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Journal of Stem Cell Research and Tissue Engineering
Published by Universitas Airlangga
ISSN : 26141264     EISSN : 26141256     DOI : https://dx.doi.org/10.20473/jscrte
Core Subject : Health, Science, Engineering,
Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary
Journal of Stem Cell Research and Tissue Engineering (JSCRTE) is published by Stem Cell Research and Development Center, Airlangga University. Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancerstem cells, developmental studies, genomics and translational research. Special focus of JSCRTE is on mechanisms of pluripotency and description of newly generated pluripotent stem cell lines. Articles that go through the selection process will be review by peer reviewer or editor. The journal is published regularly twice a year in December and May. Every publication consists of 60-70 pages and 5 scientific articles in the form of research, study literature, and the case study in English. The contributors Journal of Stem Cell Research and Tissue Engineering are Stem Cell researchers, lecturers, student and practitioners that came from Indonesia and abroad.
Arjuna Subject : Kedokteran - Imonologi dan Alergi
Articles 95 Documents
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THE EFFECTIVENESS OF STEM CELL SECRETOME ON POST-TOOTH EXTRACTION WOUND HEALING Depi Lusianti; Wasis Syahwanda
Journal of Stem Cell Research and Tissue Engineering Vol. 10 No. 1 (2026): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v10i1.92273

Abstract

Tooth extraction is a common surgical procedure performed in dental practice and may cause damage to both soft tissue and alveolar bone. The post-tooth extraction wound healing process involves various biological mechanisms, including inflammation, cell proliferation, angiogenesis, and tissue remodeling. In recent years, stem cell secretome has emerged as a promising regenerative therapy approach because it contains various bioactive factors such as growth factors, cytokines, and extracellular vesicles that play important roles in tissue repair. This study aimed to evaluate the effectiveness of stem cell secretome on post-tooth extraction wound healing through a systematic review method. Literature searching was conducted using the PubMed, Google Scholar, and ScienceDirect databases within the 2020–2026 publication period. Articles were selected based on inclusion and exclusion criteria according to the PRISMA guidelines. The review results showed that stem cell secretome was able to enhance fibroblast proliferation, angiogenesis, collagen deposition, wound epithelialization, and alveolar bone regeneration. In addition, secretome also played a role in reducing inflammatory responses, thereby accelerating the oral wound healing process. Based on the results of this systematic review, stem cell secretome has effective potential as a regenerative therapy to accelerate post-tooth extraction wound healing. However, further clinical studies are still needed to confirm its effectiveness and safety in humans.
ANTIBACTERIAL EFFECTS OF MESENCHYMAL STEM CELL SECRETOME ON THE GROWTH OF PATHOGENIC BACTERIA Parti Putri; Tri Juanda
Journal of Stem Cell Research and Tissue Engineering Vol. 10 No. 1 (2026): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v10i1.92276

Abstract

Bacterial infection remains a major global health problem due to the increasing emergence of antibiotic-resistant microorganisms. Mesenchymal stem cell (MSC) secretome has attracted attention as a potential cell-free therapeutic approach because it contains bioactive molecules with antimicrobial, immunomodulatory, and regenerative properties. This study aimed to evaluate the antibacterial activity of MSC secretome against pathogenic bacteria in vitro. This experimental laboratory study used a post-test only control group design. Human dental pulp-derived MSCs were cultured under standard conditions, and conditioned medium containing secretome was collected and processed. Antibacterial activity against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa was evaluated using agar diffusion and bacterial viability assays. Experimental groups included control, low-, medium-, and high-concentration secretome treatments. Inhibition zone diameters and Log10 CFU/mL values were analyzed using one-way ANOVA followed by Tukey post hoc test with a significance level of p < 0.05. The results showed that MSC secretome significantly inhibited bacterial growth compared with the control group. Antibacterial activity increased proportionally with secretome concentration, as demonstrated by larger inhibition zones and lower bacterial viability values. The highest antibacterial activity was observed in the high-concentration secretome group, which reduced bacterial viability by approximately 70–80%. Staphylococcus aureus showed greater susceptibility than Gram-negative bacteria, particularly Pseudomonas aeruginosa. Statistical analysis confirmed significant differences among treatment groups (p < 0.05). In conclusion, MSC secretome demonstrated significant concentration-dependent antibacterial activity against pathogenic bacteria in vitro. These findings suggest that MSC secretome may serve as a promising cell-free therapeutic strategy for infectious disease management and regenerative medicine.
EFFECT OF FETAL BOVINE SERUM (FBS) CONCENTRATION ON THE VIABILITY AND PROLIFERATION OF MESENCHYMAL STEM CELLS (MSCS) IN VITRO Tri Juanda; Parti Putri
Journal of Stem Cell Research and Tissue Engineering Vol. 10 No. 1 (2026): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v10i1.92278

