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Journal of Microbial Systematics and Biotechnology

jmsb
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Published by Cibinong Science Center

ISSN : - EISSN : 26854430 DOI : https://doi.org/10.37604/jmsb

Core Subject : Science,

Immunology & microbiology

Journal of Microbial Systematics and Biotechnology (JMSB) is the international journal of microbial diversity and microbial technology which publishes research articles, reviews, and methodologies of microbial-based technology; and taxonomic articles such as monographs, new species, new notes, new records, checklists related to microbial diversity. The official language is English. Every manuscript submitted to JMSB will be published as soon as the editor receives it, and through the peer review process.

Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi

Articles 7 Documents

Search results for , issue "Vol 1, No 1 (2019): June 2019" : 7 Documents clear

PCR-based Specific Detection of Bacillus in Liquid Organic Fertilizer Iman Hidayat; Nur Laili; Dwi Agustiyani; Sarjiya Antonius
Journal of Microbial Systematics and Biotechnology Vol 1, No 1 (2019): June 2019
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Rapid molecular PCR-based detection method for Bacillus species used in the production of Beyonic® liquid organic fertilizer was carried out based on nucleotide sequence data from the 16S rRNA gene. The method involved sequencing the 16S rRNA gene of several Bacillus species and identifying around 16-22 specific nucleotide bases from 5' and 3' ends in the Bacillus 16S rRNA gene sequences. One specific primer pair for Bacillus detection was determined as follow: 5' - CAT AAG ACT GGG ATA ACT CCG GG - 3' (forward) from positions of 85-107 bp, and 5’ - CCA GGC GGA GTG CTT AAT GC - 3’ (reverse) from positions of 836-854 bp. PCR assay and gel electrophoresis analysis showed that the primer pair was specific to the genus Bacillus.

Cover JMSB Vol 1, No 1 (2019) JMSB JMSB
Journal of Microbial Systematics and Biotechnology Vol 1, No 1 (2019): June 2019
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Biosorption chrome (Cr) and dyes using biosorbent in the modified tea bag Sri Lestari; Ratna Stia Dewi; Eko Setio Wibowo; Atang `
Journal of Microbial Systematics and Biotechnology Vol 1, No 1 (2019): June 2019
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Biosorption has been known as a potential method in reducing heavy metals and dyes in wastewater, including chrome (Cr) and dyes contents of the wastewater from the batik industry. Straw and spent mushroom compost (SMC) are potential biosorbents due to cheap, abundant, and easily obtained. However, the effectivity of the biosorbents is not only depended upon the type of material but also their surface area. In this study, a modified tea bag was used to increase the surface area of the straw and spent mushroom compost to absorb Cr and dyes from the batik industry wastewater. The optimum of biosorbent ratio and pH in Cr absorption and dyes decolorization was measured. This experiment was conducted using Spilt Plot Design. The results showed that the highest Cr absorption was found at 0,0050 mg/g, and the percentage of decolorization was 68,92% in the biosorbent ratio of 3:1 and pH 5. Biosorbent packed in the tea bags modification was effective removes Cr and dyes in the batik industry wastewater.

Antagonistic activity of three Aspergillus isolates against Fusarium wilt of banana Iman Hidayat; Lita Citra Dewi; Dalia Sukmawati
Journal of Microbial Systematics and Biotechnology Vol 1, No 1 (2019): June 2019
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Endophytic fungi have been known to play a role in protecting their host plants against diseases and extreme environments. The purpose of this study was to determine the antagonistic activity of three Aspergillus isolates against F. oxysporum f. sp. cubense (Foc), and to identify the Aspergillus identity using multilocus phylogeny analysis. Antagonistic assay by dual culture method showed that the Aspergillus sp. strain PD2, strain PD4, and strain PD5 inhibited the growth of Foc isolate by 37.31%, 26.52%, and 12.04%, respectively. Multilocus phylogeny based on ITS rDNA, beta-tubulin, and calmodulin genes sequences showed that the Aspergillus strain PD2 and strain PD4 belong to A. section Terrei, while the Aspergillus sp. strain PD5 was identified as A. sydowii of the A. sect. Versicolores.

