAS TANAMAN KACANG HIJAU (Vigna radiata (L.) Wilczek) SECARA ENZIMATIK DENGAN KOMPOSISI ENZIM BERBEDA Mohamad Nurzaman; Tia Setiawati
BIOTIKA Jurnal Ilmiah Biologi Vol 1, No 1 (2002): Biotika Juni 2002
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v1i1.149

Abstract

The effects of different enzyme compositions and age of explants on protoplasts isolation  of mungbean (Vigna radiata (L) Wilczek) had been carried out. Protoplasts were isolated from the first leaves of 5, 6, 7, and 8 days old of mungbean seedling, which were incubated in 5 types enzyme composition for 4 hours. The results showed that the yield of protoplasts more than 106 protoplas/g leaf for all combinations. The highest yield of  protoplasts (3,4 x 107 protoplasts/g leaf) were obtained from 7 days old seedling using a combination of 4% Meicelase P-1, 1% Macerozyme R-10 and 0.5 M mannitol. From these results, it can be concluded that the type of enzyme compositions and age of explants influent the yield of protoplasts isolation
DIVERSITAS GENETIK KERABAT LIAR UBI JALAR IPOMOEA TRIFIDA (H.B.K.) G.DON. BERUMBI ASAL CITATAH JAWA BARAT BERDASARKAN KARAKTER KROMOSOM (Genetic Diversity Of Wild Relative Of Sweet Potato Tubered-Ipomoea Trifida (H.B.K.) G.don. Originated From Citatah West Java Based On Chromosome Characters) Tia Setiawati
Indonesian Journal of Applied Sciences Vol 3, No 3 (2013)
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4049.419 KB) | DOI: 10.24198/ijas.v3i3.15053

Abstract

AbstrakKerabat liar ubi jalar Ipomoea trifida asal Citatah Jawa Barat potensial digunakan dalam program pemuliaan tanaman ubi jalar (Ipomoea batatas (L.) Lam.) Untuk mengetahui diversitas genetik I. trifida  dilakukan pengamatan terhadap karakter kromosom. Bahan tanaman yang digunakan  adalah 10 spesies I. trifida berumbi. Hubungan kekerabatan antar spesies diketahui melalui analisis klaster dan Analisis Komponen Utama/Principal Component Analysis (PCA). Hasil pengamatan menunjukkan pengujian pada 10 aksesi I. trifida berumbi dengan menggunakan 9 karakter kromosom menghasilkan jarak ketidakmiripan (Euclidean coeffisien) yang berkisar 1,75 – 6,22 dan menunjukkan diversitas yang luas. Dendogram yang dihasilkan pada jarak ketidakmiripan 5,23 menunjukkan terbentuknya 3 klaster utama. Analisis Komponen Utama (PCA) menghasilkan 2 komponen utama pertama (PC1 dan PC2) yang telah dapat menjelaskan 89,64% dari total variasi. Kata kunci : Ipomoea trifida, Analisis Klaster, Analisis Komponen UtamaAbstractWild relatives of sweet potato, Ipomoea trifida originated from Citatah West Java was potential use in plant breeding programs of Ipomoea batatas (L.) Lam. To determine the genetic diversity of I. trifida, observation on characters of chromosomes of I.trifida was conducted. Plant materials used ten accessions  of tubered-I. trifida. Relationship between species identified by cluster analysis and Principal Component Analysis (PCA). The results showed that the observation on the 10 accession of tubered-I. trifida using 9 characters chromosome produces dissimilarities distance (Euclidean coefficient) ranging from 1.75 to 6.22. Dendogram generated  at a dissimilarity distance of 5.23 showed the formation of three main clusters. Principal Component Analysis (PCA) produced first two principal component (PC1 and PC2), which has been able to explain 89.64% of the total variation.Keyword  : Ipomoea trifida, Clustering Analysis, Principal Component Analysis
Struktur morfologis dan histologis ginjal tikus model diabet setelah diberi ekstrak etanol kulit buah jengkol (Archidendron pauciflorum) Desak Made Malini; Nurullia Fitriani; Adnin Laila; Nining Ratningsih; Tia Setiawati
Jurnal Biologi Udayana Vol 25 No 2 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i02.p13

