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Teknologi Indonesia
ISSN : 01261533     EISSN : 23031913     DOI : -
Core Subject : Science,
JTI is a journal in the Departement of Engineering Sciences - Indonesian Institute of Sciences (LIPI). JTI has policy to publish a new and original research paper or a review paper in The scope of Technology. JTI publishes two issues per year. The journal has been registered with printed-ISSN number as 0126-1533 and electronic ISSN number as 2303-1913.
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Articles 7 Documents
Search results for , issue " Vol 35, No 1 (2012)" : 7 Documents clear
PENAPISAN BAKTERI ENDOFIT PENDEGRADASI PATI DARI TAMAN NASIONAL GUNUNG HALIMUN (TNGH) Melliawati, Ruth; Sulistiyowati, Eki
Teknologi Indonesia Vol 35, No 1 (2012)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jti.v35i1.178

Abstract

In order to produce a good quality biological product such as starch, an enzyme namely amylase was crucially required. Potential microbe could generate sugar through starch degradation. Endophytic bacterias were found on various tissues of plant. Indonesia is really potential to become a source of advantagous endophytic bacteria with its rain forests. The purposes of this research were (1) to screen which endophytic bacteria could degradate starch, (2) to determine the type of reducing sugar generated and (3) to determine the value of amylase activity and theaccumulation of reducing sugar. This research was expected to give some information about the use of endophytic bacteria as an alternative of sugar producer for several industrial sectors. The sceening was practiced to the 30 isolated endophyt bacteria from the plants in Halimun Mountain National Park. Those bacteria was inoculated at a medium named Potato Starch Agar (PSA). The ability to degradate starch could be known when there were clear zones around them. The kinds of sugar were detected by TLC method while the analysis of the amount of reducing sugar and experiment about the activity of amylase were done based on Somogy-Nelson method. The sceening test result that 12 isolates bacteria could grow well on PSA. Three of them generated large clear zones. They wereHL.72 B.153, HL.74 B.156 and HL.89 B.197, with the width of each clear zone were 5,31 cm2, 2,69 cm2 and 6,6 cm2. The experiment of enzymatic activity and the accumulation of of reducing sugar of three choosen isolated bacterias showed that the highest result was HL.89 B.197 with 556,67 Unit and its sugar accumulation value was 631,89 ppm. Then the amylase activitys value of HL.72 B.153 was 505,3 Unit and its sugar accumulation value was 472,29 ppm. The last result was HL.74 B.156 with the amylase activitys value was 263,73 Unit and its sugar accumulation value was 330,21 ppm. The result of TLC showed that a fermentation of HL.72 B.153 was Rf=0,42 of Rf value was same as galactose. HL.74 B.156 Rf value was 0,54 was same as the Rf value of fructose and the Rf value of HL.89 B.197 was 0,54 which was same as the Rf value of glucose.
APLIKASI PENGELOMPOKAN DATA (CLUSTERING OF DATA) DALAM MEMUDAHKAN MONITORING KETERSEDIAAN BAHAN BAKU OBAT Suwarningsih, Wiwin
Teknologi Indonesia Vol 35, No 1 (2012)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (16.051 KB) | DOI: 10.14203/jti.v35i1.174

Abstract

Periodic monitoring of drug raw materials in order to search for expired materials is still done manually. Thus the process of searching for these raw materials is usually slow, since data are store using number based application. Therefore this paper will be discussed how the data for drug raw materials are managed using clustering data mining methods. Drug raw data are stored using clustering so that the searching process will be easier andthe searching time is faster. The result of this research will allow user application to monitor and search for raw materials more easily.
Propionic Acid Metabolism During Anaerobic Biowaste Slurry Digestion Sembiring, Tarzan; Ekoputranto, Agung; Sriwuryandary, Lies; Nilawati, Dewi; Gallert, C; Winter, J
Teknologi Indonesia Vol 35, No 1 (2012)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (16.051 KB) | DOI: 10.14203/jti.v35i1.175

Abstract

Solid waste was found to be a problem in the city sanitary and river management in Indonesia. Bio-waste from traditional market, which is as the major part of the solid waste of the city, is one of the big problem in the sanitary management. To over come of the problem of the bio-waste, the experiment on the anaerobic biodegradation of organic waste slurry in stirred tank bioreactor was executed. The substrate was feeding into reactor and inoculated with an anaerobic enriched mixed culture originally from cow intestinal micro flora. The total solid of substrate was around 10 percent. During the batch experiment showed that the pH value relatively stable around pH 7 but the pH is decreased in the end of the experiment. The degradation of organic slurry is shown by the decreasing of COD content from around 90 g/l to 20 g/l and the formation of volatile fatty acids. Methane content of the biogas produce is more than 60 %. The volatile fatty acids detected are acetic acid, propionic acid, butyric acid and valeric acid. The fluctuation of volatile fatty acids formation and its depletions were relatively good as well as the depletion of Chemical Oxygen Demands, in which the pH value is around 7.5 Propionic acid and butyric acid degrading bacteria were also found in the consortium of mixed culture as indicated by the elimination of those volatile fatty acids during the experiment and in the enrichment cultures. Observation of the cultures of propionic acid degrading enrichment under the microscope shown that the morphology of the bacteria were rod with dimension of 0.81 ?m x 2-3 ?m ( d x l ) and the better gown in pH 6.57.
AKTIVITAS INHIBISI ?-GLUKOSIDASE GRANULAR TEH HIJAU (Camellia sinensis) GRADE Arraca Yabukita HASIL DIAFILTRASI MENGGUNAKAN MEMBRAN NANOFILTRASI Widiyarti, Galuh; Susilowati, Agustine; Aspiyanto, -
Teknologi Indonesia Vol 35, No 1 (2012)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jti.v35i1.176

