cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Hayati Minarsih
Contact Email
menaraperkebunanppbbi@gmail.org
Phone
-
Journal Mail Official
menaraperkebunan@iribb.org
Editorial Address
Jalan Taman Kencana No.1 Bogor 16128, Jawa Barat
Location
Kab. bogor,
Jawa barat
INDONESIA
Menara Perkebunan
Published by Pusat Penelitian Kelapa Sawit
ISSN : 01259318     EISSN : 18583768     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Menara Perkebunan as a communication medium for research in estate crops published articles covering original research result on the pre- and post-harvest biotechnology of estate crops. The contents of the articles should be directed for solving the problems of production and/or processing of estate crops of smallholder, private plantations and state-owned estates, based on the three dedications of plantation. Analyses of innovative research methods and techniques in biotechnology, which are important for advancing agricultural research. Critical scientific reviews of research result in agricultural and estate biotechnology.
Arjuna Subject : -
Articles 3 Documents
 1 
Search results for , issue "Vol 72, No 1: Juni 2004" : 3 Documents clear
Deteksi dan analisis sekuen gen inhibitor proteinase pada beberapa klon kakao harapan tahan penggerek buah kakao dari Sulawesi Selatan Detection and sequence analysis of proteinase inhibitor gene in cacao clones putatively cacao pod borer-tolerant from South Sulawesi Abdul Mollah S. JAYA; Hajrial ASWIDINNOOR; Djoko SANTOSO
E-Journal Menara Perkebunan Vol 72, No 1: Juni 2004
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (238.784 KB) | DOI: 10.22302/iribb.jur.mp.v72i1.124

Abstract

Summary Cacao is socially and economically an important commodity for Indonesia, in which the cacao plantations have been challenged with a threatening pest, cacao pod borer (CPB). This research aimed to identify and clone PIN (proteinase inhibitor), a gene carrying resistance of plant to some chewing pests like CPB. The methodology included several experiments. Detection of PIN in cacao was done by PCR using PIN-specific heterologous primers and cacao genomic DNA as templates. Cloning vector pGEM-T was utilized to clone the PCR products. Sequence analysis was conducted with BlastX and Blast Special programs from NCBI. Alignment analysis to determine genetic similarity was performed with ClustalW from EBI. Thirteen of the 18 clones tested were detected to have PIN homologs. Two DNA fragments from cacao clones putatively tolerant to CPB, MJ-1 and LW-1, were sequenced. One of them, MJ-1 was cloned. Sequence analyses of the fragments of both cacao clones, indicated that they have PIN homologs and a very closed genetic relation with 96% level of similarity. Ringkasan Kakao adalah komoditas yang secara sosial maupun ekonomi penting bagi Indonesia, dimana perkebunan kakao menghadapi masalah serius hamapenggerek buah kakao (PBK). Penelitian ini bertujuan mengidentifikasi dan mengklon PIN (inhibitor proteinase), gen yang membawa sifat ketahanan tanaman terhadap hama ulat seperti PBK. Metodologinya terdiri dari beberapa percobaan. Deteksi PIN di dalam kakao dikerjakan dengan PCR menggunakan primer heterologous yang spesifik terhadap PIN dan DNA genomik kakao sebagai templetnya. Vektor kloning pGEM-T digunakan untuk mengklon produk PCR. Analisis sekuen dilakukan dengan program BlastX dan Blast spesial dari NCBI. Analisis penjajaran (alignment) untuk menentukan kemiripan genetik menggunakan program ClustalW dari EBI. Tiga belas dari 18 klon kakao yang diuji  menunjukkan adanya  homolog  PIN. Dua DNA fragmen dari klon harapan tahan, MJ-1 dan LW-1, telah ditentukan sekuen nukleotidanya. Satu diantara-nya, MJ-1 berhasil diklon. Analisis sekuen  kedua klon tersebut menunjukkan identitas sebagai homolog PIN dan keduanya memiliki kemiripan genetik yang tinggi.
Perbanyakan tanaman kina Cinchona ledgeriana Moens. dan C. succirubra Pavon melalui penggandaan tunas aksiler Propagation of cinchona plant Cinchona ledgeriana Moens. and C. succirubra Pavon through axillary buds multiplication . JOKO-SANTOSO; Nurita TORUAN-MATHIUS; U SASTRAPRAWIRA; G SURYATMANA; D SAODAH
E-Journal Menara Perkebunan Vol 72, No 1: Juni 2004
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (292.571 KB) | DOI: 10.22302/iribb.jur.mp.v72i1.125

