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Induksi Ketahanan Tanaman Kedelai (Glycine max (L.) Merril) dengan Cendawan Endofit Trichoderma harzianum dan Beauveria bassiana untuk Menekan Penyakit Busuk Pangkal Batang (Sclerotium rolfsii) Wildatun Munawara; Nanang Tri Haryadi
Jurnal Pengendalian Hayati Vol 3 No 1 (2020)
Publisher : Fakultas Pertanian Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jph.v3i1.17146

Sclerotium rolfsii is a pathogen that causes stem rot disease that causes a decrease in soybean production. This stem rot disease can cause damage to all parts of the plant both in the vegetative and generative phases. Proper control is needed to reduce stem rot disease by induction of plant resistance using endophytic fungi such as Trichoderma harzianum and Beauveria bassiana. This experiment used a Completely Randomized Design (CRD) with 4 treatments that were repeated 5 times, namely P1 = control, P2 = T. harzianum application + S. rolfsii inoculation, P3 = B. bassiana application + S. rolfsii inoculation, and P4 = Combined application (T. harzianum + B. Bassiana) + S. rolfsii inoculation. Based on research that has been done shows that the incubation period and phenol analysis in soybean plants did not significantly affect soybean crop resistance while in the incidence of disease and disease severity showed a very significant effect on soybean crop resistance. In this study the plants that were applied with T. harzianum and B. bassiana were included in the category of resistant to S. rolfsii pathogens, while the control treatment was included in the susceptible category to S. rolfsii pathogens.

Aplikasi Herbisida Berbahan Aktif Campuran Atrazin-Mesotrion dan Paraquat dalam Pengendalian Gulma pada Pertanaman Jagung (Zea mays L.) Rima Esa Lolitasari; Saifuddin Hasjim
Jurnal Pengendalian Hayati Vol 2 No 1 (2019)
Publisher : Fakultas Pertanian Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jph.v2i1.17138

Corn in cultivation is inseparable from several other organism disorder s that can inhibit or reduce that productivity. One of the disorders is caused by weeds. Application of herbicides with active ingredients on the mixture of atrazine-mesotrion, and paraquat which is applied to corn plants has its own effect on corn plants if it is applied not according to the time or recommended dosage. The experimental design used, namely Randomized Completely Block Design (RCBD) with 6 treatments and 4 replications, namely P0 (control or uncontrolled), P1 (manual weeding 21 hst and 42 hst), P2 (Application of atrazine a nd mesotrione dose of 1.5 l / ha 21 hst), P3 (Application of paraquat dose 1 l / ha 21 hst), P4 (Application of atrazine and mesotrione dosage 1.5 l / ha 21 hst and 42 hst), P5 (Application of paraquat dose 1 l / ha 21 hst and 42 hst). The results showed that the use of herbicides with active ingredients atrazine, mesotrione, and paraquat had been able to effectively reduce the biomass of broad-leaf weeds and grasses. The use of the paraquat active ingredient applied at 21 hst showed the highest phytotoxicity symptoms in weeds and in maize plants. The use of two active ingredients of herbicide, manual weeding and control had no effect on plant height, growth rate, cob length, ear circumference, and had effect on shell weight.

Pengaruh Pemberian Vermikompos pada Media Tanam Terhadap Efektivitas Gliocladium sp. dalam Mengendalikan Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Semangka (Citrulus vulgaris, Schard) Winda Ruliyanti; Abdul Majid
Jurnal Pengendalian Hayati Vol 3 No 1 (2020)
Publisher : Fakultas Pertanian Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jph.v3i1.17147

Watermelon is one of the plants that have good prospects and has high economic value, but its production is currently experiencing a decline. Decreased production caused by the pathogen Fusarium oxysporum which causes fusarium wilt disease in plants. losses caused by fusarium wilt attacks reach 20-30%. Utilization of Gliocladium sp. and vermicompost is an alternative step in controlling fusarium wilt. The results showed that the use of Gliocladium sp. and vermicompost can suppress the development of fusarium wilt disease. The use of Gliocladium sp. and vermicompost 250 grams/polybag can suppress the attack of fusarium wilt disease with a percentage of suppression of the incidence of disease by 70% and suppress the severity of the disease by 38.5% compared to controls. and has a control effectiveness value of 73.68% which is categorized very well. The use of Gliocladium sp. and vermicompost also affect plant growth and production.