Abstract

The activity of ?-glucosidase inhibition test of local granular green tea (Camellia sinensis) grade of Arraca Yabukita has been done. Granular green tea obtained by vacuum drying of the concentrate of the diafiltration process using nanofiltration membrane (DF-NF) at 50C for 0-80 hours. Every 8 hours of drying time, the analysis of water content, total polyphenols, and ?-glucosidase inhibitory activity of granular green tea were conducted. The?-glucosidase inhibitory activity test was performed by by Kim Yong-Mu method and compared to quercetin as standard. The results showed that, the drying process increases the ?-glucosidase inhibitory activity of granular green tea. At the optimum drying time 72 hours, the ?-glucosidase inhibitory activity of granular green tea is about 18 times higher than quercetin.
Pengembangan Konten Multimedia Tiga Dimensi Pengolahan Air Kolong Pasca-Penambangan Timah pada Perangkat Telepon Bergerak Andriana, Dian; Nugraheni, Ekasari; Riswantini, Dianadewi; Khotimah, Purnomo Husnul
Teknologi Indonesia Vol 35, No 1 (2012)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jti.v35i1.170

Abstract

Exploitation of tin mining in Province of Bangka Belitung Islands leave traces in the form of pit mines with poor water quality if used for fish farming. Water treatment method under the passive treatment that has been conducted by Limnology researchers in LIPI show pools of water quality improvement. A multimedia content in the form of 3-dimensional animation is made to further disseminate information about the benefits of the use of passive treatment as a medium of learning that can be accessed easily by the fish farmer or local community uses the internet on mobile devices. This paper describes the methodology of development of this multimedia content on mobile devices and includes discussion and testing of accessibility to the public.
KULTUR KOTIL, HIPOKOTIL, DAN EPIKOTIL TANAMAN BUAH NAGA SECARA IN VITRO Sukamto, Lazarus Agus
Teknologi Indonesia Vol 35, No 1 (2012)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (16.051 KB) | DOI: 10.14203/jti.v35i1.177

Abstract

Dragon fruit plant is usually propagated by using seed or stem cutting. Plant propagation by using seed take long time because of slow growth, whereas by using stem cutting could damage mother plant and the resulted plant is limited. Experimental in vitro propagation was carried out by using cotyledon, hypocotyl and epicotyl from seeds grown in vitro. Culture media was modified Murashige and Skoog (MS) formulation that macro and micro-nutrients were subtracted half-full and addition of plant growth regulators (PGR) 6-benzylaminopurine (BA) combined with?-naphthaleneacetic acid (NAA) for cotyledon and hypocotyl, thidiazuron (TDZ) for epicotyl. Cotyledon explants produced the best callus production on media with addition of PGR 3 mg/l BA + 2 mg/l NAA and the best root production on media with addition of 0 mg/l BA + 5 mg/l NAA. Hypocotyl explants produced the best callus production on media with addition of PGR 1 mg/l BA + 4 mg/l NAA or 3 mg/l BA + 2 mg/l NAA and the best root production on control media. Epicotyl explants produced the best callus production on media with addition of PGR 1 mg/l TDZ and the best shoot, shoot height and root production on control media. The highest shoot number production on 0,001 0,01 mg/l TDZ treatment. Epicotyl is the best explant compared to cotyledon and hypocotyl, especially on control in vitro propagation of dragon plant, which could save the expenses without using PGR.
PENGARUH PENAMBAHAN POLY(TETRAFLUOROETHYLENE) PADA KARAKTERISTIK KOMPOSIT BACTERIAL CELLULOSE/ MULTI-WALLED CARBON NANOTUBES Onggo, Holia; Yudianti, Rike
Teknologi Indonesia Vol 35, No 1 (2012)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (16.051 KB) | DOI: 10.14203/jti.v35i1.173

Abstract

In order to obtain new materials as alternative carbon paper, the bacterial cellulose/multi-walled carbon nanotube (BC/MWCNT) composites were made with and without PTFE by helping agarose, via heating process. The effect of PTFE addition on characteristic BC/MWCNT composite have been studied using thermogravimetric analysis (TGA), scanning electron microscopy (SEM) and impedance bridge. The electrically conductivity of BC / MWCNT/ AG composite (without PTFE addition) is 15.1 x 10-2 S/cm (in-plane) and 0.3 x 10-2 S/cm (through plane). Above 150oC, the composite tends to be destroyed leading to electrical conductivity measurements cannot be performed. In the BC/MWCNT/AG/PTFE composite, heating from 150 to 330oC increased electrically conductivityfrom 4.5 to 24.5 x 10-2 S/cm (in-plane) while through-plane electrically conductivity tend to be stable (0.020.03x10-2 S/cm). After heating at 400oC, the composite was seen better thermal stability but the electrically conductivity was decrease because of carbon particles from AG and BC were decomposed and coating of MWCNT. The PTFE addition was not only for increasing the thermal stability but also as coating material of BC/MWCNT composites.

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