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Journal of Stem Cell Research and Tissue Engineering
Published by Universitas Airlangga
ISSN : 26141264     EISSN : 26141256     DOI : https://dx.doi.org/10.20473/jscrte
Core Subject : Health, Science, Engineering,
Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary
Journal of Stem Cell Research and Tissue Engineering (JSCRTE) is published by Stem Cell Research and Development Center, Airlangga University. Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancerstem cells, developmental studies, genomics and translational research. Special focus of JSCRTE is on mechanisms of pluripotency and description of newly generated pluripotent stem cell lines. Articles that go through the selection process will be review by peer reviewer or editor. The journal is published regularly twice a year in December and May. Every publication consists of 60-70 pages and 5 scientific articles in the form of research, study literature, and the case study in English. The contributors Journal of Stem Cell Research and Tissue Engineering are Stem Cell researchers, lecturers, student and practitioners that came from Indonesia and abroad.
Arjuna Subject : Kedokteran - Imonologi dan Alergi
Articles 90 Documents
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The Effect Of Mangrove Leaf Extract Dosage Sonneratia Alba On Hela Cell Viability Fitria Devi Suryaningrum
Journal of Stem Cell Research and Tissue Engineering Vol. 5 No. 1 (2021): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v5i1.29383

Abstract

Cervical cancer is caused due to infection from the Human Papilloma Virus (HPV) which attacks the sexually active female reproductive organs. Treatment is carried out alternatively using natural ingredients such as mangroves. Sonneratia alba is a type of mangrove plant that has been used in alternative medicine because of its potential as an anticancer. This study aims to determine the effect of Sonneratia alba mangrove extract on heLa cell viability. The Sonneratia alba leaf powder was extracted using stratified maceration. The solvents used include n-hexane, ethyl acetate, and ethanol. The results showed that the LC50 value was 3.59 ppm, this means that the ethyl acetate extract has toxic properties. Phytochemical test results of Sonneratia alba leaf extract contain alkaloid compounds, steroids / triterpenoids, and tannins. The results of the test yield extract were 12.60%, extract water content was 21.24%, and total phenol was 504.08 mg / g GAE Test The results of the LC-MS test resulted in the suspicion of compounds including diosmetin, caffeine, and turmeron. The ethyl acetate extract of Sonneratia alba leaves was cytotoxic against heLa cell viability with the resulting IC50 value of 478.630 µg / mL
The Dose Effect of Mangrove Leaf Extract (Rhizophora apiculata) on Anticancer Activity in HeLa Cells Dwi Mahfud Maulana
Journal of Stem Cell Research and Tissue Engineering Vol. 5 No. 1 (2021): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v5i1.29380