Abstract

SummaryCinchona ledgeriana (Ledger) and C. succirubra (Succi) were industrial commodities which their barks of the trunk  contain alkaloid   used as raw materials in pharmaceutical, food, drug and beverages and chemical industries. The problem  faced in conventional plant propagation are. incompatibilities, high numbers of death caused by transportation, limited numbers and time consume in  plant materials  production. These problems may be  overcome by axillary buds multiplication.  The aim of the experiment were to find out propagation technology of Ledger and Succi by  tissue culture technique.  Experiments were conducted in three consecutive steps, viz the effect of (i) BAP on multiplication and growth of axillary’s bud of Ledger and Succi in vitro culture, (ii) IBA on root initiation and growth, (iii)  growth medium on the growth of plantlets in  acclimatization.The design of the experiments were Complete Randomized Design with 15 (i & ii) and four (iii) replications. The treatments were (i) 0,1,2,3,4, dan 5 mg/L BAP, (ii) 0.0; 0.5; 1.0; 1.5; 2.0; dan 2.5 mg/L IBA, and (iii) mixture of soil and rice husk charcoal (1:1), mixture of soil and compost (1:1),  mixture of soil, rice husk charcoal, and  compost  (1:1:1).  Parameters measured in the experiments were (i) the initiation of buds multiplication rate twice at axillary buds at subculture.  (ii) initiation  and  roots vigor. (iii) numbers of survived  plants and plants vigor. The explant source used derived from two-month old axillary buds cultured in Murashige and Skoog (MS) medium without growth regulator. Results of the experiment showed  that the best shoot multiplication of Ledger  and Succi  was obtained from the application of 3 mg/L BAP, with buds multipli-cation rate 7 buds/explant/month for Ledger, and 3-4 buds/explants/month for succi. The best root initation and root growth were found from the application of 2 mg/L IBA. The highest percentage of survived plantlets (100%) in acclimatization was obtained from mixture of soil and rice husk charcoal (1:1) medium.  Therefore it is  concluded that tissue culture technique could be used for planlet  mass propagation    of  elite C. Ledgeriana and C. Succirubra through axillary bud multiplication.Ringkasan Tanaman kina Cinchona ledgeriana (Ledger) dan C. succirubra (Succi)  merupakan tanaman industri yang mengandung alkaloid di dalam kulit batangnya dan berguna dalam bidang industri farmasi, makanan, minuman dan kimia. Kendala yang dihadapi dalam perbanyakan tanaman kina secara konvensional dengan sistem sambung  adalah inkompatibilitas,    kematian akibat pengangkutan cukup tinggi, jumlah bahan tanam yang diproduksi sangat terbatas dan waktu penyediaan yang cukup lama. Masalah tersebut dapat diatasi dengan menggunakan teknik kultur jaringan. Penelitian ini bertujuan untuk men-dapatkan teknologi perbanyakan tanaman kina Ledger dan Succi dengan teknik kultur jaringan. Penelitian terdiri atas (i) pengaruh BAP terhadap inisiasi dan penggandaan  tunas aksilar, (ii) pengaruh IBA terhadap inisiasi serta pertum-buhan akar planlet,   dan (iii) pengaruh beberapa medium terhadap pertumbuhan planlet dalam aklimatisasi. Percobaan menggunakan Rancangan Acak Lengkap, masing-masing diulang 15 (i & ii)  dan (iii) empat kali. Peubah yang diukur untuk percobaan (i) adalah waktu inisiasi tunas dan laju penggandaan tunas aksiler pada dua  kali  subkulur. (ii)  Waktu  inisiasi  dan vigor akar. (iii) Jumlah tanaman yang bertahan hidup setelah aklimatisasi, serta vigor tanaman. Sumber eksplan yang digunakan adalah tunas aksilar dari kecambah terpilih berumur dua bulan yang dikulturkan dalam medium Murashige dan Skoog tanpa zat pengatur tumbuh. Perlakuan untuk percobaan (i) adalah 0,0; 1,0; 2,0; 3,0; 4,0 dan  5,0 mg/L BAP, (ii) adalah 0,0; 0,5; 1,0; 1,5; 2,0; dan 2,5 mg/L IBA, sedang (iii) adalah medium tanam tanah, tanah : arang sekam (1:1),  tanah : kompos (1:1), tanah : arang sekam : kompos (1:1:1). Hasil yang diperoleh menunjukkan bahwa konsentrasi BAP terbaik untuk inisiasi dan penggandaan tunas tanaman kina Ledger dan Succi adalah 3 mg/L BAP, dengan laju penggandaan tujuh tunas/eksplan/bulan untuk Ledger dan 3-4 tunas/eksplan/bulan untuk Succi. Sedang untuk perakaran diperoleh dari medium MS dengan penambahan 2 mg/L IBA. Persentase tertinggi planlet (100%) yang mampu bertahan hidup pada aklimatisasi diperoleh dari medium campuran tanah : arang sekam (1:1). Berdasarkan hasil tersebut di atas dapat disimpulkan bahwa perbanyakan tanaman kina secara in vitro untuk menghasilkan bibit bermutu dapat dilakukan melalui teknik penggandaan tunas aksiler
Kloning parsial gen penyandi enoil-ACP reduktase dari mesokarp buah kelapa sawit (Elaeis guineensis Jacq.) Partial cloning of gene encoding enoyl-ACP reductase from mesocarp of oil palm (Elaeis guineensis Jacq.) Asmini BUDIANI; Djoko SANTOSO; Hajrial ASWIDINNOOR; Antonius SUWANTO
E-Journal Menara Perkebunan Vol 72, No 1: Juni 2004
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (301.327 KB) | DOI: 10.22302/iribb.jur.mp.v72i1.126