Abstract

In a long term, diabetes mellitus (DM) leads to nephropathy due to glomerular hyperfiltration. One of the plant used as a diabetic drug by the community in Karangwangi Village, Cianjur Regency, West Java is the fruit peel of jengkol. Therefore, this study aims to determine the effect of the ethanolic extract of Jengkol fruit peel (EEJFP) toward the morphological and histological structure on the kidney of the diabetic rat model. The method adopted was the Randomized Design (CRD) with 6 treatments namely NC (Carboxyl Methyl Cellulose (CMC) 0.5%), PC (CMC 0.5%), Pb (Glibenclamide 5 mg/kg BW), P1, P2, and P3 (EEJFP 385; 770; and 1,540 mg/kg BW) with 4 replications for 14 consecutive days. Furthermore, the induction of diabetes with streptozotocin dose of 60 mg/Kg BW was performed intravenously in experimental animals except for the NC group. The parameters observed include relative weight, morphological, and histological structure of kidney which include glomerular diameter, Bowman space distance, and percentage of proximal tubular cell necrosis. The non-parametric and parametric data were tested by Kruskal Wallis and ANOVA test as well as Duncan's follow-up test, respectively. The results showed that there was no significant difference in the morphological structure of the kidney between treatment groups. Furthermore, the relative weights of kidney in the PC, Pb, P1, and P3 groups were larger and significantly different compared to NC and P2 also, the histological structure showed that the glomerular diameter (65.43 ± 0.7 m), Bowman space distance (4.19 ± 1.7 µm), and the percentage of proximal tubular cell necrosis (24.6 ± 5.5%) at P2 were not significantly different from NC. Based on this results, it was concluded that EEJFP has no effect on the kidney’s morphological structure, however, it decreases its relative weight and repair the kidney’s histological damage of the diabetic rat model with the optimum dose of 770 mg/kg BW.
PENGARUH POLYETHYLENE GLYCOL (PEG) TERHADAP KADAR KUERSETIN KULTUR KALUS Chrysanthemum morifolium Ramat PADA KONDISI PENCAHAYAAN BERBEDA Tia Setiawati; Syifa Fauzia Zazuli; Annisa Annisa; Mohamad Nurzaman; Budi Irawan
Al-Kauniyah: Jurnal Biologi Vol 13, No 1 (2020): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3713.676 KB) | DOI: 10.15408/kauniyah.v13i1.13688

Abstract

AbstrakKrisan (Chrysanthemum morifolium Ramat) mengandung senyawa kuersetin dengan efek farmakologi yang sangat luas. Penambahan polyethilene glycol (PEG) dapat dimanfaatkan untuk meningkatkan produksi metabolit sekunder secara in vitro. Penelitian ini bertujuan untuk mengetahui pengaruh PEG terhadap pertumbuhan kalus krisan dan kadar kuersetin 3-O-rhamnosida pada pencahayaan yang berbeda. Penelitian menggunakan Rancangan Acak Lengkap (RAL). Eksplan kalus berumur 45 hari setelah tanam (HST) disubkultur pada media MS + 4 ppm 2,4-D dengan penambahan PEG dalam lima taraf konsentrasi yaitu 0, 10, 20, 30, dan 40 ppm. Kultur diinkubasi pada kondisi gelap dan terang. Parameter yang diamati adalah warna, tekstur, ukuran, berat basah, dan berat kering kalus serta kadar kuersetin 3-O-rhamnosida. Data kuantitatif dianalisis menggunakan Analisis Varians dan Uji Jarak Berganda Duncan (α 5%), sedangkan data kualitatif dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa kalus pada kondisi terang berwarna cokelat dan cokelat kehijauan, sedangkan pada kondisi gelap berwarna putih kecokelatan. Perlakuan 10 ppm PEG menghasilkan berat basah dan berat kering kalus tertinggi baik pada kondisi terang maupun gelap berturut-turut 1,97 g dan 2,92 g; 0,94 g dan 1,09 g. Kadar kuersetin 3-O-rhamnosida tertinggi pada kondisi gelap dan terang terdapat pada perlakuan 10 ppm PEG berturut-turut  1,72 µg/g berat kering (BK) dan 2,59 µg/g BK.Abstract Chrysanthemum morifolium Ramat. contains quercetin with very broad pharmacological effects. The addition of PEG can be used to increase the production of secondary metabolites using in vitro method. This study aimed to determine the effect of PEG on the growth of Chrysanthemum callus and quercetin 3-O-rhamnoside content in different lighting conditions. The experimental design used was a Completely Randomized Design. After 45 days, callus were subcultured on MS medium + 4 ppm 2,4-D which PEG was added in five concentration levels (0, 10, 20, 30, and 40 ppm). Culture was incubated in dark and light conditions. Parameters observed were color, texture, size, wet weight and dry weight of callus, also quercetin 3-O-rhamnoside levels. Quantitative data was analyzed using Analysis of Variance and Duncan's Multiple Distance (α 5%). Qualitative data was analyzed descriptively. The results showed that in light condition, the callus has brown and greenish brown color, whereas in dark, it has brownish white color. The 10 ppm PEG treatment produced the highest fresh weight and dry weight in both light and dark conditions of 1.97 g and 2.92 g, 0.94 g and 1.09 g, respectively.The highest quercetin 3-O-rhamnoside content  in dark and light conditions were founded in 10 ppm PEG treatment of 1.72 µg/g dry weight (DW) and 2.59 µg/g DW.
Co-Authors Adnin Laila Annisa Annisa Budi Irawan Desak Made Malini Mohamad Nurzaman Nining Ratningsih Nurullia Fitriani Syifa Fauzia Zazuli