Abstract

Disease cancer caused by abnormal growth of tissue where there has been an error, fast and out of control. Judging from the fact of gender, more than 270,000 women die every year caused by cervical cancer. To inhibit the growth of cancer cells, a compound is needed that causes the cell cycle to stop so that the ability of cell proliferation decreases. Alkaloid compounds can inhibit proliferation through oxidative inhibition processes that can cause cancer. Mangrove plants have potential as anticancer, antimicrobial, and antioxidant. The content of chemical compounds found in mangroves are flavonoids, steroids, alkaloids, phenolites, saponins and tannins. These compounds show high antioxidant activity and are shown to have a real relationship with the properties of the material's bioactivity against cancer cells. One of the mangrove species is Rhizophora apiculata. The purpose of this study was to determine the IC50 value produced by Rhizophora apiculata mangrove leaf extract on HeLa cell viability and to see the effect of Rhizophora apiculata mangrove leaf extract dosage on HeLa cell viability. The method used in this research is the experimental method. The research parameters included yield, proximate test, phytochemical test, toxicity test, total phenol test, cytotoxicity test and LC-MS test. The experimental design used was a simple and complex completely randomized design (CRD) with the Tukey test.The results of this study showed that the highest yield was in the ethanol extract of 5.91%, while the n-hexane and ethyl acetate extracts respectively had yields of 1.18% and 1.31%. The results of the proximate test on the water content of leaves and powder were 64.53% and 13.86%, respectively, the results of the ash content in the leaves and powder of Rhizophora apiculata were 3.94% and 8.41%, respectively. while the water content in the extract obtained the highest yield in the ethanol extract of 21.42%, while the n-hexane extract and ethyl acetate extract were 11.08% and 15.42%, respectively. For phytochemical results, it was found that n-hexane extract only contained alkaloids, flavonoids and steroids. Ethyl acetate extract contains steroid compounds. Meanwhile, the ethanol extract contains the most bioactive compounds, namely saponins, flavonoids, tannins and triterpenoids. The toxicity test using the Brine Shrimp Lethality Test (BSLT) method resulted in the lowest IC50 of ethanol extract at 49.45 ppm while the n-hexane and ethyl acetate extracts were 251.63 ppm and 920.45 ppm respectively. In the total phenol test, the n-hexane extract was 66.79 mg GAE / 100 gr, 222.97 mg GAE / 100 gr ethyl acetate extract and 929.04 mg GAE / 100 gr ethanol extract. HeLa cell cytotoxicity testing using the MTT method (3- (4,5-dimethiltiazol-2-yl) -2,5-dipheniltetra zolium bromide) assay resulted in the highest cell viability value at a dose of 125 ppm of 46.97%. As for the doses of 250 ppm, 500 ppm 1000 ppm, and 2000 ppm resulted in a percentage of viability of 42.95% 37.70% 35.82% and 32.12%, respectively. The IC50 value of Rhizophora apiculata leaf extract was 64.42 ppm. This value indicates that the Rhizophora apiculata extract is toxic to HeLa cells.
Effect Of Alpha-Tocopherol On Spermatozoa Death Of Apoptosis and Necrosis in Rats (Rattus Norvegicus) Exposed 2,3,7,8- Tetrachlorodibenzo-P-Dioxin Valderama Gomang
Journal of Stem Cell Research and Tissue Engineering Vol. 5 No. 1 (2021): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v5i1.29381

Abstract

The aims of this research was to investigate effect of alpha-tocopherol on spermatozoa death in form of apoptosis and necrosis in rats (Rattus norvegicus) exposed 2,3,7,8- tetrachlorodibenzo-p-dioxin. Male rats were administered TCDD and alpha-tocopherol in experimental groups. Five experimental groups of a combination of TCDD and alpha- tocopherol were designed as follows; 0.5 ml of corn oil (control negative group, K-), 700 ng/kg/d of TCDD and 0.5 ml of corn oil (treatment control), 700 ng/kg/d of TCDD and 77 ng/kg/d of alpha-tocopherol (Group P1), 700 ng/kg/d of TCDD and 140 mg/kg/d of alpha-tocopherol (Group P2), 700 ng/kg/d of TCDD and 259 mg/kg/d of alpha- tocopherol (Group P3) respectively. Alpha-tocopherol and TCDD were given by oral gavage for 20 days. The result indicated that TCDD increased spermatozoa death in form of apoptosis and also necrosis. Alpha-tocopherol at 259 mg/kg/d most effective to decreased spermatozoa death number. The conclusion indicated that alpha-tocopherol at 259 mg/kg/d effective to decreased the spermatozoa death in form of apoptosis and necrosis in rats (Rattus norvegicus) exposed 2,3,7,8-tetrachlorodibenzo-p-dioxin.
Effect Of Mangrove Leaf Extract Dosage Rhizophora Mucronata Lmk. On The Viability Of Hela Cells Rohadatul Alsy
Journal of Stem Cell Research and Tissue Engineering Vol. 5 No. 1 (2021): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v5i1.29382

Abstract

This study aims to determine the IC50 value of the mangrove leaf extract type Rhizophora mucronata Lmk. against the viability of Hela cells. The samples extracted with three types of solvents were previously conducted preliminary research on phytochemical compounds and their toxicity values with the Meted Brine Shrimp Letality Test (BSLT). In the toxicity test, the highest level of toxicity was obtained in the ethanol extract with a value of 166.72± 7.72 ppm, then the sample was continued for the cytotoxicity test using the MTT method. The dosage variants used in this study were 62.5 ppm; 125 ppm; 250 ppm; 500 ppm; and 1000 ppm. The variation in dosage shows an effect on the viability of Hela cells, namely a decrease in the percentage of living cells along with the addition of the ethanol extract dose of Rhizophora mucronata Lmk leaves. which is given. And the IC50 value obtained from this study was 63.67 µg / mL with the toxic category
Difference Between Bone Healing Process with The Use Of Demineralized Freeze Dried Bone Xenograft and Bovine Bone Hydroxyapatite Xenograft Materials Zefry Zainal Abidin
Journal of Stem Cell Research and Tissue Engineering Vol. 5 No. 2 (2021): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v5i2.33149

