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Scripta Biologica
Published by Universitas Jenderal Soedirman
ISSN : -     EISSN : 23553138     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Scripta Biologica is a peer reviewed open access electronic journal published by the Faculty of Biology Jenderal Soedirman University, publishes research papers encompasses all aspects of biology, including; molecular biology, cell biology, and genetics; physiology and reproduction; ecology and conservation; biodiversity, evolution, taxonomy, and biogeography.
Arjuna Subject : -
Articles 3 Documents
 1 
Search results for , issue "Vol 6, No 4 (2019)" : 3 Documents clear
ANALISIS IN SILICO PERANCANGAN crRNA CRISPR DENGAN TARGET SEKUENS PENYANDI SQUALENE SYNTHASE PADA Artemisia annua Linn. Ivan Tjahja Pranata
Scripta Biologica Vol 6, No 4 (2019)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2019.6.4.1105

Abstract

Artemisinin adalah salah satu jenis fitofarmaka yang penting oleh karena beberapa potensi medisnya. Meskipun demikian, produktivitas Artemisia annua di Indonesia secara umum masih berkisar di bawah 0,5% sehingga secara komersial belum layak untuk dibudidayakan. Salah satu faktor utama yang menghambat produktivitas artemisinin adalah keberadaan gen SQS yang berkompetisi dengan gen ADS dalam penggunaan susbtrat farnesil difosfat. Kompetisi ini menurunkan kadar prekursor kunci bagi pembentukan senyawa artemisinin. Teknik penyuntingan genom CRISPR berbasis non-GMO dapat diaplikasikan untuk mengatasi hambatan ini. Mekanisme knockout yang disebabkan oleh sistem CRISPR pada gen SQS dapat menekan tingkat kompetisi substrat yang terjadi pada jalur biosintesis artemisinin sehingga diharapkan meningkatkan kadar artemisinin. Sebelum dilakukan transformasi secara in vitro, perlu dilakukan analisis in silico untuk pembuatan model crRNA sintetis. Dalam penelitian ini, digunakan 2 jenis perangkat lunak berbasis bioinformatika yaitu E-CRISP dan CRISPRdirect untuk pelacakan on-target dan off-target. Berdasarkan hasil perancangan yang telah dilakukan, 10 desain crRNA sintetis berhasil dibentuk dengan model crRNA_F5 sebagai desain yang paling optimal. Model crRNA_F5 ini memiliki sekuens protospacer 5’- GGTGTAGTCAAATTGAGACG-3’ dan sekuens PAM 5’-TGG-3’. Pemilihan desain crRNA_F5 dipertimbangkan dari nilai efficacy sebesar 78.57, skor Doench dan skor Xu tertinggi, serta minimalisasi jumlah off-target pada sekuens 8mer pada jumlah 259. Model ini sama sekali tidak memiliki off-target pada sekuens 20mer dan 12mer.
GROWTH OF SEAWEED Gracilaria verrucosa CULTURED ON DIFFERENT INITIAL WEIGHT WITH LONGLINE METHODS IN KARIMUNJAWA WATERS TITIK SUSILOWATI; DICKY HARWANTO; CHONDRORESMI BANOR FAWWAZ; ALFABETIAN HARJUNO CONDRO HADITOMO; SARJITO SARJITO
Scripta Biologica Vol 6, No 4 (2019)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1019.551 KB) | DOI: 10.20884/1.sb.2018.5.4.1120

Abstract

One factor that can optimize seaweed production is determining the initial weight of planting. However, planting seaweed in open waters is very vulnerable to aggregation from herbivorous fish. This study aims to determine the best initial weight for the growth of G. verrucosa, which is cultivated in net cage by the longline method. Net cages, made of nylon, are applied as protection for G. verrucosa from aggregation of herbivorous fish. This research was conducted in Karimunjawa waters, Jepara Regency, Central Java, for 42 days. The experimental design used was Randomized Block Design (RBD), with three treatments and four replications each. The treatment was different initial planting weights of 25, 50, and 75 g. The observed variables included culture techniques, relative growth rates, specific growth rates, and water quality. The analysis showed that the different initial weight was very significant (P <0.01) on the growth of G. verrucosa. Treatment with an initial weight of 25 g gave the best relative growth rate (2.07±0.25%.d-1), and the best specific growth rate (1.48±0.13%.d-1). Furthermore, this study was able to prove that the use of a net planting cage on a seaweed hanger can avoid aggregation of herbivorous fish, which is indicated by the growth of G. verrucosa.
EFEK TERAPI PISANG TONGKA LANGIT (Musa troglodytarum) TERHADAP INDEKS ERITROSIT MENCIT (Mus musculus) MODEL MALARIA Efraim Samson
Scripta Biologica Vol 6, No 4 (2019)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2019.6.4.1114

Abstract

Malaria is an infectious disease that is still a major health problem in various countries, including Indonesia. Malaria parasite infection can induce changes in structure, physiology and biochemistry in hematology causing damage to erythrocytes. The use of plants in malaria treatment (phytotherapy) began to be developed at this time. Tongka langit banana (Musa troglodytarum) is one type of banana that has the potential to be developed. This study aimed to see the therapeutic effect of tongka langit banana against the erythrocyte index of mice with malaria models. The results showed that the therapy of tongka langit banana was able to increase the erythrocyte index (RBC, MCV, MCH, and MCHC) in mice with malaria, and the most effective dose is 0.75 gr/mice/day.Key Words: Tongka Langit Banana, Erythrocyte Index, Phytotherapy, Malaria.

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