Inventory of macrofungi at peat swamp forest area, Kapuas Hulu, West Kalimantan Noverita Noverita; Tatang Mitra Setia
Journal of Microbial Systematics and Biotechnology Vol 1, No 1 (2019): June 2019
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

The areas surrounding peat swamp forest at Dusun Meliau and Bukit Peninjau, Kapuas Hulu, West Kalimantan host a diverse species of flora and fauna, including macrofungi. However, the information on the macrofungal diversity in this area has not been reported. The goal of this study was to obtain information on the diversity of macrofungi that can be used as food and medicine. Data was collected by direct modified collection method using plot (10 × 20 m) with interval 10 m along the transect line. A total of 79 specimens of macrofungi discovered from the two locations, of which 23 and 66 specimens were collected from Dusun Meliau and Bukit Peninjau, respectively. Of the 79 macrofungi specimens, 16 specimens were potential as a food source, and 41 as medicine. The macrofungi in this study was dominated by members of Ordo Aphyllophorales.

Antibiofilm and antimicrobial activities of papaya (Carica papaya L.) and stevia (Stevia rebaudiana Bertoni) leaf extracts against three biofilm-forming bacteria Aerma Hastuty
Journal of Microbial Systematics and Biotechnology Vol 1, No 1 (2019): June 2019
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Biofilm is a structural form of a microbial group that is protected by the Extracellular Polymeric Substance (EPS) matrix. The biofilm is considered as the main mediator of infection, and plays a major role in the occurrence of drug resistance. This study was aimed at determining the antimicrobial and antibiofilm activities of papaya (Carica papaya L.) and stevia (Stevia rebaudiana Bertoni) leaf extracts against three biofilm-forming bacteria. The antimicrobial assay showed that papaya leaf extract exhibits higher activity compared to stevia leaf extract in inhibiting the growth of the biofilm-forming bacteria. The optimum condition of papaya leaf extract to inhibit biofilm-forming bacterial growth occurred at 45% and 75% concentrations of the extract (pH 7). A 100% biofilm degradation by papaya leaf extract occurred at pH 6 and pH 9.

Nickel (Ni) reduction in Sorowako post-mining soil through application of mycorrhiza Acaulospora sp. associated with Canavalia ensiformis L. M. Akhsan Akib; Kahar Mustari; Tutik Kuswinanti; Syatrianty Andi Syaiful; Syatrawati `; Z. Kumalawati
Journal of Microbial Systematics and Biotechnology Vol 1, No 1 (2019): June 2019
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

The nickel (Ni) content in a post-mining soil of Pomalaa mines reached 14,200 mg.kg-1 and became a limiting factor in the plant growth process. A Ni reduction in the soil by using phyto-accumulator such as Jack bean (Canavalia ensiformis L.) can be improved by combining it with arbuscular mycorrhizal (AM) fungi. The purpose of this study was to determine the effect of the mycorrhizal fungus Acaulospora sp. on the efficiency of Ni reduction by C. ensiformis. This experiment was carried out by using a randomized block design with three different treatments, include: 1) C. ensiformis without Acaulospora sp. inoculation (negative control), 2) C. ensiformis inoculated with indigenous Acaulospora sp. and 3) C. ensiformis inoculated with non-indigenous Acaulospora sp. The study was conducted in the nursery that belongs to PT. Vale Indonesia Tbk., Sorowako, South Sulawesi, Indonesia. The results showed that highest nickel accumulation was found in the root inoculated with indigenous Acaulospora sp. (9500 mg.kg-1), followed by stem (1400 mg.kg-1), leaf and pod (1300 mg.kg-1), seed (1200 mg.kg-1), and flower (1100 mg.kg-1). This study indicates that application of the indigenous Acaulospora sp. can improve C. ensiformis efficiency to reduce Ni content at Sorowako post-mining area.

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Editorial Team

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Contact Name
Debora Christin Purbani

Contact Email
editor@e-jmsb.id

Phone
+6221-8761356

Journal Mail Official
editor@e-jmsb.id

Editorial Address
Jln. Raya Jakarta-Bogor KM. 46 Cibinong 16911, West Java, Indonesia

Location
Kota bogor,

Jawa barat

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Debora Christin Purbani

Contact Email editor@e-jmsb.id

Phone +6221-8761356

Journal Mail Official editor@e-jmsb.id

Editorial Address Jln. Raya Jakarta-Bogor KM. 46 Cibinong 16911, West Java, Indonesia

Location Kota bogor, Jawa barat INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Debora Christin Purbani

Contact Email
editor@e-jmsb.id

Phone
+6221-8761356

Journal Mail Official
editor@e-jmsb.id

Editorial Address
Jln. Raya Jakarta-Bogor KM. 46 Cibinong 16911, West Java, Indonesia

Location
Kota bogor,

Jawa barat

INDONESIA