Abstract

Autogenous bone graft is the gold standard for bone defects treatment, however due to their limitation and the donor site morbidity may caused many surgeons use a xenograft type of bone grafting to cope the problem. Demineralized Freeze Dried Bone Xenograft (DFDBBX) which contains of growth factors, have a good biocompatibility. The aim of this study is observed the difference in bone healing processes between DFDBBX and Bovine Bone Hydroxyapatite Xenograft (BBHAX). Bicortical bone defects were created in the mandibular corpus of 30 New Zealand White Rabbits. The groups were divided into 3 groups which the first group were treated with DFDBBX into the hole and the negative control group was left perforated. The other group was treated with BBHAX. All group were evaluated after second and fourth weeks to count the ammount of osteoblast, osteoclast cells, Collagen-1 (Coll-1) and alkalin phosphatase (ALP). The second week of observation showed a significant difference of mean 12,45, SD 2,97 (p<0,05) in osteoblast cells. In Coll-1 showed with mean 13,2 SD 2,68 (p<0,05). The result of ALP showed with mean 14,6 SD 2,70 (p<0,05). In the the fourth week observation showed increased of osteoclast cells with mean 7,043, SD 2,77 (p< 0,05) and for Coll-1 with mean 17,6, SD 2,30 (p< 0,05). DFDBBX showed more effective in treating bone defects of mandible of new zealand white rabbits in second week of observation.
Viability Assay Of Human Fibroblast Cells Treated by Water Hyacinth Leaf Extract After 24 Hours Incubation Ully Nafisah Wardi
Journal of Stem Cell Research and Tissue Engineering Vol. 5 No. 2 (2021): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v5i2.33147

Abstract

Inflammation and alveolar bone resorption are indications of periodontal disease, which is a chronic inflammatory illness caused by bacterial colonization that damages the soft and hard structures that support the teeth. In response to persistent tissue injury and chronic inflammation, fibroblasts also play a role in the synthesis and maintenance of extracellular matrix, cell proliferation, and cell differentiation. Fibroblasts play a crucial part in the healing of wounds. Phenols, alkaloids, flavonoids, and tannins are some of the health-promoting components found in water hyacinth. As a result, plant extracts must be tested first, one of which is the viability test in accordance with the requirements and materials in the field of dentistry. The viability test is a cell-based test that is often used for screening compounds to determine whether the test compound has an effect on cell proliferation or has a direct cytotoxic effect that leads to cell death. The goal of this study is to figure out what concentration of water hyacinth leaf extract can keep human gingival fibroblast cells alive for 24 hours. Primary cell cultures from human gingiva were extracted and placed in a 96-well microplate. For 24 hours, water hyacinth leaf extract at concentrations of 1 mg/ml, 0.5 mg/ml, 0.25 mg/ml, 0.25 mg/ml, 0.125 mg/ml, 0.0625 mg/ml, 0.0312 mg/ml, 0.0156 mg/ml was administered to each well in the microplate. After 24 hours of incubation, the MTT assay was carried out by adding MTT solution. The optical density of formazan was measured using an ELISA reader at a wavelength of 590 nm, and viability was calculated using the viability formula. Starting at 0.125 mg/ml, 0.0625 mg/ml, 0.0312 mg/ml, and 0.0156 mg/ml, the vitality of human gingival fibroblast cells was good. In the treatment group, the greatest vitality of human gingival fibroblast cells was 0.0156 mg/ml (75.98%).
Effect Of Exposure Of Monosadium Glutamate (MSG) on Viability Of Monocyte Cells Aliza Dewi Fortuna
Journal of Stem Cell Research and Tissue Engineering Vol. 5 No. 2 (2021): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v5i2.33144