Abstract

Summary Enoyl-ACP reductase (ENR) is a component of fatty acid synthase (FAS) that is considered to play an important role in fatty acid elongation and oil accumulation of several plants. One of the proteins expressed coinciding with fruit development and oil accumulation in oil palm has been detected from the previous study and had homology with ENR. Therefore, as a part of genetic engineering program to improve oil content and quality in oil palm mesocarp, this research was aimed to clone cDNA conserved region of gene encoding enoyl-ACP reductase from oil palm mesocarp. Based on the amino acid sequence of the polypeptide that was homologous with ENR and combined with information of conserved region sequences of the same gene from other plant sources, primers were designed for amplifying conserved region of the ENR gene. Amplifi-cation was carried out by RT-PCR using total RNA as template, at several annealing temperatures and MgCl2 concentrations. Amplification product was cloned using pCR 2.1-TOPO, and the sequence was subjected into BlastN analysis. The results confirmed that the cloned cDNA fragment with 698 bp in size was the conserved region of the ENR gene.  This sequence was highly homologous with the same gene from other plants such as Oryza sativa, Olea europaea, Brassica napus, Triticum aestivum and Arabidopsis thaliana with E-value 1e-96, 7e-77, 2e-64, 5e-41 and 3e-36, respectively. Based on this result, primers have been made and used to amplify the 5’- and 3’ ends of the ENR -cDNA  of oil palm mesocarp. Ringkasan Enoil-ACP reduktase (ENR) merupakan salah satu komponen asam lemak sintase (FAS) yang berperan penting dalam pemanjangan asam lemak dan akumulasi minyak pada berbagai tanaman. Salah satu protein yang ter-ekspresi sejalan dengan tahapan perkembangan buah sawit dan akumulasi minyak pada penelitian sebelumnya diketahui mempunyai homologi dengan ENR. Oleh karena itu, sebagai salah satu bagian dari usaha rekayasa metabolisme minyak pada mesokarp buah sawit, penelitian ini bertujuan untuk mengklon cDNA daerah konservatif gen penyandi ENR dari mesokarp buah sawit. Berdasarkan  sekuen asam amino polipeptida yang mempunyai homologi dengan ENR dan dikombinasikan dengan hasil penjajaran daerah konservatif gen tersebut dari berbagai anaman lain, dirancang primer  untuk  amplifikasi daerah konservatif ENR. Amplifikasi dilakukan dengan RT-PCR menggunakan templat RNA total pada berbagai suhu   penempelan   dan   konsentrasi    MgCl2. Hasil amplifikasi dimurnikan dari gel dan diklon menggunakan vektor kloning pCR2.1-TOPO serta dianalisis nya menggunakan BlastN. Hasilnya mengkonfirmasi fragmen cDNA terklon berukuran 698 pb sebagai daerah konservatif ENR tersebut mempunyai homologi tinggi dengan gen yang sama dari    O. sativa,  O. europaea, B. napus, T. aestivum dan  A. thaliana masing-masing dengan E-value 1e-96, 7e-77, 2e-64, 5e-41 dan 3e-36. Berdasarkan hasil tersebut telah dibuat primer spesifik untuk amplifikasi cDNA daerah ujung 5’- dan 3’- gen  ENR dari mesokarp kelapa 

