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Scripta Biologica
Published by Universitas Jenderal Soedirman
ISSN : -     EISSN : 23553138     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Scripta Biologica is a peer reviewed open access electronic journal published by the Faculty of Biology Jenderal Soedirman University, publishes research papers encompasses all aspects of biology, including; molecular biology, cell biology, and genetics; physiology and reproduction; ecology and conservation; biodiversity, evolution, taxonomy, and biogeography.
Arjuna Subject : -
Articles 2 Documents
 1 
Search results for , issue "Vol 6, No 3 (2019)" : 2 Documents clear
POLLEN STORAGE AND VIABILITY OF TWELVE CITRUS GENOTYPES IN BALI, INDONESIA Ida Ayu Astarini
Scripta Biologica Vol 6, No 3 (2019)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Abstract

The aim of this research was to obtain a method for pollen storage of Citrus species in Bali. Pollen from 12 Citrus genotypes was collected from five different regions; Petang, Plaga, Kintamani, Besakih, and Payangan Village.  Pollen from intact anthers were stored at three different storage temperatures, i.e., 25oC (room temperature), 4oC (refrigerator), and -20oC (freezer), with or without oven dried at 40oC for 20 hours before storage.  Pollen viability was determined using in vitro germination test.  Germination media contained 20 g/l sucrose and 8 g/l agar.  Result shows that -20oC was the best storage temperature.  At this temperature, only slight reduction of pollen viability after 2 months storage.  Storage at room temperature (25oC – 30oC) was not effective; pollen was infected with fungus within a week of storage. Oven dried pre-treatment before storage made better performance on pollen stored at 4oC.  Pollen moisture content decreased after oven dried, which also decreased fungus infection.  Storage of pollen is an important aspect to plant breeding programs, when individuals to be hybridized do not flower at the same time or are geographically separated.  This is the first report on pollen storage and viability of Citrus genotypes grown in Bali. 
POTENSI EKTRAK CACING LAUT Perinereis aibuhitensis SEBAGAI ANTIMIKROBA TERHADAP BAKTERI Staphylococcus aureus dan JAMUR Malassezia furfur Andi Evi Erviani
Scripta Biologica Vol 6, No 3 (2019)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Abstract

Penyakit akibat infeksi mikroba semakin sering terjadi. Penelitian tentang potensi ekstrak cacing laut Perinereis aibuhitensis sebagai antimikroba terhadap bakteri  Staphylococcus aureus dan jamur Malassezia furfur telah dilakukan. Tujuan penelitian ini untuk mengetahui konsentrasi ekstrak cacing laut Perinereis aibuhitensis yang efektif dalam menghambat pertumbuhan bakteri Staphylococcus aureus dan pertumbuhan jamur Malassezia furfur. Hasil penelitian yang diperoleh menunjukkan bahwa ekstrak cacing laut yang menggunakan pelarut etanol 96 % dan diperlakukan pada kultur bakteri Staphylococcus aureus dengan konsentrasi ekstrak cacing laut 7.5%, 15%, 30% dan 60% memperlihatkan terbentuknya zona hambat pada media dengan lama inkubasi 1x24 jam dan 2x24 jam, dan memperlihatkan zona hambat terbesar pada konsentrasi ekstrak 60%. Demikian pula pada Jamur Malassezia furfur, terbentuk zona hambat pada konsentrasi ekstrak 7,5%;15%;30% dan 60%. Zona hambat terbesar terlihat pada konsentrasi ekstrak 60%. Dari hasil penelitian yang telah dilakukan dapat disimpulkan bahwa ekstrak cacing laut Perinereis aibuhitensis berpotensi sebagai antimikroba terhadap bakteri Staphylococcus aureus dan jamur Malassezia furfur  .Key Words: Perinereis aibuhitensis, antimikroba, Staphylococcus aureus, Malassezia furfur

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