Abstract

The consumption rate of Monosodium Glutamate (MSG) in Indonesia has increased every year. Uncontrolled use of MSG in Indonesia for a long period of time can cause toxic effects on the body. The free glutamate content produced by MSG can affect the work of the immune system, especially in the innate immune system and cause oxidative stress. To determine the effect of exposure to Monosodium Glutamate (MSG) on the viability of monocyte cells. This study is a laboratory experimental in vitro with a post test only control group design. A total of 10cc of peripheral venous blood was isolated using the ficoll gradient centrifugation method. The results of monocyte cell isolates were exposed to Monosodium Glutamate (MSG) according to groups. Group I: negative control, group II: monocyte cells + MSG 3%, group III: monocyte cells + MSG 6%, group IV: monocyte cells + MSG 9%. Subsequently incubated for 24 hours at 37 °C in 5% CO2. Then the viability test was carried out using trypan blue staining. Monocyte cell viability calculations were carried out under an inverted microscope with a magnification of 400x per 100 cells. The data obtained were analyzed statistically using the one-way Anova test followed by the LSD test. The average viability in each group was obtained as follows, monocyte cell viability in the control group was 63%, group II was 47%, group III was 45% and group IV was 35%. There is an effect of exposure to Monosodium Glutamate (MSG) on the viability of monocyte cells with the most significant effect being the 9% MSG concentration with an average viability of 35%.The consumption rate of Monosodium Glutamate (MSG) in Indonesia has increased every year. Uncontrolled use of MSG in Indonesia for a long period of time can cause toxic effects on the body. The free glutamate content produced by MSG can affect the work of the immune system, especially in the innate immune system and cause oxidative stress. To determine the effect of exposure to Monosodium Glutamate (MSG) on the viability of monocyte cells. This study is a laboratory experimental in vitro with a post test only control group design. A total of 10cc of peripheral venous blood was isolated using the ficoll gradient centrifugation method. The results of monocyte cell isolates were exposed to Monosodium Glutamate (MSG) according to groups. Group I: negative control, group II: monocyte cells + MSG 3%, group III: monocyte cells + MSG 6%, group IV: monocyte cells + MSG 9%. Subsequently incubated for 24 hours at 37 °C in 5% CO2. Then the viability test was carried out using trypan blue staining. Monocyte cell viability calculations were carried out under an inverted microscope with a magnification of 400x per 100 cells. The data obtained were analyzed statistically using the one-way Anova test followed by the LSD test. The average viability in each group was obtained as follows, monocyte cell viability in the control group was 63%, group II was 47%, group III was 45% and group IV was 35%. There is an effect of exposure to Monosodium Glutamate (MSG) on the viability of monocyte cells with the most significant effect being the 9% MSG concentration with an average viability of 35%.
Comparison Between RunX2 and Osteocalcin Expression Following the Application of Demineralized Freeze-Dried Bone Xenograft (DBFX) and Bovine Hydroxyapatite (BHA) and Their Effect on Bone Defect (In vivo Laboratory Experiment) Ikhram Kharis
Journal of Stem Cell Research and Tissue Engineering Vol. 5 No. 2 (2021): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v5i2.33145