Pemanfaatan Deleterious rhizobacteria Untuk Mengendalikan Gulma Utama Tanaman Padi di Kabupaten Jember Yogi Ardhi Cahyadi; Saifuddin Hasjim
Jurnal Pengendalian Hayati Vol 2 No 2 (2019)
Publisher : Fakultas Pertanian Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jph.v2i2.17139

The purpose of the study was to determine the characteristics of plant pathogens from Deleterious rhizobacteria (DRB) that have the potential as biological control agents in the main weeds of rice plants. This research was conducted in April-August 2019 at the Plant Protection Laboratory, Faculty of Agriculture, University of Jember. Rhizobacteria sampling was taken from rice fields in Mayang District. Weed sampling technique is done by purposive randomized sampling. The collections of pathogen-infected weeds are then isolated using laminar airflow and identified through several test stages. DRB exploration results obtained by two microorganisms that can be used as biological control to control the main weeds of rice plants from the Pseudomonas group, they are P. syringae pv. glycinea (M1) and P. syringae pv. syringae (M2). Based on the identification of the two isolates morphologically that (M1) has a greenish-white color with a flat edge type. Whereas the isolate code (M2) has a yellowish murky white color with a rather irregular jagged edge type. However, the two isolates have similarities in spherical shape. The physiological observations of both M1 and M2 isolate from the Catalase test, fluorescent pigments produce gram-positive (+) while gram tests produce gram-negative (-). The conclusion of this research is the characterization of P. syringae pv. glycinea (M1) and P. syringae pv. syringae (M2) both morphologically and physiologically.

Potensi Bacillus spp. sebagai Agen Biokontrol untuk Menekan Layu Fusarium (Fusarium oxysporum) pada Tanaman Melon (Cucumis melo L.) Setiadi Jitendhriyawan Suwarno; Rachmi Masnilah
Jurnal Pengendalian Hayati Vol 3 No 1 (2020)
Publisher : Fakultas Pertanian Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jph.v3i1.17148

Fusarium wilt caused by F. oxysporum, is a disease that often attacks melon plants. F. oxyporum is a fungus that infects through the roots and clogs vascular vessels in plants and causes plants to wither with necrotic symptoms. Bacillus spp as a biological agent capable of controlling fusarium wilt by antibiotic mechanism. Bacillus spp obtained was then carried out by testing to determine its potential as a biological recognition agent. This research was carried out starting from the isolation of F. oxysporum, isolation and inoculation of Bacillus spp., Gram test, hypersensitivity test using tobacco plants, to calculate the intensity of attacks and analyze. The study was conducted with 5 treatments namely control, F. oxysporum without Bacillus spp., F. oxysporum with isolates BJM4, BJM5, and BJM9. The results showed that BJM5 isolates can suppress fusarium wilt disease with a disease severity value of 23.75%, the lowest compared to all treatments applying Bacillus spp. this was also shown by the results of antagonistic tests on PDA media that BJM5 isolates could suppress F. oxysporum fungi by 0.6 or 60%.

Uji Efektivitas Konsentrasi Spodoptera litura – Nuclear Polyhedrosis Virus (SlNPV) JTM 97C Formulasi Bubuk Terhadap Larva Spodoptera litura Fabricius (Lepidoptera: Noctuidae) Pada Tanaman Kedelai M Novel Ghufron Syahroni; Nanang Tri Haryadi
Jurnal Pengendalian Hayati Vol 2 No 2 (2019)
Publisher : Fakultas Pertanian Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jph.v2i2.17140