Abstract

Mesenchymal Stem Cells (MSCs) are multipotent stem cells widely utilized in regenerative medicine due to their self-renewal and differentiation capacities. The success of MSC expansion in vitro is strongly influenced by culture conditions, particularly the concentration of Fetal Bovine Serum (FBS) used in the culture medium. This study aimed to evaluate the effect of different FBS concentrations on the viability and proliferation of MSCs in vitro. An experimental laboratory study with a post-test only control group design was conducted using MSC cultures supplemented with 0%, 5%, 10%, and 15% FBS in Dulbecco’s Modified Eagle Medium (DMEM). Cells were incubated for 72 hours under standard culture conditions. Cell viability was assessed using the MTT assay, while proliferation was analyzed using trypan blue exclusion and hemocytometer counting. Statistical analysis was performed using One-Way ANOVA followed by Tukey post hoc test with a significance level of p < 0.05. The results demonstrated that FBS concentration significantly affected MSC viability and proliferation. MSCs cultured with 10% FBS exhibited the highest viability, proliferation rate, and optimal spindle-shaped fibroblast-like morphology with high confluency. In contrast, cells cultured without FBS showed poor attachment, reduced proliferation, and increased apoptotic features. Although the 15% FBS group maintained relatively high viability, proliferation was slightly lower compared to the 10% FBS group. In conclusion, FBS concentration plays a critical role in maintaining MSC growth and survival in vitro, with 10% FBS providing the most optimal culture condition among the tested groups.
IDENTIFICATION OF KEY GENES AND MOLECULAR PATHWAYS ASSOCIATED WITH OSTEOGENIC DIFFERENTIATION OF DENTAL PULP STEM CELLS Wasis Syahwanda; Elsa Fitri
Journal of Stem Cell Research and Tissue Engineering Vol. 10 No. 1 (2026): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v10i1.92286

Abstract

Dental pulp stem cells (DPSCs) have emerged as promising candidates for regenerative dentistry due to their self-renewal capacity and multilineage differentiation potential, particularly in osteogenic differentiation. Understanding the molecular mechanisms underlying this process is essential for developing effective regenerative therapies. This study aimed to identify key genes and molecular pathways involved in the osteogenic differentiation of DPSCs using a bioinformatics-based in silico approach. Gene expression data were obtained from the Gene Expression Omnibus (GEO) database, specifically the GSE80614 dataset, which compared undifferentiated and osteogenically induced human DPSCs. Differentially expressed genes (DEGs) were identified using GEO2R with the criteria of p-value < 0.05 and |log fold change| ≥ 1. Functional enrichment analysis was conducted using DAVID, while protein–protein interaction (PPI) networks were analyzed using STRING and Cytoscape. The analysis revealed distinct transcriptional differences between undifferentiated and osteogenically differentiated DPSCs. Functional enrichment demonstrated significant involvement of biological processes related to extracellular matrix organization, ossification, and regulation of cell differentiation. Key signaling pathways identified included PI3K-Akt signaling, TGF-β signaling, ECM–receptor interaction, and focal adhesion pathways, all of which are closely associated with osteogenesis. PPI network analysis identified several hub genes, including RUNX2, ALPL, BMP2, and COL1A1, suggesting their central regulatory roles in osteogenic differentiation. Overall, this study provides a systematic overview of the molecular mechanisms associated with DPSC osteogenesis and highlights potential therapeutic targets for regenerative dental applications. Further experimental validation is required to confirm these findings.
OPTIMIZATION OF CULTURE AND CHARACTERIZATION METHODS OF DENTAL PULP STEM CELLS FOR REGENERATIVE APPLICATIONS IN DENTISTRY Yulda Ningsih; Depi Lusianti
Journal of Stem Cell Research and Tissue Engineering Vol. 10 No. 1 (2026): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v10i1.92294

Abstract

Dental pulp stem cells (DPSCs) are a type of mesenchymal stem cell with high potential in the field of regenerative dentistry. Their successful application is greatly influenced by the methods used for isolation, culture, and characterization; however, to date, there is no fully agreed-upon standard. This article aims to review and optimize the methods of isolation, culture, and characterization of DPSCs based on the latest scientific literature in order to obtain the most effective and consistent approach for laboratory and regenerative application. This article is a methodological paper based on a literature review (2020–2025) from PubMed and Google Scholar. The protocol was developed through the synthesis of various studies related to enzymatic isolation and the explant method, cell culture using DMEM or α-MEM media, and characterization using flow cytometry and immunocytochemistry. The analysis was conducted descriptively and narratively to compare the effectiveness of each method. The enzymatic method demonstrates higher efficiency in cell isolation, whereas the explant method provides more stable cell viability. Cell culture using media supplemented with fetal bovine serum (FBS) under conditions of 37°C and 5% CO₂ supports optimal proliferation. Characterization using markers CD73, CD90, and CD105 (positive), as well as CD34 and CD45 (negative), constitutes the primary standard for DPSC identification. The combination of enzymatic isolation methods, controlled culture using standard media, and characterization based on flow cytometry represents the most optimal approach for producing high-quality DPSCs for regenerative dentistry applications.

Page 10 of 10 | Total Record : 95

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