Abstract

The use of biomaterial such as bone graft material is highly needed in oral and maxillofacial surgery to overcome bone defect that happened due to various reasons. One of the bone graft that widely used is bovine hydroxyapatite (BHA). BHA is produced by means of deproteinizing by a high-temperature heating process so that inorganic material of bone is left where the bone architecture is preserved. This material has osteoconductive property because it induces osteoblast activity and new bone formation. DFDBX is a bone graft derived from bovine bone which has undergone the demineralization process and subsequently frozen. Then, it will be exposed to hydrochloric acid until the bone matrix component-related collagen fibril called BMPs. Runt-Related Transcription factor 2 (RUNX2) is a transcription factor which is needed for osteoblast differentiation and it is first detected at preosteoblast. Osteocalcin is exerted during the last stage of differentiation, started at the early stage of mineralization. Objectives to compare the expression of RUNX2 and Osteocalcin following the application of DFDBX and BHA to the bone defect. Methode 30 male New Zealand White Rabbit, 6- months old, 3-3,5kg, divided into 3 groups comprising of 10 animals each, bone defect is created on each animal model. On group 1, DFDBX is applicated, BHA is on group 2, and control group with no graft application. After 2 weeks and 4 weeks following the animal model is terminated to retrieve a bone specimens for Immunohystochemistry examination. Result The expression of RUNX2 following the application of DFDBX and BHA showed a significant difference at week 2 but not showed at week 4. This research also found that the expression of osteocalcin did not show a significant difference at week 2 but showed a significant difference at week 4. Conclussion This study demonstrate that bone healing process in DFDBX group is more effective than BHA.
Osteoinduction Ability Of Human Adiposed Derived Mesenchymal Stem Cell (HADMSC) with Chitosan Scaffold Combination Towards Blood Serum Phosphorus Levels Nindya Rizqi Anjani
Journal of Stem Cell Research and Tissue Engineering Vol. 5 No. 2 (2021): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v5i2.33146

Abstract

Reconstruction of extensive bone tissue damage is a treatment with complication. Because moving the autologous tissue such as bone graft can cause complications that causes problems in the repair of extensive tissue damage so, the principle of tissue engineering (stem cells, bioreactor / growth factor, and scaffold) is used as an alternative to reconstruct damage to the tissue because it has many advantages. The combination of hADMSC and chitosan scaffold, is expected to trigger osteoinduction that can be expressed by osteogenic markers such as phosphorus levels in blood serum. To prove osteoinduction in a combination of Human Adiposed Derived Mesenchymal Stem Cell (hADMSC) and chitosan scaffold using blood serum phosphorus levels. This study used 12 groups with 5 sample each. Groups 1 to 4 were the negative control group at day 1,3,7, and 14. While groups 5 to 8 were the positive control group at day 1,3,7, and 14. Groups 9 to 12 were treatment groups at day 1,3,7, and 14. In the negative control group bone was only removed, in positive control group, bone was removed and chitosan scaffold was added, and in treatment group, bone was removed then, hADMSC and chitosan scaffold combination was added . Blood collection will be carried out in each group for examination of phosphorus levels in the blood serum. There were differences in phosphorus levels in blood serum in each group even though statistically there were only significant differences on day 14. The combination of hADMSC and chitosan scaffold caused a significant change in blood serum phosphorus levels on day 14 which means it triggers osteoinduction.
Effect of Concentration of Mangrove Leaf Extract Lumnitzera Racemosa on Hela Cell Viability Ayu Kartika Fitri Ayu
Journal of Stem Cell Research and Tissue Engineering Vol. 6 No. 1 (2022): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v6i1.37511

Abstract

Cervical cancer is a disease caused by a malignant process that occurs in the cervix or cervix. The cause of cervical cancer is not known for certain, but it is estimated that around 95% is caused by HPV (Human Papilloma Virus). Efforts to cure cancer with drugs (pharmacotherapy) or with chemical compounds (chemotherapy) in general have not been able to give satisfactory results, so alternative treatment methods are sought, including traditional medicine, namely by using mangroves. Lumnitzera racemosa is one type of mangrove plant that has been used in alternative medicine because of its potential as anticancer. The aim of this study was to determine the effect of Lumnitzera racemosa mangrove extract on hela cell viability. Lumnitzera racemosa leaf powder was extracted using graded maceration. The solvents used include n-hexane, ethyl acetate, and ethanol. The results showed that the LC50 value was 56 ppm, it means that the ethanol extract has toxic properties. The results of the phytochemical test of the leaf extract of Lumnitzera racemosa contained alkaloids, steroids, triterpenoids and saponins. The test results showed that the extract yield was 11.58%, the water content of the extract was 22.17%, and the total phenol was 2742.17 mg GAE. The test results from the LC-MS test resulted in suspected compounds including pyrogallol, isoniazid and caffeine. The ethanolic extract of Lumnitzera racemosa leaf was cytotoxic to the viability of hela cells with the resulting IC50 value of 493.33 µg/mL.

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