Spodoptera litura (armyworm) is an important leaf pest that is polyphagic and has a broad range of hosts. Spodoptera litura is the main pest in soybean plants and can cause up to 50% damage and often results in decreased productivity and even crop failure. Spodoptera litura is widespread in areas with hot and humid climates from the subtropics to the tropics. S. litura pests attack cultivation plants in the vegetative and generative phases. The prospect of insect pathogens as a substitute for chemical insecticides is quite good in terms of their effectiveness and impact on the environment. Nuclear Polyhedrosis Virus (NPV) is one of the Baculoviridae viruses that infect the larval stage. Spodoptera litura Nuclear Polyhedrosis Virus (SlNPV) is a pathogenic virus that infects armyworms. SlNPV JTM 97C is an effective isolate in controlling larvae of Spodoptera litura. The larvae used in this study were larval instar 4, instar 5, and instar 6. The study was conducted using a Completely Randomized Design (CRD) with 6 treatments and 3 replications. The treatments consisted of controls, 1 g / l, 2g / l, 3 g / l, 4 g / l, and 5 g / l. Each treatment was applied to instar 4, instar 5 and 6 instar larvae. The results showed that SlNPV JTM 97C affected mortality, stopped eating and pupa formation of instar 4, instar 5 larvae, but did not affect instar 6 larvae. The younger the larval instar the higher the pathogenicity response.

Viabilitas Bacillus sp. Sebagai Agen Antagonis Patogen Tanaman Dalam Formulasi Berbahan Dasar Tepung Dhirham Khusma Fakhruddin; Suhartiningsih Dwi Nurcahyanti
Jurnal Pengendalian Hayati Vol 3 No 1 (2020)
Publisher : Fakultas Pertanian Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jph.v3i1.17151

Bacterial biopesticide formulations needed because the use of bacteria in the suspensions can reduce the ability to control disease in plants. Therefore, a bacterial suspension needs to be mobilized in the formula with a carrier (Carrier) to maintain the viability of bacteria. This research uses the formulation of Bacillus sp. made from rice flour, corn, tapioca and talc with the addition of urea, glucose and CMC. Formulation of Bacillus sp. flour based with the addition of urea, glucose and CMC were tested with Xanthomonas axonopodis pv. glycines and Colletotrichum sp. in vitro. The results showed that Bacillus sp. after formulation it is still able to maintain viabiitas and antagonistic power. Viability of Bacillus sp. the best is in the formulation of rice flour at 14 hsi at 4.94 X 1014 cfu / g while the inhibition zone of Bacillus sp. the best is the corn flour formulation of 13.1 mm and the inhibitory capacity of Bacillus sp. the best is in tapioca flour formulation at 42 hsi at 62.89%

Pengendalian Penyakit Pustul Xanthomonas axonopodis pv. glycines Pada Kedelai Dengan Bacillus spp. Asal Filosfer Gulma di Pertanaman Kedelai Siti Rahayu; Suhartiningsih Dwi Nurcahyanti
Jurnal Pengendalian Hayati Vol 2 No 2 (2019)
Publisher : Fakultas Pertanian Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jph.v2i2.17141

Soybean pustule disease by Xanthomonas axonopodis pv. glycines (Xag) is one of the important diseases of soybean plant. Application Bacillus spp. origin of weed phyllosphere has a great potential as an alternative to control because it is isolated to origin of same region as Xag is the phyllosphere. Research by Nurcahyanti and Ayu obtained the best five isolates of Bacillus spp. of weeds phyllosphere in soybean cropping in inhibiting Xag in vitro. This study used the complete random draft (RAL) 6 treatment, 4 repeats, each unit consists of 4 plants wich treatment application of 5 isolates of Bacillus spp. namely K = control; A = Bacillus JG 1.3; B = Bacillus JG 3.6; C = Bacillus JG 1.4.1; D = Bacillus BGd 1.1; E = Bacillus Bp 2.2. The results showed that application of Bacillus spp. could inhibit Xag in vitro with bacteriostatic mechanism and isolates Bacillus BGd 1.1 has the greatest inhibition of 14.25 mm. Fifth Bacillus spp. can suppress the development of disease and isolates Bacillus BGd 1.1 has best results with the incubation period during 13 HSI, the severity of disease 10.07%, infection rate 0.045 unit/day as well as the effectiveness of control 85.72%. The fifth isolates of Bacillus spp. can not increase the number of leaves but can increase number of branches and isolates Bacillus JG 1.3, Bacillus JG 1.4.1 and Bacillus BGd 1.1 can increase the height of soybean crop.

