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INDONESIA
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Published by Perhimpunan Dokter Spesialis Patologi Klinik Indonesia
ISSN : 08544263     EISSN : 24774685     DOI : https://dx.doi.org/10.24293
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) is a journal published by “Association of Clinical Pathologist” professional association. This journal displays articles in the Clinical Pathology and Medical Laboratory scope. Clinical Pathology has a couple of subdivisions, namely: Clinical Chemistry, Hematology, Immunology and Serology, Microbiology and Infectious Disease, Hepatology, Cardiovascular, Endocrinology, Blood Transfusion, Nephrology, and Molecular Biology. Scientific articles of these topics, mainly emphasize on the laboratory examinations, pathophysiology, and pathogenesis in a disease.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 12 Documents
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Search results for , issue "Vol 17, No 2 (2011)" : 12 Documents clear
DIAGNOSIS JANGKITAN (INFEKSI) VIRUS DENGUE DENGAN UJI CEPAT (RAPID TEST) IgA ANTI-DENGUE Sri Kartika Sari; Aryati Aryati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 2 (2011)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i2.1020

Abstract

Dengue IgM, IgG Capture ELISAs and NS1 Ag ELISA become the most widely used serological methods for dengue diagnosis untilnow. Previous studies reported a possible use of IgA antibodies for dengue virus as a new serologic marker to make dengue infectionactive. In the present study, the performance of IgA anti-dengue rapid test as a new marker of dengue infection was assessed. In thisstudy, the sera were obtained from 30 dengue virus infection patients and 30 non dengue virus infection patients. Thirty dengue pairedsera were collected twice, at the time of hospital admission (acute) and at discharge (convalescent). All sera samples were characterizedusing dengue reference ELISAs (NS1 Ag, Dengue IgM and IgG capture ELISAs). The results of IgA anti-dengue rapid test were comparedwith the corresponding dengue reference tests. The sensitivity and specificity of IgA anti-dengue rapid test respectively were 78.3% (95%CI: 65.5–87.5%), and 73.3% (95% CI: 55,6–85,8%). Meanwhile, from acute sera, sensitivity of IgA anti-dengue rapid test was 83.3%(95% CI: 64.5–93.7), higher than IgM (73.3%, 95% CI: 53.8–87.0), IgG (66.7%, 95% CI: 47.1–82.1) and NS1 Ag ELISAs (60%,95% CI: 40.7–76.8). Positive IgA anti-dengue rapid test results in acute sera was higher in the secondary (91%) than primary infection(57%). IgA anti-dengue rapid test can be considered as a new marker for dengue infection, because it gives a high sensitivity, especiallyin the acute phase and in the secondary infections as well.
LEUKOSITOSIS BER-FLAGGING BINTANG () BERPOTENSI ADANYA INTERFERENSI ALAT ANALISIS HEMATOLOGI OTOMATIS Christine Sugiarto; Leni Lismayanti; Nadjwa Zamalek Dalimoenthe
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 2 (2011)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i2.1026

Abstract

Leukocytosis is a condition in which there is an increasing number of white blood cell count in the peripheral blood compared to thenormal range based on age. Several conditions can amplify leukocyte count from haematological auto analyzers, not only those whichcorrespond to the pathologic and physiologic condition, but also with other factors, such as diluent and haematological auto analyzer’smethods. The information about these factors should be evaluated to lessen errors in the patient’s diagnosis and therapy. This casereport describes a leukocytosis in a 35-day old baby boy, diagnosed as duodenal obstruction, admitted in Paediatric Surgery Department,Hasan Sadikin Hospital, Bandung. Discrepancies occurred in this patient’s leukocyte count with some different haematological autoanalyzers. The leukocyte count from the auto analyzer by impedance method and ammonium salt diluent was 129.200/mm3 which wasindicated by a star-flagged (), while from the auto analyzer with light scatter method and anhydrous sodium sulphate and sodiumchloride diluent was 9.200/mm3, from manual count by the counting chamber with Turk diluent was 14.200/mm3 and the estimationby peripheral blood smear was 7.000–10.000/mm3. False leukocytosis by auto analyzer with impedance method was caused by thelimitation of the analyzer’s method and by the erythrocyte lysine reagent (diluent) using ammonium salt. As investigated in this case,the interferences were thought as being caused by the Lyses-resistant Red Blood Cells, thus the non-lysed/lyses cells which were enlargedin size were identified as leukocytes other than erythrocytes. It can be that the white blood concluded cell count examination which isindicated by star-flagged (), or white blood cell count >100.000/mm3 must be confirmed by manual examination (counting chamberand peripheral blood smear) or by another haematological auto analyzer method that has a different and more potent diluent

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