Page 1 of 1 | Total Record : 3

 1 

Filter by Year

2004 2004


Reset
Filter By Issues
All Issue Vol. 93 No. 1 (2025): 93(1), 2025 Vol. 92 No. 2 (2024): 92(2), 2024 Vol. 92 No. 1 (2024): 92(1), 2024 Vol. 91 No. 2 (2023): 91 (2), 2023 Vol. 91 No. 1 (2023): 91 (1), 2023 Vol. 90 No. 2 (2022): 90 (2), 2022 Vol. 90 No. 1 (2022): 90 (1), 2022 Vol. 90 No. 2 (2022): Oktober, 2022 Vol 90, No 2 (2022): Oktober, 2022 Vol 90, No 1 (2022): April, 2022 Vol. 89 No. 2 (2021): 89 (2), 2021 Vol. 89 No. 1 (2021): 89 (1), 2021 Vol 89, No 2 (2021): Oktober, 2021 Vol 89, No 1 (2021): April, 2021 Vol. 88 No. 2 (2020): 88 (2), 2020 Vol. 88 No. 1 (2020): 88 (1), 2020 Vol 88, No 2 (2020): Oktober,2020 Vol 88, No 1 (2020): April, 2020 Vol. 87 No. 2 (2019): 87 (2), 2019 Vol. 87 No. 1 (2019): 87 (1), 2019 Vol 87, No 2 (2019): OKTOBER, 2019 Vol 87, No 1 (2019): April, 2019 Vol. 86 No. 2 (2018): 86 (2), 2018 Vol. 86 No. 1 (2018): 86 (1), 2018 Vol 86, No 2 (2018): Oktober 2018 Vol 86, No 1 (2018): April, 2018 Vol. 85 No. 2 (2017): 85 (2), 2017 Vol. 85 No. 1 (2017): 85 (1), 2017 Vol 85, No 2 (2017): Oktober 2017 Vol 85, No 1 (2017): April, 2017 Vol. 84 No. 2 (2016): 84 (2), 2016 Vol. 84 No. 1 (2016): 84 (1), 2016 Vol 84, No 2 (2016): Desember 2016 Vol 84, No 1: Oktober 2016 Vol. 83 No. 2: 83 (2), 2015 Vol. 83 No. 1: 83 (1), 2015 Vol 83, No 2: Desember 2015 Vol 83, No 1: Juni 2015 Vol. 82 No. 2: 82 (2), 2014 Vol. 82 No. 1: 82 (1), 2014 Vol 82, No 2: Desember 2014 Vol 82, No 1: Juni 2014 Vol. 81 No. 2: 81 (2), 2013 Vol. 81 No. 1: 81 (1), 2013 Vol 81, No 2: Desember 2013 Vol 81, No 1: Juni 2013 Vol. 80 No. 2: 80 (2), 2012 Vol. 80 No. 1: 80 (1), 2012 Vol 80, No 2: Desember 2012 Vol 80, No 1: Juni 2012 Vol. 79 No. 2: 79 (2), 2011 Vol. 79 No. 1: 79 (1), 2011 Vol 79, No 2: Desember 2011 Vol 79, No 1: Juni 2011 Vol. 78 No. 2: 78 (2), 2010 Vol. 78 No. 1: 78 (1), 2010 Vol 78, No 2: Desember 2010 Vol 78, No 1: Juni 2010 Vol. 77 No. 2: 77 (2), 2009 Vol. 77 No. 1: 77 (1), 2009 Vol 77, No 2: Desember 2009 Vol 77, No 1: Juni 2009 Vol. 76 No. 2: 76 (2), 2008 Vol. 76 No. 1: 76 (1), 2008 Vol 76, No 2: Desember 2008 Vol 76, No 1: Juni 2008 Vol. 75 No. 2: 75 (2), 2007 Vol. 75 No. 1: 75 (1), 2007 Vol 75, No 2: Desember 2007 Vol 75, No 1: Juni 2007 Vol. 74 No. 2: 74 (2), 2006 Vol. 74 No. 1: 74 (1), 2006 Vol 74, No 2: Desember 2006 Vol 74, No 1: Juni 2006 Vol. 73 No. 2: 73 (2), 2005 Vol. 73 No. 1: 73 (1), 2005 Vol 73, No 2: Desember 2005 Vol 73, No 1: Juni 2005 Vol. 72 No. 2: 72 (2), 2004 Vol. 72 No. 1: 72 (1), 2004 Vol 72, No 2: Desember 2004 Vol 72, No 1: Juni 2004 Vol. 71 No. 2: 71 (2), 2003 Vol. 71 No. 1: 71 (1), 2003 Vol 71, No 2: Desember 2003 Vol 71, No 1: Juni 2003 Vol. 70 No. 2: 70 (2), 2002 Vol. 70 No. 1: 70 (1), 2002 Vol 70, No 2: Desember 2002 Vol 70, No 1: Juni 2002 Vol. 69 No. 2: 69 (2), 2001 Vol. 69 No. 1: 69 (1), 2001 Vol 69, No 2: Desember 2001 Vol 69, No 1: Juni 2001 Vol. 68 No. 2: 68 (2), 2000 Vol. 68 No. 1: 68(1), 2000 Vol 68, No 2: Desember 2000 Vol 68, No 1: Juni 2000 More Issue