Pengaruh Penggunaan Beberapa Varietas dan Aplikasi Pseudomonas fluorescens untuk Mengendalikan Penyakit Bulai (Peronosclerospora maydis) pada Tanaman Jagung (Zea mays L.) Muhamad Aditia Ulhaq; Rachmi Masnilah
Jurnal Pengendalian Hayati Vol 2 No 1 (2019)
Publisher : Fakultas Pertanian Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jph.v2i1.17131

Corn is one of the commodities that have high economic value and important role in meeting food needs in Indonesia. Unfavorable conditions on the rate of the higher demand for corn. Pests to be one limiting factor that causes a decrease in the production of corn. Peronosclerospora maydis is a pathogen that causes downy mildew on corn. P. maydis infects corn plants at the age of 2-3 weeks, with the level of damage reaches 80-100%. Control efforts against this disease one of them using antagonistic microbes such as bacteria Pseudomonas fluorescens. P. fluorescens has the potential to control downy mildew because it produces compounds that are antibiosis as chitinase enzymes that can hydrolyze the cell walls of fungi. The purpose of this study to determine the effect of the interaction of P. fluorescens isolates applications and the use of some varieties to suppress downy mildew attack P. maydis on corn. The method used is to use a random test design of a factorial group with 2 factors. The first factor is the type of varieties with three levels namely V1: Pioneer 27, V2: Pioneer 21 and V3: Bonanza. The second factor is a type of isolates P. fluorescens with three levels namely P1: without the application of P. fluorescens, P2: Isolates P. fluorescens (A) and P3: Isolate of P. fluorescens (B). The result is the application of P. fluorescens and use of some varieties can suppress downy mildew P. maydis.

Manipulasi Mikrohabitat Dengan Sistem Tanam Polikultur Sebagai Stabilizer Ekosistem Untuk Pengelolaan Hama dan Musuh Alami Pada Tanaman Bawang Merah (Allium ascalonicum Linn.) Rizal Sukarno; Sigit Prastowo
Jurnal Pengendalian Hayati Vol 2 No 2 (2019)
Publisher : Fakultas Pertanian Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jph.v2i2.17142

Shallot (Allium ascalonicum Linn.) constitutes one of the commodities that has important meaning for Indonesian people with the limiting factor in the production is the pest attack. One of these pest control techniques is by controlling technical culture of polyculture planting system. This research was conducted in July to October 2019 which was located in Banyuputih Village, Wringin District, Bondowoso Regency. This study used a Randomized Block Design (RBD) with six treatment, namely: P1 = polyculture of shallot + Lemongrass; P2 = polyculture of shallot + celery; P3 = Polyculture of shallot + mustard; P4 = Polyculture of shallot + Lemongrass + mustard; P5 = Polyculture of shallot + celery + mustard; P6 = Monoculture of shallot. Each treatment was repeated 4 times. Observation started at 30 days after planting. Data collection was done by observing directly on the sample plants. Sampling was carried out using Yellow trap and pit fall trap. Samples were taken at each plot 10 times with intervals of 4 days. Observations included collecting the pest insects and natural enemies that were found, counting the number of populations in each species, scoring towards the damage plants. The results showed that shallot planting by polyculture with different types of plants affected the population of pests and natural enemies as well as the level of diversity of insects in shallots. Planting shallots by polyculture has been proven to control pest populations compared to planting shallots by monoculture. Polyculture planting with two types of plants proved to be better than polyculture planting with three types of plants where the best treatment was found in P1, namely polyculture of shallot and lemongrass with the smallest pest population which was 17.5.

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Jawa timur

INDONESIA